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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 6 Documents
 1 
Search results for , issue "Vol 5, No 1 (2025)" : 6 Documents clear
Role of Organic and Phosphate Fertilizer on Growth and Yield of Groundnut Supriyono Supriyono; Cholis Laila Aryani; Pardono Pardono; Maria Theresia Sri Budiastuti
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.111404

Abstract

Efforts to increase groundnut productivity can be achieved by fertilizing with organic and phosphate fertilizers. This research aims to examine the role of organic and phosphate fertilizers on growth and yield of groundnut. The research was conducted at the Faculty of Agriculture UNS Jumantono, EMPT (Ecology and Plant Production Management) Laboratory, Soil Chemistry and Fertility Laboratory from December 2024 to March 2025. The research used a Randomized Complete Block Design (RCBD) with two factors and each repeated three times. The first factor is dose of organic cow manure fertilizer (0, 10, 20, 30 ton.ha-1) and second factor is dose of phosphate fertilizer (0, 75, 150, 225 kg.ha-1). The observed variables include initial soil analysis, growth, yield, plant tissue analysis, and nutrient uptake analysis. Observational data were analyzed using analysis of variance (ANOVA) at a 95% confidence significant, Duncan Multiple Range Test (DMRT) at a 95% confidence significant, regression tests, and Pearson correlation tests. The results showed that cow manure fertilizer with an optimum dose of 25.67 ton.ha-1 combined with 225 kg.ha-1 of phosphate fertilizer resulted in the early flowering age, namely 24.97 days after planting. Organic cow manure fertilizer 10 ton.ha-1 was able to increase the number of leaves, leaf area index, fresh weight of straw, number of pods, dry weight of pods, number of seeds per plant, and seed weight per plant of groundnut compared without organic cow manure fertilizer. Phosphate fertilizer 75 kg.ha-1 was able to increase the number of pods, dry weight of pods, and number of seeds per plant compared without phosphate fertilizer.
Potential of Trichocompost to Improve Organic Garlic Production in Fusarium Wilt–Endemic Fields in Tawangmangu, Central Java Susilo Hambeg Poromarto; Supyani Supyani; Salim Widono; Dwiwiyati Nurul Septariani; Hadiwiyono Hadiwiyono; Argha Hyta Dimas Enggartiasto
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.111410

Abstract

Garlic is a high-value commodity in Tawangmangu, one of its major production centers in Central Java. However, productivity in this region is seriously affected by Fusarium, the pathogen causes basal rot and wilting in the upper parts of the plant. The disease known as basal rot or Fusarium wilt, with the disease intensity in the field can exceeding  60%. Currently, chemical control remains the primary management strategies, which in organic farming, this practice is not acceptable. Therefore, environmentally friendly alternatives are required, such as using organic fertilizers, biological control agent like Trichoderma or combination. Trichoderma is also known as a cellulolytic microbe, which can enhance the composting process and produce Trichompost with improved quality. Field experiment results indicate that Trichocompost (TC) performs better compared to compost (C), Trichoderma (T), or untreated treatment. This findings suggest that garlic treated with Trichocompost and organic fertilizer show better growth with lower wilting intensity. Therefore, there is an indication that increased plant growth can reduce the intensity of Fusarium wilt in garlic.
In Vitro Effects of Yeast Extract and Indole-3-Acetic Acid on Shoot Emergence and Height in Mas Kirana Banana Samanhudi Samanhudi; Retna Bandriyati Arniputri; Sri Hartati; Iswahyudi Iswahyudi; Dewi Selvia Maulida Saputri
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108197

Abstract

Mas Kirana banana (Musa sp.) is a superior local variety from Lumajang, East Java, valued for its favorable fruit size and consumer preference. Conventional propagation of this variety is limited due to its low production of saplings (2–3 per clump), making large-scale cultivation inefficient. Tissue culture techniques offer an alternative approach to accelerate propagation. This study aimed to evaluate the effects of yeast extract and indole-3-acetic acid (IAA) on the in vitro growth of Mas Kirana banana explants. The experiment was arranged in a factorial completely randomized design (CRD) with two factors: yeast extract at four concentrations (0, 400, 800, and 1200 mg L⁻¹) and IAA at four concentrations (0, 0.75, 1.5, and 2.25 ppm). Growth responses were assessed based on shoot emergence, shoot height, root development, and leaf formation. Yeast extract significantly affected shoot emergence time; however, its effect was inhibitory rather than promotive. The control (0 mg L⁻¹) produced the fastest emergence (11 DAP), whereas higher yeast concentrations (400–1200 mg L⁻¹) delayed emergence to 13–22 DAP.. IAA significantly influenced shoot height, with the best performance at 1.5 ppm, while other growth parameters were not significantly affected. These findings indicate that the effectiveness of yeast extract and IAA is highly concentration-dependent, and their roles in improving micropropagation of Mas Kirana banana require further optimization. This study provides baseline information to support the development of more efficient tissue culture protocols for large-scale propagation of this important local variety.
The Effect of Naphtaleine Acetic Acid ND Benzyl Amino Purine on the Subculture of Kepok Unti Sayang Banana Samanhudi Samanhudi; Andriyana Setyawati; Edi Purwanto; Muji Rahayu; Eviani Eviani; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108513

Abstract

The Kepok Unti Sayang variety is a superior type of banana that is resistant to fusarium wilt. The high demand for Kepok Unti Sayang needs to be balanced with good seed production. One of the methods used for multiplying superior seeds of Kepok Unti Sayang is through tissue culture. This research aims to obtain the optimal concentration of Naphtaleine Acetic Acid (NAA) in the subculture media for the growth of root numbers in the Kepok Unti Sayang Banana. The second objective is to determine the optimal concentration of Benzyl Amino Purine (BAP) in the subculture medium for shoot number growth in Kepok Unti Sayang bananas. Another goal is to identify the best treatment combination that can produce the highest number of shoots in Kepok Unti Sayang bananas. The research was conducted at the Laboratory of Plant Physiology and Biotechnology at Sebelas Maret University from February to July 2025. The study was conducted with 4 levels of NAA treatment: 0; 0.5; 1; and 1.5 ppm. The BAP treatment was administered at 4 levels: 0; 1; 2; and 3 ppm. The variables observed in this study were the time of shoot emergence, number of shoots, time of root emergence, root length, and final height of the planlet. Data from each variable were then analyzed using analysis of variance (ANOVA) with an F-test at a significance level of 95%. Significantly different data were then followed by Duncan's Multiple Range Test (DMRT) at a significance level of 95%. The significant combination of NAA and BAP on the observed variables was then reanalyzed using regression tests. The results showed that the application of various concentrations of NAA does not affect the growth of the number of roots of Kepok bananas var. Unti Sayang. The application of 2 ppm BAP is optimal for the growth of the number of shoots of Kepok bananas var. Unti Sayang. The interaction between 1 ppm NAA and BAP at 1-2 ppm produces the most optimal average shoot emergence time.
Isolation of Lactic Acid Bacteria from Soil and Its Antifungal Activity Against Aspergillus flavus Athiyyah Jihan Tifani; Tjahjadi Purwoko; Ari Susilowati
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.109898

Abstract

Food stocks, such as grains, fruits, and vegetables are vulnerable to spoilage by Aspergillus flavus, which typically controlled using chemical preservatives. Concerns about the health impact of these chemicals highlight the need for safer alternatives, such as biopreservatives. Lactic acid bacteria (LAB) are Generally Recognized as Safe (GRAS) microorganisms and have potential application as biopreservative agent, because LAB produced various metabolites with antifungal activities. LAB could be found in nutrient-rich environments, including rhizosphere soil and poultry house soil due to the nutrient residues for growth. The aims of this study were to isolate LAB from soils and to evaluate their antifungal activity with a focus on potential applications as biopreservatives. The LAB isolates from soil were characterized and screened for antifungal activity using the dual culture method. The LAB isolates inhibited A. flavus growth with the largest growth inhibition was showed by LAB isolate 1 that isolated from poultry house soil (17.65%). However, statistics analysis showed that fungal growth inhibition by LAB isolates were not significantly difference (p>0,05). Although the result was not statistically significant, all  LAB isolates significantly inhibited conidia development. This showed potential application of LAB isolates as biopreservative agent. Keywords: antifungal, Aspergillus flavus, biopreservative, food stock, lactic acid bacteria
Comparative Analysis of Bioacoustic and Point Count Methods for Calculating Bird Species Richness in Bukit Watu Ondo, Mount Ungaran, Central Java Divanda Lathiifanindya; Vika Widyaningrum; Yudistira Nurpratama; Maesa Faradina; Ni’mah Alawiyah Safitri; Margareta Rahayuningsih
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.110928

Abstract

The study aims to compare the effectiveness of bioacoustic and point count methods in assessing bird species richness in Bukit Watu Ondo, Mount Ungaran, Central Java. A quantitative approach was employed through simultaneous data collection using both methods at six observation points. Bird observations were conducted visually and acoustically for 15-minute sessions at each point, repeated three times. The data were analyzed using a paired t-test to determine statistical differences between the two methods. Results showed that the point count method identified 29 species from 22 families, while the bioacoustic method recorded 25 species from 17 families. Statistical analysis revealed no significant difference (p = 0.927) between the two methods in the number of detected species, indicating comparable effectiveness. However, bioacoustics proved more efficient in detecting vocally active or cryptic species that were difficult to observe visually, whereas point count performed better for visually conspicuous species. The combination of both methods provided a more comprehensive representation of avifaunal diversity. These findings demonstrate that bioacoustic techniques are a viable and complementary alternative to traditional visual methods in biodiversity monitoring and conservation management.

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