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<![CDATA[Daftar Isi GAMMA Volume 6 Nomor 1, September 2010 ]]>
<![CDATA[Daftar Isi]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[Karakter vegetatif dan produksi awal tanaman jarak pagar (jatropha curcas l.) Akibat perlakuanmutagen colchicineAgus ZainudinKarakterisasi Tepung Tapioka LaktatDamatPengujian Produksi Sel Debaryomyces sp. pada Beberapa Media untuk Pengendalian HayatiPenyakit Antraknosa pada CabaiDian IndratmiIdentifikasi dan Uji Kualitas Pigmen Kulit Buah Naga Merah (Hylocareus Costaricensis)pada Beberapa Umur Simpan dengan Perbedaan Jenis PelarutElfi Anis SaatiProfil Komponen Bioaktif Tanaman Kava-Kava (Piper Methysticum, Forst, F) dari BerbagaiLokasiElly PurwantiAktivitasAntibakteri Saponin Hasil Isolasi Aloe Barbadensis Miller Terhadap StaphylococcusAureus Penyebab Mastitis pada Sapi PerahImbang Dwi RahayuEfektivitas Salep Daun Sirih Dan Meniran Terhadap Penurunan Jumlah Bakteri pada SapiPerah Penderita Mastitis Sub KlinisLili ZalizarInduksi variasi somaklonal jarak pagar (jatropha curcas l.) Untuk mendapatkan Karaktertoleran cekaman kekeringanMaftuchahAktivitas Antimikroba Ekstrak Lidah Buaya (Aloe Barbadensis Miller) Terhadap BakteriPatogen pada IkanSri Dwi HastutiProfil Kandungan Metabolit Sekunder Tumbuhan Obat Biophytum Petersianuum danBiophytumsensitivumSukarsono]]>
<![CDATA[KARAKTERVEGETATIF DAN PRODUKSI AWAL TANAMAN JARAK PAGAR (JATROPHA CURCAS L.) AKIBAT PERLAKUAN MUTAGEN COLCHICINE ]]>
<![CDATA[Agus Zainudin]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
Publisher : <![CDATA[Jurnal Gamma ]]>
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<![CDATA[Mutation and polyploidization represent some of plant breeding method exploited to increasegenetic diversity of physic nut. The result of mutation polyploidization process obtainable superiorphysic nut polyploid. This research was aimed to study the appearance of physic nut effected byconcentration and frequency of colchicine treatment. The type of physic nut used in this research isIP-1A. The combination of concentration colchicine (0,1%; 0,2%; 0,3%; 0,4%; 0,5%; 0,6%) andfrequency of colchicine solution (6 times, 8 times, 10 times) treated to get a mutant of physic nut.Plant without colchicine as a control. The result of this research indicated that the colchicine causevariation at vegetatif character. There is 3-5 crops having vegetatif size higher than control crop.Treatment of colchicine 0,5% 6x; 0,3% 10x and 0,2% 8x yields higher maximum fruit that is 95-168 fruits compared to control crop yielding 89 fruits.Key words : Physic nut, polyploid, mutation, colchicines]]>
<![CDATA[KARAKTERISASITEPUNG TAPIOKA LAKTAT ]]>
<![CDATA[Damat .]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[Consumption of wheat flour in our country from year to year continue to increase. Ironicallyuntil now wheat flour as raw material still completely imports. In 2007 imports of wheat has reached4.4 million tons. So for those reasons, it is necessary to substitution of wheat flour with other flourof tubers are widely planted by our society. One source of potential tubers is starch that can beprocessed into starch. The major problem of tapioca flour has no gluten so is relatively difficult toexpand. For those reasons, need modification of starch.The general objective of this research is to find a method of starch synthesis of lactate byphysical and chemical characteristics of wheat flour-like characteristics. The specific objective ofthis research is to improve the physical and chemical characteristics of starch so that it hascharacteristics that resemble the characteristics of wheat flour. The results showed that thefermentation for 12 hours to 60 hours of power a real influence on the development of the flourproduced kasava. Additionally, improvement of physical and chemical properties of flour kasavaresult of natural fermentation treatment. This allows a variety of products can be made using rawmaterials from kasava flour fermentation. While the cake flour produced from the fermentationkasava not much different from the cakes made from flour or it can be said nearly matching thequality of wheat flour.]]>
<![CDATA[PENGUJIAN PRODUKSI SEL DEBARYOMYCES SP. PADA BEBERAPA MEDIA UNTUK PENGENDALIAN HAYATI PENYAKIT ANTRAKNOSA PADA CABAI ]]>
<![CDATA[Dian Indratmi]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[Result of research Dian Indratmi previously (2000, 2001, and 2002) indicated among antagonisfungi that shown potential inhibition Colletotrichum gloeosporioides better were fructoplant yeastinoculan especially Debaryomyces sp. For evaluation of efficacy yeast Debaryomyces sp.as apotential biokontrol agent of chilli anthracnose or to develop this agent as biopesticide, it is importantto have available culture media that maximize production at low cost.The aimed of this research to find out a low cost media culture formulation for yeast fructoplantbiomass production for their application on field to suppressed anthracnose disease on pepper.The laboratory experiment with Complete Randomized Design and 6 replication. There were11 treatment, it’s A = malt extract liquid media; B = malt extract liquid media + pepper extract; C= corn meal liquid media; D = corn meal liquid media + pepper extract; E = sugar potato extractliquid media; F = Alioshina liquid media; G = sugar mixture of rice and bran liquid media; H =sugar pepper extract liquid media; I = maize solid media; J = waste water rice media; K = ipomoealiquid media.Results of the experiment indicated 11 of the yeast media culture test can as a substrate foryeast biomass production. Yeast Debaryomyces sp that cultured on the substrate corn meal liquidmedia that added with or without pepper extract resulted better weight and quantity cell of yeastDebaryomyces sp. The yeast for their growing needed a rather acid substrate, it’s about pH 4.79-6.07.]]>
<![CDATA[IDENTIFIKASI DAN UJI KUALITAS PIGMEN KULIT BUAH NAGA MERAH (HYLOCAREUS COSTARICENSIS) PADA BEBERAPA UMUR SIMPANDENGAN PERBEDAAN JENIS PELARUT ]]>
<![CDATA[Elfi Anis Saati]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[An alternative excavation of natural materials that could potentially be used as a substancedyes, continue to be made, including the skin of red dragon fruit. Because the red dragon fruit latelya lot of public interest, skin which amounts to 30-35% is often just discarded as waste only.In an effort to use the red dragon fruit peel waste that is not optimal, to increase the economicvalue of the skins of red dragon fruit and power use for the community.The goal is to mengetehaui type of pigment and pigment quality of dragon fruit red on someshelf with different types of solvents.This research used Randomized Design Group (RAK) Factorial consisting of two factors I;comprised of over 4 levels; factor II consisted of 3 level. Factor I: The Fruit Store (S), fresh (day0), the shelf life of fruit 2 days, 4 days and 8 days, whereas the second factor: the type of solvent(L), namely aquades; combination aquades and citric acid (9:1), and a combination Eeanol 1N andcitric acid (9:1).The results showed that the skin of red dragon fruit (Hylocereus costaricensis) containinganthocyanin type ramnosil sianidin 3-glucoside 5 - glucoside, based on Rf values (retrogradationfactor) of 0.36 to 0.38 and maximum absorbance at a wavelength with ë = 536.4 nm. Combinationtreatment S2P2 (the shelf life of fruit 4 days with solvent water and citric acid) produce anthocyaninpigments of red dragon fruit skin with the best quality, with a pH value of 1.91; the brightness (L)25.60; level of redness (a +) 6.97; the yellow (+ b) 0.50, 0.363 pigment absorbance; levels ofanthocyanin 1.1 mg/100ml; total dissolved solids 66.52% and 10.02% yield.]]>
<![CDATA[PROFIL KOMPONEN BIOAKTIF TANAMAN KAVA-KAVA (PIPER METHYSTICUM, FORST, F) DARI BERBAGAI LOKASI ]]>
<![CDATA[Elly Purwanti]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[The purpoeof this research is to know the profil of bioactive component from root, stem,leaves of cava-cava and the information of which part of plant that give the effect of locomatioeffect and antidepressive by using parameter of immobility duration with swimming method andsleeping duration.Method used in evaluating quality and standart of plant that potentially as herbal medicinethat usually used and information is Thin layer Chromatography (TLC) and High Speed LiquidChromatography (HPLC) ( dashek 1997) Herbal plant that conatins some variuos substances cansupport each other but it can possibly has antagonist effect. This the reason why it is important toknow which part of the plant is advantagous and which part of this plant as the source ( root, stem,leaf, seed)The extract result comparative between leaves organ and root organ and then to evaluationbase on thin layer chromatografi (TLC) each organ is dominant found Flavokawain, MethysticindanYangonin. The profil organic acid from root and leaves found the same and equal organic acidthat is benzoate acid. Soil type Malang is andosol, kambisol, latosol, alluvial and Soil type Mojokerto62,7% is Alluvial, 37,16% grumosol with rich mineral. Base on etanol exctraction , result thatcontent of bioactive component dominant from plant grow in type soil alluvial.]]>
<![CDATA[AKTIVITASANTIBAKTERI SAPONIN HASIL ISOLASI ALOE BARBADENSISMILLERTERHADAPSTAPHYLOCOCCUSAUREUS PENYEBAB MASTITIS PADA SAPI PERAH ]]>
<![CDATA[Imbang Dwi Rahayu]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[The mastitis in dairy cattle is caused by Staphylococcus aureus. The bacteria are multiresistantto antibiotic. Isolated of Aloe barbadensis Miller, that has antibacterial, saponin is needed to replacesynthetic antibiotic. The research has conducted to tested antibacterial activity of saponin for thegrowth of Staphylococcus aureus.Saponin was exstracted from gel of Aloe vera leafs by n-hexane and methanol. Isolated ofsaponin used to the Coulom Cromatography and Thin Layer Cromatography. It needs H2SO4 foridentifying of saponin. The saponin’s powder was produced by drying process, amylum or dexstrinas kinds of filler. The ratio of saponin and amylum or dexstrin was 80% : 20%. Six levels of saponinwere used in experiment i.e : 1,5%; 3,0%; 6,2%; 12,5%; 25% and 50%. The parameters of theresearch were Minimum Inhibition Concentration (MIC) and cakram disk. The result of experimentwas analysed with descriptively, penicillin was used as positive control.The saponin’s powder give antibacterial activity that higher than saponin’s concentrated. Therewere similarity in antibacterial activity between the saponin’s powder was added amylum withpenicillin.]]>
<![CDATA[EFEKTIVITAS SALEP DAUN SIRIH DAN MENIRAN TERHADAP PENURUNAN JUMLAH BAKTERI PADA SAPI PERAH PENDERITA MASTITIS SUB KLINIS ]]>
<![CDATA[Lili Zalizar]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[The study aimed to examine the effectiveness of daun sirih (Piper betle leaf ) and meniran(Phyllanthus niruri) ointment to decrease the number of bacteria in milk of subclinical mastitis ofdairy cows have been conducted on a dairy farm in Pujon and in the Faculty of Agriculture-AnimalHusbandry Muhammadiyah University of Malang. Experiment has done with completely randomizeddesign and five treatments: group negative controls (given sterile Nacl), positive control (givenantiseptic iodine), Piper betle leaf ointment, Phyllanthus niruri ointment and mixed ointment (Piperbetle and Phyllanthus niruri) leaves mixed with a ratio 50 percent each herbal. Results showed thenumber of bacteria in group treated with herbal ointment fewer than the group without treatment.The number of bacteria on day 20 (end of study) decline of 90.35 percent in dairy cow with havePiper betle leaf ointment; 87.92 percent with meniran ointment and 98.86 percent of the grouphave mixed (Piper betle leaf and Phyllanthus niruri) ointment and the group receiving iodinereached 98.86 percent.]]>
<![CDATA[INDUKSIVARIASI SOMAKLONAL JARAK PAGAR (JATROPHA CURCAS L.) UNTUK MENDAPATKAN KARAKTERTOLERAN CEKAMAN KEKERINGAN ]]>
<![CDATA[Maftuchah .]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[Experimental activities is conduct in several stages, that is : somaclonal variation induction incastor oil in vitro culture through the use of PEG and selected plantlet is reproduce in vitro. In vitroselection procedure is done by planting cotyledon explants from castor oil seedlings age 10-14 daysin nutrient medium MS+BAP+IBA. Seed sterilization is done using Tween 20, Benlate, 90% alcohol,50% chloroc and sterile water.After callus is grown, callus will be sub-cultured into nutrient mediumMS+BAP with PEG addition. Selected callus will be transferred into nutrient medium MS + potassiumpantotenat + active coal until it shows root growth.Result of experiment has shown that the higher PEG concentration will suppress thegrowth of castor oil callus. In PEG concentration 15% the mortality rate of castor oil callus (LD) is64,29% and for PEG concentration 20% the mortality rate of castor oil callus is 92,86%. For thenext phase is in vitro selection process through treatment of 15% PEG toward all seven accessions.Result of this experiment has revealed that average mortality rate of callus in 15% PEG treatment is87,64%. HS-49 has shown the lowest percentage rate of response (79%) in 15% PEG treatment,while the highest percentage rate of response occurs for SP-8 (93%). Callus that succeeding growthfrom PEG selection medium will be reproduce in regeneration medium until bud is formed. Buddingconstruct percentage is range between 28,57% (accession HS-49 and SP-38) until 37,03% (accessionSM-35). Average budding construct percentage from selected callus in regeneration medium is31,45%. For the next stage of experiment (second year), those bud will be sub-cultured into rootingmedium until forming root and become complete plantlet ready to acclimatize.]]>
<![CDATA[AKTIVITAS ANTIMIKROBA EKSTRAK LIDAH BUAYA (ALOE BARBADENSIS MILLER) TERHADAP BAKTERI PATOGEN PADA IKAN ]]>
<![CDATA[Sri Dwi Hastuti]]>
<![CDATA[ Jurnal Gamma Vol. 6 No. 1 (2010): September ]]>
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<![CDATA[“Streptocciacis” disease caused by streptococcus agalactiac has been attacked many tilapiafish farming, with the symptoms including abdominal bloating; bleeding in the eye, gill cover andbase of tail; become darker in color, and fast swmming fish unpalatable. Antibiotcs have been usedto over come this diseases, but the use of antibiotics continuously and unwisely can lead to bacterialresistence and impact on the envirotment, therefore alternative method for controlling the diseaseis important. this study was animed study was to detemine the antimicrobial activity of Aloebarbadensisi extract (saponin) toward S.agalactiae. Fresh Aloe barbandensis leaf washed thensterilized by using alcohol, dired in a preheated oven, then percolated and maserated by using nhexane and methanol for 24 hours. Then performed gravity column chromatography with a BEAdeveloper (Benzene Ethyl Acetate,Acetic acid with the ratio 75: 24: 1) on silica gel plates to ontainsaponin, then dried with a vacumm freeze dryer by using a dextrin as carrier agent, to from powder.MIC test and Inhibition zone test were performed to find the antimicrobial activity of sopaninextracted from Aloe barbadensis Miller, againt S.agalactiae. The result showed that the higher theconcentration of saponin extracted from Aloe barbadensis Miller, the better the atimicrobial activityagaint S.agalactiane. The highest of inhition zone found at concentration 12,5% with a doameterzone of 8.97 mm.]]>
