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ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) Binawati Ginting; Ilham Maulana; Nurdin Saidi; Syarifah Yanti Astryna
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Isolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive control. Isolation of the active components of TCE by column chromatography using silica gel absorbent 60 mesh ASTM (Merck 774) and the eluent n-hexane: ethyl acetate (9:1), obtained 7 fraction combined (TCE 1 to TCE 7). There are 6 combined fractions having the potential as antioxidants, namely TCE 2 to TCE 7 with a range of IC50 (6,46 ppm – 91,8 ppm). TCE 2 fraction has a very strong antioxidant activity with IC50 = 6,46 ppm. Separation of TCE 2 fraction on silica column chromatography obtained 4 combined fractions (TCE 2.1 to TCE 2.4). The test results of antioxidant activity showed that TCE 2.4 had very strong activity with IC50 = 42,7 ppm.For the TCE 2.2 fraction, preparative TLC was carried out using eluent n-hexane: ethyl acetate (9.5: 0,5) obtained by TCE 2.2.4 isolate with a melting point of 114-120 °C and was a steroid class.

PREPARATION OF CHITOSAN-CELLULOSE COMPOSITE BEADS USING CELLULOSE ISOLATED FROM SUGARCANE BAGASSE Rahmi Rahmi; Alfahmizar Alfahmizar; Lelifajri Lelifajri
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Chitosan-cellulose composite beads had been prepared using cellulose isolated from sugarcane bagasse. Sugarcane bagasse was hydrolyzed with H2SO4 with various concentrations (30, 40, and 50%). Cellulose obtained was characterized using Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Scanning Electron Microscope (SEM). FTIR analysis results inform the existence of typical functional groups of cellulose in the sample. XRD patterns show H2SO4 concentration on the hydrolysis process influence the crystallinity of cellulose. Cellulose was used as a filler in the preparation of chitosan-cellulose composite beads. The results show that cellulose decrease the crystallinity of chitosan.

USE OF PLANTS AS TRADITIONAL MEDICINE IN SWAMEDICATION IN PIDIE COMMUNITIES Hardiana Hardiana; Saida Rasnovi; Zumaidar Zumaidar
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Self-medication is an act of self-healing using plants as traditional and modern medicine without the intervention of professional medical personnel. Availability of natural materials and affordable prices encourage people to return to using traditional medicines. The purpose of this study is to find out the types of plants used by the community Pidie, plant parts and reasons for using plants as traditional medicine in self-medication. The type of research used is non-experimental with the survey method and PRA (Participatory Rural Appraisal), by interviewing 1280 respondents. Parameters of this study are the types of plants used, plant parts and reasons for using plants as traditional medicine in self-medication. The results showed that there were 38 tribes and 53 types of plants. The most widely used plant parts were leaves (44.3%), the least were roots (0.7%), and the reason for using plants as traditional medicine in self-medication was easily obtained (53.8%).

THE EFFECT OF SOME OF COCONUT WATER CONCENTRATION IN ARTIFICIAL MEDIA TO CHRYSANTHEMUM GROWTH (Dendranthema grandiflora) BY IN VITRO Nurur Rahmy; Zairin Thomy; Yunita Yunita; Essy Harnelly
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Artificial medium for in vitro culture is an alternative media to subtitute Murashige and Skoog (MS). Growmore added with organic substances as coconut water can be used as artificial medium. Coconut water can affect plant growth, so the research is to investigate the influence of coconut water in different concentration to chrysanth’s (Dendranthema grandiflora) growth should be done. The research was conducted in Laboratory of Molecular and Cell Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Darussalam, Banda Aceh, from April until July 2013. The research was designed by using Randomized Block Design with sub sampling. Media act as group, while coconut water concentration as treatment. Observation was started from growing time up to a month after planting. The result showed that there was no significant different between using MS and artificial medium with different coconut water concentration to initiate shootlet.

FORMULATION DOSAGE FORM OF TABLET CONTAINING KEPEL LEAF EXTRACT (Stelechocarpus burahol (Blume) Hook.f & Thomson) AS AN ANTIMICROBIAL AGENTS Hilda Maysarah; Meutia Faradilla; Tedy Kurniawan Bakri; Misrahanum Misrahanum
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Pharmaceutical dosage form that contains herbal materials has been developed in order to make it easy to consume. Tablet is one of pharmaceutical dosage form that generally used. A formulation and antimicrobial test of tablet containing extract of Kepel leaf with different concentration of diluents, binders, and disintegrants has been studied. The evaluation test showed that formulation consist of 25 % of Kepel leaf extract, 64.5 % of avicel PH 102, 2 % of PVP, 7 % of amprotab, 0.5 % of aerosil, and 1 % of talcum are better than the others. The experimental method used in this study was wet granulation. From the result of the mass granulation evaluation, it was obtain that speed flow without vibration was 2.53 second, with vibration was 2.83 second, bulk density was 0.329 g/mL, taped density was 0.376 g/mL and compressibility was 12.65 %. The result of tablet evaluation obtained that the average weight was 498 mg, with the average hardness was 4 kg/cm2 and disintegration time was 2.36 minutes. Antimicrobial test showed that in the concentration of 50 mg/mL, had average inhibitory diameter against S. aureus of 12.96 mm, P. aeruginosa 12.7 mm, and against B. subtilis was 12.53 mm. Whereas, concentration of 62.5 mg/mL had average inhibitory diameter against S. aureus was 13.5 mm, P. aeruginosa was 13.56 mm, and against B. subtilis was 13.43 mm. Both concentrations did not have antimicrobial activity against E.coli.

FORMULATION DOSAGE FORM OF TABLET CONTAINING KEPEL LEAF EXTRACT (Stelechocarpus burahol (Blume) Hook.f & Thomson) AS AN ANTIMICROBIAL AGENTS Hilda Maysarah; Meutia Faradilla; Tedy Kurniawan Bakri; Misrahanum Misrahanum
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.12151

Pharmaceutical dosage form that contains herbal materials has been developed in order to make it easy to consume. Tablet is one of pharmaceutical dosage form that generally used. A formulation and antimicrobial test of tablet containing extract of Kepel leaf with different concentration of diluents, binders, and disintegrants has been studied. The evaluation test showed that formulation consist of 25 % of Kepel leaf extract, 64.5 % of avicel PH 102, 2 % of PVP, 7 % of amprotab, 0.5 % of aerosil, and 1 % of talcum are better than the others. The experimental method used in this study was wet granulation. From the result of the mass granulation evaluation, it was obtain that speed flow without vibration was 2.53 second, with vibration was 2.83 second, bulk density was 0.329 g/mL, taped density was 0.376 g/mL and compressibility was 12.65 %. The result of tablet evaluation obtained that the average weight was 498 mg, with the average hardness was 4 kg/cm2 and disintegration time was 2.36 minutes. Antimicrobial test showed that in the concentration of 50 mg/mL, had average inhibitory diameter against S. aureus of 12.96 mm, P. aeruginosa 12.7 mm, and against B. subtilis was 12.53 mm. Whereas, concentration of 62.5 mg/mL had average inhibitory diameter against S. aureus was 13.5 mm, P. aeruginosa was 13.56 mm, and against B. subtilis was 13.43 mm. Both concentrations did not have antimicrobial activity against E.coli.

ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) Binawati Ginting; Ilham Maulana; Nurdin Saidi; Syarifah Yanti Astryna
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.12568

Isolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive control. Isolation of the active components of TCE by column chromatography using silica gel absorbent 60 mesh ASTM (Merck 774) and the eluent n-hexane: ethyl acetate (9:1), obtained 7 fraction combined (TCE 1 to TCE 7). There are 6 combined fractions having the potential as antioxidants, namely TCE 2 to TCE 7 with a range of IC50 (6,46 ppm – 91,8 ppm). TCE 2 fraction has a very strong antioxidant activity with IC50 = 6,46 ppm. Separation of TCE 2 fraction on silica column chromatography obtained 4 combined fractions (TCE 2.1 to TCE 2.4). The test results of antioxidant activity showed that TCE 2.4 had very strong activity with IC50 = 42,7 ppm.For the TCE 2.2 fraction, preparative TLC was carried out using eluent n-hexane: ethyl acetate (9.5: 0,5) obtained by TCE 2.2.4 isolate with a melting point of 114-120 °C and was a steroid class.

PREPARATION OF CHITOSAN-CELLULOSE COMPOSITE BEADS USING CELLULOSE ISOLATED FROM SUGARCANE BAGASSE Rahmi Rahmi; Alfahmizar Alfahmizar; Lelifajri Lelifajri
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.12734

Chitosan-cellulose composite beads had been prepared using cellulose isolated from sugarcane bagasse. Sugarcane bagasse was hydrolyzed with H2SO4 with various concentrations (30, 40, and 50%). Cellulose obtained was characterized using Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Scanning Electron Microscope (SEM). FTIR analysis results inform the existence of typical functional groups of cellulose in the sample. XRD patterns show H2SO4 concentration on the hydrolysis process influence the crystallinity of cellulose. Cellulose was used as a filler in the preparation of chitosan-cellulose composite beads. The results show that cellulose decrease the crystallinity of chitosan.

USE OF PLANTS AS TRADITIONAL MEDICINE IN SWAMEDICATION IN PIDIE COMMUNITIES Hardiana Hardiana; Saida Rasnovi; Zumaidar Zumaidar
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.13192

Self-medication is an act of self-healing using plants as traditional and modern medicine without the intervention of professional medical personnel. Availability of natural materials and affordable prices encourage people to return to using traditional medicines. The purpose of this study is to find out the types of plants used by the community Pidie, plant parts and reasons for using plants as traditional medicine in self-medication. The type of research used is non-experimental with the survey method and PRA (Participatory Rural Appraisal), by interviewing 1280 respondents. Parameters of this study are the types of plants used, plant parts and reasons for using plants as traditional medicine in self-medication. The results showed that there were 38 tribes and 53 types of plants. The most widely used plant parts were leaves (44.3%), the least were roots (0.7%), and the reason for using plants as traditional medicine in self-medication was easily obtained (53.8%).

THE EFFECT OF SOME OF COCONUT WATER CONCENTRATION IN ARTIFICIAL MEDIA TO CHRYSANTHEMUM GROWTH (Dendranthema grandiflora) BY IN VITRO Nurur Rahmy; Zairin Thomy; Yunita Yunita; Essy Harnelly
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.11541

Artificial medium for in vitro culture is an alternative media to subtitute Murashige and Skoog (MS). Growmore added with organic substances as coconut water can be used as artificial medium. Coconut water can affect plant growth, so the research is to investigate the influence of coconut water in different concentration to chrysanth’s (Dendranthema grandiflora) growth should be done. The research was conducted in Laboratory of Molecular and Cell Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Darussalam, Banda Aceh, from April until July 2013. The research was designed by using Randomized Block Design with sub sampling. Media act as group, while coconut water concentration as treatment. Observation was started from growing time up to a month after planting. The result showed that there was no significant different between using MS and artificial medium with different coconut water concentration to initiate shootlet.

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Suhartono

Contact Email
suhartono@usk.ac.id

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Journal Mail Official
jurnal.natural@fmipa.unsyiah.ac.id

Editorial Address
Block A 2nd Floor FMIPA USK Jl. Tegk. Syech Abdurrauf No. 3, Banda Aceh, 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA

Abstract

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Abstract

Abstract

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Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Suhartono

Contact Email suhartono@usk.ac.id

Phone -

Journal Mail Official jurnal.natural@fmipa.unsyiah.ac.id

Editorial Address Block A 2nd Floor FMIPA USK Jl. Tegk. Syech Abdurrauf No. 3, Banda Aceh, 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Suhartono

Contact Email
suhartono@usk.ac.id

Phone
-

Journal Mail Official
jurnal.natural@fmipa.unsyiah.ac.id

Editorial Address
Block A 2nd Floor FMIPA USK Jl. Tegk. Syech Abdurrauf No. 3, Banda Aceh, 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA