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I Wayan Sudarsa
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INDONESIA
Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 1,092 Documents
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Using of Chlorella Vulgaris for Livestock Wastewater Treatment and the Expression of NR Gene Nasrin Moshtaghi
Journal of Global Pharma Technology .
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Abstract

Algal treatment was performed for 30 days in three autoclaved livestock wastewaters. Nitrate concentrations in the three batch cultures and autoclaved livestock wastewaters were determined by spectrophotometer method. It was found that the capacity of Chlorella vulgaris removal was reached at rates of 89.22% nitrate in cattle livestock wastewater, 89.34% in sheep livestock wastewater and 89.41% in poultry livestock wastewater. Also, we studied the growth characteristics of C. vulgaris under three wastewater treatments within thirty days. The results showed that in the lag phase, the microalgae was treated in the wastewater with 501.33, 406.66, 381 mg. l-1 nitrate with a biomass productivity of   0.1, 0.09, 0.03g/l perday. On the other hand, we studied the removal of nitrite from wastewater by C. vulgaris. The results showed that the capacity of Chlorella vulgaris for nitrite removal reaches 87.50%, 43%, 65.15%, respectively. Moreover, the expression of nitrate reductase (NR)was analysed over six days in BG11 medium and livestock wastewater treatment. The highest fold of transcription was observed 96 hours after treatment in both of wastewater and culture medium. The transcript of gene at 96 hours in cattle wastewater was higher than BG11 culture medium. These result showed high potentials of Chlorella vulgaris to gene expression related to uptake of toxic nutrient and suitable candidate for gene isolation.Keyword: Algae, Livestock waste water, Nitrate reductase, Transcript.
Study the Effect of Ribavirin Drug on the histological structure of the testes in Albino mice (Mus musculus) Thekra Atta Ibrahim
Journal of Global Pharma Technology Volume 12 Issue 02 (2020) Feb. 2020
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Abstract

The results have gotten from this study was showed that all animals of two experiment groups that inoculated with Ribavirin drug appeared change in the thickness of seminiferous tubule wall of the testes and contraction in them where its appearance became irregularly and crooked as well as observed emergence of Vaculation. Also some sections of the sperm tubules Seminiferous tubule revealed increased in the widening of the distance between the germ cells and ecdysis of the epithelial tissue and aggregation in the cavity of some Seminiferous tubule also the occurrence of degeneration and raised the distance between neighboring Sertoli cells.Keywords: Testes, Sertoli cells, Ribavirin, Seminiferous tubules, Leydig cells, Mus musculus.
Psychological Tension in Some Individual Games for Students of the Third Stage in the Faculty of Physical Education and Sports Science - University of Al-Qadisiyah Mohamed Abdulrahman Mohamed
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Abstract

Today, the progress in physical education and mathematical science is clear and tangible in all sports activities, leading to higher achievement through the training process, and the introduction of all sciences to raise the level of skill performance. These sciences have a direct impact on the students' results; play an important role in achieving the desired objectives. They affect the level of students and may contribute to the implementation of technical and planning duties. Psychological stress is one of the most important psychological factors, which can be observed through the behavior of the individual in his responses to the new sports situations, in order to discourage the skillful performance of the lesson. Fencing, wrestling and gymnastics are one of the most popular games. These students from the possibilities of the skillful performance of what has been taught and these games differ from other sports in the size of sports skills, which is increasing with increasing importance, especially in the academic stages. The importance of research is to study the psychological tension among the students of the third stage in the Faculty of Physical Education and Sports Sciences who practice (dueling, wrestling and gymnastics) and compare them according to the game to know any game with a high degree of psychological tension. Through the poll of experts and specialists and the fact that researchers teaching at the college noticed, it appeared that the psychological preparation processes did not take the integrated dimension of the teachers and staff memory in the field of training, since these operations did not take sufficient quota as well as physical and skill preparation for the purpose of psychological tension control student who It reflected on the student's performance level. And to know the level of our students in the performance of the basic skills in (fencing, wrestling and gymnastics).Teachers do not treat the psychological side well during the training and the exam. It is necessary to study the side of psychological stress in the students in the third school stage to know the best material in this aspect. And compared the psychological stress of the three games in the third phase. The sample included (80) students from the Faculty of Physical Education and Sports Sciences / University of Al-Qadisiyah, which included three subjects, distributed (20) students for fencing material (20) ), A student from the Gymnasium for the academic year (2017 - 2018). The researchers used the statistical file (SPSS), which does not find the statistical analysis of the research. The researchers concluded that the students of wrestling and gymnastics subjects have the lowest proportion of students of fencing with psychological stress and recommended that students take into account the psychological stress of students in the performance of practical examinations.Keywords: Psychological tension, Individual games, Physical Education and Sports Science.
Synthesis, Thermal and Electrochemical Properties of Four New Bis Oxadiazole Copolymers Based on Azo Monomer Juman A. Naser
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Four new copolymers were synthesized from reaction of bis acid monomer 3-((4-carboxyphenyl)diazenyl)-5-chloro-2-hydroxybenzoic acid with five diacidhydrazide in presence of poly phosphoric acid. The resulted monomers and copolymers have been characterized by FT-IR, 1H-NMR, 13C-NMR spectroscopy as well as EIMs technique. The number averages of molecular weights of the copolymers are between 4822 and 9144, and their polydispersity indexes are between 1.02 and 2.15. All the copolymers show good thermal stability with the temperatures higher than 305.86 °C when losing 10% weight under nitrogen. The cyclic voltammetry (CV) measurement and the electrochemical band gaps (Eg) of these copolymers are found below 2.00 ev.Keywords: Oxadiazole, Copolymer, Azo, Monomer, Thermal Properties, Electrical Properties.
Correlation between Central Vena Pressure Measurement with Collapsibility Index of Internal Jugular Veins in Critically ill Patients Tjokorda Gde Agung Senapathi
Journal of Global Pharma Technology Volume 11 Issue 08 (2019) Aug. 2019
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Abstract

Background: Central venous pressure (CVP) is popular for assessing fluid estimation, especially critically ill patients. Another method in assessing the CVP today is by using ultrasonic cardiac output monitors (USCOM) using transaortic or transpulmonary Doppler’s ultrasound. The objective of this study was to determine the correlation between CVP and IJV collapsibility index to monitor the adequacy of the intravascular volume status in critically ill patients in ICU. Patients and Method: This analytic, cross-sectional study involved 70 patients of 19 to 64 years. The internal jugular vein (IJV) collapsibility index was examined with ultrasound cardiac output monitoring (USCOM) to assess the patient's intravascular volume status, then the data was compared to CVP. The Spearman rank correlation was used to test the hypothesis. Results: The subjects consisted of 38 males and 32 females, with an age range of 19-64 years. The Spearman’s rank correlation test showed a statistically negative correlation between the CVP value and the internal jugular venous pressure index collapsibility value (R=-0.844, p <0.001). Conclusion: There is a strong negative correlation between IJV collapsibility index and CVP in patients treated in the ICU.Keywords: Hemodynamics, Volume status, CVP, USCOM, Collapsibility index.
Pharmacokinetic Modification of Controlled Release Tablets of Antihypertensive Drug Palanisamy Vishnu
Journal of Global Pharma Technology Volume 10 Issue 12.
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Abstract

The objective of the study was to pharmacokinetic modification of controlled release tablets  of Isradipine tablets designed to provide 24 hours drug release profile using varying proportion of Eudragit L – 100 and HPMC Phthalate; Lactose monohydrate as matrix-forming material. The prepared matrix tablets showed satisfactory physicochemical properties where drug content was 99.19 % to 103.78 %, thickness was 3.23 mm to 3.36 mm, hardness was 3.37±0.13 to 3.51±0.20 kg/cm2 , friability was less than 1% and % weight variation was within the standard pharmacopoeial limits of ±10% of the weight. Mathematical analysis of the release kinetics of the optimized formulation (F15) was best fitted in zero order kinetics (R2 = 0.982). The dissolution profiles of formulation FI4 and innovator product in multi media were compared in Phosphate buffer pH 6.8.Several kinetic models respectively. The stability data reveals that the FI4 showed a negligible change in drug content thickness, weight variation, hardness, friability and in vitro drug release for three months according to ICH guidelines.
Virulence Factors of Uropathogenic Escherichia coli in Diabetic Patients of Al-Muthanna Province Elaf Lateef Neamah Al-Abbas
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Background and aims: There are evidences that patients with diabetes have an increased risk of asymptomatic bacteriuria and urinary tract infections (UTIs). UTIs are the most common bacterial infection in diabetic patients. The present study aimed to describe the characteristics of E. coli of Al-Muthanna province patients with urinary tract infection by identifying the virulence genes (FimH, Pap and HlyA). Material and methods: The study population included 240 diabetic and non-diabetic patients with UTIs as well the healthy control, Urine and blood sample was collected at Al Hussein Educational Hospital, between October 2017 to July 2018. Serum sample were collected and stored for immunological tests. Standard protocol was followed to isolate and identify organism and the data were analyzed using Microsoft Excel 2013. Results: Of the total 240, The results  of the study  were  41 (33.3%)  isolates  of  Diabetes  patients (DM group), 42(34.1%) isolates of Non-Diabetes  patients (ND group) with  urinary pathogenic  E. coli (UPEC)  and selected 40 (32.5%) of control group.  The age  duration of  46-61  years  were  most prevalence  of  UPEC  infections either  DM group  (56.1%) or  ND group (35.7%)  while  the gender  factor was shown significantly in DM and ND females (78.1% and 97.6% respectively ) compare to  males.  Twenty two  (53.7%) of diabetes urban  community  were  found to have UPEC infections while the rural infected  population were 19 (46.3%),  Non-diabetes  group  were recoded  17 (40.5%) , 25 (59.5%) positive cases of UPEC in urban  and rural  communities respectively. Fimbriae  severity virulence  genes of  DNA (fimH , pap) and  alpha-hemolysis  gene (HlyA)  of  were  extracted  of  83  UPEC isolates,  it was  then  identified  using the PCR amplification technique. The results  of molecular  diagnosis  shown that fimH was  detected  in  78%  in DM  patients  had  higher  than of  ND  patients (57%) of UPEC strains while the  percent of  Pap and HlyA were (31.7%, 12.2%) in DM patients compare to  ND patients  (Pap /38.1%)  and  (HlyA /7.1%)  of  UPEC  strains  respectively. Conclusion: UTIs are frequent in diabetic patients. For the reason of the great proportion of asymptomatic forms among diabetic patients, the urine culture should be performed in all hospitalized patients with diabetes.
Effect of Vitamin D3 Supplementations on FEV1 and FVC in Patients with Non-small cell Lung Cancer Dhaferah Jaafar Abd
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Abstract

The current study was conducted at the Middle Euphrates Center for tumors from December 2015 to July 2016 on 90 subjects, thirty healthy person were saved as a control group (G1), and the other group with 60 patients with lung cancer (LC) were subdivided into two subgroup (30 / group), group (G2 was not administered and group G3 was administered) with 1000 IU of vitamin D3) cholicalciferol). In the base line of study, vitamin D concentration and some of lung functions like Forced Vital Capacity (FVC), and Forced Expiratory Volume in the First second (FEV1) were measured for all groups. Then group G3 orally administered 1000 IU of  vitamin D3  for 63 days  then  groups of patients (G2 and G3) were exposed intravenous to 3 doses of Cisplatine 75mg/m2 and Gemcitabin  1gm/m2 for 63day (1dose/ 21 day) , then vitamin D and FVC and FEV1were measured. The results of this study showed a significant decrease (P ˂0 .01) in the level of vitamin D (8.6 ± 2.95 and 7.99 ± 3.08) ng / ml in the two groups of patients at the beginning study, vitamin D increased significantly (P ˂ 0.01) to 18.4 ± 5.8 ng / ml in group administrated with vitamin D when compared to the vitamin D in the beginning of study in the same group and group which was not administered with vitamin D.  The values of both FVC and FEV1 maintained their level close to the control values in the vitamin D administrated group (G3). It was being concluded that vitamin D 3 improved lung functions.
Association of Chicken PMEL17 Gene Polymorphism with Coat Colour Trait in Iraqinative Chicken Mostafa Sadeghi
Journal of Global Pharma Technology .
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Abstract

PMEL17 gene encodes a transmembrane protein called pre-melanosomal protein 17 or PMEL17. PMEL17 is responsible for the production of eumelanin and is present in the melanosome. The aim of this study was to investigate the genetic diversity of the partial coding regions of the PMEL17 gene and its association with coat colour in Iraqinative chicken.Furthermore, the possible impact of amino acid substitutions on effected the mutations in physicochemical properties and structure of the PMEL17 protein was investigated. Blood samples from 95 Iraqinative chickens were collected to extract DNA and the 594-bp fragment of the PMEL17 gene was amplified and then DNA sequencing method was adopted for single nucleotide polymorphism (SNP) discovering and genotyping. Sequence analysis showed that a 9 bp deletion polymorphism (GGTGCCCAG/-) at chicken PMEL17 gene. The allele frequency of this mutation is 0.84 and 0.16 for the wild allele and the mutant allele, respectively.This mutation created an early codon stop in the PMEL17 protein. The isoelectric point, net charge and hydrophobic index have significant changes by this deletion polymorphism.The association analysis showed that no significant differences between different genotypes and coat colour (p> 0.05).Key words: PMEL17 gene, Chicken, DNA sequencing, Deletion, Protein physicochemical.
Effect of Using the Innovative Light Zone to Develop the Accuracy of Performance the Forehand and Backhand Skills for Female in Tennis Mohammed Naji Shaker Abugneam
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Abstract

The researchers designed an electric-powered appliance with different light-colored signs. These signs are shown on a wooden base. Exercises were used to develop motor precision and to perform forehand and backhand in tennis. The playing area was divided into several zones, and according to the direction of each skill, the tag was used. Thus, students were able to perform two skills accurately. The game of tennis requires visual kinetic compatibility between the eye, arm, eye and foot compatibility, a visual marker designed to make students more focused and accurate on performance in difficult areas of skills. The exercises were carried out by the experimental group for 12 weeks with two training units with 24 training modules. Arteries in the variables of the statistical results showed experimental superiority over control.Keywords: Tennis exercises, Light zone appliance, Motor accuracy, Forehand and backhand in tennis.

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