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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 73 Documents
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Search results for , issue "Volume 10 Issue 06: (2018) June 2018" : 73 Documents clear
Conventional Culture Media for the Growth of Microbes Using of Date Palm (Phoenixdactylifera) Pollen Grains (DPPG) Zahraa Abd-Alrzaq
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Abstract

Background: Culture media used in the laboratory for the cultivation of microorganism supply the nutrients required for the growth and maintenance. Objectives: The study was conducted to developing alternative media namely DPPG1, DPPG2and DPPG3 by using a cheap locally available materials such as date palm pollen grains (DPPG) instead of high cost artificial media such as NA, NB,SDA and SDB For culturing some bacteria and yeast, for bacteria the test organisms used were Methicillin resistant Staphylococcus aureus (MRSA), Streptococcus fecalis, Listeria monocytogenes, Bacillus subtilis, Escherichia coli, Enterobacterae rogense, Proteus mirabilis, pseudomonas fluorescence, Acinetobacter baumanni, Klebssiella pneumonia and Salmonella sp.For yeast the test organisms used were Candida tropical is, Candida albicans, and Candida Kruzi. Materials and methods: The growth rate of microbe’s strains in liquid media was investigated by measure the optical density at 600nm but the growth on solid media was indicated by the presence of microbial colonies. The viability of the bacteria and yeast was investigated by culturing every day after the preliminary period of incubation (24 hrs.) Results: The results showed that all bacteria grew in liquid media. Listeria monocytogenes, Escherichia coli, grew equal in both DPPG1 (0.83, 0.82) and NB (0.86, 0.82). Enterobacterae rogense and Pseudomonas fluorescence grew in DPPG1 (0.88, 1.12) and DPPG2 (0.81, 0.94) higher than in NB (0.59, 1.08), Klebssiella pneumonia showed good growth in both DPPG1andNB (0.91, 0.96).Candida albicans, and Candida Kruzi showed high growth in DPPG3 (1.16, 1.10).  The most tested organisms showed good growth on the solid alternative media, and still alive in 15 selected days in liquid media and showed noticeable growth on the solid media for selected 10 daysexcept the yeast C. tropical is that stops their growth on the seventh day on SDA. Discussion: The good growth indicates that DPPG extract possess sufficient amounts of nutrients for support the growth of microorganisms. Conclusion: The DPPG extract can be use efficaciously and economically for the cultivation of the bacteria and yeasts in place of high cost artificial media which used in the laboratories.Keywords: Culture media, Date palm pollen grains, Alternative nutrient sources, NA, NB, SDA, SDB, yeast, bacteria.
Study of Feeding Preference and the Effect of Some Hosts of Aphid on Some Biological Aspect of the Coccinella Septempunctata (Coleoptera: Coccinellidae) Esraa Fadel Wathah
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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The current study included a food preference test for C.septempunctata in a modified McDonald's way inpreferring a type of aphid than the other, as well as to know the effect of the host species of prey inattracting predators. In which the type of aphid had been studied and they are Schizaphisgraminum,Aphis Nerii and thrioaphistrifolii. The results show that adult predators are attracted towards S.graminum followed by A. nerii and T. trifolii. When the adults were exposed to plants without aphid,they showed an attraction towards the Triticumspelta, followed by Nerium oleander and Medicago sativaplants successively. The study also included the testing of aphid species at different stages of life for thementioned type. The results of the study showed that the growth rate of predator larvae differedaccording to the insect host. The longest duration of the larval stage was (16091) day when feeding on S.graminum and the shortest (12.01) day when feeding on T. trifolii. As for the pupae role, the longestduration was (5.85) day when feeding on A.nerii insect. As for adults, the duration of pre-laying eggs, thenumber of eggs, incubation and the proportion of hatching differed depending on the host insect. Thehighest pre-egg laying time was (7.95) day when feeding on Nerium oleander aphid and the least (4.77)day in feeding on T. trifolii and the highest rate of eggs lying was (800.30) and the lowest (386.00) infeeding on S. graminum. The highest egg incubation period reached (4.00) day in feeding on A. Nerii andthe lowest (2.00) day in feeding on T. trifolii. The highest percentage of egg hatch is (88.82) in feeding onS. graminum and the lowest is (60.33) % in feeding on A. neriiKeywords: Hosts, Biological aspect, Coccinella Septempunctata.
Evaluation the Effect of Treatment on Melatonin Levels among Male with Nephrolithiasis Omar Khalid Suhail
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Abstract

Kidney stone formation is the source of the common disease nephrolithiasis. Oxidative stress has been increasingly linked to the high incidence of this disease. The hormone melatonin (MLT) is a biogenic amine, plays a role in the human body as an antioxidant and anti-inflammatory parameter; also it could be effective in preventing cell damage. In this study fifty-five male with nephrolithiasis with age and gender-matched (30) apparently, healthy individuals were involved. Twenty0-two of the patients treated with Tamsulosin hydrochloride and potassium citrate treatments were also comprised. Determination of sera and urine contents of urea, creatinine, electrolytes, and MLT levels were carried out. The urea and creatinine concentrations reveal significant increases in sera of patients in comparison to that of control.  A decrease in serum MLT concentration was observed in patients groups in comparison to those of control, while a highly significant diminution in urine MLT concentration was indicated in patients groups after treatment in comparison with that of other groups. As a conclusion the MLT  concentrations were altered in nephrolithiasis patients in comparison with its concentrations in both patients after treatment and healthy subjects, that proved the important role of  MLT as an antioxidant and anti-inflammatory parameter in nephrolithiasis; as well increasing MLT dietary intake or on supplementation was recommend. Keywords: Treatment, Melatonin Levels, Nephrolithiasis.
Synthesis, Characterization and Biological Activities of New Azo Dyes -Azomethine Compound and Their Metal Complexes Rehab Al-Hassani
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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The new ligand (2-thioxo-5-((4-(1-(p-tolylimino) ethyl) phenyl) diazenyl) dihydropyrimidine-4, 6(1H, 5H)-Dione (R1) have been synthesized and characterized by micro elemental analysis, Mass, (1H-13C) NMR, FT-IR, and UV-Vis spectroscopic techniques. (R1) acts as a ligand coordinating with some metal ions Co(II), Ni(II), Cu(II), Au(III) and Pt(IV) generated fife mononuclear metal complexes, represented as [CoR1Cl2(H2O)2] (a) , [NiR1Cl2] (b) , [CuR1Cl2] (c), [Pt(R1)2Cl2]Cl2(d) , [Au(R1)2]Cl3(e) .The Structures of the new compounds were characterized by elemental , FT-IR  and UV-Vis Spectra. The magnetic properties and electrical conductivities of metal complexes were also determined. The antibacterial activity for the (R1) and its metal complexes were studied against two types of pathogenic bacteria (Escherichia coli, and Staphylococcus aureus) Furthermore, the antifungal activity against two fungi) Candida albicans, and Aspergillusflavus (was studied for (R1) and its metal complexes. The anticancer activities of the ligand and their metal complexes have been extensively studied on The La cancer cell line.
The Impact of Effective Teaching Strategies in a Variety of Physical Variables and Some Basic Skills for Students in Volleyball Thuraya Jwaid Mohin
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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The common method of teaching the various skills in general and the ball in particular is the American method, which requires the teacher to be a decision maker to the student and not a slander of us on this method and reduce its advantages, but the present era is the era of knowledge and educational development should be accompanied by this Evolution Previous studies have confirmed that there are many teaching methods that are unique in each of the objectives and characteristics and have an effective role in correcting mistakes and investing time and velocity in the process of learning, but these methods meet neglect of most workers in the teaching of physical education, As well as that there are limited possibilities in the performance of sports and linking them to the basic skills of the game volleyball. So the researcher looked at this problem by introducing effective teaching with different strategies in some physical variables and the basic skills of students in the volleyball and output outputs and effective results of the educational process.Preparing educational units using effective teaching in a variety of strategies (technical, cooperative and differentiated learning) in some physical variables and basic skills of students in volleyball.Learn about the effect of using effective teaching in a variety of strategies in some physical variables and basic skills of students in volleyball.Identify which strategies are more influential in some physical variables and basic skills of students with volleyball.The researcher used the experimental method and identified the research community with the students of the second stage in the Faculty of Physical Education and Sports Sciences at the University of Babylon for the academic year 2016 - 2017, the total number of (95) students, consisting of four divisions where the random method was adopted in the selection of the sample (80) (A) The first experimental group, the second experimental group (B), the third experimental group (D), and the control group (C). The most important conclusions are:The results showed the effectiveness of effective teaching in the strategy of technical learning in the learning and development of physical variables (the strength characteristic of the velocity of the arms, the strength characteristic of the velocity of the legs, the velocity of transition).The results showed the effectiveness of effective teaching in the strategy of cooperative learning in learning and improving the physical ability of explosive power.The results showed the effectiveness of effective teaching with a different learning strategy in learning and developing physical variables in the third degree.Keywords: Teaching strategies, Physical variables and volleyball.
The Hydrogen and Sulfur Surfaces Effect on the Structural and Electronic Properties of Graphene Nano Ribbon Huda Bukheet Hassan
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Present work deals with the effect of hydrogen and sulfur surfaces on the structural and electronic properties of grapheme nano-ribbon using the B3LYP/DFT with 6-31G basis sets .Employed at the Gaussian 09 package of programs. The results showed suitable basis functions used to describe each GNR gave good relaxation and structural properties. The total energy was decreased when increasing the number of atoms in the structure. The LUMOs are greater than HOMOs in which these GNRs have the ability to donating or accepting electrons .The presence of the surfaces in the GNR increases the energy gap of the NR. For all structures, the IP is larger than the EA, means these GNRs need small energy to acceptance an electron and becomes anions. All GNRs have high electronic softness.Keywords: HOMO, LUMO, B3LYP, Softness and Hardness.
Phenotypic and Genotypic Study of Sodium Azide Effect on Proteus Mirabilis Swimming and Swarming Phenomenon Luma Abd Alhadee
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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The present study intended to detect the effect of sodium azide on P. mirabilis swimming and swarming phenomenon. Twenty one isolates of Proteus bacteria were collected from different clinical and animals samples for the period from October 2017 to November 2017. All isolates were identification depending on microscopic characterization , biochemical tests and Vitek 2E  compact system, it appears that eighteen  isolates were belong to P. mirabilis . All P. mirabilis isolates were able to swim and swarm over semi-solid media. The addition of sodium azide with (0.001, 0.01, 0.1) % concentrations was able to inhibit swimming and swarming phenomenon. Using PCR technique, WosA a gene responsible of swarming regulation was detected in all isolates and its expression was measured using RT-PCR in normal and sodium azide conditions. The result of molecular detection of WosA gene showed that all of the P. mirabilis isolates were possessed this gene and the levels of fold change of wosA gene were 0.19 and 0.12 for swimmer and swarmer cell respectively. The levels of fold change of wosA gene were 0.4 for the concentration (0.001%) of sodium azide when compared with control 1 fold change.Keywords: Proteus mirabilis, Swimming, Swarming, Sodium azide.
Preservation of Lingzhi (Ganoderma Lucidum) White Fresh Fruit Nguyen Phuoc Minh
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Lingzhi (Ganoderma lucidum) has highly valued nutritional properties. Its potential beneficial effects on human health may be termed functional foods. The production of fresh Ganoderma lucidum is increasing. This trend is expected to continue in the coming years. Fruit boby of Ganoderma lucidum has a short shelf-life owing to its high rate of respiration and perishibility. In order to extend the fresh appearance or shelf-life of Ganoderma lucidum white fresh fruit bodies, we investigated on the preservation of Ganoderma lucidum fresh fruit body. Our results showed that washing Ganoderma lucidum white fresh fruit bodies with 0.15% peroxyacetic acid in 30 seconds and keeping them in PA bag without hole in 4 o C could maintain their weight and eliminate coliforms effectively.Keywords: Ganoderma lucidum, Lingzhi, shelf-life, Peroxyacetic acid, Coliforms, Preservation
Genetic Detection for Staphylococcus aureus bacterialin Urinary Tract Infection cases in Hilla City Ali Kadhim Aljarah
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Fourteen urine Specimens were collected of patient with Different Ages & gender that had been infected with the UTI that previously Diagnosed in Hilla Educational Hospital in period from Feb. 2016 to Mar. 2016 for detection of Bacterial causative agent that cause the UTI , samples were transferred directly to laboratory then centrifuged in ( 3000 rpm ) for 15 min., taking pellet for bacterial culturing on mannitol salt agar in 37C° for 24 hr. in aerobic conditions changing the media to yellow color as an indicator for growth of S.aureusthen followed by DNA isolation from bacteria for further genetic detection by PCR, selecting ( mec A1 gene ) as an indicator gene that related to the virulence factors in the S.aureus, agarose gel electrophoresis for  mec A1 primer amplification showed 32 positive result indicated to the presence of this bacteria as one of most causative bacterial pathogenic agent in UTI, these rapid definitive diagnosis is useful for further treatment by selection the determined antibiotic drug latterly.Keywords: Mec A gene, Staphylococcus aureus, UTI, PCR
The Possible Role of JC Polyomavirus after Kidney Transplantation Haidar A Shamran
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Background John Cunningham virus (JCV) is one of the highly sero-prevalent viruses worldwide, and after primary infection it remains latent and then reactivates in immunocompromised patients, high JCV viruria seen in renal transplant recipients (RTR), some of them progress to polyomavirus associated nephropathy (PVAN). The objectives of this study are to determine JCV viruria and viremia in RTR and correlate them with urine cytology results and patients' renal function. Methods a prospective study conducted from October 2015 to to March 2016. Seventy-one (71) RTR, and 71 apparently healthy age and sex-matched normal donors as controls were enrolled in this study. Urine samples taken from all RTR cases and control, while plasma was taken from only 50 RTRs and 50 controls. Urine cytology smears were prepared and Pap-stained for decoy cells (DC) screening. Viral DNA was extracted from 1 ml urine, and from 200µl for plasma samples, and then the viral DNA was detected and measured by a real time-PCR instrument, using q RT-PCR kits for JC virus. Results Thirty one (43.70 %) of RTR and 10% of the controls were JC viruria positive, the mean of JCV viruria was 6.8 x104, and 1.04x103 copies/ml for RTRs and controls respectively (p<0.001), and 19/33 (57.58) of RTRs who had abnormal creatinine clearance were JC viruria positive (p=0.025). All 50 RTRs and the 20 controls had negative JC viremia. The rate of occurrence of DC in urine cytology was 28.17% for RTRs while none of the control subjects had DC in urine, but no correlation between JC viruria and the rate of decoy cell shedding in RTRs. Conclusion High prevalence of JCV viruria in RTRs, and associated with impairment of their renal function, which might suggest a pathological role of this virus have impact on renal function.Keywords:  JC Polyomavirus, Kidney transplantation, Real-time PCR.

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