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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol 15, No 1" : 10 Documents clear
INTRA-UTERIN ADMINISTRATION OF PROSTAGLANDIN FOR INDUCTION IN CROSS BREEDING GOATS Sri Gustari; Asmarani Kusumawati; Slamet Subagyo; Prabowo P Putro
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8622

Abstract

Productivity and reproductivity of cross breeding goats is low. It is mainly caused by hormone deficiency and difficulties in estrus detection. The research studied the effect of intrauterine administration of prostagiandin on estrus induction and on number of pregnancies.Sixteen does were divided randomly into 2 groups. Group i were injected with prostagiandin intramuscularly. Group li were given prostagiandin by intrauterine administration. In both groups, prostagiandin were given twice with interval 11 days apart. The does which coming into estrus were mated naturally.There was a significantly different in the time of estrus between group I and group II (40.25 ± 4.94 and 80.8 ± 18.35 hours). On the other hand, there was no difference in estrus induction between those two groups (Group I : 87.5% and Group II : 62.5 %). All the does which were mated naturally were pregnant (100%). Although time to estrus after intrauterine administration of prostagfandin was longer than that of intramuscular ones, but it was a cheaper method.
A STUDY OF PREPATENT PERIODES, DEATH TIMES AND REDUCTION OF BODY WEIGHT IN EXPERIMENTAL RATS (RATTUS NORVEGICUS) INFECTED WITH TRYPANOSOMA EVANSI Sumartono Sumartono; Eryl Sri Rohayati
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8623

Abstract

The prepatent periods of T. evansi, death times and reduction of foody weights of experimental rats (Rattus norvegicus) infected with T. evansi were studied.Twelve rats were divided into four groups (group 1-!V), each group contains of three rats. The rate of the group II, group III and group IV, were infected intraperitonealy with 300, 600 and 2400 T. evansi per rat, respectively. The group I was used as a control group. After infection, their body weight were measured, followed by blood examination and detection of death time everyday.The data were collected then analysed descriptively using regression-correlation between total number of T. evansi wbich were infected, prepatent period, death time and body weight changes.The results of the experiment indicated that the higher dose of T. evansi infected, caused shorter prepatent period and death time, but there were a negative correlation between total number of T. evansi infected and percentage of body weight reduction. The evidence also showed even thought the prepatent period was shorter, all experimental rats died within 5-6 days after T. evansi detected in the peripheral blood.
COLIBACILLOSIS IN BURAS CHICKENS IN SOUTH KALIMANTAN (KOLIBASILOSIS PADA AYAM BURAS DI KALIMANTAN SELATAN) Bambang Nganji Utomo
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8624

Abstract

Thirty eight buras chickens of various ages with clinical signs diseases of illness were diagnosed for colibacillosis and other diseases, The diagnoses were carried out by isolation and identification of causative agents, pathological and histopathological examination. The isolated E. coli were tested serologically and their sensitivity towards antibiotics and sulfouamid.The results indicated that E. coli were isolated from 42.11 % chickens, either as single infection or in the connection with Newcastle Disease, salmonellosis, coccidiosis, aspergillosis, pasteurellosis aad mycoplasmosis. Serological test indicated that 25% of E. coli isolates belong to serotype 0,2 : KI and 18.75% serotype 078 : K80. The Sensitivity test showed that 73.33% of E. coli isolates were resistant to the antibiotics and sulfonamid, where as 26.67% were sensitive.
RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS R. Wasito; Hastari Wuryastuti
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8625

Abstract

Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopathology diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to localize rabies virus antigen in formalin- fixed tissues. The results of the present study also demonstrated that a procedures using formalin-fixed parafin-embeded tissue and stained immunopathologicalH by the ABC method was an excellent method for both preservation of morfological details of ceils/tissues and demonstration of rabies virus antigen.
RADIO RECEPTOR ASSAY FOR DIAGNOSTIC SERUM AND MILK 1,25-DIHYDROXYCHOLECALCIFEROL Hastari Wuryastuti
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8626

Abstract

The objectives of this study were to modify and develop technique for determination of 1,25-(OH)2D3 concentrations in sera and milk samples. The developed technique, used non-HPLC for extraction and purification of the samples and involved radio receptor assay which used calf thymus as 1,25-{OH)2Dj receptor for quantitation of 1,25-(OH)2D3 Results of this study were compare to that of the technique developed by Horst et at. (1981) which used HPLC for samples extraction and purification. The correlation between the two techniques was r=0,95. Using the modified technique the 1,25-(OH)2D3 concentrations in cow and sow sera were 57,42 ±5,3 pg/ml (n=20) and 75.22 ±6.05 pg/ml (n=20) respectively, whereas the laSHOH^D., concentrations in cow and sow milk were 16.62 ±2.2 pg/ml (n=20) and 21.52 + 3.125 pg/ml (n=20). Based on the results could be concluded that the technique that had been developed in this study was found satisfactory for determination the l,25-(OH)2Dj concentration either in serum or milk samples.
DYNAMICS OF NUMBER OF NEMATODE LARVAE IN GRASS IN THE PASTURE Joko Prastowo; Sumartono Sumartono
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8641

Abstract

The existence of nematode larvae in a pasture can be used as an indication of infection or reinfection in animal grazing on it. The objective of the current study was to reveal the dynamics of the number of nematode larvae found on the grass of a pasture.Three locations of the pasture of the teaching farm of Faculty of Veterinary Medicine GMU, were chosen as sampling areas. Grass of the areas was cut just on top of the soO five times a day for ten day namely at 5, 6-9 am - 1 pm, 2-5 pm and 6 pm. Following cutting, the grass was kef t m a tube and washed with detergent solution. The washing solution was examined under a stereomicroscope, and the larvae observed were counted. Differences in the number of larvae between locations and collection times were analysed statistically by names of factorial analysis of variance.The results show that the mean number of larvae varies between 03 ± 0.67 and 6.1 ± 2.95 per grass. The number of larvae is not significantly different (F > 0,05). Cutting at however, is influenced by collection time (P < 0,05). Cutting at 5-9 am gives the largest number of larvae, while 6 pm gives the lowest count.  '
THE EFFECT OF CADMIUM CHLORIDE TO THE STERILITY OF MALE RAT Syarifuddin Tato; Puspa Wikan Sari
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8642

Abstract

The effect of cadmium chloride (CdO2) to the sterility of male rat have been observed to the first generation (Fj).Sixty white rats consisted of 30 male rats at 3 weeks old divided Into 6 groups and were given CdCl2 orally ad libitum for 42 days, Tl»e concentration of CdCl2 for each group was 0 ppin (group I), 200 ppm (group H), 100 ppm (group ID), 50 ppm (group IV), 25 ppm (group V) and 12,5 ppm (group VI). After treatments, aii male rats were mated with female rats by mixing a couple of rats in a cage for 7 days. Then male rats were kiiied all to examine the histopathoiogy of their testis. The female rats were kept until 18 days of pregnancy, then all were killed. The percentage of pregnancy and the number of baby in each female rat were counted. The histopamoiogy of seminiferous tubules of testis were analysed qualitatively.The results showed that CdCl2 at 12,5 and 25 ppm caused 20% and 40% of infertility. 50 ppm of CdCfe caused 100% of infertility and necrosis of spermatocyte and spermaride celk; there no spematozoa in the seminiferous tubules.
FIELD EVALUATION OF NEWCASTLE DISEASE VACCINE OF VG\GA IN STRAIN SEVERAL COMMERCIAL BROILER FARMS IN JAVA Charles Rangga Tabbu
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8643

Abstract

This field evaluation was conducted to study the level of protection, post vaccinal reactions and zootechninal performance of groups of chickens which have been vaccinated with Newcastle disease (ND) vaccine of VG/GA strain.This evaluation was done in 26.000 broilers from 4 farms, which have different condition in management practices and were located in different areas of Java.Chickens were vaccinated with VGAGA vaccine or with B-l/La Sota vaccine with/without inactivated ND. Serological tests were done at the age of 28 and 35 days, whereas challenge test with local ND virus isolates was done at the age of 45 days. Results of field evaluation indicated that in broilers, the VG/GA vaccine induced a high level of protection agamst the challenge test with a local (Indonesia) ND virus isolate and against infection with field ND virus. Statistical analysis indicated that the hemagglutination inhibition (HI) titers against NDV were significantly higher (P < 0,05) in group of chickens vaccinated with VG/GA with/without inactivated ND compared to group of chickens vaccinated with B-l/La Sota with/without inactivated ND vaccine. In certain conditions, the post vaccinal reactions were milder and the zootechnkal performance was better in groups of chickens vaccinated with VG/GA strain compared to groups vaccinated with B-l/La Sota.
ARTIFICIAL INFECTION STUDY OF FASCIOLA GIGANTICA IN LOCAL SHEEP AND ITS IMPACT ON WEIGHT GAIN, HEMATOLOGY AND ENZYME GAMMA GLUTAMYL TRANSFERASE Khajadatun Khajadatun; Wardiarto DP; Setyawan Budiharta
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8644

Abstract

This research was to investigate the effect of infection of Fasciola gigantica on weight gain, blood picture and the level of iiver damage of local sheep, and the correlation between the number of metacercariae infectH and the number of the fluke recovered.                                                                           Twenty male local sheep, were allocated randomly into 4 different groups. One group used as uninfected control and a total of 200, 500 and 1.000 metacercariae was infected into animals of groups 2, 3 and 4, respectively. Variables evaluated include weight gain, blood picture, gamma glutamyl tons/erase (GGTase) activity, and the number of egg and the fluke recovered. All individual sheep were weighed every week, whole blood and sera were taken every 3 weeks, fecal samples were started to b« examined 8 weeks after infection and every week thereafter. Weight gain, hematology picture and enzyme GGTase activity were anafized by split-plot, the number of egg and fluke recovered were analized by correlation. All sheep were killed 18 weeks after infection.                          The results show that infection F. gigantica causes norntocytic normochromic anemia in local sheep. Increased GGTase acitivity is observed. The number of fluke recovered correlates with the number of metacercariae infected.
CROSS-SECTIONAL STUDY ON CAPRINE SARCOPTIC SCABIES IN THE DISTRIC OF Kl LONPROGO Samkhan Samkhan; Setyawan Budiharta; Wardiarto Darjoprawito; Bambang Sumiarto
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8645

Abstract

A cross-sectional study of scabies was performed on 2615 goats from 492 small holder farmers, covered aC subdistricts and 40 out of 88 vfllages in Kulanprogo district, Yogyakarta Province. The Objectives of this study is to investigate (a) factors associated with the prevalence of the disease in goat, and (b) the prevalence uf thedisease among goat population in the district A combination of a three stage and duster sampling procedures was used to select the goat. An animal was considered as suffering from scabies if it showed cuaical sign of scabies and positive laboratory finding of Sarcoptes scabieLThe mportant findings of this study include significant association between sarcoptic scabies in the farm and cleanliness of the barn, animal density, new mcoming animal, new annual from outside of the village, intensive barn, separation of younger animal, between barn distance and closed barn. Other factos, Le. altitude, former's formal education and experience of goat raising are not significantly associated with the disease. In the animal level, new incoming animal, clinical signs, previous infestation and age are found to be associated with the occurrence of the disease. No significant association is found between the occurrence of sarcoptic scabies and sex and breed. The prevalence of the disease is found o be 93% (467492) and 73% (205/2615) for farm and animal level, i respectively.

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