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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 13 Documents

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Search results for , issue "Vol 33, No 1 (2015): JUNI" : 13 Documents clear

Infestation of Luke Worm (Fasciola sp) and Stomach Worm (Paramphistomum sp) on Adult Bali Cattle in Tenayan Raya Pekanbaru Municipality Fahrur Rozi; Jully Handoko; Rahmi Febriyanti
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted at the Laboratory of Parasitology, Veterinary Investigation Center Regional II,Bukittinggi in January until February 2013. Bovine fecal samples of Bali cattle were taken in the District of Tenayan Raya Pekanbaru Municipality. This study was aimed to determine the prevalence of paramphistomiasis and fascioliasis of adult males and females Bali cattle and the number of eggs worm per gram of feces as well. A total of 232 fecal samples were tested by Parfit and Banks, and McMaster. Results showed that the prevalence of Fasciola spp. in males Bali cattle and cows was 49.02% and 60.71%, respectively, whereas the prevalence of Paramphistomum spp. in males Bali cattle and cows was 46.07% and 50.00%,respectively. Result count of the average number of worm eggs using Mcmaster in the fecal samples of Bali cattle in males and females was 205.88 eggs/g and 267.85 eggs/g, respectively. This study indicated that the eggs worm counts per gram feces could be classified into mild infection.

Microscopic Agglutination Test for Diagnosis of Leptospirosis in Beef Cattle from Bantul and Kulon Progo Susanti Susanti
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Leptospirosis is a zoonotic disease caused by bacterial infection, Leptospira interrogans. The bacteria usually infect many kinds of wild animals and humans as well. There was an extraordinary outbreak of leptospirosis in humans in Yogyakarta in 2011. Therefore, it is necessary to investigate the seroprevalences of leptospirosis in cows, especially in the districts of Bantul and Kulon Progo in order to determine the possible role(s) of the cows in the spread of leptospirosis among other animals and humans. In this research, the diagnostic method was conducted by using microscopic agglutination test (MAT). Bovine sera samples were obtained from several farms in some areas of the districts of Bantul and Kulonprogo. The MAT was conducted on 150 sera samples of cows from districts of Bantul and was on 200 samples from Kulonprogo. Seroprevalences of leptospirosis in the cows were 18.67 % ( 28/150 ) and 14.5% (29/200) from Bantul and Kulonprogro districts, respectively. Mostly, serovar tarassovi, hardjo, icterohemorrhagiae and batavia were detected in cows from both Bantul and Kulonprogo districts. Antibody titers were obtained ranged from 1:100, 1:400 and 1:1600.

Levels of Blood Glucose and Total Protein of Repeat Breeding Dairy Cows From Daerah Istimewa Yogyakarta Dhasia Ramandani; Alfarisa Nururrozi
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Repeat breeding in dairy cows is a reproductive disorder characterized by a length of calving interval.This research was aimed to determine levels of blood glucose and total protein as one of the causes of repeat breeding. This research used ten repeat breeding Friesian Holstein cross breeds at the age of 3-8 years old from Daerah Istimewa Yogyakarta. They have had birth at least once, normal reproductive cycle, and in a healthy condition. Ten ml blood samples of each cow from jugular vein were collected and analyzed at LPPT UGM. Blood glucose and total protein plasm were examined using a photometer Microlab 300 with a spectrophotometer. The data ware analyzed descriptively. The results showed that dairy cows with repeatedbreeding case had lower blood glucose and total protein plasm concentrations than that of the normal. The average concentrations of blood glucose and total protein plasm were 48.58±6.675 mg/dl and 6.815±821 g/dl, respectively.

Escherichia coli Surface Characters of Ettawah Cross Breed Goats Milk on the Adhession Ability of Mammary Epithelial Cells Lalita Prasiddhanti; A.E.T.H. Wahyuni
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Escherichia coli is a bacteria that may cause mastitis in goats. The ability of bacteria to infect is influenced by surface characters of each bacterial cell. Adhesion of bacteria on the udder epithelial cells plays an important role in the incidence of subclinical mastitis.The purpose of this study was to determine the surface characters of Escherichia coli colony morphology, such as the presence of polysaccharides capsule, hemagglutination activityand hydrophobicity contributes to the adhesion process. Three isolates of Escherichia coli isolated from Ettawah cross breed goats on Mandiri and Pangestu Farm at Turi, Sleman, Yogyakarta were used in the study. The ability of E. coli to fermentize lactose was done by examining its ability in fermented lactose on eosin methylene blue. Hemagglutination ability was seen by hemagglutination test using sheep erythrocytes at concentrations of 0.5%, 1%, 1.5% and 2%, respectively. Hydrophobicity expression was done by saltaggregation test using ammonium sulfate ([NH4] 2SO4) at concentrations of 1.2 M, 1.6 M, 2.0 M, 2.4 M and 3.2 M, respectively. The presence or absence of sera proteins was tested with sera soft agar, and the ability of adhesion was done by adhesion of Escherichia coli which has been marked by fluorescein isothiocyanate , with rats udder epithelial cells after birth. The results showed that isolates of E. coli with dry surface characters had athin polysaccharide capsule, positive hemagglutination , and hydrophobic, which had the ability to stick more on the udder epithelial cells after birth. Meanwhile, those with mucoid surface characters, had a thick polysaccharide capsule, negative hemagglutination, and hydrophilic which had a lower adhesion ability.

A Biological Study of Larvae and Adult Hemonchus contortus in Goat Yuswandi Yuswandi; Rika Yuniar S
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

A biological study of larvae and adult Hemonchus contortus was carried out in goat. The aims of this study were to know the capability of the infective larvae (L3) development to adult, and the prepaten period of H. contortus. A number of 14 goat abomasum collected from Ngampilan Slaughterhouse, Yogyakarta Province was used as source of H. contortus. Eggs worm were collected from direct surgery of the H. contortus in physiological saline. The egg development of H. contortus to the L3 stadium was carried out by a modification Harada-Morito, whereas the development of the L3 to adult and the prepaten period were studied in the goat asexperimental animal in vivo. Before the goat necropsied, the diagnosis of H. contortus egg was done every two day post infection and started two weeks after infection. The data was analyzed descriptively. The results showed that the capability of the egg development of the worm to the L3 stadium was 0,33%, the capability of the L3 development to adult was 32,42%, and the prepaten period of H. contortus was 21 days.

Blue Eyes Syndrome Cases in the Duck Farms in the Village Modopuro, Mojosari, District Mojokerto, East Java Albiruni Haryo
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Modopuro village in Mojokerto, East Java is a region where people are mostly producing local breeds of ducks that is well known called as a Mojosari duck. The Modopuro village located in a lowland area where their people have been farming the Mojosari local ducks for years. It was reported that the outbreaks of the Mojosari ducks occurred during the years of 2012 and 2013. The ducks died suddently with the clinical signs, such as discoloration of the eyes (blue eyes syndrome) within 3 days, torticolis, stumbling, and they were then dead.From the many anxiety of farmers will be the disease, it is necessary to identify the causes of disease and determination of disease prevention measures. Inspection carried out by bringing the infected ducks to Laboratoritum Pathology Faculty of Veterinary Medicine, Airlangga University to do a necropsy, histopathological examination, and HA - HI examination have done in Virology Laboratory of Veterinary Medicine, Airlangga University. Histopathological examination showed the presence of lesions in organs eyes, lungs, heart, and ceca tonsil. Further, HA-HI test indicates that 90% possibility of of disease blue eyes syndromeon duck farms in the village Modopuro in the second trimester of 2013, caused by the H5N1 virus.

Isolation and Identification of Egg Drop Syndrome Virus with Hemagglutination and Hemagglutination Tests Fidyah Fitrawati; Michael Haryadi Wibowo; Surya Amanu; Bambang Sutrisno
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Egg drop syndrome (EDS) is a disease that attacks layer hens in the production phase causing failure of peak eggs production, decreased in eggs production, and presence of eggs without shell. This study was conducted to isolate and identify the EDS virus in the chicken layer that was diagnosed as a disease of EDS by hemagglutination (HA) and hemagglutination inhibition (HI) assays. Specific pathogen free (SPF) layerchickens which were passing through the production phase fed with food which was mixed with egg without shell from SR/WNO/2011. The chicken together with chicken FF/Sleman/2011 were dissected when pathological lesions, such as the dents or palor eggs observed. The uterine tissues were then collected for samples. Infundibulum of chicken FF/Sleman/2011 was explored and was found out that the eggs were lack ofegg shells. The eggs were then washed using sterile PBS. The three subsequent samples were propagated in the allantoic fluid of embryonated duck eggs for 16 days. Allantoic fluid was harvested after being incubated for 4 days. It was then tested by HA and HI assay by use of avian influenza virus (AIV), Newcastle disease virus (NDV), and EDS anti serum. The HA and HI test with EDS anti serum used chickens erythrocytes in percentageof 0,8. The HA test in uterine sample of both SR/WNO/2011 and FF/Sleman/2011 showed the titer 23 HA units and egg washed water sample of FF/Sleman/2011 showed titer 22 HA units. The HI test for comparison with ND and AI anti serum was negative, while the test with EDS anti serum showed positive results. Based on the HA and HI test results, it was concluded that the virus grown in the allantoic fluid is EDS virus.

Acute Toxicity Test of Polysaccharides Krestin from Coriolus versicolor Extract with Parameters of Hepatocyte Damages, SGPT and SGOT Enzyme in Mice Andita Ayu Mandasari; Sri Puji Astuti Wahyuningsih; Win Darmanto
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Coriolus versicolor is a mushroom that has polysaccharopeptide krestin (PSK) and polysaccharopeptide (PSP). Many reports showed that polysaccharide krestin as a compound which could reduce mutagen induction, radiation, and development of cancer spontaneusly. However, all substances entering in body could change become toxic depending on dosage. Therefore, this research was aimed to know the effects of PSK on hepatocyte damages, SGPT and SGOT enzymes. Polysaccharide krestin was given by intraperitoneal injection once with treatment groups as follow: K0, was given only saline; P1, was given 80 mg/kg BB PSK dosage; P2, was given 120 mg/ kg BB PSK dosage; P3, was given 160 mg/ kg BB PSK dosage; P4, was given 200 mg/ kg BB PSK dosage; dan P5, was given 240 mg/ kg BB PSK dosage. The results showed that PSK caused hepatic lesions, such as parenchymatous (hydrophic) degeneration of hepatocytes in groups P2 and P3, mild to moderate necrosis in group P4, and moderate to severe necrosis in group P5. Level of SGPT enzyme was not increased, butlevel of SGOT enzyme was increased in mice given 160 mg/ kg BB dosage with value 151,62 ± 26,62 IU/ L.

The Effect of Turmeric (Curcuma Domestica Val.) Extract on the Histopathological Lesions of Lung Induced by Cigarette Smokes in Wistar Rats laelatul Rahmad; Nida Ul Milah; Agapietta Kusumawardani; Novi Herliyani; Kurniati Sarwendah; Bambang Sutrisno; Hastari Wuryastuti; R Wasito
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This present study is aimed to observe and determine the effects of turmeric (Curcuma domestica Val.) extract administration towards the cytotoxicity effect of cigarette smokes in the lungs of Wistar rats. Twenty Wistar rats, male, 3 months old, were used and divided randomly into four groups, of five each. Group I is as a control (K I), Group II was given 9 cigarettes/day without turmeric extract administration (K II), Group III giventurmeric extract 1 ml 0.25%/day (K III) and Group IV was given 9 cigarettes/day and turmeric extract 0.25%/day (K IV) for 60 days. At the end of the present study, all rats were necropsied and organs, particularly the lungs were collected, processed histopatologically and stained routenely with hematoxilyn eosin. The results indicated that the lungs had macrophages infiltration in the septal alveoli and hyperplasia of bronchial associated lymphoidtissue (K II). Whereas, the lungs in the rats in Groups K I, K III and K IV were still within the normal limits. It is concluded, that turmeric extract may have a potential antipulmonary cytotoxity effect due to cigarette smoke.

Antioxidant Effect of Ethanol Extract of Bulbus Dayak Onion (Eleutherine americana) to the Quality of Spermatozoa Exposed by Cigarette Smoke in Rats (Rattus norvegicus) Demes Chornelia Martantiningtyas; Anni Nurliani; Rusmiati Rusmiati
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The toxic substance within the cigarette smoke causing oxidative stress that may damage the quality of spermatozoa. Oxidative stress occurs because the amount of reactive oxygent species and antioxidant produced by the body are not in balanced. One of the alternative approaches of natural antioxidant produced by plant is dayak onion. The bulbus of dayak onion contains steroid, tanin, saphonin, naphtoquinon and flavonoid compound. The aims of this study are to find out the ability of antioxidant from etanol extrat of dayak onion bulbus to increase the quality of spermatozoa affected by smoke and to find out the dose of etanol extrat of dayak onion bulbus that can increase the percentage of viability, morfology, and amount if spermatozoa affected by smoke. In this study, three dose of treatments: 30 mg/kg BW, 60 mg/kg BW, and 90 mg/kb BW were used. The results of dose 30 mg/kg BW, 60 mg/kg BW, and 90 mg/kb BW extended the increased in the normal morfology and amount spermatozoa percentage rather than that of only treated by only the smoke, whereas the viability fordose of 30 mg/kg BW cannot increased in the percentage of spermatozoa viability after affected by smoke.

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