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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Histopatologis Dugaan Edwardsiella tarda sebagai Penyebab Kematian Ikan Maskoki (Crassius auratus) : Postulat Koch Atik Ratnawati; Uni Purwaningsih; Kurniasih .
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

ABSTRACT The aim of the study was to know the influence experimental infection of Edwardsiella tarda was isolated from sick koi fish (Cryprinus caprio) to the histologic features of liver, kidney, gill, brain, intestine and muscle in Goldfish (Carassius auratus) based on Koch Postulate. Three goldfishes (KK) were used in this reseach. KK was infected by Edwardsiella tarda as much as 0.1ml i.m.. KK1 died on the 4th day and KK2 and KK3 died on the 25th day. All the fishes were then necropsied. The livers, kidneys, gills, brains, intestines and muscles were taken for making the histopathologic preparation. Bacteriologic observation of KK1 was conducted by cultivating the bacteria into Brillliant Green Agar (BGA), Triple Sugar Iron Agar (TSI Agar), Urea Agar, Motility Agar, Peptone Broth, sugars and oksidation test. Result of histopathologic observation showed that there was necrosis, congestion and macrofag limpocytes and neutrophisl infiltrations in the livers,colloid mass and melanomacrofages in the kidney, congestion and proliferation of secondary lamellae, limfosit and macrofag infiltration of the brain, a melanomacrofag of the muscle and the intestine showed there was limphocytes, and macrofages infiltration in the brain, and melanomacrofages in the muscles as well. It was suspected that those bacteria was Edwardsiella tarda.

Study of The Effects of Carboxymethyl Chitosan on The Non-specific Defense System in The Carp (Cyprinus Carpio) Ristyana Dewi Hernawati; Triyanto .; Murwantoko .
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Carp (Cyprinus carpio) is a freshwater fish with a high economic value, but very susceptible to diseases. One of effort to increase the productivity is by enhancing non-specific defense system. The purpose of this study is to determine the effect of carboxymethyl chitosan on enhancement non-specific defense system of carp. Carboxymethyl chitosan was obtained by alkylation process in which monochloroacetic acid in alkaline conditions was added. Carboxymethyl chitosan was given to carps at dosages of 30 μg/g, 75 μg/g and 105 μg/g, by intra muscular injection respectively. Seven and 14 days after administration of carboxymethyl chitosan, measurements of non-specific immune system parameters were done. The results showed that, administration of carboxymethyl chitosan on carps affected the phagocytic activity and lymphocytes counts. However, carboxymethyl chitosan did not give any effect to NBT activity, hematocrit, number of erythtocytes and leukocytes, monocytes and neutrophil counts in blood as well.

The Effectiviness of Ketapang (Terminalia cattapa L.) Leave Extract for the Treatment of Aeromonas salmonicida Infection in Catfish (Pangasioniodon hypophthalmus) Sumino .; Asep Supriyadi; Wardiyanto .
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Catfish is one of the fish species with certain advantages that attract the farmers interest to culture. The species becomes popular following the successful spawning in the pond. Additionally, catfishes have high economic value due to their rapid growth and response to good artificial feed. Their farming activities are inseparable from concerns about their potential diseases. Diseases in farmed fish can be caused by fungi, parasites, bacteria and viruses. One type of bacterium to take serious control is Aeromonas salmonicida. This pathogenic bacteria may cause furuncle and death of farmed fish. The use of ketapang leaves is effective in inhibiting or killing the pathogenic A. salmonicida in catfish. In the present study, each test used a sample of 10 catfishes of approximately 10 cm in size. Parameters of the test consist of survival rates and pathologic lesions. The feed treatment consisted of ketapang leaf extract in concentrations of 100, 200, and 300 mg / ml, and a positive control and a negative control. Each treatment consisted of 3 replicates, and were analyzed statistically. The results indicated that the LD50 (Lethal Dose), MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) are 107 cfu/ml, 50 mg/ml and 100 mg/ml, respectively. The most effective concentration of ketapang leaf for the treatment of A. salmonicida infection in the catfish is 200 mg/ml. This is indicated by the highest level of catfish survival and the lowest rate of the pathologic lesions.

Cytotoxic Tobacco Smoke in The Cornea of Wistar Rats Fed Turmeric (Curcuma Domestica Val.) Extract Agapietta Kusumawardani; Kurniati Sarwendah; Laelatul Rahmad; Nida Ul Milah; Novi Herliyani; Bambang Sutrisno; Hastari Wuryastuty; R Wasito
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract An increasing number of smokers who tend to rise rapidly from year to year has become a major focus of public health issues. Exposure to environmental tobacco smoke, containing toxic chemicals can trigger acute or chronic inflammation and oxidative stress in the eyes. To overcome this condition, phytotherapeutic and/or phytopreventive approaches which are efficatious, safe and cheap is needed. One of spices from Indonesia which is greatly possible to be promoted as a herbal medicine, is a phytochemical curcumin in turmeric. This present study was conducted to identify and determine the effects of turmeric extract (Curcuma domestica Val.) as phytotherapeutic and/or phytopreventive in the eyes lesion of Wistar rats induced by cigarette smokes. Twenty male Wistar rats at 3 months old were divided randomly into four groups of five each. Those were the control group (KI), the group given 9 cigarettes smoke/day (K II), the group given turmeric extract 1 ml 0, 25% /day (K III) and the group given 9 cigarettes smoke/day and 0, 25% turmeric extract 1 ml /day (K IV) for 60 days. Water and feed was given ad libitum. At the end of the experiment, all rats were euthanized with ether, necropsied andeyes balls were collected, fixed formalin 10% and stained with routine hematoxylin-eosin. The results of the present study indicated that 9 cigarettes smoke/day increased mitotic activity and caused hyperplasia in the corneal epithelial cells (K II), and addition of turmeric extract 0, 25% 1 ml per oral for 60 days prevented both mitotic activity and hyperplasia in the corneal epithelial cells (K IV). Extract of turmeric (Curcuma domestica Val.) has been proved to have herbal therapeutic and/or preventive effects againts the pathotoxicological cigarette smokes. Further study needs to be done to more evaluate pathogenesis of the eyes lesions and phytotherapeutic and/or phytopreventive effects of turmeric extract as well.

Reproductive Organ of Male Sulawesi Giant Rat (Paruromys dominator) Ni Luh Putu Rischa Phadmacanty; R. Taufiq Purna Nugraha; Wirdateti .
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Indonesia has a biodiversity in fauna and one of them is the rat. The methodology to identify animal character is mostly based on morphological structure, the example of body lenght and hair color. However, reproductive organs of every species are diferent in location, shape, color and weight. Paruromys dominator is one of rats species in Sulawesi. Although, many informations about the rat's morphology is already provided , but no data about reproductive organ reported yet. Knowing, identifying and understanding the reproductive organs in that species must be done by sampling reproductive organs and measure all of them,. All of the reproductive organs were then collected for anatomical sample and processed for histological staining and were analyzed with light microscope and SEM. Based on the present study, Paruromys dominator has reproductive organs which are like other murids, namely seminal vesicle, testis, prostat glands, Cowper's glans and glans penis. There is no diference about histological stucture of rat's testis with other rats species, but based on the result of SEM, the penis has many penile spines. The sperm head is hook shape, it is like Rattus norvegicus. The average of head length and tail lenght of sperm are 1,28±0,101 μm and 12,95±1,139 μm, respectively.

Responses of Bones, Kidneys and Parathyroid Glands in Wistar Rats Fed Containing Variation of Phosphor Levels Hartiningsih .; Sutjipto Nitisuwirjo; Hastari Wuryastuty
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract The objective of the research was to study the responses of bones, kidneys and parathyroid glands of calcium homeostasis in the Wistar rats fed containing variation of phosphor levels. Twenty female Wistar rats at 40 days of age were randomly divided into four groups (A, B, C and D) of five each. The group A was fed diet containing Ca:P ratio = 1.5:1 (diet I/control). The ratio of calcium and phosphorus was 1.5:3 (diet II), 1.5:6 (diet III) and 1.5:9 (diet IV) and were given to the rats in groups B, C, and D, respectively. Each of the rats was placed into individual cages. All of rats were fed diet for 12 weeks and water was provided ad libitum. At the end of the study, blood was collected from the heart for calcium and phosphor analysis, while parathyroid glands, kidneys and left femurs were collected and routinely stained with hematoxylin and eosin for histopathological examination. In the present study, the diet containing Ca:P = 1.5:6 caused decrease of blood calcium, whereas the diet containing Ca:P = 1.5:9 caused decrease of blood calcium and increase of blood phosphorus. Histopathological examination of the parathyroid glands of the rats in group A showed normal structure. The parathyroid glands of the rats in groups B, C and D showed vacuoles in principle cells of the parathyroid glands. The increase of the vacuoles in the principle cells of the parathyroid glands was higher than that of rats in groups B, C and D, respectively. Histopathological examination of kidneys of rats in group A showed normal structure, whereas kidneys of rats in groups B and C and D showed metastatic calcification of renale tubules. The kidneys of rats in groups C and D showed metastatic calcification of the inner and outer layers of tubule lumen, atrophy and necrosis in the epithelial cells of renal tubules, fibroblasts proliferation in interstitial cells, and infiltration of mesangial cells. The kidneys of rats in group D showed proliferation in the epithelial cells of the glomeruli as well. Histopathological examination of metaphysis of proximal femurs of rats in group A showed normal structure, whereas metaphysis of proximal femurs of the rats in groups B, C and D had osteoclasts infiltration, and fibroblasts were predominantly seen in the bone cortex. The increase of osteoclasts in the bone cortex in rats in groups B, C and D was higher than that of rats in group A. The fibroblasts proliferation in the bone cortex was higher in rats in groups C and D. Based on the results of the present study, it was concluded that the diet containing Ca:P = 1.5:3 caused decrease of blood calcium and . The diet containing Ca:P = 1.5:6 caused decrease of blood calcium, hyperplasia of parathyroid glands, , acute nephrosis, and fibrous osteodystrophy. The diet containing Ca:P = 1.5:9 caused decrease of lood calcium and increased of blood phosphorus, hyperplasia of the parathyroid glands, chronic glomerulonephritis and fibrous osteodystrophy.

Hypertropic Osteopathy (Marie Diseases) in The German Shepherd Dog Dhirgo Adji
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.3507

Abstract A Male German Shepherd dog, five years old, twenty six kg of body weight was submitted to Professor Soeparwi Animal Hospital, Faculty of Veterinary Medicine, Gadjah Mada University by Mr. Siswo Han, the owner who lives in Cangkringan, Yogyakarta on May 13, 2013. The dog was suffered from the pain and lameness on the front-left extremity for about 2 weeks. The dog having registered number of 8738/V/A/RSH/13 did not show any specific clinical signs, except the lameness. Skin and hairs, mucous membranes, lymphoid glands, and respiratory, digestive, circulatory and uropoetica organs as well were within the normal limits. The result of standard blood test for both red blood cell and white blood cell were still within normal values. Evaluation of fecal sample of the dog was also normal meaning that no parasites were found in the fecal sample. Radiographic analysis of the upper left extremity and the lungs area showed that there was an irregular bone growth on the left caput humeri, and the lungs were normal. Based on the lesion, the dog was diagnosed as having a hyperthropic osteopathy (Marie Disease).

Ovulatory Follicular Dynamics After Estrus Synchronization Using Intravaginal Progesterone Implant in Dairy Cows Prabowo Purwono Putro
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract The study aimed to follow ovulatory follicular dynamics and plasma progesterone profile after estrus synchronization using progesterone intravaginal implant CIDR and its combination with PGF2a and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were divided into 3 treatment groups. Treatment 1, CIDR implant was inserted intravaginally for 9 days (CIDR); treatment 2, given CIDR and 250 mg GnRH intramuscularly at implant removal (CIDR + GnRH); treatment 3, CIDR and 25 mg PGF2a at day 7 of implant insertion (CIDR + PGF2a). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz transducer was performed everyday for 12 days to follow ovulatory follicular dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data were tested using analysis of variance and correlation analysis. The result indicated that CIDR implant was a potent agent for estrus synchronization with the onset of estrus was 66.18 + 03.42 hours after its removal. The addition of GnRH resulted the highest ovulatory growth rate following the implant removal (1.67 + 0.17a, 1.93 + 0.13b dan 1.53 + 0.20a mm/day, P < 0.05). Insertion of CIDR hampered dominant follicular development and its removal caused rapid follicular growth and followed by ovulation. Its insertion resulted steady increase in plasma progesterone levels and its removal resulted in sudden decrease in the hormonal levels, induced ovulatory follicular development, followed by estrus and ovulation. The CIDR implant also inhibited luteal growth, caused no effect of PGF2a administration on decrease of plasma progesterone levels. Addition of GnRH at the time of CIDR removal enhanced the highest rate of ovulatory follicular growth.

Isolation, Identification and Sensitivity Test of Staphylococcus aureus against Amoxicillin of the Milk sample in the Mastitis Crossbred Ettawa Goat at Girimulyo Area, Kulon Progo, Yogyakarta Amalia Krishna Dewi
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is the main bacteria causing mastitis of the Ettawa crossbreed goat. The mastitis causes significant economic losses due to decreasing of milk production. Mastitis in goat could threat the survival rate of her kids because decrease in quality and quantity of milk products. The toxin produced by S.aureus can also cause the death of their mothers. The present research is aimed to isolate and identify S. aureus from mastitis milk sample of Ettawa crossbreed goat, and does its sensitivity to amoxicillin as well. The milk samples were collected from the mastitis crossbreed Ettawa goat at Girimulyo area, Kulonprogo, Yogyakarta. In the present study, 20 milk samples of Ettawa crossbreed goat were used to isolate and identify S. aureus based on the culture, Gram staining, biochemical and sugar tests. The isolates then tested for their sensitivity to amoxicillin. Nine isolates in the present study identified as S. aureus in which they grew on MSA, formed round cells cluster, Gram +, fermented manitol, lactose and maltose. They coagulated the rabbit plasma and reacted positively to the clumping factor and Voges Proskouer test. Based on the results of the sensitivity test to 10 ìg amoxicillin is known that 8 isolates (88,89%) are sensitive to amoxicillin. Therefore, it can be concluded that there are 9 isolates from 20 mastitis milk samples of Ettawa crossbreed goat identified as S. aureus. Staphylococcus aureus isolates are mostly sensitive to amoxicillin.

Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency Widodo Suwito; A.E.T.H. Wahyuni; Widagdo Sri Nugroho; Bambang Sumiarto; Utomo Bimo Bekti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.

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