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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Influence of Palm Sugar Water in the Native Chicken Performance Fera Aryanti; Muhammad Bayu Aji; Nugroho Budiono
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Palm sugar containing 66,18% sukrose is an additional source of energi quickly available to the chicken. A study to examine the effect of palm sugar in the native chicken performance was held in animal health training center, Cinagara-Bogor lasted from August until November 2012. This present experiment using 1274 native chicken that were kept starting DOC. Palm sugar concentrations given in the drinking water as much as one percent started to be given to the chcken when they were still DOC. After that, the palm sugar water was given continously intermittent interval by administering multivitamin. The control group was not given the palm sugar. Chicken aged 0 to 6 weeks were given palm sugar twice a day. After reaching over 6 weeks old, palm sugar water was only given onece a day. Result obtained showed that the administration of palm sugar water affected the increase in body weight gain, feed consumption and lower mortality as well. The result also showed that the FCR is lower than that of the control group until 6 weeks old.

A Life Table of Spodoptera litura Fabr. with Different Artificial Diets Sri Lestari; Trisnowati Budi Ambarningrum; Hery Pratiknyo
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Sustainable insect supply is a routine activity in research of pest control, experimental insecticide bioassay, entomophatogen, parasitoid, and nature of competitor. Armyworm (Spodoptera litura Fabr.) is one of the alternative test insect. A mass rearing method is required to provide high demands of S. litura. A life table of insect is regularly used to study several biological parameters including the net reproduction rate of population (Ro), the mean length of life period in one generation (T), reproduction potential value of one generation from a population (rm), and the potential of a population to multiply in one generation (ë). The aim of this study were to asses the development time and reproduction potential of S. litura which different of artificial diets. Results showed that S. litura on artificial diet in recipe of Singh and Moore is T = 29,90, R = 2692,33, r = 0,26, ë = 1,29, o m whereas artificial diet in recipe of Waldbauer et al. is 32,29, R = 891,99, r = 0,21, ë = 1,23. o m

Protection Capacity of Kaffir Lime (Citrus hystrix) Peel Extract Against Dengue Haemorrhagic Fever Mosquitoes Joni Hendri
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract The use of chemicals as mosquitos repellent may cause health problems. Extract of caffir lime (Citrus hystrix) as mosquitoes can be used as another option.The present study was aimed to analyze the protection capacity of C. hystrix against Aedes aegypti and Ae. albopictus. The experimental study using completely randomized design was done. The obtained data were calculated using the protection capacity formula and analyzed using t-test. The result indicated that protection capacity of Citrus hystrix extract for 6 hours on average gave 34.82% of protection against Ae. aegypti and 41,44% of Ae. albopictus.The caffir lime extract has been able to reject the mosquitoes, Ae. aegypti and Ae. albopictus. Although the thrust of the caffir lime is not as good as chemical products, butit can be used as alternative mosquitoes repellent.

Identification of Campylobacter jejuni Using Polymerase Chain Reaction Method Liza Angeliya; Ruri Rumpaka Kurdiwa
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Campylobacter jejuni is a curved-rod shaped bacteria, non-spore, microaerophilic Gram-negative and motile. It is commonly found in the feces of animals. They grow at a temperature of 37-42° C and are zoonotic causing a disease called campylobacteriosis. Campylobacter jejuni naturally presents in the chicken digestive tract and does not cause any diseases. However, chicken carcasses contaminated with this bacteria are closely related to the the incidence of campylobacteriosis in humans. Cases of human campylobacteriosis primarily caused by Campylobacter jejuni contamination on chicken carcasses. During the cutting process, Campylobacter jejuni will be able to spread to the chicken carcasses. Alternative approach for detecting Campylobacter jejuni contamination of chicken products is by using polymerase chain reaction (PCR) method. Therefore, to determine diagnosis of animal diseases in relation to the veterinary public health due to Campylobacter jejuni is by applying PCR with forward primer: 5'-TGACGCTAGTGTTGTAGGAG-3 and reverse primer: 5'-CCATCATCGCTAAGTGCAAC-3'. The PCR primers can amplify DNA Campylobacter jejuni as long as 402 bp. The PCR method is a molecular laboratory approach which is sensitive, specific, rapid and accurate for determining diagnosis of campylobacteriosis.

The Detection of Encoding Gene of Toxic Shock Syndrome Toxin-1 S. aureus Isolate from the Milk of Cows and Goats by Polymerase Chain Reaction Rony M Kunda; A.E.T.H. Wahyuni; Rini Widayanti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Staphylococcus aureus is the main bacterium found in cow's and goat's milk. The bacteria can produce toxin called toxic shock syndrome toxin-1 (TSST-1) that can infect humans and animals causing several serious diseases. The objective of this study was to detect the existence of encoding gene of TSST-1 S. aureus isolate from cow's and goat's milk. The research is initiated by re-identification stage of S. aureus conventionally continued by identification based on the molecular method of polymerase chain reaction (PCR). A number of 10 S. aureus isolates from cow's and goat's milk cultured in aerobics continued by Gram stain, catalase, coagulase, MSA, VJA and VP tests. The identification of S. aureus based on the molecular approach conducted by 16S rRNA gene amplification continued with amplification of TSST-1 encoding gene as the target gene. The PCR product of TSST-1 encoding gene then sequenced to ensure whether the DNA fragment amplified is the TSST-1 encoding gene or not. The result of the research indicates that re-identification of S. aureus conventionally generating positive reaction of S. aureus species. Molecular identification of 16S rRNA gene amplification gives a good result by producing DNA fragment of 745 bp size and meets the target gene. The detection result of encoding gene TSST-1 gives negative result marked by DNA fragment which the size does not match the target gene. The allignment result of sequence isolate SA.1 indicates that the sequence is not tst gene but the gene which coded glutamate sinthetase belongs to S. aureus, whereas sequence of isolate KI.8 is 50S rRNA gene belongs toS. saprophyticus.

Blood Profile of Wistar Rats due to Subcronic Condition Caused by Sodium Nitrite Dyah Ayu Widyastuti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Nowadays, chemicals used as food additives are increasing. One of the chemicals commonly used is sodium nitrite (NaNO2 ). Sodium nitrite is found in sausage and another meat product. Sodium nitrite is used as food preservative agent. But, the presence of NaNO2 can influence blood cells ability to carry oxygen. It causes anemia and forms nitrosamines, carcinogenic agents. This research is purposed to know blood profiles of Wistar rats on subchronic condition caused by NaNO2 . The research was done for 3 months as subchronic time. Twenty seven Wistar rats were divided into 3 groups, control group (K), first group (P1) treated by NaNO2 with dose of 11,25 mg/kg bodyweight/day, and second group (P2) treated by NaNO2 with dose of 22,50 mg/kg bodyweight/day. Blood samples were collected from orbital sinus and tested once a week for hematological parameters, including white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT), lymphocyte level, and neutrophil level. The data were analyzed with ANOVA using SPSS 16 program for Windows and the blood profiles alteration were seen. The results from this study showed there were no significant differences among the blood profiles of Wistar rats treated by NaNO2 with dose of 11,25 mg/kg 2 bodyweight/day and dose of 22,50 mg/kg bodyweight/day compared to that of the control group, except WBC values at subchronic condition at dose of 22,50 mg/kg bodyweight/day which decreased 23,46%. The NaNO 2 could not influence the blood profiles of rats treated by NaNO2 subchronically.

The Effects of Turmeric Extract (Curcuma domestica Val) on Cigarette Smoke Cardiotoxicity in The Wistar Rats Nida Ul Millah; Agapietta Kusumawardani; Laelatul Rahmad; Novi Herliyani; Kurniati Sarwendah; Bambang Sutrisno; Hastari Wuryastuty; R Wasito
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.3811

Abstract The objective of this research is to determine the effects of turmeric (Curcuma domestica Val) extract administration toward heart histopathologic lesions of male albino Wistar rats + 3 months old exposed by cigarette smoke. Twenty male Wistar rats were divided randomly into four groups of five each: Group I was given neither cigarette smoke exposure nor turmeric extract administration (control) (KI), Group II was given cigarette smoke exposure of 9 cigarettes/ day (KII), Group III was given 1 ml turmeric extract 0.25% (KIII) and Group IV was given cigarette smoke exposure of 9 cigarettes/ day and 1 ml turmeric extract 0.25% (KIV). After 2 months of treatment, necropsy was conducted to all rats and the heart samples were collected to be processed histopatologically and stained routinely with hematoxylin-eosin. The results of the present study indicated that the cigarette smoke caused cardiomyopathy in KII marked by the histopathologic lesions such as vacuolation, homogeneous eosinophilic cytoplasms, edema and necrosis of myocardia, also congestion and mild hemorrhages (KII). Whereas, rats in Groups KI, KIII and KIV had normal histologic structures of myocardia. It is concluded, that turmeric extract could be a preventive herbs toward cardiotoxic effects of cigarette smoke. Further research has to be conducted to determine the anti cardiomyopathy mechanism of turmeric rhizome due to cigarette smoke exposure.

Morphological Characterization and genetic Diversity of Macrobrachium rosenbergii (De Man, 1879) Crossbreeding Result from Samas, Bone, and Sintetis Populations Trijoko .; Niken Satuti Nur Handayani; Anggun Feranisa
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract In Indonesia, research for the prime seed of Macrobrachium rosenbergii crossbreeding is rarely done. The aims of this study are to study F1's morphological character and genetic diversity from the result of crossbreeding between M.rosenbergii Samas, Bone, and Sintetis populations. In this study, genetic characterization was known by using PCR RAPD method utilizes three primers and morphological characterization. Data were analysed with UPGMA algorithm and Simple Matching coefficient that were presented in dendrogram. F1's coefficient heterosis was counted based on ratio from the average of cephalothorax and abdoment lenght and also ratio from the average of standard length of charapax and the average of abdomen length. The results showed that there was a very high genetic diversity in F1 population. The specific locus was found in individuals from Sintetis and Samsam populations.100% Polimorfism was found from F1's DNA amplification result, meanwhile monomorphism 50% was found from parent's DNA amplification result on OPA 20. The highest F1 heterosis was found in Samas and Sintetis genotipe.

Molecular Detection of Taura Syndrome Virus Infections in White Shrimp (Litopenaeus vannamei) and Giant Prawn (Macrobrachium rosenbergii) Fariha Willisiani; Nur Rohmah; Irma Nur Rahmawati; Nastiti Wijayanti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Giant prawn (Macrobrachium rosenbergii) and vaname shrimp (Litopenaeus vannamei) are types of shrimp that became excellent commodities in the fisheries sector. However, one of the obstacles in the vaname shrimp aquaculture is a disease caused by the infection of Taura Syndrome Virus (TSV). One of the consequence of raising the vaname shrimp in Indonesia is the possibility of spreading TSV infection in another shrimp species. TSV infection in giant prawns in Indonesia has not been reported. The aims of this study were: 1. To determine the resistance of giant prawns toward TSV infection and 2. To detect molecularly using RT-PCR technique the presence of viruses TSV on vaname shrimp or giant prawns infected with 3 different doses (0.05 ml; 0.10 ml and 0.15 ml) of TSV inoculum using a pair of specific primers for TSV 9992F (5'-AAG CTT GCG TAG ACA GCC-3') and 9195R (5'-TCA AGA ATG GCT TCC TGG-3'). The research results showed that vaname shrimp mortality infected by 0.05 ml; 0.10 ml and 0.15 ml TSV inoculums were 14.28%, 42.86%, and 57.14%, respectively. Whereas the giant prawns mortality that were infected using the same dose of TSV inoculums were 0%, 8.33%, and 8.33%, respectively. Positive result was detected molecularly only from haemolymph of vaname shrimp infected using 0.15 ml of TSV inoculum. On the other hand, positive results were detected in pleopod and the gill of vaname shrimp infected using 0.05 ml; 0.10 ml or 0.15 ml of TSV inoculums. In giant prawns, infection using 3 different doses of TSV inoculums causes negative result molecularly. Based on all of the facts, it can be concluded that, giant prawns has the higher resistance to TSV infection than that of vaname shrimp.

The Effect of Decis Insecticide on the Mortality and Gill Histological Structure of the Red Indigo "Cangkringan local strain" Wahyuni Wulandari; Sukiya .; Suhandoyo .
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract This study was aimed to determine the effects of Decis insecticide on the mortality, its safe level concentration against mortality, and the cytotoxic effects in the gills of the red tilapia strains of "localCangkringan" collected from Fish Seed Center, Cangkringan, Sleman, Yogyakarta. Eight hundreds fishweighing 0.5-1.0 g and 2-12 weeks old each were collected randomly and divided into 5 groups with Decis insecticide concentration of 0.27 ppm, 0.81 ppm, 1.00 ppm, 1.35 ppm, 2.4 ppm, respectively and a control group based on preliminary testing. Each treatment comprised three replicates, 10 replicates of each test fish. The observations of fish mortality to the test were done every hour - 24, for 96 hours of treatment, whereas mortality at safe levels test done every 4 weeks for 2 months of treatment. Data were analyzed using probit analysis to determine LC50 and LC50-96 hours-48 hours. The assay of 10% safe levels of LC50-48 hours is 0.13 ppm. Twofactorial analysis of variance was to determine the effect of concentration and duration of exposure to the Decis insecticide test fish mortality. Independent samples of T test analysis was applied to determine the effect of safe levels. The descriptive and qualitative analysis was done to determine the pathologic lesions on fish gills due to the cytotoxicity of Decis insecticide. Results of the present study showed that the variation of concentrations of Decis insecticide has significant effect on fish mortality (p <0,01). Decis insecticide concentration used for the LD50(96 hours) was 1.21 ppm that was the highly toxic class to Nila. It caused severe cytotoxic efects to the gills indicated by the presence of gills necrosis, hypertrophy, hyperplasia dan diffuse hemorrhages. The safety levelsof Decis concentration still caused mild histopathological lesions, such as focal to multifocal hemorrhages and congestion in the gills

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