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Nur Indah Septriani
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INDONESIA
Berkala Ilmiah Biologi
Published by Universitas Gadjah Mada
ISSN : 08537240     EISSN : 29644429     DOI : https://doi.org/10.22146/bib.v14i3
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Biologi Fungsional, Struktur dan Perkembangan, Bioteknologi dan Biologi Molekuler, serta Biologi Lingkungan dan Biodiversitas
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol 14 No 1 (2023)" : 5 Documents clear
Effectivity of Entomopathogen Fungus Metarhizium anisopliae (Metchnikoff) Sorokin to Mortality of Aedes aegypti Linnaeus, 1762 Larvae Amanda Novitasari; Agustina Citra Windianingsih; Thiwuk Leres Kinanti; Siti Sumarmi; Sukirno; Hidayat Soesilohadi
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.4774

Abstract

The Aedes aegypti mosquito is the vector that causes the spread of the Dengue virus that causes Dengue Hemorrhagic Fever. Breaking the chain of spread of Dengue fever has been done a lot, but it can cause negative impacts to environment. It is necessary to use other alternative solutions. One of them uses natural bioinsecticides from entomopathogenic fungi. The entomopathogenic fungus that has been widely developed as a bioinsecticide is Metarhizium anisopliae. Metarhizium anisopliae is a fungus which has larvicidal activity. Isolates of Metarhizium anisopliae were obtained from the Center for Forecasting Plant Pest Organisms, Karawang, West Java and Aedes aegypti larvae were obtained from Parasitology Laboratory, Faculty of Medicine, Public Health, and Nursing, UGM. The research was conducted from November 2021-April 2022 at the Entomology Laboratory, Faculty of Biology and the Parasitology Laboratory, Faculty of Medicine, Public Health, and Nursing, UGM, Yogyakarta. The method in this research is the culture and manufacture of a suspension of the fungus Metarhizium anisopliae with an average conidia density 0,9 x 107, 0,57 x 106, 1,175 x 105, 5,25 x 104, 3 x 104, 12,5 x 103 of conidia/mL, rearing of Aedes aegypti, bioassay, and data analysis using Probit Test with SPSS software version 23 to determine LC50. The effective concentration to kill 50% of larvae was 0.9 x 107 conidia/ml. There was no significant effect between each different concentration in killing Aedes aegypti larvae. The LC50 value obtained was 1.4 x 107. Metarhizium anisopliae was effectively to control Aedes aegypti larvae.
Monitoring the Diversity of Amphibian and Reptiles In the Upstream part of Code River Using e-DNA Method Donan Satria Yudha; Rahma Izzati; Aulia Sigit Ardianto; Ananto Puradi Nainggolan; Dwi Sendi Priyono
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.4847

Abstract

Research on the diversity of amphibians and reptiles on the upstream part of Code River had been done in 2012 and 2017. Result on two previous results were found anurans, snakes and lizards but we did not find any freshwater turtles. Method used on the 2012 and 2017 research was conventional using Visual Encounter Survey (VES). Conventional methods such as VES have limitations, which was the inability to detect difficult-to-find animals such as freshwater turtles. Therefore, a new approach was taken to monitor the diversity of amphibians and reptiles especially to detect freshwater turtles in the Code River, namely the environmental DNA (eDNA) method. The purpose of this research were to monitor the diversity of amphibians and reptiles, and to reveals eDNA sampling efficiency compared to VES. An e-DNA metabarcoding process is conducted with universal CO1 primer on the water sample taken from upstream part of Code River. The results obtained were compared to the data from conventional studies held in 2012 and 2017. The results in 2021 found 12 species of amphibians but all of these species did not naturally distribute in Yogyakarta and even in Indonesia. Furthermore, 5 species of snakes were found, with only 1 species of snake naturally occur in Yogyakarta. Subsequently, we found 6 species of lizards whose natural distribution does not exist in Yogyakarta and even in Indonesia. Finally, we obtained 1 species of tortoise whose natural distribution does not exist in Yogyakarta and even in Indonesia. By far, in Indonesia the identification of amphibian and reptile species in rivers using the e-DNA method has shown less accurate results. The inaccuracy may be due to the lack of specificity of the sequence targets and primers for monitoring reptiles and amphibians in rivers. The target sequences used in this study did not have significant differences between species in reptiles and amphibians, so that the herpetofauna that had been recorded in the Code River were not identified using the e-DNA method. Based on this research, conventional sampling is recommended for monitoring herpetofauna in river areas. Keywords: Monitoring, Amphibians; Reptiles; Code River, e-DNA
Inventarisasi Kupu-Kupu (Lepidoptera: Rhopalocera) di Suaka Margasatwa Paliyan, Gunung Kidul uliya fasa
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.5241

Abstract

A wildlife sanctuary is a location where the environment is still preserved so as to minimize forest conversion activities. Roaming method or exploration is the method that the autor uses in research butterfly inventory. Exploration is carried out by following the transect which adjusts the shape of the location with a distance between points of 20 meters. Areas that become observation locations including open land, fields, forest medium mixed and dense mixed forest. Butterfy data obtained through documentation or captured using insect nets observed, then release again. The aim of this research is identify butterfly species and environmental parameters present in the field clearing, field, medium ixed forest, and dense mixed forest. Results of the study are found 22 species butterflies with a total of 65 indiviuals. Previously, research related to butterfly inventory in Wildlife Sanctuary Paliyan has never been done. The conservation of butterflies is need because of the importance of the presence of butterflies as a determinant of environmental quality. Butterfly diversity disclosure can be used as a related database types of butterflies in Indonesia, especially the Paliyan Wildlife Sanctuary.
Resistance of Three Maize Varieties to Downy Mildew Fungus Peronosclerospora maydis Infection Ristiyani Khofifa Putri; Rina Sri Kasiamdari
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.5658

Abstract

Maize (Zea mays L.) is one of the staple foodstuffs of the cereal group which has a high percentage of carbohydrates, protein, and others. In 2050, maize is expected to double in demand, however, productivity must be balanced with the support management of diseases and pests of maize. Common maize disease is Downy mildew caused by Peronosclerospora spp., this disease attacks the leaves and seeds with 100% damage. This research aimed to determine the pathogenicity and resistance of Talenta, Pioneer 21, and Tamara maize varieties, by using in vivo leaf insertion and leaf spray methods. Fungal isolates were isolated from threshing conidia from maize leaves in the Sleman area field. Observation of fungal pathogenicity and maize resistance was measured based on symptom scale per two days of test and observation of leaf anatomy 45 days after planting. Calculation of fungal pathogenicity against maize varieties used growth analysis, disease incidence, disease severity, Area Under the Disease Progress Curve (AUDPC), and severity unit per time. The result of this research showed that Talenta was moderately resistant, Pioneer-21 was resistant, and Tamara was susceptible to Downy mildew. Downy mildew pathogenicity rate reached 0,07 units/day. Downy mildew infection reached epidermis tissue through stomata on maize leaves.
Characteristics of Water Storage Capacity and Water Storage Efficiency of ‘Biotextile’ Growing Medium for Erosion Resistance Utami, Dyah Nursita; Kusumastuti, Etty; Sudiana, Nana; Rahayu, Budi; Hidayat, Nur; Sulistiawan, Insan Nur; Purnomo, Agus; Atang; Sittadewi, Euthalia Hanggari
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.5891

Abstract

Abstract: Biotextile is an innovation in erosion-resistant growing media technology, made from organic cocopeat supplemented with Legume Cover Crop (LCC) seeds and coated with biofertilizer. In its application, the success of biotextiles is determined, among other things, by the capacity and efficiency of water storage in the media The capacity and water storage efficiency of biotextile media will determine the success of growth in field applications. From field tests conducted on the sloping land of the Ciater reservoir cliffs, South Tangerang, it was shown that on the 4th day the LCC seeds had started to grow, on the 10th day they had grown in all biotextile cells, and at the age of 3 months the LCC plant canopy had covered almost all of the soil surface at the test field. Based on the growth test results, biotextile characterization tests have been carried out, which include measuring water content (KA), water storage capacity (KPA), and water storage efficiency (Es). Based on these test results, a biotextile characteristic test has been carried out which includes water content (KA), water storage capacity (KPA), and water storage efficiency (Es) to find out the characteristics of KPA and Es in evaluating its success and to prove the hypothesis that KPA and biotextile Es support the successful growth of land cover plants (LCC). The measurement of KA biotextile was carried out using the gravimetric method, while KPA and Es were measured using the immersion method, which was carried out in the Serpong laboratory. From the results of laboratory analysis, KA reached a range of values: 40–80%, KPA = 74.41–297.59%, and Es = 21%–75.5%. Apart from the results of plant growth and laboratory results, from a physical perspective, the performance of biotextiles can be seen in reducing the energy of rainwater blows, reducing the flow rate of surface water, and reducing soil erosion.

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