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INDONESIA
Indonesian Journal of Microbiology
Published by Indonesian Journal Publisher
ISSN : -     EISSN : 30479193     DOI : https://doi.org/10.47134/ijm
Core Subject : Science,
Immunology & microbiology
Indonesian Journal of Microbiology aims to serve as a premier platform for the dissemination of high-quality research and advancements in the field of microbiology. The primary focus is on original research articles, reviews, and methodological studies that contribute to our understanding of microorganisms and their diverse roles in various environments.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 15 Documents
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Sifat Fisiko-Kimia dan Fungsional Tepung Umbi Talas Belitung Metode Pengukusan dan Fermentasi Oktavia Eka Muthia Bahari; Huda Oktafa; Heri Warsito; Dina Fitriyah
Indonesian Journal of Microbiology Vol. 2 No. 4 (2026): January
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v3i1.5820

Abstract

Talas belitung merupakan umbi yang berpotensi sebagai salah satu sumber alternatif pangan lokal. Pengolahan umbi talas menjadi tepung dapat memperluas pemanfaatannya menjadi berbagai jenis produk olahan pangan. Tujuan penelitian ini yaitu mengetahui karakteristik sifat fisiko-kimia dan fungsional tepung talas. Penelitian menggunakan desain eksperimen sederhana dengan tiga perlakuan berbeda yaitu kontrol (P0), pengukusan 30 menit (P1), dan fermentasi spontan 72 jam (P2). Setiap perlakuan dilakukan pengujian sebanyak 2 kali ulangan untuk melihat perubahan karakteristik fisiko-kimia dan fungsional tepung yang dihasilkan. Hasil penelitian menunjukkan bahwa sifat fisik tepung dengan warna paling cerah (L=78,10) pada perlakuan fermentasi, sedangkan rendemen tertinggi pada kontrol (22,82%) dan densitas tertinggi pada pengukusan (0,48 g/ml). Sifat kimia pada perlakuan fermentasi memiliki beberapa nilai tertinggi dibandingkan perlakuan lain yaitu kadar protein (5,73%), lemak (1,42%), serat pangan total (4,14%), pati resisten (16,10%) dan abu (1,63%). Sedangkan kadar karbohidrat tertinggi pada kontrol (81,80%) dan kadar air terendah pada pengukusan (9,38%). Sifat fungsional pada perlakuan pengukusan memiliki nilai tertinggi dibandingkan perlakuan lain yaitu pada swelling power (360,64%) dan water holding capacity (213,75%), sedangkan nilai water holding capacity tertinggi pada perlakuan fermentasi (105,45%).  Perbedaan metode penepungan akan mempengaruhi karakteristik fisiko-kimia dan fungsional tepung yang dihasilkan. Perlakuan fermentasi spontan memiliki lebih banyak keunggulan dibandingkan perlakuan lain terutama pada sifat fisik dan kimia.
Revolutionizing Water Management in Agriculture: A Photovoltaic Solar Irrigation Model for Sustainable Citrus Cultivation Mahmood Jamal Abdulhasan; Hawraa Ahmed Mohammed; Murtadha Hasan Abed; Asawer Hasan Sharif; Baraa Jabbar Attar; Safwan Nadweh
Indonesian Journal of Microbiology Vol. 2 No. 4 (2026): January
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v2i4.5927

Abstract

Sustainable agriculture system is also based on the approach that through methods focusing on environmental conservation and resource efficiency — food and agricultural resources could suffice for the present generation and continue to be able to meet this need of future generations. The study investigates the water footprint of orange cultivation and photovoltaic solar-driven irrigation as one of the approaches to assuring energy-water security in a water-scarce region such as Latakia Governorate, Syria. Since water has become so difficult to access, and the cost of energy is still high, one of the challenges for most agricultural operators who would like to produce oranges continues to be the lack of an adequate water supply. The output implies that the orange trees in Latakia need to eat 15,000-18,000 m3/yr of water during the growing season. Compared to surface irrigation with 60% and drip irrigation which runs between 70–85% efficiency if the energy is reliable, solar irrigation systems can use as much as 90% of the water input. The annual water consumption rates are minimized to 12,000 m3/acre and equivalent surface irrigation cut back of solar irrigation systems because the common floor area in western Iran is said to be about30–40% of the hour riverine groundwater extraction sectors [4]. As there is no fuel or power drawn to the electronic grid, solar systems require low maintenance and operation costs making them an affordable solution providing a payback period of three to five years. The results of the research leads to an understanding of the citrus water requirements in Latakia, and show that solar irrigation represents an appropriate sustainable method for using these available water resources with low cost saving non-renewal sources and providing a non-dependent cultivation.
Isolasi dan Identifikasi Bakteri Probiotik pada Buah dan Sayuran Edible dari Pangandaran, Jawa Barat Marshella Syahla Khairunnisa; Ratu Safitri
Indonesian Journal of Microbiology Vol. 2 No. 4 (2026): January
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v2i4.5932

Abstract

Buah dan sayuran merupakan sumber pangan nabati yang berpotensi menjadi habitat alami Bakteri Asam Laktat (BAL) yang dapat dimanfaatkan sebagai probiotik. Namun, informasi mengenai BAL indigenous dari komoditas lokal Indonesia masih terbatas. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi awal BAL dari buah dan sayuran edible asal Pangandaran, Jawa Barat, sebagai kandidat probiotik berbasis sumber daya hayati lokal. Penelitian dilakukan secara deskriptif eksploratif menggunakan sampel pisang, sawo, pakis, dan petai. Isolasi bakteri dilakukan pada media de Man Rogosa Sharpe Agar (MRSA) dan de Man Rogosa Sharpe Broth (MRSB), kemudian dilanjutkan dengan karakterisasi fenotipik melalui pengamatan morfologi koloni, pewarnaan Gram dan spora, serta uji katalase, oksidase, dan tipe fermentasi. Hasil penelitian menunjukkan bahwa diperoleh sembilan isolat bakteri. Tujuh isolat memiliki karakteristik yang sesuai dengan kelompok BAL, yaitu Gram positif, tidak membentuk spora, katalase negatif, dan oksidase negatif. Berdasarkan karakter fenotipiknya, isolat tersebut memiliki kemiripan dengan genus Lactococcus, Lactobacillus, dan Leuconostoc, sedangkan dua isolat dari petai diduga memiliki kemiripan dengan genus Acinetobacter dan Sphaerobacter. Hasil penelitian menunjukkan bahwa buah dan sayuran asal Pangandaran berpotensi menjadi sumber BAL indigenous yang dapat dikembangkan sebagai kandidat probiotik. Namun, identitas isolat masih perlu dikonfirmasi melalui identifikasi molekuler
A Systematic Review of Diagnostic Accuracy: Serological and Molecular Tests Compared to Culture for Invasive Fungal Infections Dhuha A. Athouf; Hawraa A. Mohammed; Fatima Assad Baker
Indonesian Journal of Microbiology Vol. 3 No. 1 (2026): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v3i1.6038

Abstract

Invasive fungal infections (IFIs) remain a major clinical challenge, particularly among immunocompromised patients, due to their high morbidity, mortality, and diagnostic complexity. Despite being the gold standard, traditional fungal culture has low sensitivity and takes a long time to produce results. Recently, serological and molecular diagnostic approaches have emerged as potential alternatives to earlier and more precise IFI detection. The goal of this systematic review was to evaluate the effectiveness of various non-culture approaches and their potential impact on diagnostic precision. To detect invasive fungal infections (IFIs), this systematic review compared the diagnostic accuracy of commonly used genetic and serological approaches to fungal culture. A thorough literature search yielded eligible studies testing assays including β-D-glucan, galactomannan, cryptococcal antigen, and PCR-based approaches. A random-effects model was employed to calculate pooled sensitivity and specificity. While several serological techniques demonstrated good specificity and consistency across numerous fungal species, molecular assays were often more sensitive than culture, particularly for early detection. Despite variations in research quality and inconsistency in diagnostic thresholds, the data show that combining serological and molecular approaches significantly improves diagnosis accuracy and may reduce delays associated with culture-based identification. This systematic review demonstrates that for the diagnosis of invasive fungal infections, both molecular and serological diagnostic procedures outperform culture-based approaches. Molecular assays are important for early diagnosis due to their high sensitivity and rapid turnaround time. Serological tests are highly specific and reliable when performed correctly.
Bioinformatics Analysis of Molecular Mimicry Between Human Microbiome Antigens and Autoimmune Receptors in Multiple Sclerosis Dhuha A. Athouf
Indonesian Journal of Microbiology Vol. 3 No. 1 (2026): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v3i1.6183

Abstract

Multiple Sclerosis (MS) is a chronic autoimmune disease characterized by neuroinflammation and demyelination of the central nervous system. While genetic predispositions like the HLA-DRB1*15:01 allele are well-documented, environmental triggers—particularly gut and oral dysbiosis—are increasingly implied in initiating the aberrant immune response. Molecular mimicry, where microbial antigens share structural or sequence homology with self-antigens, is a primary mechanism hypothesized to drive this autoreactivity. This study aims to systematically screen, model, and evaluate potential molecular mimicry between the human microbiome proteome and key MS-associated neuro-autoantigens (Myelin Basic Protein [MBP], Proteolipid Protein [PLP], and Myelin Oligodendrocyte Glycoprotein [MOG]) at both sequence and 3D structural levels. An integrated in silico pipeline was developed. High-throughput sequence alignment was performed using BLASTp (optimized for short, exact matches) to cross-reference MS autoantigens against a curated database of MS-associated microbial proteomes (e.g., Akkermansia muciniphila, Bacteroides fragilis). Epitopes were filtered using the Immune Epitope Database (IEDB) to predict binding affinity to the HLA-DRB1*15:01 restriction element. Candidates with an IC50 < 500 nM were modeled in 3D using AlphaFold3/ESMFold. Structural mimicry was quantified via backbone superimposition (TM-align/PyMOL), filtering for a Root-Mean-Square Deviation (RMSD) < 2.0 Å. Finally, molecular docking simulations via AutoDock Vina/HADDOCK evaluated peptide-MHC binding kinetics and subsequent T-cell receptor (TCR) cross-activation. We expect to identify a distinct repertoire of gut and oral microbial peptides exhibiting high sequence and conformational homology to myelin epitopes. Top candidates are predicted to display robust stable binding energies within the HLA-DRB1*15:01 cleft and effectively engage MS-specific TCRs, demonstrating functional cross-reactivity. This computational framework provides a high-resolution map of microbial triggers in MS, highlighting specific therapeutic targets for microbiome-derived interventions and offering deeper insights into the environmental etiology of autoimmune demyelination.

Page 2 of 2 | Total Record : 15

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