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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 14 Documents
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Search results for , issue "Vol. 9 No. 1 (2022)" : 14 Documents clear
INHIBITORY ACTIVITY OF Trichoderma harzianum AGAINST PUTATIVELY PATHOGENIC FUNGUS ON RODENT TUBER (Typhonium flagelliforme) PLANT Catur Sriherwanto
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Trichoderma spp. are globally considered as the most dominant biofungicide in the market. Reports on Trichoderma spp. efficacy against pathogenic fungi in commercial crops have been numerous, but much less in medicinal plants. This study aimed at testing the potential biofungicidal activity of Trichoderma harzianum in inhibiting the growth of a putatively pathogenic fungus isolated from rodent tuber (Typhonium flagelliforme) plant. The methods consisted of isolation of fungi from the plant’s surface, soil, and polybags. The isolates were then screened for their putative pathogenicity against rodent tuber before being subjected to 16S rRNA molecular identification and in vitro antagonist test using T. harzianum. Result showed that only isolate K4 showed pathogenicity on T. flagelliforme, and was molecularly identified as Lasiodiplodia theobromae, known globally as fungal pathogen attacking various plants. L. theobromae was inhibited by T. harzianum with inhibition index of 23.0 ± 4.3%, which was about twice higher than that of the positive control nystatin 100.000 IU mL (11.1 ± 0.6%). In conclusion, T. harzianum inhibited the growth of L. theobromae in vitro, hence indicating its biofungicidal potential.
ETHANOL EXTRACT OF Moringa oleifera INCREASED THE NUMBER OF SPERMATOZOA AND IMPROVED SPERM MORPHOLOGY OF OLD Rattus norvegicus Luh Putu Widiastini
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Aging in men can lead to decreased semen volume, abnormal sperm morphology, and decreased sperm motility. This study aims to determine the effect of ethanol extract of moringa (Moringa oleifera) leaf on the number and morphology of spermatozoa in old Wistar rats (Rattus norvegicus). This study used healthy old rats, 18–19 months old, with a bodyweight of 200–250 g and no physical disabilities. The 36 rats were divided into 2 groups, i.e. the treatment group (fed with ethanol extract of moringa leaves 50 mg/kgBW/0.5 mL CMC 0.5% every day) and the control group (0.5 mL CMC 0.5% every day) for 30 days. The results showed that moringa leaf ethanol extract increased the number of spermatozoa (p-value 0.000) and improved spermatozoa morphology (p-value 0.000). It can be concluded that the ethanol extract of moringa leaves significantly increased the number and improved the morphology of the spermatozoa in the aged rats.
SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Mohammad Ubaidillah
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample.
THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Sri Kasmiyati
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe.
MITOCHONDRIAL DNA DIVERSITY IN FOUR POPULATIONS OF INDONESIAN FRESHWATER GIANT PRAWN (Macrobrachium rosenbergii) Ratu Siti Aliah
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Mitochondrial DNA has been widely applied to analyze fish genetic diversity, especially the cytochrome oxidase subunit I (COI) gene, which can be used as a marker of typical variation patterns, both intraspecific and interspecific crossbreeding. Haplotype diversity was observed in four populations of giant freshwater prawns (Macrobrachium rosenbergii) originating from Peureulak River in Aceh, Tabuk River in South Kalimantan, Bengawan Solo River in East Java, and the Siratu strain. Haplotype diversity was observed in the mitochondrial DNA COI gene of 1516 bp and restricted using 5 enzymes, namely AvaII, MspI, RsaI, HaeIII, and TaqI. The diversity of mtDNA COI haplotypes in the population of Peureulak River, Tabuk River, Bengawan Solo River, and Siratu were 0.8183, 0.6222, 0.7461, and 0.8044, respectively. The dendrogram of genetic distance showed that the Bengawan Solo River populations and the Siratu strain were in the same cluster, while Peureulak River and Tabuk River populations were in another group. The four populations can be used as genetic sources in crossbreeding activities based on haplotype data for each population, haplotype diversity, and genetic distance.
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Yulita Nurchayati
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ.
MOLECULAR IDENTIFICATION AND MORPHOLOGICAL CHARACTERIZATION OF PATCHOULI (Pogostemon sp.) FROM BATANG REGENCY, CENTRAL JAVA PROVINCE Hermin Pancasakti Kusumaningrum
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Patchouli (Pogostemon cablin Benth.) is an essential oil-producing plant from Batang Regency that has excellence in patchouli alcohol contents and highly survives in any condition. Molecular identification has been done in the ITS region because DNA sequences in the ITS rRNA region evolved faster than in other areas. This study aimed to get the molecular and morphological identity of patchouli (Pogostemon sp.) from BPP Kabupaten Batang. The study consisted of sample preparation, DNA isolation, amplification, electrophoresis, sequence data analysis, and phylogenetic analysis using MEGA X. All parts of plant were morphologically identified and compared with patchouli from Sidikalang, Aceh, Java, and China. Extraction DNA produced 301.7 ng ?L–1 concentration and 1.93 purity. Amplification of ITS fragment patchouli produced a 670 bp-sized single band. Phylogenetic analysis showed patchouli BPP related to Pogostemon cablin (KR608752.1) with 98% coverage identity. BPP patchouli showed 62,5% morphological similarity with Sidikalang patchouli compared to Java patchouli. In conclusion, BPP patchouli is a Sidakalang patchouli P. cablin that has undergone environmental adaptation.
GENETIC MUTATION INDUCTION OF Monstera adansonii ON VARIOUS MUTAGENS BY DRIPS APPLICATION Dwi Wahyuni Haswin
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Monstera adansonii has wide leaves with a split pattern on its strands (split leaf), smooth and shiny to create a basic visualization (background filler) in flower arrangements. This study aims to obtain genetic changes using chemical mutagens. Chemical mutagens used were 250 ppm EMS, 400 and 800 ppm streptomycin, 400 and 800 ppm GA3. The observations were performed after treatments on the parameter of amount of chlorophyll, stomata and leaf color changes. The results showed that both streptomycin treatments significantly reduced the plant height and the number of leaves. The application of streptomycin in high concentration (800 ppm) decreased the total amount of chlorophyll content in the leaves and changed the color as well as the shape of the Monstera leaves. All chemical mutagen treatments had no effect on stomatal density and stomatal area. The changes in leaf color and shape occurred in the high concentration of 800 ppm streptomycin treatment, could not detected by SSR marker.
ANTIBACTERIAL ACTIVITY PROFILE OF MANGROVE ENDOPHYTIC FUNGI ISOLATED FROM BERAU REGENCY, INDONESIA Sukarno
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Mangrove endophytic fungi have the potential to produce secondary metabolites with antibacterial properties. This study aimed to obtain endophytic mold isolates associated with mangrove plants and their antimicrobial activity. Seventeen endophytic molds were isolated from 7 mangrove species originating from Berau, East Kalimantan, Indonesia. Three extracts from the mold isolates OJ-B, OJ-Bu, and 6Xg-Bh showed the highest antibacterial activity with an inhibition zone of 9.63–25.4 mm and with MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values ??of around 0.625–5 and 2.5–10 mg mL–1, respectively. LC-MS (liquid chromatography-mass spectrometry) analysis identified several compounds with antibacterial potential, namely beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide and borrelidin. The 3 species were molecularly identified as Diaporthe phaseolorum, Fusarium proliferatum and Phomopsis sp. Thus, the endophytic molds from several of the mangrove species produced bioactive compounds as antibacterial.
ANALYSIS OF BBM, LEC, AND SERK EXPRESSIONS IN CALLUS OF SUGARCANE (Saccharum officinarum L.) AT SOMATIC EMBRYOGENESIS DEVELOPMENT STAGES Parawita Dewanti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Callus formed in somatic embryogenesis is divided into two types, embryogenic and non-embryogenic callus. Embryogenic callus can be distinguished by the expression of genes as markers related to somatic embryogenesis which are expected to be used as markers to detect callus that has embryogenic capabilities in sugarcane. The aim of this study was to determine the expressions of somatic embryogenesis-related genes in sugarcane. The genes analysis was carried out using somatic embryogenesis callus and using a kit for RNA analysis. Results showed that there were expressions of Baby Boom (BBM), Leafy Cotyledon (LEC), dan Somatic Embryogenesis Receptor Like-Kinase (SERK) gene with specific primer of those three genes, which were collected from embryogenic callus in mass, globular, scutelar, and coleoptilar pre-embryo stages, meanwhile there were no expressions of those genes collected from non-embryogenic callus. Amplification result from PCR product of cDNA using three gene primers detected were in 500 bp for BBM, 400 bp for LEC, and 700 bp for SERK gene.

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