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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Mohammad Ubaidillah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions

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SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Mohammad Ubaidillah
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample.
Pengaruh BAP, IAA, dan Jenis Eksplan terhadap Efisiensi Regenerasi Tomat Fortuna 23 Avivi, Sholeh; Mohammad Ubaidillah; Setiyono; Rifngatul ‘Atiqoh
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.848 KB) | DOI: 10.24831/jai.v50i3.41988

Abstract

Penelitian bertujuan memperoleh protokol regenerasi yang efisien untuk transformasi genetik pada tanaman tomat Fortuna 23. Penelitian dilaksanakan mulai Agustus 2021-Februari 2022, bertempat di Laboratorium Kultur Jaringan Fakultas Pertanian Universitas Jember. Penelitian terdiri dari dua rancangan percobaan yaitu menggunakan RAL faktorial 3 faktor (tahap induksi tunas) diulang sebanyak 3 kali dan RAL non faktorial (tahap induksi akar) diulang sebanyak 5 kali. Perlakuan yang diberikan untuk tahap induksi tunas adalah BAP (0, 1, 2, dan 3 mg L-1), IAA (0, 0.1, 0.2, dan 0.3 mg L-1), dan jenis eksplan (kotiledon, hipokotil, dan akar). Perlakuan yang diberikan untuk tahap induksi akar adalah NAA (0, 0.1, 0.5, dan 1 mg L-1). Pada tahap induksi tunas kombinasi perlakuan B2A3E1 (BAP 2 mg L-1 + IAA 0.3 mg L-1 + kotiledon) merupakan perlakuan yang terbaik karena mampu menghasilkan jumlah tunas terbanyak (8 tunas), dengan awal muncul tunas 21 hari setelah induksi (HSI) dan awal muncul kalus 7 HSI. Pada tahap induksi akar perlakuan NAA 0.5 mg L-1 menghasilkan awal muncul akar tercepat (3 HSI), jumlah daun terbanyak (5 daun), dan jumlah akar terbanyak (26 akar). Kata kunci: induksi akar, induksi tunas, regenerasi
Co-Authors Rifngatul ‘Atiqoh setiyono Sholeh Avivi