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Jurnal Proteksi Agrikultura
Published by Universitas Lampung
ISSN : -     EISSN : 30893240     DOI : https://doi.org/10.23960/jpa.1222-26
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry
Jurnal Proteksi Agrikultura (JPA) publishes research articles related to plant pests, plant pathogens, and the management of plant damage caused by these pests and pathogens in tropical and subtropical regions. In addition to original research papers, JPA also features short communications and unpublished reviews. Manuscripts submitted for publication undergo rigorous peer review. The journal is published biannually in May and November by the Plant Protection Department at the Faculty of Agriculture, Universitas Lampung, Indonesia, in collaboration with the Entomological Society of Indonesia and the Indonesian Phytopathological Society.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Tumbuhan
Articles 25 Documents
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Eksplorasi dan uji patogenisitas jamur entomopatogen di PTPN VII Unit Bunga Mayang sebagai pengendali hayati hama penggerek batang tebu (Chilo sacchariphagus) Rahmawati, Eva; Hasibuan, Rosma; Fitriana, Yuyun; Wibowo, Lestari
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.131-9

Abstract

This research aims to obtain entomopathogenic fungal isolates and determine the characteristics of isolates that have the potential as biological control agents for Chilo sacchariphagus. The research was conducted in the field and at the Protection Laboratory of PT Perkebunan Nusantara VII Bunga Mayang Unit and at the Biotechnology Laboratory of the Faculty of Agriculture, University of Lampung. This research method consists of two sub-sections; the first is the exploration of entomopathogenic fungi, the second is the morphological identification of entomopathogenic fungal isolates isolated from soil suspensions and bait insects and characteristic tests consisting of growth, sporulation, viability and pathogenicity tests against C. sacchariphagus in vitro arranged in a randomized block design (RBD). From the results of the research, six fungal isolates were obtained, two isolates was able to be identified, as Trichoderma sp., and as Aspergillus sp., while the other four isolates could not be identified. Based on the growth, sporulation, spore viability, and pathogenicity tests against C. sacchariphagus that have been carried out, the six isolates have varying characteristics. The TC (Trichoderma sp.) isolate had the highest fungal colony growth, which was 8,50 cm in diameter, the highest sporulation (3,96 x 107 spores/mL) was TNH3BB isolate, and the highest spore viability (100%) were belong the TNH3BB and TC (Trichoderma sp.) isolates, while the TNH2BA (Aspergillus sp.) isolate was able to cause the highest mortality to up, 93,33%.
Uji Efektivitas Trichoderma asperellum dalam Pengendalian Nematoda Puru Akar (Meloidogyne spp.) Secara In Vitro Indriyani, Sisi; Swibawa, I Gede; Dewi, Ni Kadek Emi Sintha; Ginting, Cipta
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1315-20

Abstract

Root-knot nematodes (Meloidogyne spp.) are significant pests that damage the root systems of plants and are difficult to control using conventional methods. The use of chemical nematicides carries the risk of environmental contamination, so environmentally friendly control alternatives are needed, one of which is using the biological agent Trichoderma asperellum. This study aims to determine the effect of spore concentration and exposure duration of T. asperellum on the hatching of Meloidogyne spp. eggs in vitro. The research was conducted at the Plant Pest Science Laboratory and the Biotechnology Laboratory of the Faculty of Agriculture, University of Lampung, from May to July 2025. The experiment used a completely randomized design (CRD). The first experiment tested five concentrations of spore suspension dilutions (10-1, 10-2, 10-3, 10-5, and a control), while the second experiment tested exposure durations of 10, 15, 30, and 60 minutes. The results showed that a concentration of 10-2 (2.7 × 105 spores/mL) was able to inhibit egg hatching by 50.90%, which was higher than the other concentrations. In the duration test, juvenile mortality increased over time, reaching 85.37% at 10 minutes and 99.83% at 60 minutes. In conclusion, a 10-2 concentration with a minimum exposure of 30–60 minutes is effective in reducing the hatching rate of Meloidogyne spp. eggs and has the potential to be applied as an environmentally friendly biological control.
Pengaruh konsentrasi bakteri entomopatogen Bacillus thuringiensis terhadap mortalitas larva kumbang tanduk (Oryctes rhinoceros) secara in vitro Ramadhani, Suci; Pramono, Sudi; Purnomo, Purnomo; Sudarsono, Hamim
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1310-14

Abstract

The rhinoceros beetle (Oryctes rhinoceros L.) is a major pest of oil palm that can reduce production and even cause mortality in immature plants. An environmentally friendly alternative for its control is the use of entomopathogenic bacterium Bacillus thuringiensis (Bt). This study aimed to determine the effect of different Bt concentration on the mortality of O. rhinoceros larvae, identify the most efficient concentration, and estimate the lethal concentration (LC50), and lethal time (LT50). The experiment consisted of six treatments (0%, 4%, 5%, 6%, 7%, and 8%) with four replications. Observed variables included morphological changes, larval mortalty, LC50, and LT50 values. The results showed that Bt had a significant effect on larval mortality. The 7% concentration was the most efficient, resulting in 98.8% mortality at 14 days after application (DAA) with the fastest LT50 of 7 days. The 6% and 8% treatments also achieved 100% mortality at 20 DAA but had longer LT50 (9 days). Symptoms of infection were characterized by larval bodies becoming darkened, softened, and emitting a foul odor. It can be concluded that the 7% Bt concentration was the most efficient in controlling O. rhinoceros larvae in vitro and has potential as a biological control agent to replace chemical insecticides.
Pengaruh implementasi Netamax-FP Unila terhadap keragaman dan kelimpahan nematoda dalam tanah pada pertanaman guava kristal di Lampung Saharani, Aulia Shalsha; Swibawa, I Gede; Helina , Selvi; Suharjo, Radix
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1321-30

Abstract

Crystal guava (Psidium guajava L.) is a horticultural plant whose fruit is widely known and as a favorable fruit in Indonesia. Crystal guava productivity in Lampung is relatively lower compared to that in Central Java and East Java. One of the factors causing a decline in crystal guava production is pest attack, including plant-parasitic nematodes. The aim of this study was to determine the effect of Netamax-FP Unila bionematicide application on the composition of the nematode community and its effects on the diversity and abundance of nematodes in crystal guava plantations at PT Great Giant Food. The research was conducted from September 2023 to March 2024. Nematode observations were carried out before and after application bionematicide. Nematodes were identified to the genera taxonomic level. Nematode diversity was measured using the number of genera, funtional groups, the Shannon-Wiener index, species richness index, species evenness index and Simpson's index. Nematode abundance was measured base on the number of individuals of each genus. The research results showed that 16nematode genera were identified. These genera were classified into three functional groups, including parasitic nematodes (herbivores), bacterial-feeding nematodes (bacterivores), and fungal-feeding nematodes (fungivores). The application of the Netamax-FP Unila bionematicide combined with compost reduced the abundance of plant-parasitic nematodes without having a negative impact on free-living nematodes or overall nematode diversity.
Deteksi dan Identifikasi Mikroorganisme Terbawa Benih Cabai Rawit (Capsicum frutescens L.) yang dijual pada Marketplace Graceia, Stepangel Mighty; Dirmawati, Suskandini Ratih; Nurdin , Muhammad; Aeny, Titik Nur
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1331-40

Abstract

Seed quality is a critical factor in achieving optimal crop yield. Cayenne pepper (Capsicum frutescens L.) seeds are widely obtained through marketplaces; however, information on seed-borne microorganisms associated with commercially sold seeds remains limited. This study aimed to detect and identify microorganisms carried by cayenne pepper seeds marketed through local marketplaces. In this research, five seed varieties obtained from the marketplace were examined, namely KSD 108, Top Garden Seed, Red Cluster Pepper, Raja, and Home Garden. Seed health testing was performed using seed washing, blotter paper incubation, and agar plate methods. Observed variables included seed germination percentage, incidence of microbial infection, fungal identification based on microscopic characteristics, and bacterial identification using the 3% KOH Gram test, soft rot assay, and hypersensitive reaction test. Data were analyzed descriptively and presented in tables and figures. The results showed that uncertified seeds—Top Garden Seed, Red Cluster Pepper, Raja, and Home Garden—were contaminated by various seed-borne microorganisms, predominantly Aspergillus niger, A. fumigatus, A. tamarii, Fusarium oxysporum, and F. verticillioides. Gram-negative bacteria were also detected. In contrast, certified seeds contained A. niger and Gram-positive bacteria.

Page 3 of 3 | Total Record : 25

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