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SIMBIOSIS
Published by Universitas Udayana
ISSN : -     EISSN : 26567784     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Simbiosis (e ISSN: 2337-7224))is an electronic scientific journal published periodically by the Biology Under Graduade Program, Faculty of basic and Natural Sciences, Udayana University , containing scientific works biology that include botany, zoology, microbiology, and molecular genetics and the environment. The Journal is published twice a year, in March and September.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 12 Documents
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Search results for , issue "Simbiosis Volume 13 No. 1 2025" : 12 Documents clear
POTENSI FILTRAT JAMUR Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Aspergillus sp. PADA PAKAN JAGUNG (Zea mays L.) Ni Made Fiona Ranika; Ni Made Gari; Anak Agung Ketut Darmadi
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i01.

Abstract

Corn is a crop that is used as a strategic commodity for Indonesia as it can be used as animal feed. However, corn production is easily decreased due to the presence of Aspergillus sp. fungi which is able to produce aflatoxin that can affect the livestock health problems. Based on this issue, a method of controlling Aspergillus is needed by using Trichoderma sp. fungus filtrate which has antagonistic properties to pathogens. The purpose of this study was to determine the potential of Trichoderma sp. fungus in inhibiting the growth of Aspergillus sp. isolated from corn grain feed, to obtain the concentration of Trichoderma sp. filtrate that is optimal in suppressing the growth of Aspergillus sp. pathogens and to determine the comparison of positive control inhibitory power with Trichoderma sp. filtrate treatment on Aspergillus sp. pathogens. This study used a Complete Randomized Design (RAL) with dual culture methods and diffusion wells with different doses, namely 10% (v / v), 25% (v / v), 40% (v / v), 55% (v / v), and 70% (v / v). The results showed that the fungus and Trichoderma sp. filtrate had a significant effect in inhibiting the growth of Aspergillus sp. (P ≤ 0,05). The percentage of inhibition of Trichoderma sp. in inhibiting the growth of Aspergillus sp. with dual culture method was 68,61 ± 0,83%. Another study of Trichoderma sp. filtrate test on Aspergillus sp. produced the largest clear zone in the 70% treatment with a diameter of 14,81 ± 0,45 mm and the smallest was found in the 10% treatment with a diameter of 9,06 ± 0,55 mm, while in the positive control treatment a diameter of 19,53 ± 0,94 mm was obtained. The filtrate capability is expected to be a solution for related parties in improving the quality of corn grain feed.
EKSTRAKSI DNA DAUN KUMIS KUCING (Orthosiphon stamineus Benth.) KERING Gusti Ayu Putu Intan Pandini; Made Pharmawati; Made Ria Defiani
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.

Abstract

Orthosiphon stamineus Benth. is known as “kumis kucing” is one of the traditional medicinal plants widely used in Indonesia. Morphological identification of dried “kumis kucing” leaves as a herbal remedy is insufficient to prove the existence of counterfeiting practices, thus a more specific authentication method such as molecular genetic identification is required. However, before performing PCR, the selection of DNA extraction method with optimal quantity and quality is necessary. Therefore, this study aims to compare the effectiveness of the Doyle & Doyle method using CTAB, the Dellaporta method using SDS, and the Rogers & Bendich method in extracting DNA from dried “kumis kucing” leaves with the hope of providing information about the best DNA extraction method that can be used to identify the authenticity of “kumis kucing” leaves more accurately. The research method begins with sample preparation, DNA extraction, DNA quantity testing, electrophoresis, and visualization using a UV Transilluminator. Based on this research, the effective method for extracting DNA from dried “kumis kucing” leaves is the modified CTAB method (Doyle & Doyle, 1990), as it yields a high DNA concentration of 1508,33 ng/µL and has a faster processing time, taking 16 hours and 20 minutes. The visualization results show clear and thick DNA bands; however, the resulting DNA is not pure (A260/230: 1,01 and A260/280: 0,59).
PEMANFAATAN LIMBAH AIR CUCIAN BERAS SEBAGAI PUPUK ORGANIK CAIR UNTUK MENINGKATKAN PERTUMBUHAN TANAMAN TOMAT (Solanum lycopersicum L) Windi; Ni Made Susun Parwanayoni; Ida Ayu Astarini
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i03.

Abstract

Washing rice in the rice cooking process produces waste in the form of rice washing water. Rice washing water is kitchen waste which contains nutrients and growth hormones for plants. is an experimental study that aims to explore the content and administration of POC from rice washing wastewater with the addition of molasses and EM4 as organic fertilizer at different concentrations on the growth of tomato plants (Solanum lycopersicum L). Completely Randomized Design (CRD) was used with 4 treatments (P0 = Control (Ordinary water), P1 = 1 liter of rice washing water waste + 100 mL molasses + 100 mL EM4, P2 = 3 liters of rice washing water waste + 100 mL molasses + 100 mL EM4, P3 = 5 liters of rice washing water waste + 100 mL molasses + 100 mL EM4) with 7 replications. The data obtained were analyzed using analysis of variance (ANOVA) with SPSS version 23. The results showed that the POC of rice washing water waste had a significant influence on the height of the tomato plant with 87.57 cm, the number of branches on the tomato plant with 16 branches, the number of fruit with 13 pieces, and the weight of the fruit with the tomato plant with 624.28 g, but had no significant effect on the wet weight and dry weight of the tomato plant. Giving POC concentration to treatment P1 gave the most optimal results compared to other treatments. Keywords: Tomato washing water waste, Liquid Organic Fertilizer
POLA PERTUMBUHAN SISWA DI SD LABORATORIUM UNDIKSHA SINGARAJA DAN SDN 3 PANJI, KABUPATEN BULELENG, BALI Ni Nyoman Dian Anjani; Ni Made Rai Suarni; Dwi Ariani Yulihastuti
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i04.

Abstract

Growth is a change that occurs quantitatively that can be observed in human physique, such as height, and body weight. The purpose of this research was to determine the growth patterns of student at Elementary School (SD) LAB Undiksha Singaraja and SDN 3 Panji. The research method used purposive sampling, carried out at SD LAB Undiksha Singaraja and SDN 3 Panji with 60 probands aged 10-12 years in January 2023. Observations were made on student height, student weight, student Body Mass Index (BMI), student age at SD LAB Undiksha Singaraja and SDN 3 Panji. Data analysis used the T Test. The results of statistical analysis show that there are insignificant differences in the growth patterns of students at SDN 3 Panji and SD LAB Undiksha Singaraja. The research results showed that the average height and weight of students at SDN 3 Panji was lower than at SD LAB Undiksha Singaraja. The majority of students' BMI at SDN 3 Panji is in the underweight category, namely 60% for boys and 66.67% for girls. The majority of students' BMI at SD LAB Undiksha Singaraja is in the normal category, namely 46.67% for boys and 53.33% for girls. Keywords: Body Mass Index, Panji, growth, Undiksha
STRUKTUR DAN KOMPOSISI VEGETASI GULMA PADA TANAMAN JAGUNG (Zea mays L.) DI DESA SEDANG, KABUPATEN BADUNG, BALI Ni Komang Nadya Maharani; Martin Joni; I Ketut Sundra
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i05.

Abstract

Corn is the main food commodity that contains carbohydrates that the body needs and can improve the country's economy. In the production of corn, there are problems, namely the presence of weeds as pest organisms (OPT) which cause a decrease in quality and quantity. Based on this, the aim of this research is to determine the structure and composition of weeds associated with corn plants in Sedang Village, Badung Regency, Bali. The research was carried out using the quadratic method (Quadrat Sampling Technique) in the form of bush plots measuring 5m x 5m and herbaceous plots measuring 1m x 1m. Samples were taken at 4 planting time intervals and 5 repetitions. The ecological parameters used to analyze vegetation are the Importance Value Index (INP), Shannon-Wiener Species Diversity Index (H), Sorensen Similarity Index (IS), and Species Distribution Pattern. The research results showed that the composition of weed types consisted of 24 species and 11 families. The type that dominates the corn cultivation land in Medium Village is Cynodon dactylon weed (L.) Pers, with an INP value of 34.27% and Cyperus rotundus L. of 21.23%, where both types of weed are classified as vicious weeds that emit toxic allelopathic compounds. The Shannon-Wiener Species Diversity Index (H) is classified as a high weed, ranging from 0.70 to 3.74. The Similarity Index (IS) is in the low category, ranging from 19.23-26.79%. The distribution pattern of weed species ranges from 1.22 to 1.46, which indicates that it is clustered because the V/M value is > 1.
PENINGKATAN PERTUMBUHAN VEGETATIF TANAMAN CABAI MERAH (Capsicum annuum L.) SETELAH APLIKASI EKOENZIM DAN PENYIRAMAN SECARA BERKALA Oktavia Cintya Firnawati; Martin Joni; Made Ria Defiani
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i06.

Abstract

The growth of red chili plants (Capsicum annuum L.), is impacted by both environmental factors and nutrition availability. Ecoenzymes have the ability to lower global warming and meet plant nutrient needs. The research was conducted to analyze the vegetative growth of red chili plants after being given ecoenzyme treatment and gradual watering. Red chili seeds are sown for 4 weeks, then transferred to polybags. The research used a two-factor factorial randomized block design method, namely administering ecoenzymes with a concentration of 0%; 0,5%; 1%; 1,5% and watering gradually by giving water every day; twice a day; once in three days. Every combination of treatments was carried out three times. The study's findings demonstrated that, two weeks after seeding, an ecoenzyme concentration of 1,5% could provide the maximum germination capacity (90%) of any concentration. Age 12 Weeks After Planting in polybags, the maximum plant height (70,16 cm) at 0% ecoenzyme concentration and daily watering; the quantity of primary branches (10,78 branches) at 1,5% ecoenzyme concentration and daily watering. Age 16 Weeks After Planting, the largest leaf area (105,13cm2) measured using Image-J when applying an ecoenzyme concentration of 0% and watering every day (control); root length (28,33cm) in the treatment of 1,5% ecoenzyme concentration and watering every three days; The shoot dry weight was the greatest (3,29 g) and the root dry weight was the greatest (0,67 g) when the ecoenzyme concentration was 1.5% and watered every day. Treatment with an ecoenzyme concentration of 1.5% and daily watering exerts the best influence on the growth of red chili plants. Keywords: Liquid fertilizer, leaf area and image-J
VARIASI POLA SIDIK JARI PADA MASYARAKAT DESA DAUP KINTAMANI, BANGLI-BALI Made Nanda Equilla Putri; I Ketut Junitha; Ni Nyoman Wirasiti
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i07.

Abstract

Daup Village is one of the traditional villages in the Kintamani highlands that still carries out religious rituals, the structure of ancient Balinese society. Daup Village is an expansion or separation from the traditional village of Selelulung. Selulung Village in its history is part of the gebog domas community with the main temple, namely the Panarajon Temple which consists of the gebog satak Sukawan, Kintamani, Batang and Selulung.This study aims to determine the variation of fingerprint patterns and compare ID, IF and the number of male and female community tendrils in Daup Village, Kintamani, Bangli, Bali. In this study as many as 59 Probandus from Daup Village were used consisting of 30 men and 29 women who did not have family relations. The Purposive Sampling Method was used in this study which was conducted in November-December 2023. The results of the fingerprint examination showed that there were six fingerprint patterns on the samples of women and male villages with different frequencies and uneven distributions in the five fingers from both hands. The Dankmeijer Index (ID) for the female group is 19,527, slightly higher than the male group of 18,232. Conversely, the Furuhata Index (IF) for the male group is 210,465, higher than the female group, 192,045.The average number of TRC was 141.86 in males and 150.27 in females in Daup Village, which was not statistically significantly different. Based on fingerprint data Daup and Selulung communities. Are diferent. Kata Kunci: fingerprints, arch, loop, whorl, Indeks Dankmeijer, Indeks Furuhata, Daup Village
DIVERSITAS ODONATA DI KAWASAN EKOWISATA SUBAK SEMBUNG, DENPASAR UTARA Ni Luh Putu Nadia Febryanti; Ni Made Suartini; Sang Ketut Sudirga
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i08.

Abstract

The Odonata order comprises two suborders: dragonflies (Suborder Anisoptera) and damselflies (Suborder Zygoptera). This research aim to detect the species and level of diversity of Odonata present in the Subak Sembung Ecotourism Area, located in the northern region of Denpasar. The research was carried out in June until July 2024 using an exploratory method at three observation points in different Odonata habitats. Each habitat was explored in the morning from 08.00 to 11.00 WITA and in the afternoon from 15.00 to 17.00 WITA. Sampling was repeated three times. Odonata samples obtained from the three habitats were then identified through the observation of morphological characteristics. Enviromental factors were measured at each point and sampling, including altitude, air temperature and humidity. Based on the results of the research carried out six spesies of Odonata were found, namely Ischnura senegalensis, Agriocnemis femina, Agriocnemis pygmaea, Pseudagrion pruinosum, Orthetrum sabina and Crocothemis servillia. Odonata data from three different habitats were calculated by diversity index, which was categorized as moderate in the kale habitat with a value of 1,36, while the water henna habitat and rice habitat were categorized as low with values of 0,55 and 0,79. Keyword: Dragonflies, ecotourism, diversity, Odonata, Subak Sembung
PENGARUH PEMBERIAN VARIASI PAKAN FERMENTASI TERHADAP PERTUMBUHAN DAN KEPADATAN ROTIFERA (Brachionus plicatilis ) UNTUK PAKAN NENER IKAN BANDENG (Chanos chanos) Mohamad Mediko; Ni Wayan Sudatri; Deny Suhernawan Yusup
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i09.

Abstract

Brachionus plicatilis is one of the zooplankton that is needed as food for fish larvae. So it is expected that the use of this alternative feed can help hatchery farmers to increase Rotifera production and increase the production of high quality nener. The objectives of the study were to determine the growth of Rotifera in cultures given variations of 4 additional feed ingredients fermented from chicken feces, wheat bran, rice bran and corn bran and to determine the growth response of milkfish (Chanos chanos) seeds fed with Rotifera from the best culture. The additional feed treatment given consisted of 5 factors (4 treatments and 1 control) fermented dried chicken feces, wheat bran, corn bran and rice bran, while the control was phytoplanton from UD Chlorella culture. The fermentation material used was EM4 (Efective Microorganisms 4 from Pt. Songgolangit Persada) The design used was a Randomized Group Design (RAK) with 4 replicate cultures. Giving variations of 4 feed ingredients that have been fermented affects the growth of Rotifera cultures. Growth can be seen from the density of each variation of 4 fermented ingredients namely wheat bran 276,750, corn bran 422,050, rice bran 567,650, chicken feces 169,450, Phytoplankton 77,300. Giving Rotifera cultured with rice bran to the growth of milkfish larvae influenced their growth. Milkfish larvae can grow after being given fermented Rotifera, but the SR value is very low. Milkfish larvae fed with fermented Rotifera had a low SR value of 32,73% mortality rate of 1,373 of 4,500 fish, compared to the SR value of Phytoplanton which was high at 78,15% mortality rate of 3,517 of 4,500 fish. Keywords: Cultivation, Bran, Feces, Culture, Rotifera
KONDISI FISIOLOGIS MAHASISWA RANTAU DARI JAWA DI TAHUN PERTAMA KULIAH DI FAKULTAS MIPA UNIVERSITAS UDAYANA Ayesha Audreyhan; Ida Bagus Made Suaskara; I Ketut Muksin
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i10.

Abstract

Physiological conditions are normal body functions in humans that undergo adaptation and refer to physical changes, especially in probandus who are migrating in a new environment. Students who migrate often experience various physiological condition challenges, such as changes in the amount of hemoglobin levels, blood pressure, and body weight. This study aims to determine the physiological conditions between male and female students who migrate from Java in the first year of study at the Faculty of Mathematics and Natural Sciences, Udayana University. The method of data collection was carried out in a qualitative way, namely interviews and quantitative ways, namely hemoglobin level tests, blood pressure tests, and weight measurements taken through male and female probandus. The results showed that hemoglobin levels in female probandus in the first year of college increased significantly compared to male probandus, which showed that female probandus were more able to adapt to the new environment. Blood pressure checks during the fasting month did not show significant changes in both female and male probandus, due to changes in diet during fasting that affect metabolism. Body weight before and after migrating also did not experience significant changes, which indicates changes in diet, physical activity and the adaptation of body metabolism as well as balanced calorie intake are factors that influence body weight. Keywords: psychological condition, overseas student, udayana university.

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