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Uji Teratogenik Ekstrak Minyak Biji Jintan Hitam (Nigella sativa) pada Mencit (Mus musculus l.) Bunting SETYAWATI, IRIANI; SUDATRI, NI WAYAN
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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This experiment was performed to examine the effects of black cumin (Nigella sativa) seed oil extract onreproduction performance and fetus development if given during organogenesis period. Forty pregnantmice were randomly divided into four groups. Extract was treated orally by gavage with doses 0%(control), 0.1 ml, 0.2 ml, and 0.3 ml since gestation day 6 to 15. Caesarean section were performed topregnant mice on day 18 to remove fetuses. Observation covered reproduction performance i.e. the foetusnumber (reabsorptions, dead/ alive foetus), and morphological of the foetus (weight and length of thelitter, and malformations). Statistical analysis was performed using Anova and Duncans Multiple RangeTest. Teratogenic effects of black cumin oil extract caused decreasing of reproductive performance of themother (alive foetus decreased, dead and resorbed foetus increased) and an increased incidence of birthdefects (fetal weight and length and fetal hemorrhage).Keywords: Nigella sativa, congenital malformations

THE DEVELOPMENT OF OVARIAN FOLLICLE CELLS AND CORPUS LUTEUM OF MICE (Mus musculus) SWISS WEBSTER GIVEN Leucaena leucocephala LEAF EXTRACT Ngurah Intan Wiratmini; Ni Wayan Sudatri; Iriani Setyawati
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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This research was conducted to determine the effect of Leucaena leucocephala leaf extract on the development of ovarian follicle cells of mice (Mus musculus). Twenty four female mice, 10 weeks old, were divided into 4 groups. The control group (P0) were given 0.9% NaCl and the treatment group P1, P2 and P3 were given 0.5, 1 and 1.5 g/ kg bw of Leucaena leaf extract, respectively. The treatments were administered daily for 15 days by gavage. After the treatment ended, all mice were dissected to collect the ovaries. Histological preparation of the ovaries used paraffin method and Hematoxylin-Eosin staining. Observations were made on the number of primary, secondary, and tertiary oocytes, and also De Graaf follicles and corpus luteum. Data were statistically analyzed using One Way ANOVA method. The results showed there were no significantly differences (P>0.05) on the number of primary, secondary, and tertiary oocytes, as well as De Graaf follicles and corpus luteum among the control and treatment groups. The mimosine content of Leucaena leaf extract was 0.87% (less than 1%) so it could not inhibit the secretion of Follicle Stimulating hormone by the pituitary gland. The doses of Leucaena leaf extract used in this research did not significantly affect the follicles development of mice ovaries. In this study, the mimosine content of Leucaena leaf extract decreased 91.8%.

PELATIHAN PEMBUATAN BODY CREAM DAN LOTION LIDAH BUAYA (ALOE VERA) DI PURI DAMAI DESA SINGAKERTA KECAMATAN UBUD KABUPATEN GIANYAR BALI N.L. Arpiwi; I.K. Muksin; I.G.A.S. Wahyuni; I.K. Ginantra; N.W. Sudatri
Buletin Udayana Mengabdi Vol 18 No 4 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Lidah buaya (Aloe vera) adalah tanaman sukulen dari keluarga Asphodelaceae yang tersebar luas di daerah tropis dan sub-tropis. Tanaman ini sangat populer karena memiliki banyak kegunaan, misalnya sebagai pelembab kulit, pertumbuhan rambut, dan pengendalian jerawat. Tujuan dari pelatihan ini adalah untuk memberikan informasi kepada masyarakat Desa Singakaerta, Kabupaten Gianyar, tentang bagaimana mengolah lidah buaya menjadi body cream dan lotion sehingga dapat meningkatkan nilai ekonomi tanaman tersebut. Prosedur kerja dimulai dengan mengupas daun lidah buaya untuk mendapatkan pulp, lalu diblender dan disaring untuk mendapatkan jus. Basis cream dan lotion terdiri dari minyak kelapa murni (VCO), cethyl alcohol, asam stearat, gliserin, triethanolamine, nipagin, nipasol dan aquades dengan jumlah sesuai formulasi. Fase air dicampur dengan fase minyak pada suhu 70oC sambil diaduk sampai homogen dan kemudian ditambahkan jus lidah buaya pada suhu 40oC. Aroma cendana ditambahkan untuk body creamdan aroma melati ditambahkan ke body lotion pada suhu 30oC. Body cream berwarna putih susu, aroma cendana, tekstur lembut, agak kental, pH 6,5, homogen dengan viskositas 95 poise. Body lotion berwarna putih susu, aroma melati, tekstur lembut, lebih encer, pH 7, homogen dengan viskositas 39 poise. Kata kunci : Body cream, body lotion, Aloe vera, VCO

UPAYA MENINGKATKAN KEMAMPUAN PROBLEM SOLVING SISWA SD ADHI MEKAR INDONESIA MELALUI PEMBELAJARAN MATEMATIKA BERBASIS EKSPLORASI I. Harini; I G.S. Astawa; N.W. Sudatri; I G.A.P. Adnyana; I M. Widiartha
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Diantara semua mata pelajaran SD, pembelajaran matematika tetap menjadi topik yang paling sering muncul sebagai pembelajaran yang paling bermasalah dalam proses belajar mengajarnya. Kesiapan guru dalam mengajar dan kurang minat siswa dalam belajar matematika merupakan dua masalah penting yang harus diatasi. Untuk membuat pembelajaran matematika lebih bermakna bagi siswa, maka pengintegrasian mata pelajaran matematika dengan masalah-masalah nyata sangat penting. Salah satu alternatif yang dapat ditempuh untuk menyelesaikan masalah tersebut adalah dengan merancang pembelajaran matematika yang berbasis eksplorasi. Kegiatan pengabdian ini menggandeng SD Adhi Mekar Indonesia sebagai sekolah mitra. Adapun tujuan yang ingin dicapai melalui kegiatan ini diantaranya: (1) guru dapat mengembangkan perangkat pembelajaran matematika yang lebih inovatif berbasis eksplorasi (2) melihat sejauh mana peningkatan kemampuan problem solving siswa melalui pembelajaran berbasis eksplorasi. Dari hasil analisis tabulasi silang antara nilai pre test dan post test menunjukkan bahwa pemberian pelatihan pembuatan bahan ajar berbasis eksplorasi berpengaruh signifikan terhadap peningkatan kemampuan guru dalam membuat bahan ajar berbasis eksplorasi.

JENIS-JENIS SERANGGA DI SEKITAR PANTAI PERERENAN, MENGWI, KABUPATEN BADUNG Ni G. A. Manik Ermayanti; Ni W. Sudatri; A. A. G. Raka Dalem
Bumi Lestari Journal of Environment Vol 7 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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A Study on insects was carried out on Pererenan, District of Mengwi, Badung Regency, Baliprovince, between September and December, 2006. Samples were captured using insect nets. Thesamples were then identified for determination of their species. Their roles on the ecosystem weredescribed. Seven species of butterflies and 29 kinds of other insects were identified on this study.From those, none to be classified as protected species.

IDENTIFIKASI MAKROZOOBENTHOS DI TUKAD BAUSAN, DESA PERERENAN, KABUPATEN BADUNG, BALI Ni Made Suartin; Ni Wayan Sudatri; Made Pharmawati; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 5 No 1
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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A macrozoobenthos study was conducted at Bausan River, Pererenan village, Badung regency, Bali between September and December, 2006. There were six sampling stations were determined purposively. At each station, five unit of square plots of 40 cm x 40 (in maximum solum depth) samples were taken. The result showed that there were seventeen species of macrozoobenthos were found. There was no protected species found in this study. The macrozoobenthos had 2,28 level of index diversity. This indicated that the ecosystem at this area was in a stable condition with a medium level of macrozoobenthos diversity.

MORFOMETRI DAN KARAKTERISTIK FISIK TUMISI (Amphidromus peversus) DARI KAMPUS UNIVERSITAS UDAYANA BUKIT JIMBARAN, BALI-INDONESIA N. W. Sudatri; N. M. Suartini; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 2 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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A study on mollusks (Amphidromus peversus) has been carried out on Udayana University, BukitJimbaran (Bali, Indonesia) campus between November, 2003- November, 2005. The study was undertakenon a secondary forest on a limestone dry soil with dominant vegetation of Glerecydium sepium, Lantanacamara, Ceiba pentranda, and Acasia auriculiformis. Samples were randomly taken on quadrate samplings.Mollusks were captured on the land surface or on living vegetations. Results of the study showed that A.peversus has an average of shell length of 27.9 mm, shell width 18.0 mm , and body weight 7.90 g, number ofshell whorls 5.3, with the proportion of direction of whorl to the right of 55.3%. The shell of A. peversus hasno operculum. The color of the shell was yellow with brown stripes.The relationship between shell length (X) and with body weight (Y) can be represented by theequation of Y = 0.473 X – 5.32 (R2 = 11.9 %), which showed week relationships between those parameters.Meanwhile the relationships between the shell length (X1), width (X2) and body weight (Y) was best fitted bythe following equation : Y = 0.289 X1 + 0.0140 X2 – 3.47 (R2 = 94.9%).

MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

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This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.

MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5733

This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.

Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomach and Duodenum in Mice Andayani, Ni Kadek Prederika Sari; Setyawati, Iriani; Sudatri, Ni Wayan
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 2 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n2.p52-59

In Indonesia, Mimosa pudica Linn. (M. pudica L.) is a wild plant (weed) from the Mimosaceace family. This plant is widely used as a traditional medicine for various types of diseases such as insomnia, acute eye inflammation, urolithiasis (urinary stones), fever, and bronchitis. This study aimed to find out the effect of M. pudica leaf extract on the histological damage of the stomach and duodenum of mice. This study used a Completely Randomized Design with 24 male mice, which were divided into control placebo (P0) given CMC-Na 0.5%; and M. pudica leaf extract doses of 200 mg/kg body weight (P1); 400 mg/kg body weight (P2); and 600 mg/kg body weight (P3). The extract was administered orally for 28 days. On day 29, the animal was dissected to collect its digestive organs. The histological preparation of the stomach and duodenum of mice was performed by using the paraffin method and Hematoxylin and Eosin staining to observe the histological damage, namely congestion, hemorrhage, epithelial cells desquamation, inflammatory cell infiltration, edema, and hyperplasia. Quantitative data were analyzed with a one-way ANOVA test and Duncan's post hoc test with the SPSS version 22. The results showed that the M. pudica leaf extract increased hemorrhage and hyperplasia damage at doses of 400 and 600 mg/kg body weight in mice’s duodenum.

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