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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol. 3 No. 2 April 2014" : 8 Documents clear
KEANEKARAGAMAN JENIS DAN PEMANFAATAN BAMBU DI DESA LOPAIT KABUPATEN SEMARANG JAWA TENGAH ( SPECIES DIVERSITY AND UTILITY OF BAMBOO AT LOPAIT VILLAGE SEMARANG REGENCY CENTRAL OF JAVA) Dian Setyo Putro; J Jumari; M Murningsih
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lopait Semarang’s regency society own a strong interaction with bamboo. This research aims to identification species of bamboo in Lopait village, and to share information related to the ussage of the bamboo. Data collection is using explorative survey to investigate species of bamboo in Lopait village. The information related to the ussage of bamboo collected by interview. The collected data was tabulled and described. Result data of interview made in tabulation, categorized on benefits, description of benefit’s and managerial. Based on the investigation, there are 6 species of bamboos, i.e.,  apus bamboo (Gigantochloa apus), betung bamboo (Dendrocalamus asper), black bamboo (Gigantochloa atroviolacea), ampel bamboo (Bambusa vulgaris), ori bamboo (Bambusa arundinaceae), dan atter bamboo (Gigantochloa atter). Bamboo’s usage in Lopait village were categorized into 7, they were handicraft (19 speciess of handicraft), building material (3 speciess of building material), food material (1 species of food material), chemical substance (1 species of chemical substance), food wrap (1 species), firewood, and erotion resistance. Lopait’s society own good knowledge on bamboo cultivation and have ability to manage it traditionally, or create it become valuable handicrafts. Keywords : Diversity of bamboo, bamboo utility and management, etnobotany, Lopait village
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI “TEMPOYAK” Arina Aisyah; Endang Kusdiyantini; Agung Suprihadi
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Tempoyak is a food made from fermented durian flesh with the addition of salt and brooded for seven days. Tempoyak quality is strongly influenced by the presence of microbes involved during the process of fermentation. This study aimed to isolate and characterize lactic acid bacteria (LAB) that play a role in the tempoyak fermentation process, and to perform proximate analysis of the durian and tempoyak. Isolation was done on 0, 3rd, 5th, and 7th day fermentation with streak methods. The isolates then underwent morphology and motility observation, biochemical tests, and proximate analysis.  The isolation of tempoyak resulted seven isolates of bacteria that had different colony and cell morphology. Gram staining of the bacterial cell produced a purple color with the rod and spherical shape. Motility test resulted non motile bacteria. Catalase test of bacteria isolates produced negative catalase. Isolates showed positive results in test for acid production and carbohydrate fermentation. pH decrease from 6.88 to 5.74 on the last day of brooding. Seven isolates obtained had characteristics that were similar with the characteristic of LAB, which was Gram positive, rod shaped or spherical, non motile, negative catalase, and produce acid. Tempoyak nutrient contained, ie 15.12% moisture content, 27.03% ash content, 2.69% fat content, 6.37% protein content, and 48.79% carbohydrate content. Keywords: tempoyak, LAB, morphology, biochemistry, proximate
ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA PANGAN FERMENTASI MANDAI Mangasa Tua Pandapotan Siregar; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

East Kalimantan has a lot of processed food products traditionally, one of them is mandai which is fermented food made from fruit leather Cempedak (Arthocarphus champeden). Lactic acid bacteria involved in the fermentation mandai greatly affect the quality of the final product. This study aimed to perform the isolation and characterization of lactic acid bacteria in the mandai fermentation process. MRS medium was used in the isolation of bacteria for 14 days. Isolation of bacteria during the fermentation process mandai obtaining 17 isolates, nine isolates Gram negative isolates, and eight isolates Gram positive. Eight isolates showed positive results against some of the morphological and biochemical characterization. Including biochemical characterization, acid formation and gas production from glucose, motility test, catalase test, growth at 160C and 480C, hydrolysis of starch, fat hydrolysis, and hydrolysis of casein and acid formation test. Eight isolates bacteria made mandai into processed food products that had a sour taste. Keywords: lactic acid bacteria, BAL ,bacterial isolates, cempedak skin.
Pengaruh Pemberian Kombinasi Pupuk Organik Padat dan Organik Cair Terhadap Porositas Tanah dan Pertumbuhan Tanaman Bayam (Amarantus tricolor L.) Imelda Anastasia; Munifatul Izzati; Sri Widodo Agung Suedy
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The porosity of soil is one of the parameters of soil porosity. Tanah Merah in Madura island  has low fertility. This soil not good to used because the physical structure and  has low nutrition. The addition of organic materials from solid and liqiud organic fertilizer can increase soil porosity and spinach  growth. The research aimed to determine the effect of solid, liquid organic fertilizer, and their combination to  increase porosity and spinach growth. The design of the research is completely randomized design (RAL) with a single factor with combination treatment media, control treatment with no treatment to media (P0), media were given solid organic fertilizer (P1),  media were given liquid organic fertilizer (P2), and media were given combination of solid and liquid organic fertilizer (P3). Analysis of the data used  Analysis of Variance ( ANOVA ) continued by DMRT test with significance level of 95%. The results of the research showed organic fertilizer given effect to the increase in porosity of the soil and plant growth, as well as the provision of combination solid and liquid organic fertilizer are the most optimal fertilizer for soil porosity and spinach growth. Keywords : Spinach, solid organic fertilizer, liquid organic fertilizer, soil porosity, spinach growth
UJI AKTIVITAS ANTIBAKTERI EKSTRAK TUMBUHAN Euphorbia hirta L. TERHADAP Ralstonia solanacearum, Escherichia coli, DAN Staphylococcus aureus SECARA IN VITRO Genoveva Preta Angelika; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biocontrol using Patikan kebo (Euphorbia hirta L.) plant is an alternative solution to control pathogenic bacteria. Such wild plant is known to contain active compounds with antibacterial activity such as tannins, alkaloids, flavonoids, saponins, and phenols. This study aims to determine the antibacterial activity of the methanol extract of E. hirta against R. solanacearum, E. coli and S. aureus. The extraction method of E. hirta was maceration with methanol solvent, while antibacterial activity test using the agar diffusion method (Kirby-Bauer) with test bacteria was R. solanacearum, E. coli and S. aureus. E. hirta extract tested was pure extract (100%). Observed response was diameter of inhibitory zone formed around the paper discs that had been smeared with E. hirta extract on the media. Analysis of the data using a Completely Randomized Design (CRD) 1 factor (test bacteria) with three times repetition, followed by a further test of Duncan with 95% confidence level. The results indicated that E. hirta produced extraction yield of 6,45%. Antibacterial activity of E. hirta extract against R. solanacearum, E. coli and S. aureus was indicated by Inhibitory Zone Diameter (HZD), respectively for 21,8 mm, 18,26 mm and 17,06 mm. The results of this study showed that the methanol extract of E. hirta plant had antibacterial activity against R. solanacearum, E. coli and S. aureus, thus can be used as a biocontrol agent of bacterial wilt disease in plants caused by R. solanacearum and human disease caused by E. coli and S. aureus. Keywords: Euphorbia hirta, Ralstonia solanacearum, Escherichia coli, Staphylococcus aureus, antibacterial activity, diffusion method
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.) Deverina Mergypta; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis
OPTIMASI PEMBERIAN PUPUK GRAMAFIX DALAM DEGRADASI CEMARAN MINYAK BUMI OLEH BAKTERI INDIGENOUS SECARA IN VITRO Linda Safitri; Agung Suprihadi; Endang Kusdiyantini; Yeti Darmayati
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Petroleum pollution has bad impact for life in marine ecosystems, particularly in coastal areas. One method of handling the pollution is bioremediation using bacteria that are able to degrade and utilize petroleum hydrocarbons as carbon source. One technique of bioremediation is biostimulation, that is the addition of nutrients that can improve the process of oil degradation by degrading bacteria. Nutrient slow-release fertilizer are used, one of which is Gramafix. The purpose of this study was to determine the optimal concentration of Gramafix in increasing degradation of petroleum contaminants by indigenous bacteria. Four concentrations of Gramafix as treatments are P1 (0,085 g), P2 (0,171 g), P3 (0,341 g) and P4 (0,682 g), as well as the negative control treatment (no fertilizer and bacteria) and positive control (with bacteria, without fertilizer). Observations were carried out  four times, on 0, 7, 14 and 28 days of incubation. The parameters used are heavy oil using the gravimetric method, the total number of bacterial cells using Acridine Orange Direct Counting and environmental factors such as nitrogen contents, phosphorus contents, temperature, dissolved oxygen, pH and salinity. Analysis of the data using a completely randomized design (CRD) with ANOVA and Duncan test. The results showed the P3 can improve the process of oil degradation by bacteria that degrade the highest percentage of 65,91% in the 28 days of incubation, it also has more bacterial cells than other treatments. The result of this study is the addition of 0,341 g Gramafix is optimum for enhancing the degradation of petroleum hydrocarbons by degrading bacteria. Keywords: Pollution, petroleum, bioremediation, biostimulation, slow-release fertilizer.
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI MAKANAN FERMENTASI BEKASAM IKAN MUJAIR (Oreochromis mossambicus Peters) Olivia Nisa Mumtianah; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bekasam is one of the traditional fermented food made from freshwater fish with the addition of salt and carbohydrate sources. Mozambique fish is used as a raw material, because it has high protein content and can be easily obtained in the market at affordable prices. Mozambique fish is used to make bekasam with the addition of salt as much as 18% of the weight of the fish and brooded for 48 hours, then added with toasted rice as much as 15% of the weight of the fish and fermented for 7 days. This study aimed to isolate and characterize lactic acid bacteria and analyze the nutritional value of mozambique fish bekasam. The methods used were  isolation, characterization and proximate analysis of the mozambique fish bekasam. Isolation of lactic acid bacteria was carried out on mozambique fish as a control, mozambique fish before and after the addition of toasted rice. Isolation obtained 6 isolates of Gram positive bacteria, 5 isolates of cocci shaped bacteria and 1 rod shaped bacteria. Based on morphological and physiological tests, six isolates were Gram positive, non motile, catalase negative, positive to produce acid and had proteolytic activity. Bekasam mozambique fish contained as much as 0.64% lactic acid and pH is 5.39. The results of the proximate analysis bekasam mozambique fish showed that the fermentation process increased the nutritional value bekasam quality mozambique fish with 5.5270% water content, 33.0628% ash content, 0.0788% crude fiber content, 45.0546% crude protein content, 7.9419% crude fat content and 13.9407% carbohydrate content. Keywords : bekasam , lactic acid bacteria , isolation, characterization, proximate analysis

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