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Biodiversitas Journal of Biological Diversity
Published by Universitas Sebelas Maret
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "vol. 10 no. 1 (2009)" : 10 Documents clear
Biodiversity Study of Black Coral (Order: Antipatharia) Collected from Manado, Indonesia based on rDNA Internal Transcribed Spacer (ITS) Sequences Analysis HAPRY FRED NICO LAPIAN
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100101

Abstract

Biodiversity of black coral (order Antipatharia) collected from the Bunaken Marine Park (Manado Sea, Indonesia) has been studied based on the sequence of the Internal Transcribed Spacers (ITS) region of rDNA gene. The results of the study showed that the 18 species of Antipatharia were considered to be separated in two family groups, family Myriopathidae and Antipathidae-Aphanipathida. A significant species-specific signal has been detected among the families of Antipathidae andAphanipathida. However, more studies-on different species were required to be clearly interpreted. The new species Pseudocirrhipathes mapia, the new genus Reticulopathes, and possibly a new taxon of the family Myriopathidae has beenrecognized based on ITS sequence data.Key words: biodiversity study, Manado, rDNA ITS, Antipatharia, black coral.
Stomata Diversification and Phylogenenetic Analysis of 13 Species of Family Euphorbiaceae sensu lato TOPIK HIDAYAT; KUSDIANTI KUSDIANTI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100104

Abstract

Investigation on diversity of stomata from 13 species of family Euphorbiaceae has been carried out. Characters like type of stomata, position of stomata, presence/absence of ledge, and density both abaxial and adaxial leaf surface were examined.Stomata characters in the family were found quite diverse in this study. Monophyletic nature of Euphorbiaceae has been proved in this study on the basis of phylogenetic analysis using parsimoni method. Our data further suggested that the family can be classified into two major groups. However, surprisingly, genus Phyllanthus is non-monophyletic.Key words: diversity, Euphorbiaceae, phylogenetic analysis, stomata.
Primer Screening for Dyera costulata (Miq) Hook.f Random Amplified Polymorphic DNA Analyses YUYU SURYASARI POERBA; ELIZABETH ANITA WIDJAYA
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100103

Abstract

Dyera costulata (Miq.) Hook.f (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia, Thailand, Malay Peninsula and Indonesia, especially in Sumatra and Kalimantan (Borneo) islands. Its economic value was in its copious latex, used as gum in the manufacture of chewing gum. Today the timber of this species is much sought after for the manufacture of pencils and picture frames. Information on genetic diversity of the species is very limited. Hence studieswere initiated to screen primers for RAPD analyses of Dyera costulata for use in genetic variation studies. Seventy one Operon primers (10 mer) were used to generate a total of 864 consistent and ambiguous amplification products ranging from 200 bp to 2.0 kb. Rare and genotype specific bands were identified which could be effectively used to distinguish the genotypes. 34 highly polymorphic primers (100%) are recorded from 71 primers used. Three primers (OPA-04, OPU-06, and OPU-07) produced highest variable RAPD profiles. The dendrogram separated the 8 genotypes into 2 groups. Geneticdissimilarity ranged from 0.07 to 0.71 %.Key words: Dyera costulata, jelutong, primer screening, RAPD.
Selection of Indonesia Cassava (Manihot esculenta Crantz) Genotype as Source of β-Carotene DODY PRIADI; DJUMHAWAN RATMAN PERMANA; SRI ELIN DONA; SRI HARTATI; ENNY SUDARMONOWATI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100102

Abstract

Fourteen genotypes of Indonesia cassava (Manihot esculenta Crantz) of two generations were evaluated for β-carotene content. The β-carotene content of tubers and leaves were determined by spectrophotometry method. Other parameterssuch as water and ash contents were also evaluated. Results showed that β-carotene content of tubers of fourth generation(planted in 2006-2007) was higher than that of first generation (planted in 2002-2003), with the exception of Apuy, Iding andSarewen genotypes. β-carotene content of tubers was lower than that in their leaves of fourth generation plants and that there was no correlation between both organs in terms of β-carotene content of tubers and their leaves except for Tim-Tim 40 genotype. β-carotene content of tubers in several genotypes i.e. Kalbar III (1.13 ppm), Local Muneng (1.03 ppm), Tim-Tim 29 (1.61 ppm) was higher than 1 ppm, and the highest value was found in Tim-Tim 40 (16.83 ppm) which was significantly different (5%) with other genotypes. Meanwhile the lowest content was found in Sarewen genotype as it could not be detected. Water and ash contents of the tubers were between 54 and 69% and between 0.20 and 0.79% respectively. As β-carotene is the precursor of vitamin A, consuming high β-carotene tubers are sufficient for daily requirements of vitamin A, although further study is needed.Key words: cassava, Manihot esculenta, genotype, selection, β-carotene, tubers, leaves
Functional Importance of Seed Weight on the Seedling Establishment of Syzygium bankense (Hassk.) Merr. & L.M. Perry and Quercus gemelliflora Blume ANNISA SATYANTI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100106

Abstract

Seedling is the first and prominent phase of plant development. Seedling performance can be determined by seed and itsembedded characteristics, among them is seed weight. In this study we would like to see how seed weight within species affect seedling establishment. The hypotheses tested were that heavier seed is regarded advantageous compared to lighterseed by functionally produce more competitive seedlings for better growth. Heavier seeds indeed resulted in higher shootlength than lighter seeds. The same pattern was also pronounced for the correlation between seed weight and shoot length of Syzygium bankense and Quercus gemelliflora. There was also a significant positive correlation between seed size andnumber of leaf within species.Key words: seed weight, Syzygium bankense, Quercus gemelliflora, seedling establishment, germination
A Comparison on Biodiversity between Private Conservation and Wildlife Reserve Forests in Riau by using Macro-moths as an Indicator HARI SUTRISNO
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100107

Abstract

A study on biodiversity of the two forest management types, private conservation forest PT. A and wildlife reserve forest of Suaka Margasatwa Giam Siak Kecil, Riau by using a rapid assessment approach with macro-moths as an indicator wasconducted from 23 October to 6 November 2007. Four sample sites were established in Giam Siak Kecil Wildlife Reserve,whereas three sample sites were performed in private conservation forest PT. A. The results show that the diversity indexes based on Fisher’s α of the private forest PT. A was higher than those of wildlife reserve forest Giam Siak Kecil, they were 67.98 and 47.86, respectively. The species composition of the two forests is different, pyralid moths dominate at Giam Siak Kecil. On the contrary, Geometrid moths dominate at private conservation forest PT. A. The results indicated that diversity index and species composition in Giam Siak Kecil is influenced by habitat changes and decrease on floral diversity due to illegal logging. Moreover, a low faunal similarity which is indicated by Jaccard’s index that is only 0.218 showed that thesamples represent significant different communities.Key words: biodiversity, conservation, macro-moths, Fisher’s α index
The Effect of Seed Maturity, Temperature and Storage Period on Vigor of Picrasma javanica Bl. Seedling NINIK SETYOWATI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100110

Abstract

A study on the effect of seed maturity, temperature and period of storage on vigor of Picrasma javanica Bl. seedling was conducted at the Macropropagation Laboratory, Research Centre of Biology, LIPI, Cibinong, from March to September 2008. The research was arranged using Randomized Block Design with 3 factors and 3 replications, in which each replication had 20 samples. The first factor was stage of seed maturation with 2 levels i.e. pre-mature and mature stage; the second factor was storage temperature with 3 levels i.e. ambient temperature (28±1ºC), 20ºC and 5ºC; and the third factor was storage period with 4 levels i.e. 0, 1, 2, 3 months. The result showed that the germination time of Picrasma javanica seed was varies between 11-23 days. The mature seeds had better growth as compared to pre-mature seeds. Storage on the ambient temperature (28±1ºC) caused decrease of seed vigor, so is not suggested to seeds store in the ambient temperature. The storage under lower temperature (5ºC and 20ºC) was able to maintain seed vigor until 3 months storage.Key words: seeds maturity, temperature, storage period, vigor, Picrasma javanica Bl. seed.
Biodiversity of Endophytic Fungi Associated with Uncaria gambier Roxb. (Rubiaceae) from West Sumatra MUHAMMAD ILYAS; ATIT KANTI; YULIASRI JAMAL; HERTINA HERTINA; ANDRIA AGUSTA
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100105

Abstract

Endophytic fungi are specific microbes that live at a specific ecosystem in nature. Various endophytic fungi have been known posses an ability to produce a broad range of biologically active substances. Totally 53 endophytic filamentous fungi were isolated from leaves, stems, fruits and roots of the two plant varieties of Uncaria gambier Roxb. (Rubiaceae), i.e. gambir udang and gambir nasi. Morphological observation of 53 isolated endophytic filamentous fungi was further divide into two classes, Coelomycetes and Hypomycetes. Fifteen fungi isolates are unidentified due to lack of specific morphological characters. Morphologically identified fungi at genera level are: Aspergillus, Cladosporium, Diaporthe, Fusarium, Penicillium, Pestalotiopsis, Phoma and Phomopsis. Chemotaxonomic analysis base on their TLC chromatogram patterns of the ethyl acetate extract are in agreement to morphological grouping.Key words: endophytic fungi, Uncaria gambier Roxb., morphological character, chemotaxonomy.
Phagocytosis Effectivity Test of Phenylbutenoid Compounds Isolated from Bangle (Zingiber cassumunar Roxb.) Rhizome CHAIRUL CHAIRUL; PRAPTIWI PRAPTIWI; SOFNIE MARUSIN CHAIRUL
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100108

Abstract

This study was conducted to determine the immunostimulant activity of several phenylbutenoid derivatives isolated frombangle (Zingiber cassumunar Roxb) rhizome: [1] [(E)-4-(3’,4’-dimethoxyphenyl)but-3-en-1-ol], [2] [(E)-4-(2’,4’,5’ trimethoxyphenyl) but-3-en-1-ol] and [3] [(E)-4-(3’,4’,1-trimethoxyphenyl)but-3-en-1-ol]. Immunostimulant activity was done by stimulating macrophage cells of mouse peritoneum. The result showed that [(E)-4-(3’,4’-dimethoxyphenyl)but 3-en-1-ol] compound had highest immunostimulant activity (99.0%) compared to compound 2 (93.7%) and 3 (80.0%).Key words: Zingiber cassumunar Roxb, immunostimulant, phenylbutenoid derivative, [(E)-4-(3’,4’-dimethoxyphenyl)but-3-en-1-ol], [(E)-4-(2’,4’,5’-trimethoxyphenyl)but-3-en-1-ol]and [(E)-4-(3’,4’,1-trimethoxy phenyl)but-3-en-1-ol
The Effect of Crude Extract of Pandanus conoideus Lamb. var. Yellow Fruit on Apoptotic Expression of the Breast Cancer Cell Line (T47D) OKID PARAMA ASTIRIN; MARTI HARINI; NOOR SOESANTI HANDAJANI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100109

Abstract

A mechanism controlling a growing cancer cells is by a programmed cell death (apoptosis). The wildtype-p53 enable to stop cleaves that follow DNA repair or cell death (apoptosis). The mutation of wt-p53 caused loosing its ability to inhibit cancer cells proliferation. Healing methods like surgery, radiation, immunotherapy and chemotherapy still have some weaknesses, and clinical medicine to cancer is also still has any dissatisfactory. Much of chemotherapy was not given optimal result yet, because no specific action to cancer cells only, but also to the normal cells. These problems encourage important effort to find specific and sensitive anticancer. Empirical evidence indicates that the crude extract of Pandanus conoideus Lamb var. yellow fruit has potential effect as an anticancer. Method of Freshney was used in growing T47D cell line, counting cells was done by direct counting, and apoptotic evaluation was done by TUNEL enzymatic labeling assay. The results of theresearch demonstrated that the LC50 of yellow fruit extract are 0.25 μL/mL. The percentage of apoptotic of 0.125 μL/mL,0.0625 μL/mL, and 0.03125 μL/mL are 34.38±2.26, 30.03±3.87 and 21.07±1.14 respectively.Key words: T47D, Apoptotic, p53, Pandanus conoideus Lamb. var. yellow fruit.

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