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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 62 Documents
Search results for , issue "vol. 22 no. 7 (2021)" : 62 Documents clear
Diversity of insect galls associated with Eucalyptus alba & E. urophylla in altitudinal zones in Timor Island, Indonesia Lindung Tri Puspasari; DAMAYANTI BUCHORI; ROSICHON UBAIDILLAH; HERMANU TRIWIDODO; PURNAMA HIDAYAT
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220715

Abstract

Abstract. Puspasari LT, Buchori D, Ubaidillah R, Triwidodo H, Hidayat P. 2021. Diversity of insect galls associated with Eucalyptus alba & E. urophylla in altitudinal zones in Timor Island, Indonesia. Biodiversitas 22: 2667-2679. We investigated the galling insects associated with two species of Eucalyptus, namely E. alba Reinw. and E. urophylla S.T. Blake in different altitudinal zones in Timor Island, Indonesia. This is the first report for Indonesia of the diversity patterns and community structures of galling insects in these two species of eucalyptus and the type of galls they produce. Surveys and data collection were carried out between October 2017 and June 2018 at different altitudes of secondary forest in Timor Tengah Selatan district, Timor. We visited five altitudinal zones (± 829 m asl., ± 942 m asl. ± 1,621 m asl., ± 1992 m asl., and ± 2457 m asl.) and selected 25 eucalyptus plants of 3 to 5 m in height in each altitudinal zone from which to collect insect galls. The galls were observed on leaves, upper leaves (shoots), outer leaves, symptomatic twigs and stems. The galls were taken to the laboratory for rearing of the insects they contained. A total number of 12 gall types were found in E. alba and 15 in E. urophylla from which 28 insect morphospecies were identified belonging to 16 families and five orders. The order Hymenoptera was represented by ten families: Bethylidae, Torymidae, Eulophidae, Eupelmidae Eurytomidae, Pteromalidae, Encyrtidae, Mymaridae, Braconidae and Scelinonidae while the order Diptera was represented by three families: Fergusoninidae, Cecidomyiidae and Chaoboridae. Galling species diversity ranged from 1.71 to 2.38 on the Shannon-Wiener index and 0.75 to 0.87 on the Simpson index and showed that the galling-insect communities were species-rich and composed of galling formers and very few inquilines. In the study, communities are categorized and structured according to different types of galling insects, including those that induce galling, those that are parasitoid, and those that are inquiline. Within the category of gall-forming insects in the two species of eucalyptus, Eulophidae is found to be the dominant family. Our field data makes an important contribution to basic knowledge of insect galling patterns in eucalyptus forests and constitutes baseline data for the implementation of pest control.
Short Communication: The detection of Plasmodium in mosquitoes from Sumba and Sorong districts, Indonesia MUNIRAH MUNIRAH; ISRA WAHID; FIRDAUS HAMID; Sitti Wahyuni
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220716

Abstract

Abstract. Munirah M, Wahid I, Hamid F, Wahyuni S. 2021. Short Communication: The detection of Plasmodium in mosquitoes from Sumba and Sorong districts, Indonesia. Biodiversitas 22: 2680-2684. Malaria is heading for elimination in 2030, but the disease remains prevalent in Indonesia. Therefore, this study aimed to determine a vector other than Anopheles that is potentially responsible for malaria transmission in the country's endemic areas. The mosquitoes were trapped by Kelambu trap, collected using a mouth aspirator, and stored in a tube containing silica gel. They were also examined microscopically following O'Connor identification keys, and Nested PCR was used to detect Plasmodium DNA. The number of mosquitoes collected from two areas was 1.336. In Sumba (Gaura village) district, 493 mosquitoes were captured, and the dominant genus was Anopheles (58.6%), followed by Culex (31%), Armigeres (9.9%), Aedes (0.2%), and Lutzia (0.2%). Among 843 mosquitoes collected from Sorong (Aimas), Culex was 98.9%, followed by Aedes (0.8%) and Anopheles (0.2%). The result of nested PCR found two An. sundaicus from Sumba carried Plasmodium which belongs to P. falciparum and P. vivax species (0.14%). Although we did not detect the presence of Plasmodium in mosquitoes other than the genus Anopheles, in this study, we found Anopheles species that have never previously been reported from the area, namely An. bailey and An. barbirostris from Papua and An. nivipes in West Sumba.
Vegetation diversity and structure of urban parks in Cilegon City, Indonesia, and local residents’ perception of its function MUHLISIN MUHLISIN; JOHAN ISKANDAR; BUDHI GUNAWAN; MARTHA FANI CAHYANDITO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220706

Abstract

Abstract. Muhlisin, Iskandar J, Gunawan B, Cahyandito MF. 2021. Vegetation diversity and structure of urban parks in Cilegon City, Indonesia, and local residents’ perception of its function. Biodiversitas 22: 2589-2603. The existence of urban parks is very essential for cities because they provide ecosystem services and benefits to humans. Diversity and structure of vegetation in urban parks play significant role in delivering several functions of ecosystem services. This paper aims to explore and analyze the vegetation diversity and structure, as well as ecosystem services of eight urban parks in Cilegon City, Banten Province, Indonesia. Vegetation analysis and residents’ perception are also conducted in this research. The analysis of vegetation is focused on horizontal structure of vegetation, including plant diversity, plant habitus, the original distribution of the plants, and vertical structure of vegetation (canopy stratification and vegetation profile). The residents’ perceptions are collected using interviews of selected respondents. The identification of vegetation recorded 114 plant species from 46 families are found in the concerned urban parks, however, the diversity and species richness in these parks tended to be low. The vegetation communities are uncertain and dominated primarily by non-native and invasive species. The canopy stratification is relatively homogeneous with the dominant plants having less than one meter in height, such as Ruellia simplex and Syzygium paniculatum. The vegetation planted in the urban parks are mostly ornamental plants that provide much more aesthetic function rather than ecological purpose, daily human needs, or other ecosystem services. This fact shows that urban parks in the study area have only few ecosystem services, hence they need some improvements in order to have more environmental services and to be able to enjoy more by the community.
Diversity of lactic acid bacteria isolated during fermentation of indigenous cassava obtained from Sumba, East Nusa Tenggara, Indonesia IMMANUELLA BERLIANA ELVANI HUTAJULU; PERISKILA DINA KALI KULLA; Endah Retnaningrum
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220703

Abstract

Abstract. Hutajulu IBE, Kulla PDK, Retnaningrum E. 2021. Diversity of lactic acid bacteria isolated during fermentation of indigenous cassava obtained from Sumba, East Nusa Tenggara, Indonesia. Biodiversitas 22: 2561-2570. Traditional fermented food products hold a great significance in the routine diet of Indonesian inhabitants. The present study investigated the diversity of lactic acid bacteria (LAB) found in fermented indigenous cassava, ubi karet busuk, produced in Sumba, East Nusa Tenggara, Indonesia. The isolated LAB was characterized for their phenotypic, biochemical, and molecular traits, which involved 16S rRNA gene sequencing, determination of lactic acid fermentation pathway, and measurement of b-glucosidase and tannase activity in the LAB strains. During fermentation, 15 LAB strains were obtained from the cassava. Among these, 93.33% strains were found to be homofermentative, whereas 6.73% strains displayed heterofermentative traits. BLASTn and phylogenetic analysis classified 15 LAB strains into six species, namely Enterococcus faecium, Enterococcus faecalis, Enterococcus casseliflavus, Leuconostoc mesenteroides, Lactococcus lactis, and Enterococcus sp. Among these, 10 LAB strains displayed b-glucosidase activity, with highest enzyme activity of 7.13 U/mL recorded in L. lactis F14IS3. Interestingly, two of these strains also showed expression of tannase, with F6IS1 strain displaying highest enzyme activity of 38.23 U/mL. Thus, the study results highlighted the suitability of these b-glucosidase and tannase-producing LAB strains to be used as a starter in the fermentation of cassava and other substrates such as vegetables, fruits, and legumes. These strains can also be utilized in animal feed, food, and beverage industries.
Molecular identification of Gracilaria species (Gracilariales, Rhodophyta) obtained from the South Coast of Java Island, Indonesia Maria Dyah Nur Meinita; Nashiatul Akromah; Nuraina Andriyani; Setijanto SETIJANTO; Dicky Harwanto; Tao Liu
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220759

Abstract

Abstract. Meinita MDN, Akromah N, Andriyani N, Setijanto, Harwanto D, Liu T. 2021. Molecular identification of Gracilaria species (Gracilariales, Rhodophyta) obtained from the South Coast of Java Island, Indonesia. Biodiversitas 22: 3046-3056. The study of seaweeds diversity and species identification is an important component of marine resource management. However, seaweed identification based on morphological characteristics has several limitations. Besides, DNA barcodes or the partial sequences of cytochrome c oxidase I (COX1) have been proved to identify seaweeds at the species level. To date, Gracilaria species molecular diversity has not been studied in the South Coast of Java Island. Hence, this study aimed to identify the Gracilaria spp. obtained from 6 different beaches along the South Coast of Java, based on the COX1 gene. COX1 gene utilization for identifying and observing the genetic diversity, both intraspecific (genetic variation within species) and interspecific (variations between species) of Gracilaria species in this study has produced good results. A total of 13 seaweed samples collected from beaches in this study were identified as Gracilaria salicornia, G. edulis, G. firma, and G. textorii. The results of genetic diversity analysis conducted using the COX1 gene showed the intraspecific diversity of G. edulis obtained from the beaches of Kondang Merak, Kukup, Nusakambangan, and Karapyak was included in the moderate diversity category. Also, the intraspecific diversity of G. salicornia obtained from the beaches of Kondang Merak, Kukup, and Nusakambangan was included in the moderate diversity category, while the intraspecific diversity of G. textorii from Menganti and Karapyak Beach had no diversity.
The cytotoxic and apoptotic effects of wild and polyploidy genotype of Artemisia cina extracts on the WiDr colon and HTB-183 lung cancer cell lines Elizabeth Kristiani; Sri Kasmiyati; Maria Herawati
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220735

Abstract

Abstract. Kristiani EBE, Kasmiyati S, Herawati MM. 2021. The cytotoxic and apoptotic effects of wild and polyploidy genotype of Artemisia cina extracts on the WiDr colon and HTB-183 lung cancer cell lines. Biodiversitas 22: 2844-2852. Artemisia belongs to the Asteraceae family, usually used for traditional treatments of various diseases in China. Artemisia cina Berg ex Poljakov, which lots found in Indonesia, has not been studied much like a cancer drug. The research aims were (i) to compare the bioactive content of hexane and ethyl acetate extract of wild (TWN and KJT) and polyploidy type (J and M) of A. cina and its cytotoxicity on WiDr and HTB-183 cancer cells, (ii) to evaluate the cytotoxicity mechanism of the most substantial extract of it. All of the extracts were prepared by maceration methods using hexane and ethyl acetate separately. The research method used was quantitative with experimental design. The determination of quercetin, kaempferol, and artemisinin used HPLC. The cytotoxicity of the extract determined using the MTT method. The assay of the specific protein related to apoptosis using ICC assay. The content of three bioactive compounds on ethyl acetate extracts higher than hexane extract, in which the extract of J and KJT genotypes had higher levels than M and TWN genotypes. The IC50 value (µg/mL) of extracts against cancer cell lines tested was about 400-700 hexane extracts and 200-500 ethyl acetate extracts. The BCl-2 expressions of both cancer cell lines decreased by treating TWN-EA and M-EA, while the P53 expressions increased. The TWN-EA induced apoptosis of HTB-183 cell lines through caspase-8 and caspase-9 pathways, but M-EA induced caspase-9 pathway only. The TWN and M genotypes were potential to use as anticancer agents on colon and lung cancer.
Population dynamics of anchovy pekto (Stolephorus waitei) in the waters of Malacca Strait, North Sumatra, Indonesia Miswar Budi Mulya; ARLEN HANEL JHON; ZULHAM APANDY HARAHAP
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220718

Abstract

Abstract. Mulya MB, Jhon AH, Harahap ZA. 2021. Population dynamics of anchovy pekto (Stolephorus waitei) in the waters of Malacca Strait, North Sumatra, Indonesia. Biodiversitas 22: 2693-2698. Anchovy pekto or Stolephorus waitei (Engraluidae: Clupeiformes) is one of the fish in the eastern coastal part of North Sumatra, occupying the waters of Malacca Strait. This research presents the population dynamics of S. waitei following its age, growth, mortality, and recruitment pattern based on the length-frequency data. Fork length (FL) and total weight (TW) data of S. waitei were collected at three sites from April to September 2018. A total of 1994 specimens with FL from 4.0 to 6.7 cm and TW from 0.2 to 2.3 g with the length-weight relationship of TW = 0.073FL3.0046 (R2=0.765) indicated a near isometric and positive allometric growth in the water region. The relative condition factor (Kn) ranged from 1.01 to 1.02, indicating an estimate of good condition of the fishes. The growth parameters based on von Bertalanffy was 6.56 cm (L?) with the growth coefficient (K) of 0.34 yr-1 and t0 (anchovy age at zero length ) of 0.6506. The total mortality (Z), natural mortality (F), and catch mortality (M) of S. waitei were 0.64, 1.39, and 0.75 yr-1 respectively, which suggest that the fishing activities of S. waitei are still in normal intensity. The recruitment pattern of S. waitei was stable between April and August with its peak in June by 14.21%. The fishing activities may then be limited during the spawning months (May to June) of S. waitei to maintain their population in the Strait of Malacca.
Diversity and abundance of nematodes in soil treated with solarization treatments Astri Harnov Putri; Siwi Indarti; Tri Harjaka
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220708

Abstract

Abstract. Putri A H, Indarti S, Harjaka T. 2021. Diversity and abundance of nematodes in soil treated with solarization treatments. Biodiversitas 22: 2612-2618. Solarization is a tillage cultivation technique that influences the presence of nematodes in agricultural land. This research aims to determine the solarization effect on soil temperature and nematode abundance and diversity in the soil. Observations on species, diversity, and abundance of the nematodes were performed at the Nematology Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Gadjah Mada University. A completely randomized design (CRD) was used with six treatments and three replications. Meanwhile, the treatment groups were based on solarization duration (in days) on the soil which includes A(3), B(7), C(14) D (30), and K0 (control, without solarization) and K1 ( control, farmer treatment). Field observations were performed to measure soil temperature, moisture, and pH. Nematodes were analyzed in the laboratory to determine the genus, abundance, and diversity. Furthermore, extracted plant-parasitic nematodes were isolated by using the whitehead tray method while the entomopathogenic group was isolated using the baiting method. The results showed the differences in tillage treatment and solarization affect soil temperature (diversity and abundance). A polynomial regression model explained the relationship between increased solarization time and soil temperature. The highest soil temperature was found in cultivated land and solarization land for 14 days (41.0oC) and the lowest was in the treatment of farmers (33.67oC). The lowest abundance was found at 14 days solarization, namely 286 individuals/100g, and the diversity index value of 1.37. The highest diversity index was found in the treatment of farmers with an index value of 1.75. The solarization treatment is best applied in the field for 14 days.
Zoonotic cutaneous leishmaniasis and Leishmania infection among Meriones shawi population in Setif Province, Algeria Amina Fellahi; NACER DJIRAR; ABDELKADER CHERIEF; ABDELKRIM BOUDRISSA; NAOUEL EDDAIKRA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220701

Abstract

Abstract. Fellahi A, Djirar N, Cherief A, Boudrissa A, Eddaikra N. 2021. Zoonotic cutaneous leishmaniasis and Leishmania infection among Meriones shawi population in Setif Province, Algeria. Biodiversitas 22: 2547-2554. Zoonotic cutaneous leishmaniasis (ZCL) is the most endemic disease in Algeria. Leishmania major is the causative agent, and Gerbils Meriones shawi and Psammomys obesus are the main reservoir hosts. The Province of Setif in Algeria has become a very active focus for this zoonosis. Our study was carried out from January 2017 until January 2019 in ten representative stations of the study area, with an aim to identify the association between zoonosis and reservoir host species. Eighty-six (86) specimens of M. shawi and three (3) specimens of P. obesus were captured and determined. Superficial lesions were subjected to Giemsa stained smears. In addition, Livers and spleens were tested for Leishmania DNA using ITS1 PCR. The results showed the presence of M. shawi in almost all communities of Setif Province and the highest population with 50 specimens (58.13%) was in the center and south. The microscopic and molecular detection of Leishmania showed high infestation in most specimens of Meriones (32/86) with a rate of 37.20 %, however, no infestation in Psammomys has been detected. The PCA results showed a negative association between rainfall and ZCL cases; however, positive association was found between temperature and the human CL cases. Also, positive association was demonstrated between ZCL in south and center of Setif Province and Meriones captured. Our finding, confirm that M. shawi is the principal reservoir host (76.78%) of the ZCL in Setif Province, since P. obesus was nearly absent (2.67%).
Reptiles in the Pontianak and Kubu Raya Residental Area, West Kalimantan, Indonesia Anandita Eka Setiadi; HANUM MUKTI RAHAYU
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220725

Abstract

Abstract. Setiadi AE, Rahayu HM. 2021. Reptiles in the Pontianak and Kubu Raya Residental Area, West Kalimantan, Indonesia. Biodiversitas 22: 2763-2770. The increase in population is directly proportional to housing needs. Expansion of residential areas can threaten the diversity of reptiles in West Kalimantan. This study aims to inventory and identify the diversity of reptiles in residential areas in Pontianak and Kubu Raya, West Kalimantan province, Indonesia. This research gives a better understanding of reptilian urban species to support monitoring and evaluation of biodiversity in West Kalimantan. This research uses the descriptive exploratory method. Four study areas were selected based on a proportion of impervious surfaces. Data collection used the VES (Visual Encounter Survey). Identification refers to Reptile field guides and identification books. The data were analyzed descriptively qualitatively, the level reptile diversity was analyzed using the Shannon Diversity Index, and the similarities of reptiles between the residential area in Pontianak and Kubu Raya were assessed using the Jaccard similarity coefficient. The results showed, from 50 individual reptiles found in Pontianak and Kubu Raya, there were 13 reptile species from the order Squamata and the order Testudines. The Shannon-Wiener Diversity Index of 2.26 and Jaccard similarity coefficient 0.384, indicating that the level of diversity of reptile species in the two study areas is classified as moderate.

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