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Biodiversitas Journal of Biological Diversity
Published by Universitas Sebelas Maret
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 16 Documents
 1   2 
Search results for , issue "vol. 9 no. 2 (2008)" : 16 Documents clear
Screening of bioantagonistic bacteria for biocontrol agent of Rhizoctonia solani and surfactin producer YULIAR YULIAR
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090201

Abstract

The objective of this research was to screen 31 of bacteria isolates that have potency to control Rhizoctonia solani growth and isolates capability to produce surfactin. R. solani growth inhibition was performed uses paper discs containing a 5 days cultivation of isolates culture. Surfactin activity assay was performed on LB agar medium. Results of the screening showed that the highest growth inhibition was obtained for isolates code 54 (96.43%), KC4 (93.45%), and 163 (93.19%). All of the isolates were cooproducer of surfactin and iturin, and the highest biosurfactan index was obtained for isolate KB2 (3.91).The four potential isolates were identified, as Bacillus pantotheinticus (isolate 54 and isolate 163), Bacillus brevis (isolateKC4), and Bacillus sp(isolate KB2).© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: biocontrol, Bacillus pantotheinticus, Bacillus brevis, Bacillus sp., Rhizoctonia solani, surfactin
Phosphate solubilizing activities of Actinomycetes isolated from Waigeo, Raja Ampat islands, West Papua SRI WIDAWATI; ARIF NURKANTO; I MADE SUDIANA
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090202

Abstract

Actinomycetes is a major microbial group observed in soil, and contributes to nutrient cycling. This study is intended to verify physiologicalcharacters and phosphate solubilizing ability of Actinomycetes isolated from soil of Raja Ampat, West Papua. Most of isolates (RCW16-9,RCW16-8, W5-6, RCW25-1, RCW26-5, W28-4, W3-1, W3-7, W17-7, and W10-1) belonged to Streptomyces genera. The isolates produceclear zone in Pivoskaya after 3 days incubation. The liquid growth of this isolate rapidly utilizes glucose, and after 24 days of incubationalmost 95% glucose was consumed. Decrease of pH from 6.1 to 4.3 may stimulate dissolution of calcium phosphate, and about 21 mg/L-Pwas observed in bulk solution. An increase of phosphomonoesterase activity during incubation is concomitant with the release oforthophosphate into bulk solution. Acidity of cultures increased may stimulate solubilization of calcium phosphate. Most strains producephosphomonoesterase enzyme, indicating that actinomycetes are important soil microbes responsible for mediation and stimulation of bothinorganic and organic phosphate dissolution. Physiological and biomass growth character of phosphate solubilizing actinomycetes could beimportant taxonomic indicator for identifying and grouping soil actinomycetes.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Actinomycetes, phosphate solubilizer, phosphomonoesterase, glucose
The effect of pauh kijang (Irvingia malayana Oliv ex. A. Benn) in decreasing parasitemia percentage in mice infected with Plasmodium berghei PRAPTIWI PRAPTIWI; CHAIRUL CHAIRUL
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090204

Abstract

This study was conducted to determine the influence of pauh kijang (Irvingia malayana Oliv.) stem bark ethanol extract in decreasing parasitemia in mice infected with Plasmodium berghei. Mice were divided into 6 groups as treatments: (1) negative control (CMC), pauh kijang ethanol extract with the concentration of (2)1, (3)10, (4)100, (5) 1000 mg/kg BW, and (6) quinine as positive control. The result showed that meridian effective dose (ED50) of pauh kijang ethanol extract was 36.95 mg/kg BW. Treatment of 100 mg/kg BW lowered parasitemia level by 63.8%, and this treatment did not give significant different (P>0.05) compared to positive control. The result suggests that pauh kijang ethanol extract had antiplasmodial activity since this extract reduces parasitemia more than 30%.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Irvingia malayana, antimalarial, malaria, ED50, plasmodium
Biochemical analysis of extracting fermented coconut oil YATI SUDARYATI SOEKA; JOKO SULISTYO; ELIDAR NAIOLA
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090203

Abstract

Vegetable oil can be produced from a perennial plant such as coconut (Cocos nucifera L.). There are few techniques for coconut oil extraction, such as physical, chemical, and fermentative processes. The fermentation process uses microbial inoculum as starter. Ground coconut meat was soaked in warm water, than squeezed several times to get coconut milk. After being allowed to stand for 4-5 hours, it separated into two layers, cream and skim. Starter was prepared from a mixture of milk and coconut water (1:9, v/v) which enriched with 2% tomato extract, 0.5% urea, and 1.0% molasses and then preincubated for 5 days under agitation. Starter with different concentration (1.0; 2.5; 5.0; and 10%) were added onto coconut milk and allowed to be fermented for over night. The extracting oil was analyzed for further experiment, especially, on its antibacterial activity. The maximum yield of 23% was achieved by using 2.5% starter. Total protein, fat, FFA, and cholesterol content of the fermented coconut oil were 0.05%, 96.45%, 0.29%, and 0.008%, respectively. The gas chromatogram showed that this oil contained high lauric acid (46.20%), and 13.94% miristic, 5.97% palmitic, 9.00% palmitoleic, and 19.73% stearic acid, respectively.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: coconut oil, fermentation, antibacterial activity, biochemistry analysis.
Estimation of genetic variation of Amorphophallus titanum Becc. based on Random Amplified Polymorphic DNA YUYU SURYASARI POERBA; YUZAMMI YUZAMMI
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090206

Abstract

Amorphophallus titanum Becc. (Araceae), is known by titan arum (western) or bunga bangkai (Indonesian), is the bulkiest inflorescence in the world. The species is valued for its unique character of their inflorescence. The species has been overexploited and is considered as vulnerable plant species. The present study is carried out to assess genetic diversity and to estimate genetic relationship among 22 accessions collected from two different populations in Sumatra using Random Amplified Polymorphic DNA (RAPD). Nine RAPD primers generated 143 scorable bands which 137 (95.84%) of them were polymorphic. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic dissimilarity value among accessions was from 0.14 to 0.59, while the range of genetic dissimilarity among populations was from 0.67 and 0.77. These values showed that A. titanum from Sumatra were more genetically diverse among genotypes than that of among populations.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Amorphophallus titanum Becc. random amplified polymorphic DNA.
Cucumber (Cucumis sativus L.) relationship analysis using RAPD-PCR and isozyme methods NUR INDAH JULISANIAH; LILIEK SULISTYOWATI; ARIFIN NOOR SUGIHARTO
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090205

Abstract

RAPD-PCR method and isozyme analysis were used to obtain information of genetic relationship among cucumber varieties. Such information is urgently utilized to support plant breeding program of cucumber. Research was done at the Biotechnology Laboratory of Plant Pest and Diseases, Faculty of Agriculture of Brawijaya University, Malang and Molecular Biology Laboratory, Faculty of Matemathic and Natural Sciences of Brawijaya University, Malang. DNA isolation was done using CTAB method by additional NaCl modification. Sixteen primers from operon were employed to amplify DNA genome by RAPD-PCR. Two enzymes, Esterase and AAT were chosen for isozyme analysis. Clad 97 Program was used for analyzing the data and results in data grouping based on proximity value. Cluster analysis based on isozyme data indicated that there was an adequate lower genetic variation in cucumber, where seven of nine tested varieties showed proximity value of 1.00. Eleven of sixteen primers in RAPD-PCR analysis produced DNA bands. Relativity analysis by using RAPD-PCR method showed high enough of genetic variation. Relativity analysis by using both methods showed that variety 07 was the furthermost. The proximity value between varieties 01 and 02 was 0.916667, these varieties have the higest proximity value among allvarieties.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Cucumis sativus L., RAPD-PCR, isozyme.
Chemical properties of different rattan species traded in Central Sulawesi Province ANDI TANRA TELLU
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090207

Abstract

The aim of this study was to undstand chemical properties of different rattan species traded in Central Sulawesi Province. The chemical of rattan were tested according to SII.1657-85 for wich character of holoselulosa, SII.0443-81 for wich character of -selulosa, SII.0528-81 for wich character of lignin, and SII.1292.85 for wich character of silica. The study indicated that chemical properties of rattan varied amongst the species. The differences are supposed to have important criteria in identifying different species of rattan species traded in Central Sulawesi. Based on the result the highest quality of rattan is Calamus inops and the lowest is Calamus ornatus var. celebicus (lambang).© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: chemical, rattan, traded.
Study on seed germination and seedling growth models of sandalwood (Santalum album L.) of several mother seed trees in Belu Regency, East NusaTenggara ALBERTUS HUSEIN WAWO
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090209

Abstract

Sandalwood (Santalum album L.) is one of the economic plants that are threatened rare. Belu regency is a part of East Nusa Tenggara Province was known as center of natural distribution of sandalwood. In 2005 was found some mother seed trees at several locations in Belu are Suriklulik, Lelowae, Biau, Uabau, Haitimuk, Alas and Tialai. For research propose every location was chosen only one mother seed tree that was marked with capital letters as followed ; A is mother seed tree from Suriklulik, B from Lelowae, C from Biau, D from Uabau, E from Haitimuk, F from Alas and G from Tialai, In this research was studied the germination of sandalwood seeds from severalmother seed trees was mentioned above and some aspects of seedling growth include model of seedling growth and correlation between height and number of leaves growth during 5 months. This research was carried out in paranet house during 5 months and seedling was planted in polybag. Research was designed follow the Completely Randomized Design with 4 replications. The result of this study as follow: The mother seed trees of sandalwood influenced germination process and seedling growth especially on height and number leavesof seedlings. Seedling growth from each mother seed trees follow models of logistic growth with high value of coefficient determination about > 0,83, and be found also, close correlation between growth of seedling height with growth of seedling leaves was marked by high value of coefficient correlation about >0.85.Several mother seed trees was marked with capital letter E, D, C, A and G are better than F and B.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Santalum album L., mother seed trees, germination, seedling growth, Belu.
Starch conversion of ganyong (Canna edulis Ker.) to bioethanol using acid hydrolysis and fermentation LILY SURAYYA EKA PUTRI; DEDE SUKANDAR
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090208

Abstract

Starch of ganyong is one of the sources of ethanol which is able to be produced by acid hydrolysis and fermentation process. It had high concentration of carbohydrate that is 80%, so it could produce glucose highly within acid hydrolysis process. The result showed that the optimal amount of reducing sugar had been produced by nitrate acid 7% (dextrose equivalent, DE = 28.4). Nevertheless, type and concentration of acid had no significantly correlation to reducing sugar yielded. The total amount of glucose had correlation to amount of ethanol, in fermentation process. The optimal amount of ethanol was yielded from 4.81% of glucose and it produced about 4.84% ethanol. The more amount of glucose was yielded the more ethanol was produced. Controlling pH every 12 hours did not affected to production of ethanol significantly.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: bioethanol, Canna edulis Ker, acid hydrolysis, fermentation.
Habitat study of Styphelia abnormis (Sond.) J.J.Smith. in Manitalu Hill, East Waigeo Nature Reserve, Waigeo Island, West Papua DEDEN MUDIANA
Biodiversitas Journal of Biological Diversity Vol. 9 No. 2 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090211

Abstract

Styphelia abnormis (Sond.) J.J. Smith is a member of family Epacridaceae that grows in East Waigeo Nature Reserve (Cagar Alam Waigeo Timur). An inventory was done using 30 sampling plots of 2 x 2 m2. The results were: density 2.375 per m2 and presence 90%. This species grew as a shrub on ultramafic soil in open hilly areas at 50-80 above sea level (a sl). Styphelia abnormis is a plant species that can grow on ultramafic soil in East Waigeo NR.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Styphelia abnormis, habitat, East Waigeo Nature Reserve (Cagar Alam Waigeo Timur).

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