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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
Other
Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 8 Documents
 1 
Search results for , issue "vol. 2 no. 1 (2010)" : 8 Documents clear
Ripening for improving the quality of inoculated cheese Rhizopus oryzae SOLIKAH ANA ESTIKOMAH; SUTARNO SUTARNO; ARTINI PANGASTUTI
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020101

Abstract

Abstract. Estikomah SA, Sutarno, Pangastuti A 2010. Ripening for improving the quality of inoculated cheese Rhizopus oryzae. Nusantara Bioscience 2: 1-6. Cheese is dairy product resulted from fermented milk in which the fermentation process can be done by lactic acid bacteria or fungus. Rhizopus oryzae is able to produce lactic acid, protease, and lipase. The ripening process changes the taste and texture. The purpose of this study is ripening to improve the quality of inoculated cheese R. oryzae. In this research, the ripening was conducted the concentration variation of temperature (5oC, 10oC, 15oC), and time (7 days, 14 days). The procedure of research consisted of two steps, namely un-ripened cheese preparation followed by ripening cheese preparation. Cheese produced in this study analyzed the value of pH, fat content, protein content, amino acid levels and identification of microbe with ANOVA then followed by DMRT at 5% level of significance. Data results were analyzed with the like’s nonparametric statistical test, followed by Fridman Wilcoxon Signed Rank Test (WSRT) at 5% level significance. The results showed that the preferred ripened cheese panelist was at a temperature of 15oC for 14 days. Ripening conditions affect pH, fat content, protein content and do not affect the levels of amino acids that formed ripened cheese. The best quality ripened cheese, i.e. at a temperature of 15°C for 14 days, had a pH value of 4.40, the highest protein content of 9.78%, and fat content of 35.02%. The results of identified microbe in un-ripened cheese and ripened cheese include Enterococcus hirae (Enterococcus faecalis), Bacillus subtilis, and Aspergillus sp.
Exposure copper heavy metal (Cu) on freshwater mussel (Anodonta woodiana) and its relation to Cu and protein content in the body shell AHMAD INTAN KURNIA; EDI PURWANTO; EDWI MAHAJOENO
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020108

Abstract

Abstract. Kurnia AI, Purwanto E, Mahajoeno E. 2010. Exposure copper heavy metal (Cu) on freshwater mussel (Anodonta woodiana) and its relation to Cu and protein content in the body shell. Nusantara Bioscience 2: 48-53. To determine the relationship of Cu exposure in water to the freshwater mussel exposure experiment is conducted with water containing Cu. Which measured the influence of Cu and protein content in the body shell. This study used the freshwater mussel species, Anodonta woodiana. Oysters were exposed for four weeks in the water with Cu concentration of 0.02 ppm, 0.04 ppm, 0.06 ppm, and 0.00 ppm control. Cu content and protein content in the body shells are checked every week. Cu analysis was done by Atomic absorption spectroscopy (AAS) method and the protein content using Kjeldahl method. Cu analysis showed elevated levels of Cu in mussel body after exposure. The pattern of increase in Cu content was not the same, where the pattern of the largest increases occurred after the fourth week. The statistical test showed no significant effect between the treatment with Cu accumulation in the body shell. Protein analysis showed an increase of protein content after exposure of the second week and decreased after the third and fourth weeks. The pattern of changes in protein content varied among the various treatments. The statistical test showed no significant effect between treatment with the protein changes in the body shell. Correlation test of the relationship between concentration of Cu in mussel body protein level showed a positive correlation between them with a fairly good level of relationship (correlation coefficient r = 0.836).
Comparasion of iles-iles and cassava tubers as a Saccharomyces cerevisiae substrate fermentation for bioethanol production KUSMIYATI KUSMIYATI
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020102

Abstract

Abstract. Kusmiyati. 2010. Comparison of iles-iles and cassava tubers as a Saccharomyces cerevisiae substrate fermentation for bioethanol production. Nusantara Bioscience 2: 7-13. The production of bioethanol increases rapidly because it is renewable energy that can be used to solve energy crisis caused by the depleting of fossil oil. The large scale production bioethanol in industry generally uses feedstock such as sugarcane, corn, and cassava that are also required as food resources. Therefore, many studies on the bioethanol process concerned with the use of raw materials that were not competing with food supply. One of the alternative feedstock able to utilize for bioethanol production is the starchy material that available locally namely iles-iles (Amorphophallus muelleri Blum). The content of carbohydrate in the iles-iles tubers is around 71.12 % which is slightly lower as compared to cassava tuber (83,47%). The effect of various starting material, starch concentration, pH, fermentation time were studied. The conversion of starchy material to ethanol has three steps, liquefaction and saccharification were conducted using ?-amylase and amyloglucosidase then fermentation by yeast S.cerevisiaie. The highest bioethanol was obtained at following variables starch:water ratio=1:4 ;liquefaction with 0.40 mL ?-amylase (4h); saccharification with 0.40 mL amyloglucosidase (40h); fermentation with 10 mL S.cerevisiae (72h) producing bioethanol 69,81 g/L from cassava while 53,49 g/L from iles-iles tuber. At the optimum condition, total sugar produced was 33,431 g/L from cassava while 16,175 g/L from iles-iles tuber. The effect of pH revealed that the best ethanol produced was obtained at pH 5.5 during fermentation occurred for both cassava and iles-iles tubers. From the results studied shows that iles-iles tuber is promising feedstock because it is producing bioethanol almost similarly compared to cassava.
Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java TRIBADI TRIBADI; SURANTO SURANTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020103

Abstract

Abstract. Tribadi, Suranto, Sajidan. 2009. Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java. Nusantara Bioscience 2: 14-22. This research is intended to find out the morphological and anatomical variation as well as the protein band pattern of cassava (Manihot esculenta Crantz) widely spread in three different areas of height. The sample collecting is done using simple random sampling in the three different areas of height that is 50, 300, 1000 meters asl in Ngawi District, East Java while the analysis of protein band pattern is done using SDS-PAGE. The result of the research of morphology and anatomy is analyzed descriptively and presented in the form of tables, histograms, and figures. The analysis of protein band pattern is done using quantitative and qualitative analysis that is based on the appearance or not the gel band pattern by counting the molecular weights based on code marker S 8445 and qualitative method based on the quality of the band formed. The band pattern formed is istimated and presented in the form of zimogram. The result of the research shows that the height of the cultivating site very much influences toward variations of root, stem and leaf morphology. The longest root is at 50-meter heights asl (Cabak makao local variety, the widest stem diameter is at 50 meters asl (Cabak makao local variety) the longest leaf and branch is at 300 meters asl (Cabak makao local variety) and 1000 meters asl (Cabak makao local variety). There is no difference of anatomy in the root, stem, and leaf and no difference of protein band pattern either in Adira1 or Cabak makao local variety.
Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics ALFIN WIDIASTUTI; SOBIR SOBIR; MUH RAHMAD SUHARTANTO
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020104

Abstract

Abstract. Widiastuti A, Sobir, Suhartanto MR. 2010. Diversity analysis of mangosteen (Garcinia mangostana L.) irradiated by gamma-ray based on morphological and anatomical characteristics. Nusantara Bioscience 2: 23-33. The aim of this research was to increase genetic variability of mangosteen (Garcinia mangostana L.) irradiated by gamma rays dosage of 0 Gy, 20 Gy, 25 Gy, 30 Gy,35 Gy, and 40 Gy. Plant materials used were seeds collected from Cengal Sub-village, Karancak Village, Leuwiliang Sub-district, Bogor District, West Java. Data was generated from morphological and anatomical characteristics. The result indicated that increasing gamma-ray dosage had inhibited ability of seed to grow, which needed longer time and decreased seed viability. Morphologically, it also decreased plant height, stem diameter, leaf seizure, and amount of leaf. Anatomically, stomatal density had positive correlation with plant height by correlation was 90% and 74%. Gamma rays irradiation successfully increase morphological variability until 30%. Seed cleavage after irradiation increased variability and survival rate of mangosteen.
First record of two hard coral species (Faviidae and Siderastreidae) from Qeshm Island (Persian Gulf, Iran) MAHDI MORADI; EHSAN KAMRANI; MOHAMMAD R. SHOKRI; MOHAMMAD SHARIF RANJBAR; MAJID ASKARI HESNI
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020105

Abstract

Abstract. Moradi M, Kamrani E, Shokri MR, Ranjbar MS, Hesni MA (2009) First record of two hard coral species (Faviidae and Siderastreidae) from Qeshm Island (Persian Gulf, Iran). Nusantara Bioscience 2: 34-37. Two species of hard corals including Cyphastrea chalcidicum (Forskal 1775) (Faviidae) and Coscinaraea monile (Forskal 1775) (Siderastreidae) were collected from the south of Qeshm Island (Persian Gulf, Iran) in the late of 2008. These species were previously reported from southern Persian Gulf, Gulf of Aden, Southeast Africa, and Indo-Pacific. The literature review on the distribution of these two species revealed that these species were firstly recorded from the Persian Gulf. These findings further emphasize the high diversity of coral fauna in the Iranian waters of the northern Persian Gulf.
Isolation and identification of lactic acid bacteria from abalone (Haliotis asinina) as a potential candidate of probiotic SARKONO SARKONO; FATURRAHMAN FATURRAHMAN; YAYAN SOFYAN
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020106

Abstract

Abstract. Sarkono, Faturrahman, Sofyan Y. 2010. Isolation and identification of lactic acid bacteria from abalone (Haliotis asinina) as a potential candidate of probiotic. Nusantara Bioscience 2: 38-42. The purpose of this study was to isolate, select and characterize lactic acid bacteria (LAB) from abalone as a potential candidate probiotic in abalone cultivation system. Selective isolation of LAB performed using de Man Rogosa Sharpe medium. LAB isolate that potential as probiotics were screened. Selection was based on its ability to suppress the growth of pathogenic bacteria, bacterial resistance to acidic conditions and bacterial resistance to bile salts (bile). Further characterization and identification conducted to determine the species. The results showed that two of the ten isolates potential to be developed as probiotic bacteria that have the ability to inhibit several pathogenic bacteria such as Escherichia coli, Bacillus cereus dan Staphylococcus aureus, able to grow at acidic condition and bile tolerance during the incubation for 24 hours. Based on the API test kit, both isolate identified as members of the species Lactobacillus paracasei ssp. paracasei.
Productivity of sugarcane plants of ratooning with fertilizing treatment A. SUTOWO LATIEF; RIZAL SYARIEF; BAMBANG PRAMUDYA; MUHADIONO MUHADIONO
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020107

Abstract

Abstract. Latief AS, Syarief R, Pramudya B, Muhadiono. 2010. Productivity of sugarcane plants of ratooning with various fertilizing treatments. Nusantara Bioscience 2: 43-47. This research aims to determine the sugarcane plants of ratooning productivity with low external input of fertilization treatment towards farmers can increase profits. The method used is the Completely Randomized Block Design (CRBD) with four treatments and three repetitions (4x3). Sugarcane varieties R 579 planted in each patch experiment 5x5 m2. Dosage of fertilizer: P0 = 3.6 kg/year plot experiment was 100% dosage usage of chemical fertilizers used by farmers. Further dosages were P1 (75%) = 2.7 kg/plot, P2 (50%) = 1.8 kg/plot and P3 (0.25%) = 0.9 kg/plot, each supplemented with fertilizer 5 mL of liquid organic/patch a year. Sugarcane crops with a variety of treatment showed no significant difference. The highest productivity was achieved at dosages of P2 (50% chemical fertilizers plus organic fertilizer) is 21.67 kg per square meter. Chemical fertilizers can be saved 7 quintals per hectare a year or Rp 997,500 per year. Additional costs of liquid organic fertilizer Rp. 100,000 per hectare year and labor Rp 100,000 per hectare, so the additional advantage of saving farmers fertilizer Rp. 797,500 per year.

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