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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 7 Documents
 1 
Search results for , issue "vol. 2 no. 3 (2010)" : 7 Documents clear
Serum lipid profile and retinol in rats fed micronutrient rich edible vegetable oil blend HAMID NAWAZ KHAN; HUMAIRA FAROOQI; SHAKIR ALI; JAFAR S. KHAN
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020301

Abstract

Abstract. Khan HN, Farooqi H, Ali S, Khan JS. 2010. Serum lipid profile and retinol in rats fed micronutrient-rich edible vegetable oil blend. Nusantara Bioscience 2: 109-116. The animal rats were given 10% oil mixed in fat-free diet for one month or six months. In the experiment, the groups of rats were fed with the micronutrient (MN) rich blends mixed previously with 1% cholesterol, and their effects were tested on serum lipid profile. Most significant changes in the High Density Lipoprotein (HDL) cholesterol were observed in one-month study where HDL increased from 24 mg/dl in group to 64 mg/dl in the Mustard palm olein oil blend (MP); in mustard oil (MO) alone fed rats, the HDL was 36 mg/dl. Serum retinol was analyzed as one of the important MN in rats receiving the diet mixed with the blend for various duration of time. The results assume great significance as MO or palm olein oil (PO) alone could not bring the maximum beneficial effects, and the blends appear to have more merit as healthy oils in alleviating adverse health condition such as coronary heart disease (CHD), diabetes, obesity, and hypertension.
Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus) LINTAL MUNA; OKID PARAMA ASTIRIN; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020304

Abstract

Abstract. Muna L, Astirin OP, Sugiyarto. 2010. Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus). Nusantara Bioscience 2: 126-134. This experiment was performed to examine the effect of Pandanus conoideus Lam. var. yellow fruit extract on the percentage of the living fetus, the death intrauterus, heavy and long of fetus, fetus morphology, and skeleton structure of fetus. The experiment was used by 25 pregnant mice that randomly were divided into 5 groups, they contained 5 mice. Each group was given with a different dose. The P1 group (control) was given 1 mL sesame oil, the group P2, P3, P4 and P5 were respectively given the yellow fruit extract 0,02 mL, 0,04 mL, 0,08 mL and 0,16 mL. The P. conoideus var. yellow fruit extract was given orally on day 5 to 17 of gestation (organogenesis period). Observation was carried out on day 18 of gestation by cesarean section to take the fetus from the uterus. Fetus morphology was observed after taking fetus from uterus, whereas observation of skeleton structure was made wholemount preparation with dual colorization, they are Alcian blue and Alizarin Red-S. The result was analyzed with one way ANOVA. Results showed that giving yellow fruit extract didn't influence to the percentage of the living fetus, the death intrauterus, heavy and long of fetus. The effect of giving yellow fruit extract to the maternal was abnormality skeleton (lordosis) of fetus in the dose of 0.16 mL and obstacles to the ossification of fetus.
Expression of vimentin protein and neurofilament on forelimb buds of black-6 mice on gestation day 12 induced by 2-methoxyethanol by RT-PCR YULIA IRNIDAYANTI
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020302

Abstract

Abstract. Irnidayanti Y. 2010. Expression of vimentin protein and neurofilament on forelimb buds of black-6 mice on gestation day 12 induced by 2-methoxyethanol by Real-Time RT-PCR. Nusantara Bioscience 2: 116-120. The aim of this study was to investigate impact of 2-methoxyethanol, a major industrial chemical of plastic. Gene expression analysis is increasingly important in biological research, while real-time reverse transcription PCR (RT-PCR) is becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Pregnant black-6 mice were injected intraperitoneally to 7.5 mmol/kg of 2- methoxyethanol on gestation day (GD) 10. Embryo was obtained on gestation day 12. Forelimb buds of embryo were collected and then put in the tube, which containing RNA-latter solution. To identify gene expression changes in forelimb bud caused induction 2-methoxyethanol, Real-Time PCR was used in this research. For the experiments, the real-time RT-PCR Light Cycler technology was used. The results suggested that injection of 2- methoxyethanol, in prenatal period especially on gestation day 12, the expression of vimentin in forelimb buds of mice treatment increase than control mice. Meanwhile, the expression of neurofilament tended to decrease, indirectly is not caused by the injection of 2- methoxyethanol.
Induced mutations by gamma ray irradiation to Argomulyo soybean (Glycine max) variety DIANA SOFIA HANAFIAH; TRIKOESOEMONINGTYAS TRIKOESOEMONINGTYAS; SUDIRMAN YAHYA; DESTA WIRNAS
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020303

Abstract

Abstract. Hanafiah DS, Trikoesoemaningtyas, Yahya S, Wirnas D. 2010. Induced mutations by gamma-ray irradiation to Argomulyo soybean (Glycine max) variety. Nusantara Bioscience 2: 121-125. Induced mutation by gamma-ray irradiation is one way to increase genetic variability of plants. This research used gamma-ray irradiation on low doses (micro mutation). The aim of this research was to know the response of doses level by micro mutation on gamma-ray irridation to the growing and development of Argomulyo variety of soybean [Glycine max (L) Merr]. The seeds were irradiated by gamma-ray micro mutation doses, namely 0 gray, 50 gray, 100 gray, 150 gray, and 200 gray. Variations that were obtained of each character at generation M1 and M2 influences plants growth and development either through qualitative and quantitative that finally will influence plant’s production. The average highest genetic variation at M2 generation of soybean was on 200 Gray doses. Results of the research indicated that gamma-ray irradiation on 200 Gray doses effectively caused by plant variation genetic.
Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae YAYAN SANJAYA; DADANG MACHMUDIN; NANIN DIAH KURNIAWATI
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020305

Abstract

Abstract. Sanjaya Y, Machmudin D, Kurniawati ND. 2010. Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae. Nusantara Bioscience 2: 135-140. The effect of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura Fab. larvae has been carried out. The method was used Probit analysis, and based on LD 50 the virus was infected to know body weight and post-infection damage.The damage of histological structure caused by SlNPV (0, 315, 390, 465, 540 dan 615 PIB/mL) was investigated after 0, 12, 24, 72 and 96 hours post infection. The histological material was prepared by using paraffin method after fixation with Bouin Solution, then slice into 7 um and colored with Hematoxylin-Eosin. The result showed that the exposure SlNPV cause decreasing food consumption especially on 540 PIB/mL give average rate as amount of 0.1675 mg. The descriptive observation on structural intact of peritrophic membrane histology caused by SlNPV infection shows a tendency to decrease, while in control, there was no damage at all. The longer the exposition of virion in the midgut lumen the more damage on peritrophic membrane occurred. The severest damage occurred 96 hours after infection. The result proves that haNPV virion can destroy histological structure of midgut.
Hitherto unreported Agaricus species of Central India ALKA KARWA; MAHENDRA K. RAI
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020306

Abstract

Abstract. Karwa A, Rai MK. 2010. Hitherto unreported Agaricus species of Central India. Nusantara Bioscience 2: 141-145. Melghat forest region from Central India was surveyed for occurrence of medicinal and culinary mushrooms during the years 2005-2008. Out of total 153 species, ten species of Agaricus were recorded from different localities. Of these, seven species namely Agaricus bitorquis, A. subrufescens, A. augustus, A. placomyces, A. essettei, A. basioanolosus and Agaricus sp. nov (a new species) are being reported for the first time from the region. The commercial button mushroom Agaricus bisporus lacks good breeding characters due to its bisporic nature. These wild cousins of the button mushroom can definitely prove to be a good source of genetic manipulations to the existing strains and also to develop new strains with improved characters.
Diversity of secondary metabolites from Genus Artocarpus (Moraceae) ALIEFMAN HAKIM
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020307

Abstract

Abstract. Hakim A. 2010. The diversity of secondary metabolites from Genus Artocarpus (Moraceae). Nusantara Bioscience 2:146-156. Several species of the Artocarpus genus (Moraceae) have been investigated their natural product. The secondary metabolites successfully being isolated from Artocarpus genus consist of terpenoid, flavonoids, stilbenoid, arylbenzofuran, neolignan, and adduct Diels-Alder. Flavonoid group represents the compound which is the most found from Artocarpus plant. The flavonoids compound which is successfully isolated from Artocarpus plant consists of the varied frameworks like chalcone, flavanone, flavan-3-ol, simple flavone, prenylflavone, oxepinoflavone, pyranoflavone, dihydrobenzoxanthone, furanodihydrobenzoxanthone, pyranodihydrobenzoxanthone, quinonoxanthone, cyclopentenoxanthone, xanthonolide, dihydroxanthone.   

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