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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 7 Documents
 1 
Search results for , issue "vol. 3 no. 1 (2011)" : 7 Documents clear
Optimization of DNA extraction of physic nut (Jatropha curcas) by selecting the appropriate leaf EDI PRAYITNO; EINSTIVINA NURYANDANI
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030101

Abstract

Abstract. Prayitno E, Nuryandani E. 2011. Optimization of DNA extraction of physic nut (Jatropha curcas) by selecting the appropriate leaf. Nusantara Bioscience 3: 1-6. Jatropha curcas L. has important roles as renewable source of bioenergy. The problem occurs on difficult of DNA extraction for its molecular breeding programs. The objectives of this research were to study which leaf best as source of DNA extraction. Four accessions were used, namely J1 and J2 (Jawa Tengah), S1 (South Sumatra), and S2 (Bengkulu). First, third, fifth, seventh, and yellow leaves for each accession were extracted using modification of Doyle and Doyle (1987) method. Visualization and comparison with Lambda DNA, Spectrophotometer UV-Vis and cutting DNA with EcoRI enzyme were show quality and quantity of DNA. The result showed that third leaves have sufficient quality and quantity as source of DNA. Third leaves DNA quantity for J1 (19.33 µg/mL), J2 (26.21 µg/mL), S1 (31.20 µg/mL), and S2 (61.03 µg/mL), and quality for each accession were 1.9063 (J1), 2.0162 (J2), 2.0116 (S1), and 2.0856 (S2).
Characterisation of taro (Colocasia esculenta) based on morphological and isozymic patterns markers TRIMANTO TRIMANTO; SAJIDAN SAJIDAN; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030102

Abstract

Abstract. Trimanto, Sajidan, Sugiyarto. 2011. Characterization of taro (Colocasia esculenta) based on morphological and isozymic patterns markers. Nusantara Bioscience: 7-14. The aims of this research were to find out: (i) the variety of Colocasia esculenta based on the morphological characteristics; (ii) the variety of C. esculenta based on the isozymic banding pattern; and (iii) the correlation of genetic distance based on the morphological characteristics and isozymic banding pattern. Survey research conducted in the Karanganyar district, which include high, medium and low altitude. The sample was taken using random purposive sampling technique, including 9 sampling points. The morphological data was elaborated descriptively and then made dendrogram. The data on isozymic banding pattern was analyzed quantitatively based on the presence or absence of bands appeared on the gel, and then made dendrogram. The correlation based on the morphological characteristics and isozymic banding pattern were analyzed based on the product-moment correlation coefficient with goodness of fit criterion. The result showed : (i) in Karanganyar was founded 10 variety of C. esculenta; (ii) morphological characteristics are not affected by altitude; (iii) isozymic banding pattern of peroxides forms 14 banding patterns, esterase forms 11 banding patterns and shikimic dehydrogenase forms 15 banding patterns; (iv) the correlation of morphological data and the isozymic banding pattern of peroxidase has good correlation (0.893542288) while esterase and shikimic dehydrogenase isozymes have very good correlation (0.917557716 and 0.9121985446); (v) isozymic banding pattern of data supports the morphological character data.
Study on floristic and plant species diversity in the Lebanon oak (Quercus libani) site, Chenareh, Marivan, Kordestan Province, western Iran HASSAN POURBABAEI; SHIVA Z. NAVGRAN
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030103

Abstract

Pourbabaei H, Navgran SZ. 2011. Study on floristic and plant species diversity of the Lebanon oak site (Quercus libani) inChenareh, Marivan, Kordestan Province, western Iran. Nusantara Bioscience 3: 15-22. In order to study floristic and plant species diversity, approximately 450 ha of oak forests were selected in Chenareh, Marivan of Kordestan province in western Iran. Inventory was selectively carried out in 50 m elevation range in the different aspects. Vegetation was surveyed in the four layers including: tree (dbh>5 cm), regeneration (dbh <5 cm), shrub and herb. Diversity and richness indices were used to analyze data in the different vegetation layers. Results indicated that 82 plant species found in the studied site, comprise of 9 tree, 3 shrub and 70 herbaceous. The mean diversities and richness measures were found to be the highest in southwestern and lowest in southeastern and northern aspects for the tree layer. Whereas for the regeneration layer, the mean diversity measures were found the highest in northeastern (i.e., 1-D and H′) and southern (i.e., N2 and N1) and lowest in southwestern (i.e., 1-D, H′ and N1) and southeastern (i.e., N). The mean diversities were found the highest in northern (i.e. N2 and H′) and northwestern (i.e. 1-D and N1 2) and lowest in northeastern aspect in the shrub layer. The mean diversities were also found the highest in western and lowest in northeastern aspect in the herbaceous layer. Moreover, Mean richness and diversity were found the highest in 1500 m asl and lowest in 1750 and 1800 m asl in the tree and shrub layers. Mean richness and diversity were found the highest in 1500 m asl and lowest in 1750 m asl in the regeneration layer. Also, the mean diversities were found the highest in elevation 1700 m asl and lowest in elevation 1800 m asl in the herbaceous layer.
Evaluation structural diversity of Carpinus betulus stand in Golestan Province, North of Iran VAHAB SOHRABI; RAMIN RAHMANI; SHAHROKH JABBARI; HADI MOAYERI
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030104

Abstract

Abstract. Sohrabi V, Rahmani R, Jabbari S, Moayeri H. 2011. Evaluation structural diversity of Carpinus betulus stands in Golestan Province, Northern Iran. Nusantara Bioscience 3: 23-27. In order to investigate structural diversity of Carpinus betulus type in Golestan province, 30 modified Whittaker plots by systematic random system were located. Per plot, the characteristic of trees and shrubs species (Species name, diameter, and height of trees) are recorded. The heterogeneity indices of Simpson, Shannon–Wiener, Simpson’s reciprocal and number of equally common species were used for the quantitative data. Toward better understand from diversity condition in horizontal and vertical composition of stand, the diameter divided in 10 cm classes and Method of Mohajer and the height divided in 10 m height classes and dominant height, then number of diversity of each class extracted by Ecological Methodology software V.7. The results showed with increase of diameter and height classes, decrease species diversity. Also regeneration layers diversity has significant difference with trees layers. Thus, the study of biodiversity changes in different diameter and height category cause ecologically precise perspective in management of forest stands.   
Microanatomy alteration of gills and kidneys in freshwater mussel (Anodonta woodiana) due to cadmium exposure FUAD FITRIAWAN; SUTARNO SUTARNO; SUNARTO SUNARTO
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030105

Abstract

Abstract. Fitriawan F, Sutarno, Sunarto. 2011. Microanatomy alteration of gills and kidneys in freshwater mussel (Anodonta woodiana) due to cadmium exposure. Nusantara Bioscience 3: 28-35. The purpose of this study was to determine the level of Cd accumulation in the gills and kidneys, to know the changes in microanatomic structure of A. woodiana after the various treatments of heavy metals. Completely randomized design pattern of 5 x 3 as used in this laboratory experiment. The amount of exposure of heavy metals Cd was (0 ppm, 0.5 ppm, 1 ppm, 5 ppm, 10 ppm), while the variation of length of exposure time to Cd were (7 days, 14 days, and 30 days). The parameters of Cd accumulation in the gills and kidney was analyzed by using AAS method, while abnormalities of gills and kidney were detected by microanatomy structure. Data collected were then analyzed using the analysis of variance (ANOVA) and continued with further test the DMRT. The results indicated that there is a significant effect in 475.3 > 0.000 and 60150.3 >0.000 with 5% significance level (P<0.05) of Cd treatment on gill and kidney microanatomy of A. woodiana. The changes in microanatomy structure of those organs are including edema, hyperplasia, fusion of lamella, necrosis, and atrophy.
Site suitability to tourist use or management programs South Marsa Alam, Red Sea, Egypt MOHAMMED S. A. AMMAR; MOHAMMAD HASSANEIN; HASHEM ABBAS MADKOUR; AMRO A. ABD-ELGAWAD
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030106

Abstract

Ammar MSA, Hassanein M, Madkour HA, Abd-Elgawad AE. 2011. Site suitability to tourist use or management programs South Marsa Alam, Red Sea, Egypt. Nusantara Bioscience 3: 36-43. Twenty sites in the southern Egyptian Red Sea (Marsa Alam-Ras Banas sector) were surveyed principally for sensitivity significance throughout the periode 2002-2003. Sensitivity of the study area was derived from internationally known criteria, the key words of each criterion and a brief description of its use was described. The present study assigned for the first time a numerical total environmental significance score that gives a full sensitivity significance evaluation for any site to decide to select either for tourist use or management purposes. However, the results of the study still have the availability to arrange sites with respect to one criterion or only two or many of the used criteria whichever needed. Sites selected for protection are categorized as belonging to the following protected area categories: sites 7, 10 (category vi), site 18 (category ib), site 5 (category iv), sites 16, 17 (category ii). Sites selected for tourist uses are suggested to be classified into 2 categories: first category sites (sites 1, 3, 8, 11, 13, 15) which are recommended as tourist use sites with management of the sensitive resources beside non consumptive recreational activities like swimming, diving, boating, surfing, wind-surfing, jet skiing, bird watching, snorkelling, etc.; second category sites (sites 2, 4, 6, 9, 12, 14, 19, 20) which are recommended as tourist use sites with both non consumptive and managed consumptive recreational activities like fishing.
Review: Natural products from Genus Selaginella (Selaginellaceae) AHMAD DWI SETYAWAN
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030107

Abstract

Abstract. Setyawan AD. 2011. Natural products from Genus Selaginella (Selaginellaceae). Nusantara Bioscience 3: 44-58. Selaginella is a potent medicinal-stuff, which contains diverse of natural products such as alkaloid, phenolic (flavonoid), and terpenoid. This species is traditionally used to cure several diseases, especially for wound, after childbirth, and menstrual disorder. Biflavonoid, a dimeric form of flavonoids, is the most valuable natural products of Selaginella, which constituted at least 13 compounds, namely amentoflavone, 2',8''-biapigenin, delicaflavone, ginkgetin, heveaflavone, hinokiflavone, isocryptomerin, kayaflavone, ochnaflavone, podocarpusflavone A, robustaflavone, sumaflavone, and taiwaniaflavone. Ecologically, plants use biflavonoid to response environmental condition such as defense against pests, diseases, herbivory, and competitions; while human medically use biflavonoid especially for antioxidant, anti-inflammatory, and anti-carcinogenic. Selaginella also contains valuable disaccharide, namely trehalose that has long been known for protecting from desiccation and allows surviving severe environmental stress. The compound has very prospected as molecular stabilizer in the industries based bioresources.

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