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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 7 Documents
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Search results for , issue "vol. 3 no. 3 (2011)" : 7 Documents clear
Development of an efficient protocol for genomic DNA extraction from mango (Mangifera indica) DILRUBA ASHRAFUN N. MAJUMDER; LUTFUL HASSAN; MOHAMMAD A. RAHIM; MOHAMMAD A. KABIR
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030301

Abstract

Abstract. Majumder DAN, Hassan L, Rahim MA, Kabir MA. 2011. Development of an efficient protocol for genomic DNA extraction from mango (Mangifera indica). Nusantara Bioscience 3: 105-111. A simple and efficient method for genomic DNA extraction from woody fruit crops containing high polysaccharide levels has been described here. In the present study, three kinds of plant DNA extraction protocols were studied and the target was to establish the water-saturated ether (WSE) with 1.25 M NaCl method as the most efficient protocol for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops. This method involves the modified CTAB or SDS procedure employing a purification step to remove polysaccharides using the WSE method. Precipitation with an equal volume of isopropanol caused a DNA pellet to form. After being washed with 70% ethyl alcohol, the pellet became easily dissolved in TE buffer. Using these three methods, DNA was extracted from samples of 60 mango genotypes, including young, mature, old, frosted old and withered old leaves. Compared with the three studied DNA extraction protocols of mango, it was found that the WSE method with NaCl had the highest value of average percentage (85.44%) in DNA content of the mango genotypes. The average yield of DNA ranged from 5.05 µg/µL to11.28 µg/µL. DNA was suitable for PCR and RAPD analyses and long-term storage for further use.
Synthesis and study of cholosubstituted 4-aroyl pyrazolines and isoxazolines and their effects on inorganic ions in blood serum in albino rats AMOL D. BHOYAR; GANESH N. VANKHADE; PRITHVISIGH R. RAJPUT
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030302

Abstract

Abstract. Bhoyar AD, Vankhade GN, Rajput PR. 2011. Synthesis and study of cholosubstituted 4-aroyl pyrazolines and isoxazolines and their effects on inorganic ions in blood serum in albino rats. Nusantara Bioscience 3: 118-123. Condensation of 2-substituted 3,5-dichloroacetophenone (2a-b) obtained from the condensation of 2-hydroxy 3,5-dichloro-acetophenone (1) and benzoyl chloride were dissolved in NaOH, on treatment under Baker-Venkatraman transformation in presence of KOH with pyridine gives 1-(2-hydroxy-3,5-dichlorophenyl)-3-substituted-1,3-propanediones (3a-b). Then converted into 3-aroyl-6,8-dichloroflavanones (4a-d) by using different aromatic aldehyde in ethanol containing little piperidine. The condensation of (4a-d) and phenylhydrazine hydrochloride, piperidine in DMF gives 3-(2-hydroxy3,5-dichlorophenyl)-4-substitution-1-phenyl-D2pyrazolines (5a-d) and condensation of (4a-d) and hydroxylamine-hydrochloride gives 3-(2-hydroxy-3,5-dichlorophenyl)-4-aroyl-5-substituted isoxazolines (6a-d). The above compounds are screened for their activities and have been found to exhibit significant effects on inorganic ions in blood serum in albino rats.
Selection of parent trees for Rubber (Hevea brasiliensis) breeding based on RAPD analysis FETRINA OKTAVIA; MUDJI LASMININGSIH; KUSWANHADI KUSWANHADI
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030303

Abstract

Abstract. Oktavia F, Lasminingsih M, Kuswanhadi. 2011. Selection of parent trees for Rubber (Hevea brasiliensis) breeding based on RAPD analysis. Nusantara Bioscience 3: 124-129. The parent trees’ clones usually originate from the previous generation having the potential of high production with better agronomical characters. Although, phenotype characters can determine the genetic variability among accessions, they are highly sensitive to environmental factors, so it is often difficult to identify the difference between closely related clones. The genetic variability or phylogenetic relationships among rubber clones can be analyzed using RAPD method, and based on the result, the parent trees can be selected. This research was aimed to analyze the genetic distance among rubber clones using RAPD method. Analysis was conducted on 45 rubber clones with 12 random primers. Pair-wise comparisons of unique and shared polymorphic amplification products were used to generate similarity coefficients. These coefficients were employed to construct a dendrogram by using an Unweighted Pair-Group Method with Arithmetical Averages (UPGMA). The amplification of genomic DNA from 45 clones yielded 2408 DNA fragments, ranging in size from 250 bp to 3000 bp. The range of genetic similarity matrix was very wide (59.18%-94.23%). It indicated that most of the clones have a low level of polymorphism. The lowest genetic similarity (59,18%) was found between RRIC 110 and AVROS 352 clones, while the highest (94.23%) was between IRR 41 and IRR 42 clones. Cluster analysis showed that 45 clones of rubber were divided into two groups, the biggest group consisted of 30 clones, while the other one consisted of 15 clones with a genetic similarity value of 0,73.
Litter decomposing fungi in sal (Shorea robusta) forests of central India KRISHNA KANT SONI; ABHISHEK PYASI; RAM KEERTI VERMA
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030305

Abstract

Abstract. Soni KK, Pyasi A, Verma RK. 2011. Litter decomposing fungi in sal (Shorea robusta) forests of central India. Nusantara Bioscience 3: 136-144. The present study aim on isolation and identification of fungi associated with decomposition of litter of sal forest in central India. Season-wise successional changes in litter mycoflora were determined for four main seasons of the year namely, March-May, June-August, September-November and December-February. Fungi like Aspergillus flavus, A. niger and Rhizopus stolonifer were associated with litter decomposition throughout the year, while Aspergillus fumigatus, Cladosporium cladosporioides, C. oxysporum, Curvularia indica, and C. lunata were recorded in three seasons. Some fungi including ectomycorrhiza forming occur only in the rainy season (June-August) these are Astraeus hygrometricus, Boletus fallax, Calvatia elata, Colletotrichum dematium, Corticium rolfsii, Mycena roseus, Periconia minutissima, Russula emetica, Scleroderma bovista, S. geaster, S. verrucosum, Scopulariopsis alba and four sterile fungi. Fungi like Alternaria citri, Gleocladium virens, Helicosporium phragmitis, and Pithomyces chartarum were rarely recorded only in one season.
Variation in oil contents, and seed and seedling characteristics of Jatropha curcas of West Nusa Tenggara selected genotypes and their first improved population BAMBANG BUDI SANTOSO
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030304

Abstract

Abstract. Santoso BS. 2011. Variation in oil contents and seed and seedling characteristics of Jatropha curcas of West Nusa Tenggara selected genotypes and their first improved population. Nusantara Bioscience 3: 130-135. This study describes variation in seed and seedling characters of Jatropha curcas Linn. of West Nusa Tenggara selected genotypes. Exploration was conducted in several areas where large population of this species grown as fences were found. Five selected genotypes were then grown in experimental fields to let mass selection to obtain the first improved population for each genotype. Seeds of wild population (P0) and those of selected trees as the first improved population (IP-1) were subjected to this study. Seed and seedling characteristics were measured. The result indicated that considerable genetic variability existed among the five J. curcas of West Nusa Tenggara selected genotypes and within each genotype population for seed and seedling characteristics. Genotypes of West Lombok, Sumbawa, and Bima performed exceedingly better than those of Central Lombok and East Lombok. Therefore, this study has suggestions for identifying potential seed sources of J. curcas and these existing genetic variability provides breeders with materials in crop improvement program.
Biological screening of selected traditional medicinal plants species utilized by local people of Manokwari, West Papua Province OBED LENSE
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030306

Abstract

Abstract. Lense O. 2011. Biological screening of selected traditional medicinal plants species utilized by local people of Manokwari, West Papua Province. Nusantara Bioscience 3: 145-150. The aim of the research was to determine the presence of alkaloids and anti-microbial activity in extracts from selected medicinal plants from Manokwari District, West Papua, Indonesia. The method of alkaloid testing followed the standard phytochemical methods. The procedure of the Calibrated Dichotomous Sensitivity (CDS) test was used for the antimicrobial bioassays. Results of biological screening suggested that all but one of the 56 species tested contained different levels of alkaloids. Eleven species showed anti-microbial activity using bioassays of responses to two bacteria, Salmonella typhi and Klebsiella pneumoniae, and two fungi Candida albicans, and Cryptococcus neoformans; none of the plant extracts showed an antimicrobial effect against the bacteria Escherichia coli. Extract of Planconella sp. was the most active one as it showed activity against three different organisms (C. albicans, C. neoformans, and S. typhi).
Blood bacterial wilt disease of banana: the distribution of pathogen in infected plant, symptoms, and potentiality of diseased tissues as source of infective inoculums HADIWIYONO HADIWIYONO
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030307

Abstract

Abstract. Hadiwiyono. 2011. Blood bacterial wilt disease of banana: the distribution of pathogen in infected plant, symptoms, and potentiality of diseased tissues as source of infective inoculums. Nusantara Bioscience 3: 112-117. Bacterial wilt caused by blood disease bacterium (BDB) is the most important disease of banana in Indonesia. The disease was difficult to control due to by poorly understood of ecology and epidemiology of the disease. This paper reports the distribution of pathogen-infected plant, symptoms, and potentiality of diseased tissues as source of inoculums. For studying the distribution of BDB in diseased banana, a number of 14 points of plant organ tissue was sampled and the pathogen was detected by PCR using a couple of specific primer for BDB, 121F, and 121R. In addition to the detection of BDB using PCR, both external and internal symptoms were observed. All the points of detection were also used as source of inoculums to search the potentiality of the tissues as source of infective inoculums. The results showed that BDB was distributed systemically in infected banana. The pathogen infection caused systemic symptom and all part of infected banana were potential as source of infective inoculums.

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