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Iman Rusmana
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rusmana13@yahoo.com
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microbiology.indonesia@gmail.com
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 6 Documents
 1 
Search results for , issue "Vol. 13 No. 1 (2019): March 2019" : 6 Documents clear
Growth Characteristics of Chikungunya Virus Isolate from Indonesia in Various Human Cell Lines in vitro Oktaviani Naulita Turnip; OKTAVIANI N. TURNIP; RAHMA F. HAYATI; RIZKA ALAWIYAH; BENEDIKTUS YOHAN; DIONISIUS DENIS; ANOM BOWOLAKSONO; AMIN SOEBANDRIO; R. TEDJO SASMONO
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1632.863 KB) | DOI: 10.5454/mi.13.1.1

Abstract

Chikungunya (CHIK) fever, a febrile illness caused by Chikungunya virus (CHIKV) infection, is one of mosquito-borne viral diseases affecting people living in the tropical and subtropical regions in the world. The pathogenesis of the disease is yet to be completely unraveled, and research on CHIK has been conducted by employing various methods, including using cell lines to investigate the biological characteristics of CHIKV in vitro. To assess the suitability of human cell line model for CHIK study, various human cell lines including A549, Huh7, and HepG2 were infected with CHIKV and assayed for their susceptibility to infection. The MTT and plaque assay methods were performed to measure cell viability and virus growth kinetics, respectively. Fluorescence-activated Cell Sorting (FACS) and immunofluorescence assay were performed to measure the proportion of infected cells in the system and their morphological visualization. Both A549 and Huh7 human cell lines showed stable high cell viability upon infection while CHIKV growth kinetics were significantly lower in these cells compared to Vero-CCL81, a monkey cell line that is routinely used in other arboviruses research. Interestingly, we observed significantly different results in HepG2 human cell line, in which cell viability and CHIKV growth kinetics were significantly higher. FACS and immunofluorescence assay confirm the higher infection rate of CHIKV in HepG2 than A549 human cell line. We concluded herethat human hepatocytes HepG2 cell line was susceptible to Asian Genotype of CHIKV and proposed as an alternative cell for the in vitro CHIKV studies to the commonly used A549 and Vero cells.
Isolation of a Functional Gene Encoding Homologous Lysophospholipase from Indonesian Indigenous Bacillus halodurans CM1 SHANNI FERNANDA; ABINAWANTO ABINAWANTO; IS HELIANTI
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (964.297 KB) | DOI: 10.5454/mi.13.1.2

Abstract

Lipase is a biocatalyst widely used in industry, for example detergent, pharmaceutical, food, or oil purification. One of the most widely lipase used for oil purification is lysophospholipase. As much as 50% of industrial enzyme needs are supplied from microorganisms. However, enzyme productivity from wild type microbial strain is usually limited and not applicable in industry, so that genetic engineering is necessary. Cloning gene encoding for lysophospholipase from Aspergillus niger and Cryptococcus neoformans have been conducted, but has never been conducted from alkalothermophilic bacteria, such as Bacillus halodurans. Bacillus halodurans CM1 is an alkalothermophilic bacterial strain isolated previously that has many industrially potential enzymes. This study aimed to isolate one of the gene encoding lipase from Bacillus halodurans CM1 and cloned into Escherichia coli DH5α using the pGEM-T easy vector. The gene fragment encoding lysophospholipase obtained with size 783 base pairs and had 100% similarity with gene encoding lysophospholipase from Bacillus halodurans C-125 (No access GenBank: BA000004.3). E. coli harbouring the recombinant plasmid with the gene also showed activity on trybutiryn medium compared to negative control.
ENDOPHYTIC FUNGI IN Paraserianthes falcataria: PRODUCTION OF INDOLE ACETIC ACID REINE SUCI WULANDARI; ROSA SURYANTINI
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2614.494 KB) | DOI: 10.5454/mi.13.1.3

Abstract

Identification of endophytic fungi in Paraserianthes falcatria is the effort of the potential of endophytic fungi as phytohormone producer.  Phytohormone is needed to spur shoot and root initiation.  This study in P. falcatariais necessary when woody of P. falcataria decreases every year.  The aimed of the study were to identify endophytic fungi from leaves, twigs, and roots of P. falcataria, and determine IAA content from endophytic fungi.  Isolates that were grown from leaves, twigs and roots cuttings on PDA, were identified based on micro- and macromorphology. Determining of IAA content was counted with spectrophotometer vis based on a calibration curve from the standard solution.  The results were obtained 10 of isolates fungi from leaves, twigs, and roots.  But from 10, only nine isolates that could be identified.  They were Aspergillus sp., Acremonium sp., Cladosporium sp., Trichoderma sp. 1, Phytium sp., Rhizoctonia sp., Trichoderma sp. 2, Hormiscium sp. 1 and Hormiscium sp. 2.  Production of indole acetic acid (IAA) from Cladosporium sp. had the highest content than others (311 ppm).  The lowest IAA content (51.97 ppm) was produced by the Rhizoctonia sp.  The study can be continued to find out their abilities as PGPF agents and biopesticides of P. falcataria seedlings.
Induced defense related enzyme activities of tomato plant by indigenous endophytic bacteria and challenged by Ralstonia syzigii subsp. indonesiensis YULMIRA YANTI; WARNITA WARNITA; REFLIN REFLIN
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2560.561 KB) | DOI: 10.5454/mi.13.1.4

Abstract

Our previous research had screened 9 best indigenous endophytic isolates for their ability to control Ralstonia syzigii subsp. indonesiensis, the causal agents of bacterial wilt disease in tomato (Lycopersicon esculentum) in green house condition. Those 9 strains were Bacillus cereus EPL1.1.3, B. cereus TLE2.3, B. toyonensis EPL1.1.4, Serratia nematodiphila TLE1.1, B. anthracis SNE2.2, B. cereus E1.AB1.2, B. cereus E1AB2.1, Enterobacter cloacae subsp. dissolvens TLE2.2 and S. marcescens KLE3.3. The purposed of this study is to test the ability of the endophytic bacteria strains in increasing defense related enzyme activities of tomato. Bacterial strains were tested for its ability to induce the defense-related enzymes which were phenylalanine ammonia lyase (PAL), peroxidase (PO) and polyphenol oxidase (PPO) in roots and leaves of tomato plants. R. syzigii subsp. indonesiensis inoculated to host plants 7 days after the endophyte bacteria strains inoculation. Enzyme activities were recorded at 0, 1, 3, 5, 7, 9, 12 and 15 days after pathogen inoculation (dpi).  It was observed that PAL, PO and PPO activities were significantly increased in all of the endophytic bacteria inoculated treatments compared to control plant. Activities of PAL in the leaves was fast similar to the roots; but PO activities was higher in the roots compared to that in the leaves, whereas PPO activities was higher in the leaves than in the roots. PAL and PO reached the maximum level at different time in the leaves (3 dpi and 15 dpi), in the roots (5 dpi and 12 dpi), whereas PPO in the leaves at 12 dpi and in the roots at 9 dpi.
Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line FITRIYAH SJATHA; OCTAVIA CHANDRA MUSTIKA; ANGKY BUDIANTI; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1505.897 KB) | DOI: 10.5454/mi.13.1.5

Abstract

Dengue infection is a global infectious disease with almost 100 million cases occur annually in over more than 100 endemic countries. Dengue virus (DENV), the causative agent of dengue infection, is an 11 kbp RNApositive strand virus which encode 3 structural and 7 non-structural protein within its genome. Non-structural 1 (NS1) protein of DENV is expressed in the earlier stage of infection and having pathogenic role in disease severity. NS1 gene of DENV serotype-2 Indonesian strain was amplified through PCR method using specific designated primers. NS1 amplicon were then cloned into pUMVC4.a and pcDNA3.1 mammalian expression vector which confirmed through colony PCR and sequencing method. Recombinant pUNS1 and pcNS1 plasmids were transfected into CHO-K1 mammalian cell line with lipid based method. Recombinant NS1 protein expression were analyzed through immunostaining using dengue patient sera and rapid NS1 detection kit. Recombinant pUNS1 and pcNS1 plasmids were successfully constructed and recombinant NS1 protein was expressed in CHOK1 mammalian cell line and shown to be reactive against dengue patient sera. Our recombinant NS1 protein also tend to be released outside the transfected CHO-K1 cells as detected in rapid NS1 detection kit. Recombinant dengue NS1 protein was expressed in mammalian cell line in both intra and extracellularly and shown to be immunogenic.
ITA REGISTRATION FORM AND BACK COVER Iman Rusmana
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1664.116 KB) | DOI: 10.5454/mi.13.1.%p

Abstract

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