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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 11 No. 1 (2021)" : 5 Documents clear
Risk Prevention of Ventilator-Associated Pneumonia Thorough Oral Hygiene: A Literature Review Musdalipah Musdalipah; Yuliana Syam; Takdir Tahir
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.05

Abstract

Ventilator-Associated Pneumonia (VAP) is a type of nosocomial infection that mostly occurs in intensive care units, generally occurring 48 hours after intubation. Endotracheal intubation and the use of a mechanical ventilator are invasive measures by patients, having a therapeutic effect. This paper aimed to identify the use of oral hygiene materials for the incidence of pneumonia related to the use of a ventilator. The research design was by searching literature in five databases, namely PubMed, Proquest, Science Direct, Wiley Online Library, and Google Scholar, by taking all databases in English and Indonesian, published in the last ten years (2010-2020). There were six articles about the effectiveness of using oral hygiene as risk prevention for Ventilator-Associated Pneumonia (VAP) with various concentrations and volumes. Several oral health assessment tools that can be used are Oral Assessment Guide, Beck Oral Assessment Scale, or Mucosal-Plaque Score. Comprehensive implementation of oral hygiene can improve the oral health of patients with mechanical ventilation, so bacterial colonization that causes VAP can be prevented. Therefore, nurses need to understand and apply oral assessment instruments as a basis for giving intervention.Keywords: Intensive care unit, Oral hygiene, Ventilator-Associated Pneumonia
Genetic Variation Analysis of Four Local Varieties of Indonesian Black Rice (Oryza sativa L.) Based on Partially rbcL cpDNA Gene Sequence Abdul Basith; Estri Laras Arumingtyas; Widodo Widodo
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.01

Abstract

Black rice (Oryza sativa L.) varieties i.e. Toraja (South Sulawesi), Cempo Ireng (Yogyakarta), Wojalaka (East Nusa Tenggara), and Manggarai (East Nusa Tenggara) are four local black rice varieties in Indonesia whose character has not been widely studied, especially the character of genetic variation. Research aimed to determine the variation of the rbcL gene in the four local black rice varieties. The sample for testing the variation of the rbcL gene sequence in the form of black rice leaves six weeks after planting. Dendogram was carried out using the UPGMA method with the Kimura 2-parameter algorithmic calculation model using the MEGA5 version 5.2.2 program. The results showed that partially the rbcL gene sequence was successfully amplified on four black rice varieties with a sequence length of 487 bp. The partial rbcL sequence of black rice consisted of 26.58% tyrosine, 21.38% cytosine, 28.86% adenine, and 23.18% guanine. The value of G + C content was 0.446, with the frequency of invariable sites of 97.13%. The frequency of informative parsimony sites was 1.43% with a nucleotide diversity (Pi) value of 42-10, the number of haplotypes was 5, and the total number of mutations and polymorphic sites was 14. The ratio between transition and transversion (ts/tv ratio k) for purine bases was 1.741 and pyrimidine was 3.571, with the estimated overall ratio between transition and transversion (R) of 1.31. Based on the dendogram, the farthest genetic distance was found in Wojalaka and Manggarai varieties, which were 0.019 respectively.Keywords: black rice, genetic variation, local varieties, rbcL gen
Minnow Trap Color Effectiveness Test Using Cat Food Bait as Aquatic Sampling Gear on Diurnal Fish in Gajah Mungkur Reservoir, Cental Java, Indonesia Adityas Arifianto; Elsa Mufti; Agung Pramana Warih Marhendra; Nia Kurniawan
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.04

Abstract

The Minnow Trap is a simple sampling gear, efficient, easy to operate, affordable, easy to make, and has been used in research in the fisheries and aquatic ecology for more than 90 years. The brightness, color, and visual obstruction of fishing gear or fish traps affect the behavioral response of target fish, considering that each fish has a specific level of color sensitivity. The color effectiveness test of fishing gear is an important prerequisite for the use of sampling tools, and it needs to be evaluated to understand the gear performance and avoid potential sampling bias. The purpose of this study was to test the color effect of the minnow trap on diurnal fish in the Gajah Mungkur Reservoir waters. The research was carried out in Gajah Mungkur Reservoir, Wonogiri Regency, at two stations. The sampling conducted at 09.00 - 11.00 and 13.00 - 15.00 Indonesian West Time, where the initial survey is estimated to be the highest time for diurnal fish activity. The minnow trap used in this study is four colors vinyl-painted double funnel cylindrical minnow trap with a 5x5 millimeter mesh size and a conventional umbrella minnow trap used as control. The sampling results are then recorded on the datasheet and analyzed using Microsoft Excel. CPUE (Catch Per Effort Unit) of each unit is then calculated and statistically analyzed by Kruskal-Wallis test and Kolmogorov-Smirnov normality test through SPSS 25.0 software. The test result of five unit minnow traps from 40 installations and two hours of deployment time, 80 individuals were captured, consisted of seven species of freshwater fish. CPUE values ranking consecutively from the highest from silver units (1.375), black (1.25), green (1.125), red (0.9375), and control (0.3125). The Kruskal-Wallis analysis showed that all tested units do not have a significant difference. Keywords: Color, catch per unit effort, fish, minnow trap.
The Sensitivity of Leersia hexandra Sw. to Gamma-Ray Irradiation Pritha Kartika Sukmasari; Wahyu Widoretno; Dian Siswanto
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.02

Abstract

Gamma-ray irradiation as a physical mutagen has high penetrating power. Therefore, it is most often used to increase genetic variability or produce new mutant plants. This research was conducted to obtain the lethal dose of gamma-rays in Leersia hexandra plants. The used plant part was a single node stolon which had a length of 10 cm with the node in the middle of the stolon. The irradiation doses given were 0, 25, 50, 75, 100 Gy. Stolons were inserted into plastic clips and irradiated using a Cobalt-60 gamma irradiation source at the Center for Irradiation and Radioisotope Applications (PAIR), National Nuclear Energy Agency (BATAN) Jakarta. Gamma-irradiation has a significant effect on inhibiting shoot growth. The growth of irradiated Leersia hexandra with the best number of plants and the highest shoots was obtained at a dose of 25 Gy and decreased with increasing irradiation dose. The lethal dose (LD50) was determined eight days after irradiation using CurveExpert 1.4 software. Leersia hexandra plants that can sprout and regenerate followed the linear equation y = 1.02 - 7.5x with LD50 at 68.85 Gy and LD20 at 29.36 Gy.Keywords: gamma irradiation, genetic variation, Leersia hexandra, lethal dose, mutant plant.
Study of Integrated Pest Management Strategy on The Population of Fruit Flies (Bactrocera spp.) in Red Chili Cultivation (Capsicum Annuum) Wildan Muhlison; Nanang Tri Haryadi; Agung Sih Kurnianto; Bugar Syarif Ahmada
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.03

Abstract

The fruit fly Bactrocera spp. is the main pest other than Thrips in red chilies, which can reduce plant productivity by 30-60%, so that a specific method of handling this pest is needed. This study examines the application of conventional and Integrated Pest Management (IPM) strategies to fruit fly populations in red chili cultivation (Capsicum annuum). Observation of fruit fly population used the comparative method with methyl eugenol traps and incubation of infected fruit. Determination of the research sample based on purposive sampling method and analyzed using descriptive analysis. The test parameters were the fruit fly population indicated by the host's density and hosts' availability in the applied IPM and conventional treatments. The results showed that the fruit fly species encountered were dominated by B. dorsalis with a percentage of 98.18% and B. carambola 1.82%. The fruit fly population's fluctuation in IPM and conventional treatments were significantly different, as evidenced by a one-way variance test at a significance level of 99%. The population of fruit flies in the IPM concept was 547 flies, while the conventional concept was 1546 flies. The percentage of fruit fly population in red chili plants with IPM treatment was 48% smaller than conventional treatments.Keywords: Bactrocera spp., IPM, Population, Red chilies.

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