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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 11 No. 2 (2021)" : 6 Documents clear
Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer Uswatun Hasanah; Tri Ardyati; Dian Siswanto
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.02

Abstract

Previous research stated that bacteria isolated from sago waste from the traditional sago industry in Palopo had the potency to produce amylase. This study aims to confirm the ability of bacteria isolated from sago pulp waste to produce amylase, evaluate the ability of these amylolytic bacteria to produce IAA fix nitrogen, identify selected bacteria, and apply selected bacteria into granules biofertilizers. Bacteria were isolated from sago pulp waste and grown on a 1% starch agar medium. The amylolytic activity was analyzed qualitatively using iodine and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH 6. Amylolytic bacteria were analyzed for IAA production using Salkowsky reagent and nitrogen fixation ability through the Serra Kit method. Potential bacteria were identified based on the similarity of the 16S rDNA sequence. The selected bacteria were grown in a 10mL NB medium, and then bacteria were inoculated and air-dried to obtain the granules. The selected bacteria were put into granules and re-isolated using the total plate count (TPC) method. Eight amylolytic bacteria were obtained from sago pulp waste. L1E isolate had the highest amylolytic activity of 1.228 U.mL-1, and L1D isolate had the highest IAA production of 69.8 g.mL-1. Isolate L1E was identified as Alcaligenes faecalis with a 99.45% similarity index, and L1D was identified as Serratia surfactantfaciens with a 99.09% similarity index. Isolate L2G was identified as Alcaligenes aquatilis with a similarity index of 99.8% of the 16S rDNA sequence. The viability of bacteria from granules was 1.41 x 108 CFU.g-1.Keywords: Amylase enzyme, amylolytic bacteria, granulated fertilizer.
The Effectiveness of Weed as Beetle Bank Against Abundance of Soil Arthropods on Corn (Zea mays. L) Anis Sa'adah; Nanang Tri Haryadi
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.05

Abstract

Conservation of natural enemies on maize can be done by utilizing grass weeds as Beetle banks. Natural enemies of shelter and get food from the weed grasses that are in the land irrigated corn. Natural enemies observed in this research were the predator of ground arthropods in the form of ground beetles, tomcat, and predators arthropods in other lands. The trap used is the Pitfall trap. The percentage of arthropod predators was 69%, and arthropods herbivore was 31%. The abundance of soil arthropods in the treatment of weeds grass is higher than the control treatment It is because of the treatment of weed grass soil arthropods get habitat for a place to stay. Weed grasses provide food sources for the arthropods and get protection from an environment that is less supportive. The use of weed grasses in addition to Beetle banks also serves as a place to breed. The highest abundance of arthropods was situated on the treatment Eleusine indica that has a lush lawn and has a characteristic odor favored by arthropods. Arthropods that acts as a predator in the land are the family of Formicidae, Philodromidae, Carabidae, Staphylinidae, Pentatomidae. Some Arthropods from the Pentomidae family have a role as a pest and others as natural predators or predators. Arthropods found in the research was an arthropod that had a role as natural enemies or predator. The Pentomidae family that has a position as a predator is Picromerus Bidens. Ground beetles were found in the C. fossor and Pheropsophus sp. Ground beetles are dominant picking weeds Setaria sp. and Eleusine indica as habitat and place in search of food and life's survival. Keywords: Beetle bank, soil arthropods, weeds.
Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Angie Via Resty Kimbal; Yoga Dwi Jatmiko; Tri Ardyati
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06

Abstract

The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.
Analiysis of Phytoplankton Diversity on the Productivity of Vannamei Shrimp (Litopenaeus vannamei) Intensive Pond, Jatisari Village, Banyuwangi Nabilla Anggi Juliyanto; Maftuch Maftuch; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.01

Abstract

Phytoplankton can be used to estimate the potential for vannamei shrimp production. It can be used as a provider of nutrient sources and has an important role in improving water quality. This study aims to analyze the phytoplankton community structure on the productivity of intensive vannamei shrimp ponds. The research was conducted in Jatisari Village, Banyuwangi, East Java, Indonesia. Carried out parameters were the calculation of density, phytoplankton diversity index, culture performance (SR, FCR, ADG), and water quality (temperature, water transparency, salinity, pH, DO, NO2, NO3, PO4, NH4). The results showed that there were six classes and 33 genera from both ponds, Chlorophyceae (10 genera), Bacillariophyceae (8 genera), Cyanophyceae (9 genera), Dinophyceae (3 genera), Euglonophyceae (1 genus), Criptophyceae (1 genus). The index value of the two ponds shows moderate diversity, H 'pond 1 is 1.76, and pond 2 is 2.02. The two plots' cultivation performance was SR 92% and 80%, FCR 1.08 and 1.13, ADG 0.31 g.day-1 and 0.35 g.day-1, respectively. The physical and chemical parameters of the research showed a good enough value for the life of vannamei shrimp and phytoplankton.Keywords: Cultivation performance, Phytoplankton, Vannamei shrimp.
Phytoplankton and Its Relationship to White Leg Shrimp (Litopenaeus vannamei) Culture Productivity in Alasbulu, Banyuwangi Hasna Kamilia; Bambang Budi Sasmito; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.03

Abstract

Shrimp culture is related to phytoplankton's existence as a primary producer and water quality. Aside from their function as natural feed, phytoplankton has a role in maintaining the stability of the pond ecosystem. It is indicated by the high abundance and diversity of phytoplankton, as well as suitable water quality to support the productivity of shrimp culture. This research aimed to determine the correlation between phytoplankton structure community, water quality parameters, and shrimp productivity. This research used a descriptive method. This research was conducted in shrimp culture intensive system Alasbulu Village, Wongsorejo District, Banyuwangi on February-March 2020. The parameters observed are diversity and abundance of phytoplankton, water quality parameters, and production performance in each pond. Based on the results, six phytoplankton classes were identified: Bacillariophyceae (10 genera), Cyanophyceae (8 genera), Chlorophyceae (5 genera), Dinophyceae (2 genera), Euglenophyceae (1 genus), and Cryptophyceae (1 genus). Chlorophyceae dominated both ponds, followed by Cyanophyceae. Diversity index values on ponds 1 and ponds 2 were 1.39 and 1.50, respectively. Productivity of both ponds were 1.8 kg.m-2 and 1.4 kg.m-2; FCR (Feed Convertion Ratio) 1.1 and 1.3; ADG (Average Daily Growth) 0.3 g.day-1 and 0.25 g.day-1. It can be concluded from this research that high density of Chlorophyceae in phytoplankton community is one of the main causes that supported shrimp cultivation. Keywords: Banyuwangi, Litopenaeus vannamei, phytoplankton, productivity. 
Effect of Prebiotic and Probiotic Fish Feed on Physical, Chemical and Biological Quality of Feed M. Indra Wahyu Pratama; Anik Martinah; Ating Yuniarti
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.04

Abstract

The limitations of fish in digesting food depend on the presence of enzymes protease, amylase, and lipase that react with substrates in the digestive channel of fish. Supplementation methods can be performed to increase fish growth by adding prebiotics and probiotics to the feed. This study aims to determine the influence of the difference in the length of time fermented prebiotic feed (sweet potato extract) and probiotics (Bacillus megaterium) on the quality of feed. The results of the physical analysis of fish feed showed the color looks brown, fishy smell, texture, and general conditions of feed seemed completely normal. Chemical analysis showed that the proteins were best improved in B3 treatment, with the period of fermentation approximately 72 hours. Biological analysis suggests that the longer the fermentation time taken, the higher the abundance of bacteria obtained.Keywords: B. megaterium, chemistry and biology of fish feed, physical analysis, sweet potatoes (Ipomoea batatas L)

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