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Uji Aktivitas Bakteriofage Litik dari Limbah Rumah Tangga Terhadap Salmonella Typhi Yoga Dwi Jatmiko; Agung Putra Purwanto; Tri Ardyati
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3471

Salmonella Typhi merupakan salah satu bakteri yang menjadi agen penyakit bawaan makanan. Bakteriofage sebagai alternatif penggunaan antibiotika telah digunakan untuk mengendalikan bakteri tersebut. Penelitian ini dilakukan untuk mendapatkan isolat bakteriofage litik yang mampu melisis beberapa bakteri patogen yang diujikan dan mengetahui pengaruh aktivitas bakteriofage litik terhadap pertumbuhan SalmonellaTyphi. Bakteriofage diisolasi dari limbah rumah tangga. Selanjutnya penentuan host range bakteriofage terhadap bakteri patogen lain dilakukan dengan metode spot test. Uji aktivitas bakteriofage terhadap SalmonellaTyphi dilakukan menggunakan metode bacterial challenge test. Berdasarkan hasil isolasi, didapat enam isolat bakteriofage, yaitu B2-St, B3-St, S1-St, S2-St, SL1-St, dan SL3-St. Semua isolat bakteriofage mampu melisiskan sel bakteri Escherichia coli dan Salmonella Typhimurium namun tidak mampu melisiskan Bacillus cereus, Staphylococcus aureus dan Shigella disentriae.Tiga isolat bakteriofagetelah terpilih berdasarkan densitas plaque terbanyak yaitu B2-St, SL3-St dan S2-St. Kemampuan isolat bakteriofage B2-St dalam melisiskan sel Salmonella Typhi lebih tinggi (6,81 ± 0,35 log sel/mL) daripada isolat bakteriofage SL3-St (7,39 ± 0,31 log sel/mL) dan S2-St (7,60 ± 0,27 log sel/mL). Penurunan densitas sel inang terendah oleh ketiga isolat bakteriofage terjadi pada jam ke-4. Bakteriofage B2-St merupakan bakteriofage terbaik dan berpotensi sebagai agen biokontrol Salmonella Typhi.

Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa Alifia Issabella Mulyawati; Tri Ardyati; Yoga Dwi Jatmiko
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4194

Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations.

Profile of microbial community of naturally fermented Sumbawa mare’s milk using next-generation sequencing Yoga Dwi Jatmiko; Irfan Mustafa; Tri Ardyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

This study aimed to investigate the bacterial and fungal/yeast diversity in naturally fermented Sumbawa mare’s milk through a next-generation sequencing approach, and evaluate the quality of fermented mare’s milk based on the presence of pathogenic or undesirable microorganisms. Microbial density determined using plate count agar (total aerobic bacteria), de Man Rogosa Sharpe agar (Lactobacillus), M17 agar (Lactococcus) and yeast peptone dextrose agar supplemented with streptomycin 50 ppm (yeast). Nutritional content and acidity level of each fermented milk sample were also evaluated. Genomic DNA was extracted using FastDNA Spin (MPBIO). The total gDNA was further analyzed using illumina high-throughput sequencing (paired-end reads), and the sequence results were analysed using QIIME v.1.9.1 to generate diversity profiles. The difference in nutrient content of mare’s milk was thought to affect the density and diversity of microbes that were able to grow. Fermented mare’s milk samples from Sumbawa had the highest bacterial diversity compared to samples from Bima and Dompu. However, fermented mare’s milk from Dompu had the best quality which was indicated by the absence of bacteria that have the potential to be pathogenic or food spoilage, such as members of the Enterobacteriaceae family (Enterobacter, Klebsiella and Escherichia-Shigella) and Pseudomonas. Genus of Kazachstania and Kluyveromyces, as well as family Dipodascaceae were frequently observed fungi/yeast from Sumbawa fermented mare’s milk. The presence of potential pathogenic bacteria warrants special attention in improving the hygiene of manufacturing process.

HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.

PEMBERDAYAAN PEREMPUAN SEBAGAI AGEN PENGGERAK DALAM PENGOLAHAN SAMPAH DAPUR MENJADI ECO ENZYME Agustina Shinta Hartati Wahyuningtyas; Anisa Aprilia; Tri Ardyati; Kiki Fibrianto; Fahriyah Fahriyah; Riyanti Isaskar; Arie Srihardyastutie; Destyana Ellingga Pratiwi; Vi’in Ayu Pertiwi; Yusri Fajar
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 1 (2023): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i1.12000

Abstrak: Peningkatan jumlah penduduk mengakibatkan adanya peningkatan produksi sampah, utamanya limbah organik rumah tangga yang dapat menimbulkan dampak buruk bagi lingkungan maupun kesehatan masyarakat jika tidak diimbangi dengan pengelolaan sampah yang tepat. Tujuan kegiatan pengabdian ini adalah untuk mengembangkan pengetahuan, kesadaran, dan kebiasaan baik terutama bagi kaum perempuan agar mampu mengolah sampah atau limbah rumah tangga menjadi eco enzyme. Metode pengabdian meliputi persiapan, sosialisasi, dan pelatihan. Mitra dari kegiatan ini adalah kelompok ibu-ibu PKK RW VII Kelurahan Lesanpuro, Kecamatan Kedungkandang, Kota Malang dengan jumlah peserta yang terlibat sebanyak 86 20 orang. Hasil dari kegiatan tersebut, yaitu peserta dapat membuat dan melestarikan eco enzyme yang didukung dengan hasil evaluasi di mana peserta memiliki peningkatan pemahaman terkait pengolahan sampah dari aspek pengetahuan, kebiasaan, dan kepedulian.Abstract: The growth of population causes the increase of waste production, especially household waste which can cause a negative impact for environment and health if it isn't used the right waste’s management. The community service aims to increase the knowledge, awareness, and good habits to manage household waste into eco enzymes. The first method of this service is preparation, and then followed by socialization. And also training. The partners are the women of RW VII, who are the member of PKK group in the Kelurahan Lesanpuro of Kecamatan Kedungkandang, Kota Malang. There are 86 women involved. The result of the activity shows that participants can make and preserve eco enzymes, which is supported by the evaluation result. It shows that the participants understanding about organic waste management have increased based on the aspects of knowledges, habits, and care.

HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.

PENDAMPINGAN PENGELOLAAN SAMPAH ORGANIK DAPUR BERSAMA IBU RUMAH TANGGA DAN UNIT USAHA TPST BUMDES MENGGUNAKAN PARTISIPATORY RURAL APPRAISAL Fahriyah Fahriyah; Tri Ardyati; Yusri Fajar; Vi’in Ayu Pertiwi; Anisa Aprilia; Riyanti Isaskar; Zamratul Akbar; Ilyaza Agung Himawan; Dwi Gatot Sabarno; Wheny Anggi Anggraini; Khoirunnisa Bai
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 6 (2023): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i6.19378

Abstrak: Pengelolaan sampah di Indonesia merupakan suatu hal yang sangat memprihatinkan, dimana sampah yang terorganisir memberikan tantangan yang besar dan menimbulkan berbagai akibat yang merugikan, termasuk pencemaran lingkungan yang dapat berdampak buruk pada ekosistem. Sehingga penelitian ini bertujuan untuk meningkatkan kesadaran dan keterampilan masyarakat serta mengubah perilaku dalam penanganan limbah sampah organik. Salah satu daerah yang terdapat timbulan sampah adalah TPST BUMDes Sumber Sejahtera Desa Pujon Kidul. Masyrakat dan BUMDes dijadikan sebagai mitra dengan kontribusi sebagai penyedia sarana dan penggerak untuk terlibat dalam program pendampingan, jumlah masyarakat yang terlibat langsung dalam kegiatan ini sebanyak 149 responden. Metode yang digunakan ialah pendekatan Partisipatory Rural Appraisal (PRA), metode yang mengikut sertakan masyarakat dalam proses membangun dan mengembangkan suatu kegiatan. Hasil yang diperoleh adalah peningkatkan pengetahuan mengenai pengolahan sampah organik dapur kepada Ibu Rumah Tangga dan unit usaha TPST BUMDes Sumber Sejahtera sebesar 62% yang ditinjau dari penilaian Pre-test dan Post-test.Abstract: Waste management in Indonesia is a matter of great concern, where organized waste poses a great challenge and causes various adverse consequences, including environmental pollution that can adversely affect the ecosystem. This research aims to increase community awareness and skills and change behavior in handling organic waste. One of the areas where waste is generated is TPST BUMDes Sumber Sejahtera Pujon Kidul Village. The community and BUMDes are used as partners with contributions as providers of facilities and drivers to be involved in the mentoring program, the number of people directly involved in this activity is 149 respondents. The method used is the Participatory Rural Appraisal (PRA) approach, a method that includes the community in the process of building and developing an activity. The results obtained were an increase in knowledge about processing organic kitchen waste to housewives and the TPST BUMDes Sumber Sejahtera business unit by 62% which was reviewed from the Pre-test and Post-test assessments.

HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.

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