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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 14 Documents
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Search results for , issue "Vol 27, No 01 (1995)" : 14 Documents clear
The Effects of new T cell line derived lymphokines on B cell activation Sofia Mubarika Haryana, Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Telah diketahui bahwa untuk aktivasi limfosit B diperlukan faktor pertumbuhan yang disebut limfokin atau sitokin. Banyak bukti menunjukkan bahwa limfosit T yang teraktivasi menghasilkan substansi yang dapat memacu proliferasi atau diferensiasi limfosit B. Dewasa ini telah dapat diidentifikasi berbagai limfokin yang disebut sebagai interleukin (IL) seperti IL-2, IL-3, IL-4, IL-5, IL-6 dan IL-7 yang ikut berperan dalam aktivasi sel B. Namun, mekanisme aktivasi sel B oleh limfokin sampai sekarang masih terus berkembang dan dipelajari. Untuk dapat lebih memahami aktivasi sel B, dalam penelitian ini telah dilakukan pembuatan suatu T cell line, dan telah dikarakterisasi, yakni menunjukkan fenotipe Thyl+, Lyt1+2+, Dalam penelitian ini berbagai aktivator poliklonal lipopolysaccharide (LPS), ConA, dextran sulfat dipakai untuk memacu T cell line menghasilkan limfokin. Aktivitas limfokin pada supernatannya kemudian dianalisis dengan menggunakan blastogenesis assay dengan 3H-thymidine dan hemolytic plaque assay.Hash penelitian menunjukkan bahwa T cell line menghasilkan faktor pertumbuhan yang memacu proliferasi sel B (B cell growth factor II = BCGF II atau IL-5) dan faktor yang memacu sel B untuk berdiferensiasi (B cell differentiating factor = BCDF atau IL- 6) dan menghasilkan igM dan IgG.Key words : blastogenesis assay -- hemolitic plaque assay -- B cell activation -- T cell line -- lymphokines
Post-operative complications after cataract surgery with pseudophakos implantation at Dr Sardjito hospital Suhardjo, Suhardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Universitas Gadjah Mada

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Abstract

Principally, complications of post operative cataract surgery with pseudophakos implantation cover those complications due to the operation, and those due to the existence of pseudophakos within the eye ball. In Dr. Sardjito hospital, this operation has been done since 1988. There are many kinds of complication of this operation. This retrospective study is limited to those complications in the form of anterior uveitis, endophthalmitis, secondary glaucoma, and endothelial decompensation which occurred within 1992-1994 period.A study has been performed on 405 cases, consisting of 397 cases with posterior chamber pseudophakos implantation and 8 cases with posterior chamber pseudophakos implantation. In the same period, a study on 432 cases with extra capsular cataract extraction without pseudophakos implantation was performed. The complication in the group with pseudophakos implantation were: anterior uveitis (2.96%), secondary glaucoma (1,48%), endhopthalmitis (0,99%), endothelial decompensation (0.74%) and other complications such as iris prolapse and subluxation of pseudophakos. The complication in the group without pseudophakos implantation were anterior uveitis (0.23%), endothelial decompensation (0.23%), and prolapse of iris 0.46%. In this study, the complication in the form of endophthalmitis occurred 1 week after surgery in 3 cases, and 2 weeks after surgery in one case. One case was caused by fungi infection. The complication in the form of secondary glaucoma mostly related with the occurence of post anterior uveitis surgery. The anterior uveitis in post cataract surgery with pseudophakos implantation was considered to be related with the kind of lens implanted.Key words : post operative complications -- cataract surgery -- pseudophakos implantation -- anterior uveitis -- kind bf lens implanted
Hyperbilirubinemia in the newborn infants; unbound bilirubin approach Achmad Surjono, Achmad Surjono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Universitas Gadjah Mada

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Abstract

A guideline for management of hyperbilirubinemia in the newborns based solely on total/indirect serum bilirubin levels is considered outmoded. An approach to the identification of newborns at risk of bilirubin toxicity due to the increase of unbound bilirubin is proposed. Unbound bilirubin can easily enter the brain and bind to the nerve cells.A peroxidase oxidation method using unbound bilirubin - analyzer (UB-analyzer) offers a rapid, accurate and reproducible measurement of unbound bilirubin. Phototherapy, either continuous or intermittent can be applied to term and preterm infants on the monitoring of unbound bilirubin levels.Temporary cessation of breast-feeding is not advised in healthy term newborns with hyperbilirubinemia. On the contrary, more frequent feeding is advocated. Caution and substitution should be taken in giving any drugs having a potent displacer on bilirubin-albumin binding, especially In premature infants.Key words : newborn infants --hyperbilirubinemia -- unbound bilirubin -- phototherapy -- displacing drugs
Hemophilia with intracranial bleeding - A case report Sunarto, Sunarto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Universitas Gadjah Mada

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Abstract

A hemophiliac boy was hospitalized due to head injury in the previous sixteen days. On admission the consciousness decreased and visus of both eyes was 1/on. CT scan showed blood clot in the area of dorsomedial of medial cerebral arteria and sign of hydrocephalus. A surgical procedure could not be taken due to lack of facilities (antihemophilic factor supply and assay of factor VIII activity). The patient was given 6 cryoprecipitate everyday and supportive treatment as indicated. Fortunately the patient recovered, no neurologic complication occurred except the visus of the right eye that was still 1/oo and the hydrocephalus decreased.Key words : hemophilia - laboratory diagnostics - head injury - hydrocephalus and decreased visus - cryoprecipitate
The Effect of budesonide on lymphoid and non-lymphoid cell profiles, and la-antigen expression in rats with experimental colitis Marsetyawan HNE Soesatyo, Marsetyawan HNE Soesatyo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Universitas Gadjah Mada

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Abstract

Kortikosteroid merupakan obat yang efektif terhadap peradangan, seperti penyakit peradangan usus kronis. Penelitian ini bertujuan untuk mengetahui pengaruh pamberian kortikosteroid lokal, yakni budesonide colitis experimental pada tikus, khususnya pengaruhnya terhadap populasi sel limfoid dan nonlimfoid, serta ekspresi antigen-la (molekul major histocompatibility complex (MHC) kias II). Di camping itu diteliti pula peran molekul-molekul adhesi, seperti intercellular adhesion molecules (iCAM-1) dan lymphocytes function associated antigen (LFA-1) setelah pemberian obat tersebut.Model colitis dibuat dengan pemberian secara intracolon zat hapten: 2,4,6-trinitrobenzene sulphonlc acid (TNBS) dalam etanol, berdasarkan metode standar. Obat budesonide diberikan deism deals tunggal dan ganda sebanyak 0,25 ml larutan 104 M secara lokal ke dalam colon dengan menggunakan kateter. Populasi sal limfoid dan nonlimfoid di sepanjang mukosa dan submukosa, termasuk plak Payer (PP) dan jaringan limfoid di daerah proksimal colon diperiksa dengan teknik imunositokimiawi. Ekspresi MHC Ida* ii dan molekul adhesi diteliti menggunakan panel antibodi monoklonal (AbMo).Dosis ganda budesonide (3x) *angst efektif untuk mengobati colitis akut; ini dapat diamati dad gejala-gejala klinis yang menghilang. Pemeriksaan histologik menunjukkan penurunan nyata jumlah subpopulasi makrofag, seperti sel EDI% ED2* dan ED3+. Intensitas ekspresi la dan ekspresi ICAM-1, LFA-1 mengurang, sdang lainnyaedangkan migrasi sal neutrofil dan sel radang lainnya menghilang.Key words : inflammatory bowel disease -- TNBS colitis -- budesonide-lymphoid and non-lymphoid cell populations -- MHC class II expression -- adhesion molecules.
Cell mediated immunity in malaria : 1. Changes in numbers of mononuclear phagocytes during plasmodium vinckei petteri infection in immunized and non-immunized mice Supargiyono, Supargiyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Penelitian bertahap telah dilakukanuntuk mempelajari reaksi-reaksi immunitas selular yang terjadi selama infeksi Plasmodium vinckei petteri malaria pada mencit LACA. Fokus kajian pada penelitian tahap pertama ini adalah mempelajari profil perubahan jumlah sel fagosit mononuklear (mononuclear phagocytes) dalam sumsum tulang, darah tepi dan dalam limpa, selama infeksi malaria pada mencit yang diimunisasi dan yang tidak diimunisasi. Imunisasi dilakukan dengan penyuntikan secara intravena 108 parasit stadium darah yang dibebaskan dari eritrosit dengan larutan saponin dan difiksasi dengan larutan formalin. Kedua kelompok mencit, yang diimunisasi dan yang tidak diimunisasi diinfeksi dengan 103 eritrosit terinfeksi, pada hari ke 15 sesudah imunisasi. Jumlah sel fagosit berinti satu beserta sel prekursornya dievaluasi berturut-turut pada hari ke 0, 3, 6, 9 dan 12 sesudah infeksi. Hasil penelitian menunjukkan bahwa selama infeksi P. v. petteri, jumlah sel fagosit mononuklear meningkat di dalam sumsum tulang, kemudian di dalam darah tepi dan dalam limpa. Derajat peningkatan jumlah sel fagosit tersebut lebih tinggi pada mencit yang telah diimunisasi sebelumnya dibandingkan dengan pada mencit yang tidak diimunisasi. Tingginya peningkatan jumlah sel fagosit mononuklear pada mencit yang diimunisasi tersebut nampaknya berkaitan dengan keberhasilan sistem kekebalan tubuh hospes dalam menurunkan parasitemia. Peningkatan jumlah sel fagosit tersebut didahului dengan peningkatan jumlah sel prekusornya di dalam sumsum tulang, sehingga bisa diantisipasikan bahwa produksi dan proliferasi dari sel prekursor di dalam sumsum tulang juga meningkat dalam usaha memenuhi kebutuhan sel d.ewasa di dalam darah dan jaringan selama infeksi malaria.Key words: Plasmodium vinckei petteri -- malaria -- LACA mice -- mononuclear phagocyte -- cellular immunity
Renoprotective effects of ACE inhibitors on the progression of diabetic kidney disease
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Functional and structural abnormalities in diabetic kidney disease lead to intraglomerular hypertension and albuminuria. Systemic hypertension and genetically inherited defect on autoregulation in preglomerular arterioles will enhance kidney injury in those patients. ACE inhibitors could reduce albuminuria In patients with diabetic nephropathy and animal models. Hemodynamic improvement manifested by the reduction of albuminuria by ACE inhibitors is only seen in the experimental studies. However, recent studies have shown that ACE inhibitors, especially captopril, could give protective effects on kidneys in insulin-dependent diabetics with nephropathy. Captopril also reduces the risk of death, dialysis, and kidney transplantation in those patients.Key words: diabetes mellitus -- kidney disease -- ACE inhibitors -- albuminuria -- kidney preservation
The Effects of new T cell line derived lymphokines on B cell activation Sofia Mubarika Haryana Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.579 KB)

Abstract

Telah diketahui bahwa untuk aktivasi limfosit B diperlukan faktor pertumbuhan yang disebut limfokin atau sitokin. Banyak bukti menunjukkan bahwa limfosit T yang teraktivasi menghasilkan substansi yang dapat memacu proliferasi atau diferensiasi limfosit B. Dewasa ini telah dapat diidentifikasi berbagai limfokin yang disebut sebagai interleukin (IL) seperti IL-2, IL-3, IL-4, IL-5, IL-6 dan IL-7 yang ikut berperan dalam aktivasi sel B. Namun, mekanisme aktivasi sel B oleh limfokin sampai sekarang masih terus berkembang dan dipelajari. Untuk dapat lebih memahami aktivasi sel B, dalam penelitian ini telah dilakukan pembuatan suatu T cell line, dan telah dikarakterisasi, yakni menunjukkan fenotipe Thyl+, Lyt1+2+, Dalam penelitian ini berbagai aktivator poliklonal lipopolysaccharide (LPS), ConA, dextran sulfat dipakai untuk memacu T cell line menghasilkan limfokin. Aktivitas limfokin pada supernatannya kemudian dianalisis dengan menggunakan blastogenesis assay dengan 3H-thymidine dan hemolytic plaque assay.Hash penelitian menunjukkan bahwa T cell line menghasilkan faktor pertumbuhan yang memacu proliferasi sel B (B cell growth factor II = BCGF II atau IL-5) dan faktor yang memacu sel B untuk berdiferensiasi (B cell differentiating factor = BCDF atau IL- 6) dan menghasilkan igM dan IgG.Key words : blastogenesis assay -- hemolitic plaque assay -- B cell activation -- T cell line -- lymphokines
Hemophilia with intracranial bleeding - A case report Sunarto Sunarto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.636 KB)

Abstract

A hemophiliac boy was hospitalized due to head injury in the previous sixteen days. On admission the' consciousness decreased and visus of both eyes was 1/on. CT scan showed blood clot in the area of dorsomedial of medial cerebral arteria and sign of hydrocephalus. A surgical procedure could not be taken due to lack of facilities (antihemophilic factor supply and assay of factor VIII activity). The patient was given 6 cryoprecipitate everyday and supportive treatment as indicated. Fortunately the patient recovered, no neurologic complication occurred except the visus of the right eye that was still 1/oo and the hydrocephalus decreased.Key words : hemophilia - laboratory diagnostics - head injury - hydrocephalus and decreased visus - cryoprecipitate
The Effect of budesonide on lymphoid and non-lymphoid cell profiles, and la-antigen expression in rats with experimental colitis Marsetyawan HNE Soesatyo Marsetyawan HNE Soesatyo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.611 KB)

Abstract

Kortikosteroid merupakan obat yang efektif terhadap peradangan, seperti penyakit peradangan usus kronis. Penelitian ini bertujuan untuk mengetahui pengaruh pamberian kortikosteroid lokal, yakni budesonide colitis experimental pada tikus, khususnya pengaruhnya terhadap populasi sel limfoid dan nonlimfoid, serta ekspresi antigen-la (molekul major histocompatibility complex (MHC) kias II). Di camping itu diteliti pula peran molekul-molekul adhesi, seperti intercellular adhesion molecules (iCAM-1) dan lymphocytes function associated antigen (LFA-1) setelah pemberian obat tersebut.Model colitis dibuat dengan pemberian secara intracolon zat hapten: 2,4,6-trinitrobenzene sulphonlc acid (TNBS) dalam etanol, berdasarkan metode standar. Obat budesonide diberikan deism deals tunggal dan ganda sebanyak 0,25 ml larutan 104 M secara lokal ke dalam colon dengan menggunakan kateter. Populasi sal limfoid dan nonlimfoid di sepanjang mukosa dan submukosa, termasuk plak Payer (PP) dan jaringan limfoid di daerah proksimal colon diperiksa dengan teknik imunositokimiawi. Ekspresi MHC Ida* ii dan molekul adhesi diteliti menggunakan panel antibodi monoklonal (AbMo).Dosis ganda budesonide (3x) *angst efektif untuk mengobati colitis akut; ini dapat diamati dad gejala-gejala klinis yang menghilang. Pemeriksaan histologik menunjukkan penurunan nyata jumlah subpopulasi makrofag, seperti sel EDI% ED2* dan ED3+. Intensitas ekspresi la dan ekspresi ICAM-1, LFA-1 mengurang, sdang lainnyaedangkan migrasi sal neutrofil dan sel radang lainnya menghilang.Key words : inflammatory bowel disease -- TNBS colitis -- budesonide-lymphoid and non-lymphoid cell populations -- MHC class II expression -- adhesion molecules.

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