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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 15 Documents

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Search results for , issue "Vol 50, No 4 (2018)" : 15 Documents clear

Correlation between specific rubella immunoglobulin G (Ig G) and response evoked brainstem audiometry examination on confirmed congenital rubella syndrome (CRS) patients Rianto, Bambang Udji Djoko; Pratomo, Akmaluddin Agung; Prasetyo, Ashadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Congenital rubella syndrome (CRS) is a health problem that can cause deaf child. Itsdiagnosis is based on clinical manifestation, specific rubella Immunoglobulin M (IgM) andIgG examination. The aim of this study was to evaluate the correlation between the specificrubella IgG level and the brainstem evoked response audiometry wave V. This was across sectional study involving congenital rubella syndrome (CRS) child patients at Dr.Sardjito General Hospital, Yogyakarta from 2011 to December 2016. The inclusion criteriawere 1) aged less than 24 months; 2) wave V of brainstem evoked response audiometryexamination has been identified. The exclusion criteria were 1) the patient’s parents refusedto participate and 2) incomplete data. Based on α: 5%, β: 20% and coefficient correlationestimation 0.04, minimal sample size were 47 subjects. All of subjects was underwentspecific rubella IgG examination. Statistical analysis by using Spearman`s correlation test.Wave V at the level <25 dB were found in 2 patients (4.3%), 26-40 dB results in 3 (6.4%)patients, 41-60 dB in 1 (2.1%) patient, 61-80dB was found in 9 (19.1%) patients and > 81db in 32 (68.1%) patients. A significant positive linear correlation between IgG levels (4-400 IU/mL) and wave V of brainstem evoked response audiometry was reported (p<0.05;r = 0.432). In conclusion, there is a positive correlation between specific rubella IgG leveland brainstem evoked response audiometry test results.

The role of isoflavon in reducing blood glucose levels Prasetyastuti, .
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Plants provide excellent sources of medicinal compounds. Over 5000 flavonoids havebeen isolated and identified from plant sources. Flavonoids are polyphenolic compoundsmainly recognized as pigments which produce the colors found in flowers, fruit and leaves.Dietary polyphenols are commonly found in plants and are abundant in fruit, vegetables,chocolate, and nuts. These polyphenols also found in beverages including tea, coffee,wine, and soy milk. Flavonoids are known to lower glucose levels, decrease cholesteroland triglycerides, and increase liver enzyme levels most likely by stimulating pancreaticinsulin release. By activating cell-signaling pathways involved in controlling blood glucoselevels, flavonoids aid in carbohydrate digestion, and adipose deposition. Flavonoidscontaining isoflavones, flavanols, flavanones, flavonols, flavones, and anthocyaninshave been studied from clinic study until pre clinic study. However, not all of the studiesconducted had adequate results. Isoflavone is one of the potential flavonoids that still canbe explored for future research. Research on isoflavone supplementation in animals andhumans in reducing blood glucose levels is still limited. Short-term supplementation didnot improve blood glucose levels in postmenopausal women, while other studies suggestthat the long-term isoflavone supplementation can reduce postmenopausal blood glucoselevels. These findings found that isoflavone is a component of flavonoids that have potencyin reducing blood glucose levels.

Patient prognosis after relief of obstruction R Danarto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTObstructive uropathy may lead to irreversible kidney damage. The insertion of ureteral stent is one of procedure to relief obstruction and prevent further kidney damaged, septicemia as well as urosepsis. A study to evaluate the predictive factor of urinary diversion in improving kidney function is needed. This study aimed to assess prognosis factors post-relief of obstruction in patients with uropathic obstruction focusing on kidney function recovery. This was a retrospective cohort study conducted at An-Nur Private Hospital in Yogyakarta Special Region, Indonesia using the medical record of patients with obstructive uropathy whom underwent DJ Stent insertion from a period of 2011 to 2015. The data of clinical assessment, laboratory and radiologic examination data were collected and analyzed using Chi square or student t test. A total 59 patients with obstructive uropathy aged around 50.9 years whom underwent DJ Stent were involved in this study. The results showed that release of obstruction with the installation of ureteral stents was significantly able to improve kidney function. The duration of obstruction, etiology, and hemoglobin (Hb) levels were associated with creatinine levels, whereas gender, urinary tract infection (UTI) and comorbid were not. In conclusion, prognosis factors of postrelief obstuction are duration of obstruction, etiology of obstruction and Hb levels before the release of obstruction

Assessment of the normal anal position index (API) of Indonesian neonates Yudi Suryana; Akhmad Makhmudi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The existing anal position index (API) data appear to vary among the ethnic differences.Until now, however, the normal API has not been studied in Indonesia. This study aimedto determine the normal value of the API in Indonesian newborns This cross-sectionalstudy was conducted on 62 neonates (29 males and 33 females) without any malformationat Pediatric Surgery Division, Department of Surgery, Dr. Sardjito General Hospital,Yogyakarta during the period September to October 2012. The position of the anus wasnumerically defined by the API, which is the ratio of anus-fourchette distance in females and anus-scrotum distance in males to the distance between coccyx and fourchette/scrotum. To make correct measurements, transparent adhesive tape was used longitudinally on midperinum in a way that it covered the anus. Then fourchette/scrotum, anus center and the lower margin of coccyx were marked on it. Distances marked on each tape were then measured with the standard ruler. Relationship between API and other parameters were analyzed. The API values were 0.46±0.05 (95% CI: 0.44–0.48) for newborn males and 0.37±0.07 (95% CI: 0.35–0.39) for newborn females. The difference of API between males and females was significant (p = 0.000). API had no significant correlation with gestational age (p = 0.350) and birth weight (p = 0.650). Our data suggested the API, which is sex dependent, provides a reliable parameter for determining the position of the anus. API has no correlation with gestational age and birth weight. The determination of normal API in older infants is required to determine whether the API is affected by age.

The effect of human saliva compared to Aloe vera on wound healing of 2nd degree burn injury in animal models Budi Cahyono Putro; Ishandono Dachlan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Burn injury is one of the common causes of injury that has relatively high morbidity and mortality. Several studies using herbal and traditional medicine from different countries have been documented in burn injury management. Human saliva that contains antibacterial, antifungal, antiviral and analgesic components as well as growth factors can induce re-epithelialization process in 2nd degree burn injury. Whereas, Aloe vera that influence a physiological moist condition was proven can induce re-epithelialization process lead to faster wound healing. This study aimed to compare topical application of human saliva and A. vera on wound healing process of 2nd degree burn injury. This was an experimental study using post-test only control group design using 27 white rats (Rattus novergicus) of Sprague Dawley strain divided into 3 groups with 9 rats in each group. Group I were applied 1 mL of human saliva, Group II were applied A. vera and Group III as control were applied NaCl. Change in body weight and macroscopic clinical assessment were observed every day for 14 days, whereas histological examination was observed on day 14. The data were presented as mean ± standard error of the mean (SEM) and analyzed using one-way analysis of variance (Anova). The result showed that the wound healing process at each treatment showed different level. The human saliva application tended to show faster wound healing process of 2nd degree burn injury compare with A. vera or NaCl (p

Differentiation of body fat composition between skinfold caliper (SKF) and bioelectrical impedance analysis (BIA) methods among professors Nicole Ngo Zhe Yiin; Leonardo Lubis; Dimas Erlangga Luftimas
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Body fat composition reflected by body fat percentage (BF%) is one of the important components in disease risk evaluation. Among the methods available to measure BF%, skinfold calipers (SKF) and bioelectrical impedance analysis (BIA) are the most commonly used. The study was conducted to evaluate the difference in body fat composition measurement between SKF and BIA methods among professors. This study analyzed secondary data from the health evaluation of Universitas Padjadjaran (UNPAD) professors. This study involved 72 professors (50 male, 22 female) after fitted into the inclusion and exclusion criteria. BF% was measured among UNPAD professors using SKF and BIA. After obtaining agreement by Bland-Altman Plot, the data were analyzed by muliple paired-t test according to gender, physical activity level and body mass index (BMI) categories. The study showed no significant difference in BF% between SKF and BIA in overall population, between gender and physical activity level (p>0.05). For BMI, the only category that showed significant difference in BF% between the 2 methods was obese I group (p=0.001). In conclusion, there is no significant difference in BF% between SKF and BIA methods according to gender, physical activity levels and BMI categories except for obese I group.

Relationship Between Pfmdr1 Gene Polymorphisms with Therapeutic Respons of Artesunate-Amodiaquine in Uncomplicated Falciparum Malaria Patients in Puskesmas Hanura, Pesawaran Regency, Lampung Carolia, Novita; Mustofa, .; Kristin, Erna
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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ABSTRACTBackground: The failure of artesunate-amodiaquine therapy in chloroquine resistant patients have been reported in several areas in Indonesia. One of the unexpected resistance mechanisms is associated with pfmdr1 gene polymorphisms. This study determine the frequency of pfmdr-1 polymorphisms and examine the relationship of pfmdr1 gene polymorphism with therapeutic responses to artesunate-amodiaquine therapy in uncomplicated falciparum malaria patients in Puskesmas Hanura, Pesawaran Regency, Lampung Methods: This study was conducted in accordance with the WHO protocol for anti-malarial drug efficacy test in uncomplicated falciparum malaria. Combination therapy used artesunate-amodiaquine and primaquine. Therapeutical response and Parasite Clearance Time was observed during 28 days of observation. Diagnosis polymorphism by PCR-RFLP technique used the patient's blood (EDTA and filter paper) were taken a tH0.Results: There were 63(85.1%) patients of Adequate Clinical and Parasitological Response, Early Treatment Failure were 5 (6.8%) people, Late Clinical And Parasitological Failure 2 (2.7 %) people, and Late Parasitological Failure 4 (5.4%) people. Seven (13.5%) patients were found with N86Y pfmdr1 gene polymorphism with mixed type (mutant type and wild-type) in which 1 (9.1%) people had failed therapy and 6 (14.6%) people were cured. There was no association between pfmdr1 gene polymorphism with artesunate-amodiaquine failed therapy (RR: 0.64, 95% CI0.97-4.27, p: 1.000). Parasite Clearance Time on pfmdr1 polymorphism group was 2.14 ± 1.21 days and 1.79 ± 1.06 days in group without pfmdr1 polymorphisms. There was no significant difference between both groups (p =0.434).Conclusions:The frequency of pfmdr1 N86Y polymorphism in Puskesmas Hanura, Pesawaran Regency, Lampung was 13,5%. There was no significant difference between the rate of treatment failure patients with pfmdr1 N86Y polimorphism compared with patients without pfmdr1 N86Y polymorphism. There was no significant difference between Parasite Clearance Time patients with pfmdr1 N86Y polymorphism compared with patients without pfmdr1 N86Y polymorphism.Keywords: falciparum malaria, artesunat-amodiakuin, polymorphism, pfmdr1

SCN1A exon 26 variants in epilepsy and migraine patients Nova Dian Lestari; Endang Mutiawati; Ahmad Hamim Sadewa; Hasan Sjahrir; . Syahrul; Dessy Rakhmawati Emril; . Harapan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Epilepsy and migraine are common neurological diseases in many populations. Mutation of the voltage gated natrium channel Nav1.1 (SCN1A) are important causes of different genetic epilepsies and can also cause familial hemiplegic migraine (FHM-III). This study aimed to identify SCN1A gene variation in patients with epilepsy and common migraine. Gene variation analysis of exon 26 of the SCN1A gene was carried out in 33 patients with epilepsy, 33 patients with migraine and 30 control individuals from Neurology Polyclinic at Dr. Zainoel Abidin General Hospital, Banda Aceh. The PCR and direct sequencing methods were performed in this study. SCN1A gene variations were identified in two epilepsy patients. These gene variations located at exon 26 were four silent mutations in patient E27 at position A4440T (Leu1480Leu), T4443C (Leu1481Leu), A5046G (Leu1682Leu) and C5121T (Asp1707Asp). One silent mutation in patient E30 at position G5505A (Glu1835Glu). None of these gene variations were identified in controls and patients with common migraine in this study. This study has identified 5 genetic variations of SCN1A in patients with epilepsy but not in common migraine. The mechanism and relationship between these variants and epilepsy need to be clarified.

Expression of circulating miR-200c and vascular endothelial growth factor-A (VEGF-A) mRNA as potential biomarker in human hepatocellular carcinoma Nanda Qoriansas; Dede Renovaldi; Juwita Raditya; Puji Lestari; Nur Signa Gumilas; . Suharno; Didik Setyo Heriyanto; Neneng Ratnasari; Sofia Mubarika H
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Hepatocellular carcinoma (HCC) is a common liver disease that causes significant publichealth problems throughout the world, including in Indonesia. The HCC is the six mostcommon cancers and second cancer-related deaths among men in the world. Recentlyit was reported that the microRNA is an important player in hepatocarcinogenesis. Theexpression of MiRNA-200c is often regulated in primary HCC and HCC cell lines. Vascularendothelial growth factor-A (VEGF-A) is a regulator of angiogenesis that has been reportedas miR-200c target gene. This study was conducted to measure expression levels in miR-200c and mRNAVEGF-A and their potential role as biomarkers at HCC. A total of 36HCC patients and 36 healthy subjects were included in this study. The relative expressionof miRNA-200c and mRNA VEGF-A was quantified using reverse transcription real timequantitative PCR (qRT PCR). Relative expression was calculated using . Unpaired t-testwas used to compare the expression levels of circulating miRNA-200c and mRNA VEGF-Ain HCC patients and healthy subjects. Pearson test was used to determine correlationbetween circulating miR-200c expression and mRNA VEGF-A expression levels. Theexpression levels of circulating miR-200c in HCC patients were lower compared to healthysubjects although it was not significant (p = 0.258). Conversely, the expression levelsof circulating mRNA VEGF-A in HCC patients were significantly higher compared tohealthy subjects (p = 0.001). The relative expression levels of circulating miR-200c werenegatively correlated with mRNA VEGF-A in HCC patients. In conclusion, the expressionlevels of mRNA VEGF-A in HCC patients are significantly deregulated in compared tothat in healthy subjects. Negative correlation between circulating miRN-200c and mRNAVEGF-A expression levels are reported in HCC patients.

mRNA expression of CYP17A1, CYP11A1, CYP19A1, HSD3B1 and AKR1C2 in metastatic and non-metastatic prostate cancer patients Indrawarman Soerohardjo; Muhammad Puteh Mauny; Alharsya Franklyn Ruckle; Ahmad Zulfan; Raden Danarto; Didik Setyo Heriyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The progression of prostate cancer (PCa) mainly occurs caused by androgens. There is a linkbetween intratumoral steroidogenesis and castration-resistant prostate cancer. This studyaimed to determine the mRNA expression of various steroidogenic enzymes (CYP17A1,CYP11A1, CYP19A1, HSD3B1, and AKR1C2) in metastatic and non-metastatic prostatecancer patients. This study was conducted at the Anatomical Pathology Laboratory andUrologi Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing,Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta from September-November 2017. Samples were taken from 30 paraffin blocks with adenocarcinoma ofprostate, stained with hematoxylin-eosin (HE) and then classified into metastatic and nonmetastaticgroups. Samples then underwent deparaffinization procedure and examinationof mRNA expression of CYP17A1, CYP11A1, CYP19A1, HSD3B1, AKR1C2 genes usingReal-Time PCR. The mean mRNA expressions of CYP11A1, CYP17A1, CYP19A1,HSD3B1, and AKR1C2 genes in the metastatic adenocarcinoma prostate group were 7.08,10.11, 3.94, 4.84 and 3.58, respectively. In the non-metastatic group, the mean mRNAexpressions of CYP11A1, CYP17A1, CYP19A1, HSD3B1, and AKR1C2 genes were 4.62,9.45, 3.46, 2.68 and 4.92, respectively. The mean of mRNA expression of CYP11A1,CYP17A1, CYP19A1, and HSD3B1 genes were higher in the metastatic group than nonmetastaticadenocarcinoma prostate group. However, it was not statistically significant(p>0.05). The highest mRNA expression of steroidogenic enzymes was the CYP17A1gene. In conclusion, the mRNA expressions of CYP17A1, CYP11A1, CYP19A1, HSD3B1were higher in the metastatic prostate cancer patients compared to that in non-metastaticprostate cancer patients but statistically not significant.

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