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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 16 Documents
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Search results for , issue "Vol 14, No 3 (2009): October 2009" : 16 Documents clear
Seasonal Activity of Metabolic Enzymes of Littorina littorea (Gastropoda: Mollusca) G. J. Fontje Kaligis
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2578

Abstract

Aktivitas musiman enzim pyruvate kinase (PK), dan citrate synthase (CS) pada otot kaki siput, Littorina littorea (Gastropoda, Littorinidae) telah diteliti. Enzim-enzim ini berperan sebagai enzim kunci pada rangkaian metabolisme (metabolic pathways). Perbedaan yang signifikan terlihat pada aktivitas adalah CS dan PK sepanjang musim. Aktivitas spesifik dari CS bervariasi antara 0,04 dan 0,4 μg berat basah dan PK antara 0,1 dan 1,7 μg berat basah. Aktivitas enzim tersebut lebih tinggi pada musim dingin dibandingkan dengan musim panas. Hubungan negatif antara suhu dan aktivitas enzim terdeteksi dalam penelitian ini. Terdapat perbedaan yang nyata antara suhu air dan aktivitas enzim CS, dan suhu air dan udara pada enzim PK (P<0.05). Teramati tendensi yang sama untuk prosentasi perubahan pada aktivitas enzim PK dan CS pada bulan pertama pengamatan dan bulan berikutnya. Hasil penelitian setuju dengan hipotesis yang mengatakan bahwa terdapat kemungkinan perubahan aktivitas enzim CS dan PK selama siklus musiman. Fenomena ini dibahas dalam pembahasan tulisan ini.
Expression and Characterization of Recombinant Protein of J-SU pGEX either by Single or Double Cell Lysis Endang Tri Margawati; Muhamad Ridwan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2579

Abstract

Penelitian ini dimaksudkan untuk optimasi produk protein rekombinan Superficial Unit dari virus Jembrana (JSU) yang dieksperikan melalui pemecahan sel secara tunggal dan ganda dengan sistem pGEX dalam skala flask 100ml media kultur. Dua metode pemecahan sel yang digunakan yaitu Freeze and Thaw (FT) sebagai pemecahan tunggal dan gabungan FT dan Sonikasi sebagai pemecahan ganda. Sel inang (E. Coli pembawa konstruk JSU pGEX) ditumbuhkan dengan induksi IPTG pada 37oC dengan pengocok berkecepatan 200rpm sampai mencapai kepadatan sel 0,8. Sel atau pelet dikoleksi dengan sentrifugasi, pelet dipecah dengan 2 perlakuan pemecahan sel tunggal dan ganda. Hasil pemecahan sel disentrifugasi untuk dikoleksi peletnya sebagai inclusion body. Solubilisasi dilakukan terhadap inclusion body dengan solubilisasi buffer dan diperoleh substrat protein JSU kemudian dimurnikan melalui Gluthation sepharose 4B (500μl resin) dengan metode batch capture. Hasil karakterisasi dengan SDS PAGE dan Western Blotting menunjukkan ukuran protein JSU pGEX yang tepat yaitu 60kDa pada kedua sistem pemecahan sel. Namun demikian, pemecahan sel secara tunggal menghasilkan protein murni JSU pGEX lebih besar (0.812ng/ul) dibanding pemecahan sel secara ganda (0.486ng/ul). Dari penelitian ini dapat disimpulkan bahwa protein rekombinan JSU pGEX terekspresi lebih baik dengan metode pemecahan sel Freeze and Thaw.
Analisis Ekspresi Gen Selenometil Transferase pada Isolat Bakteri Termofilik Geobacillus 20K dan Thermomicrobium 14Ka sebagai Sumber Selenoprotein Evi Triana; Novik Nurhidayat; Sri Hartin Rahayu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2580

Abstract

Selenium is a trace element that has essential nutrition value for human. Besides its nutritional value, it has important health benefits, including being a cancer chemoprotective agent. Methylated form of selenium is the most effective compound against cancer cells. Selenomethyl transferase (SMT) is responsible for methylating of selenium. This enzyme is coded by selenomethyl transferase (smt) gene which was found only from selenium accumulator plant, Astragalus bisulcatus. Thermophilic bacteria Thermomicrobium 14Ka and Geobacillus 20K have ability to accumulate selenium as well and potential in fighting cancer cells. Therefore a study to determine smt gene and its expression in both bacteria had been conducted in order to develop natural product of seleno-metilselenosistein for cancer treatment. The result showed that Thermomicrobium 14Ka and Geobacillus 20K have putative smt (selenomethyl transferase) gene, and such gene was expressed at different intensity. Geobacillus 20k expressed smt gene at higher intensity than Thermomicrobium 14k. Therefore, it is presumable that Geobacillus has a significant role in cancer remedy, meanwhile Thermomicrobium plays an essential role as cancer protective agent.
Karakterisasi α-Amilase dari Aspergillus versicolor 3a1 yang Diproduksi pada Media Limbah Cair Tapioka Fitratul Aini; Nisa Rachmania Mubarik; Lisdar A. Manaf
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2581

Abstract

The aim of this experiment was to characterize A. versicolor 3a1 α-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 α-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of α-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 α-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. α-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 α-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 α-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 α-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 -amylase on cassava waste media. Crude α-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.
Uji Patogenisitas Isolat Bakteri Indigenous (Bacillus thuringiensis) terhadap Serangga Hama Kubis (Crocidolomia binotalis Zell) Christina L. Salaki; Jesmandt Situmorang; Langkah Sembiring; Niken Handayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2582

Abstract

Pathogenicity of 34 indigenous B. thuringiensis isolates against C. binotalis were determined. The pathogenicity test was conducted by using leaf dipped method with various spore concentrations. Third instar larvae of C. binotalis were used as insect test. Mortality data of test larvae were used to determine the pathogenicity of the isolates in terms of 72 hours LC50 by using probit analysis. The results of experiments showed YPPA 1. was the most pathogenic isolate, producing 72 hours LC50 = 9.5 x 103 spore.ml-1 with LT50 (1.5 x 107 spore.ml-1) of 24.6 hours while the ACH 2.3 was found to be the least pathogenic isolate with 72 hours LC50 = 2.3 x 106 spore.ml-1 and LT50 (1.5 x 107 spoore.ml-1) of 40.7 hours. The shortest LT50 (1.5 x 107 spore.ml-1 was found to be 18.2 hours produced by TUS.1 with 72 hours LC50 = 3.9 x 105 spore.ml-1 whereas the longest LT50 (1.5 x 107 spore.ml-1) was found tobe 83.2 hours produced by the SLK 4.1 with 72 hours LC50 = 3.1 x 104 spore.ml-1. Therefore, it can be concluded that both YPPA.1 and TUS.1 isolates are potential candidate to be developed for biological control agent.
High Prevalence Level of Avian Malaria in the Wild Population of the Java Sparrow Pramana Yuda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2583

Abstract

Java sparrow (Padda oryzativa) is anendemic bird to Java and Bali. It used to be avery common bird, but due to over exploitationthe bird has declined and been classified asVulnerable (BirdLife International, 2001). InIndonesia bird-keeping is a popular pastime,with deep cultural roots (Jepson and Ladle,2005). It is widely assumed that the hobbynegatively affects wild populations of commonas well as threatened birds (Jepson and Ladle,2005; Nash, 1994), such as Java sparrow.

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