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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Al-Kimia
Novik Nurhidayat
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Veterinary

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Aktivitas urikase yang dihasilkan dari berbagai sel lactobacillus plantarum dan parameter kinetikanya Dyah Iswantini; Novik Nurhidayat; . Trivadila; Eka Mardiah
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 3 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Uric acid concentration could be determined by spectrophotometry method. Uric acid was oxidized into allatonin in the presence of uricase and calculated by measuring the decrease of uric acid absorbance at 293 nm. These uricase were obtained from cells of Lactobacillus plantarum. L. plantarum K. Mar. E was isolated from passion fruit skin and L. plantarum Mgs. Psmb and Mgs. Bst from mangosteen. This research was conducted to observe the activity and kinetics of uricase from various cells of L. plantarum by spectrophotometric method. The plate assay method indicated that L. plantarum produced uricase, based on the clear zone about 0,2 mm on glucose yeast peptone medium contained 0,2% uric acid. The optimum condition of uricase activity from the three different sources occured in physiological condition. Uricase activity generated from cells of L. p/antarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 0,1073; 0,0867; and 0,0842 U/ml, respectively. The kinetic parameters for uricase, determined with uric acid as the substrate. Vmax produced by L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 1,3635; 0,0316; and 0,0418 U/ml of bacterial culture, respectively and KM 0,1541; 0,0061; and0,0054 mM, respectively. Uricase activity in various bacterial cells of L. plantarum was stable until the second day.
Penentuan kinetika urikase dari sel bacillus subtilis, B. megaterium, dan B. cereus Dyah Iswantini; Novik Nurhidayat; . Trivadila; Adayani Nurjayanti
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Uric acid concentration could be determined based on the oxidation of uric acid into alantoin in the presence of uricase. Determination of uric acid concentration is needed to diagnose the occurrence of kidney disease in gout patients. The aim of the research was to study the kinetics of uricase in Bacillus subtilis, B. megaterium, and B. cereus cells using spectrophotometry method by measurement the decrease of uric acid absorbance at 293 nm. The optimum conditions of uricase activity from the three bacterial cells occured in physiological conditions and uricase activity was stable until the second day. The values of maximum velocity (Vmax) for B. subtilis, B. megaterium, and B. cereuswere 0.1642, 0.0824, and 0.0412 mM/min, respectively. The values of Michaelis-Menten constant (Km) for B. subtilis, B. megaterium and B. cereus were 0.0003, 0.0036, and 0.0036 mM, respectively. The value of catalytic constant (kkat) for B. subtilis, B. megaterium, and B. cereus were 410.5000, 171.6667, and 257.5000 mM/min ml 00=1, respectively. Based on these results, among all bacteria tested, the highest of uricase binding with substrate in B. substilis cells was observed because of the smallest of Km value and greatest of Vmax and kkat .
Seleksi Sel Bakteri Dari Minyak Bumi Sebagai Molekul Pengenal Dalam Biosensor Benzena Alfiah Alif; Dyah Iswantini; Henny Purwaningsih; Novik Nurhidayat; Amalyah Febryanti
Al-Kimia Vol 9 No 2 (2021): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v9i2.24378

Abstract

Benzene is one of the harmful compounds which can affect both healthcare and environmental quality. Conventionally the effort of detecting this compound still requires several sample pre-treatments, contributing to a long analysis time and sophisticated instrumentation. Therefore, this study was aimed to determine the potency of bacteria as the bioreceptor for detecting benzene electrochemically. The bacteria isolate was immobilized on the working electrode surface. Four bacteria isolates from the petroleum sample were evaluated. The results showed that isolate II produced high oxidation and reduction peak currents as much as 150 µA and -490 µA respectively. The selected bacteria showed characteristics to Pseudomonas sp. physiologically. Since the bacteria can degrade benzene, thus hypothetically it can produce benzene dioxygenase. Through the catechol formation, 3 mM benzene produced 108.7 µA after 11.4 s from the starting scan. This result suggested that the excreted enzyme from the selected bacteria could react with benzene enzymatically.
Seleksi Dan Identifikasi Lactobacillus Kandidat Probiotik Penurun Kolesterol Berdasarkan Analisis Sekuen 16s Rna Evi Triana; Novik Nurhidayat
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2537

Abstract

High fat and low fiber dietary pattern results in raising of blood cholesterol level over the normal level, namely hypercholesterolemia. Hypercholesterolemia might cause coronary disease and stroke. Blood cholesterol is able to be decreased by probiotic supplement. Lactobacillus is one of the probiotics that were well known and taken advantages. However its role as cholesterol lowering agent was less known. Therefore, screening and identification of Lactobacillus isolates which were candidates of probiotic have been carried out. Isolates Mar 8, Lac 3 and 7 p have been selected as Lactobacillus candidates for cholesterol lowering probiotic. Those isolates met criteria for cholesterol lowering probiotics. Furthermore, they have been conducted to confirm their identity as Lactobacillus. 16S RNA sequences analysis by BLAST analysis against reference strains within DNA Data Bank of Japan (DDBJ) have been carried on. Results showed that sequences of Lactobacillus Mar 8 was 100% homology with Lactobacillus plantarum, Lac 3 was 100% homology with Lactobacillus paracasei and 7 p was 99% homology with Lactobacillus plantarum. It was concluded that the three isolates were selected as candidates for cholesterol lowering probiotics. Both of them, Mar 8 and 7 p, are Lactobacillus plantarum. Another one, Lac 3 is Lactobacillus paracasei.
Analisis Ekspresi Gen Selenometil Transferase pada Isolat Bakteri Termofilik Geobacillus 20K dan Thermomicrobium 14Ka sebagai Sumber Selenoprotein Evi Triana; Novik Nurhidayat; Sri Hartin Rahayu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2580

Abstract

Selenium is a trace element that has essential nutrition value for human. Besides its nutritional value, it has important health benefits, including being a cancer chemoprotective agent. Methylated form of selenium is the most effective compound against cancer cells. Selenomethyl transferase (SMT) is responsible for methylating of selenium. This enzyme is coded by selenomethyl transferase (smt) gene which was found only from selenium accumulator plant, Astragalus bisulcatus. Thermophilic bacteria Thermomicrobium 14Ka and Geobacillus 20K have ability to accumulate selenium as well and potential in fighting cancer cells. Therefore a study to determine smt gene and its expression in both bacteria had been conducted in order to develop natural product of seleno-metilselenosistein for cancer treatment. The result showed that Thermomicrobium 14Ka and Geobacillus 20K have putative smt (selenomethyl transferase) gene, and such gene was expressed at different intensity. Geobacillus 20k expressed smt gene at higher intensity than Thermomicrobium 14k. Therefore, it is presumable that Geobacillus has a significant role in cancer remedy, meanwhile Thermomicrobium plays an essential role as cancer protective agent.
Isolation and Molecular Identification of Nitrate-Reducing Bacteria from Swiftlet Houses in Sumedang, West Java Ningrum, Siti Gusti; Novik Nurhidayat; Titin Yulinery; Evi Triana; Olan Rahayu Puji Astuti Nussa; Ady Kurnianto
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.1-7

Abstract

Edible bird‘s nest is an important export commodity that is currently of concern to the Indonesian government. One of the requirements for exporting edible bird’s nest to the People's Republic of China is that this product must meet the requirements for nitrite content in the product below 30 ppm. This nitrite contamination can be obtained from the results of nitrogen metabolism from nitrifying bacteria. However, information on nitrite-reducing bacteria in swiftlet houses has never been reported and is urgent in controlling nitrifying bacteria in swiftlet houses. The presence of nitrite-reducing bacteria needs to be identified to prove the presence of these bacteria in swiftlet houses that have the potential to contribute to nitrite contamination in edible bird’s nest. This study aims to isolate nitrate-reducing bacteria in an effort to control nitrite using bacteriophages in the future. This study targeted nitrate-reducing bacteria collected from environmental samples (waste, feces, pond water, artificial pond water, soil, swiftlet eggshells, white edible bird’s nest (Aerodramus fuciphagus), black bird’s nest (Aerodramus maximus)) (n=40) from two different swiftlet houses in Sumedang, West Java, Indonesia. All isolates collected were subjected to a series of microbiological tests, phenotypic characterization (Gram staining, morphology, sugar fermentation ability, enzymes, etc.) and genotyping by PCR amplification and 16S rDNA gene sequencing. Raw sequencing data were analyzed using DNASTAR® software for DNA sequence alignment and phylogenetic tree construction. In the present work, four bacteria species were identified, including Priestia megaterium, Pseudomonas putida, Stenotrophomonas maltophilia, and Proteus terrae. To our knowledge, this study is the first report of nitrate-reducing bacteria isolated from birdhouses.
Co-Authors . TRIVADILA Adayani Nurjayanti ady kurnianto Alif, Alfiah Amalyah Febryanti Dyah Iswantini Eka Mardiah Evi Triana Henny Purwaningsih Olan Rahayu Puji Astuti Nussa Siti Gusti Ningrum, Siti Gusti Sri Hartin Rahayu Titin Yulinery