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All Journal ACTA VETERINARIA INDONESIANA Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI Jurnal Kimia Terapan Indonesia JIEB : Jurnal Ilmiah Ekonomi Bisnis
Evi Triana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Materials Science & Nanotechnology Mathematics Veterinary Other

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Seleksi Dan Identifikasi Lactobacillus Kandidat Probiotik Penurun Kolesterol Berdasarkan Analisis Sekuen 16s Rna Evi Triana; Novik Nurhidayat
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2537

Abstract

High fat and low fiber dietary pattern results in raising of blood cholesterol level over the normal level, namely hypercholesterolemia. Hypercholesterolemia might cause coronary disease and stroke. Blood cholesterol is able to be decreased by probiotic supplement. Lactobacillus is one of the probiotics that were well known and taken advantages. However its role as cholesterol lowering agent was less known. Therefore, screening and identification of Lactobacillus isolates which were candidates of probiotic have been carried out. Isolates Mar 8, Lac 3 and 7 p have been selected as Lactobacillus candidates for cholesterol lowering probiotic. Those isolates met criteria for cholesterol lowering probiotics. Furthermore, they have been conducted to confirm their identity as Lactobacillus. 16S RNA sequences analysis by BLAST analysis against reference strains within DNA Data Bank of Japan (DDBJ) have been carried on. Results showed that sequences of Lactobacillus Mar 8 was 100% homology with Lactobacillus plantarum, Lac 3 was 100% homology with Lactobacillus paracasei and 7 p was 99% homology with Lactobacillus plantarum. It was concluded that the three isolates were selected as candidates for cholesterol lowering probiotics. Both of them, Mar 8 and 7 p, are Lactobacillus plantarum. Another one, Lac 3 is Lactobacillus paracasei.
Analisis Ekspresi Gen Selenometil Transferase pada Isolat Bakteri Termofilik Geobacillus 20K dan Thermomicrobium 14Ka sebagai Sumber Selenoprotein Evi Triana; Novik Nurhidayat; Sri Hartin Rahayu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2580

Abstract

Selenium is a trace element that has essential nutrition value for human. Besides its nutritional value, it has important health benefits, including being a cancer chemoprotective agent. Methylated form of selenium is the most effective compound against cancer cells. Selenomethyl transferase (SMT) is responsible for methylating of selenium. This enzyme is coded by selenomethyl transferase (smt) gene which was found only from selenium accumulator plant, Astragalus bisulcatus. Thermophilic bacteria Thermomicrobium 14Ka and Geobacillus 20K have ability to accumulate selenium as well and potential in fighting cancer cells. Therefore a study to determine smt gene and its expression in both bacteria had been conducted in order to develop natural product of seleno-metilselenosistein for cancer treatment. The result showed that Thermomicrobium 14Ka and Geobacillus 20K have putative smt (selenomethyl transferase) gene, and such gene was expressed at different intensity. Geobacillus 20k expressed smt gene at higher intensity than Thermomicrobium 14k. Therefore, it is presumable that Geobacillus has a significant role in cancer remedy, meanwhile Thermomicrobium plays an essential role as cancer protective agent.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) Joko Sulistyo; Yati Sudaryati Soeka; Evi Triana; Rostiati NR Napitupulu
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
Pemanfaatan Limbah Kulit Udang untuk Menghasilkan Enzim Kitinase dari Streptomyces macrosporeus InaCC A454 Yati Sudaryati Soeka; Evi Triana
Jurnal Kimia Terapan Indonesia Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.441 KB) | DOI: 10.14203/jkti.v18i01.43

Abstract

Kitinase (EC 3.2.1.14) adalah enzim yang dapat menghidrolisis ikatanpanjang polimer asetil-D-glukosamina (kitin) menjadi ikatan polimer kitinyang lebih pendek. Kitin banyak ditemukan pada cangkang hewaninvertebrata laut, terutama krustasea,serangga, moluska, dinding sel jamur.Limbah udang sangat penting sebagai sumber kitin. Penelitian ini bertujuanuntuk menentukan karakteristik enzim kitinase yang dihasilkan olehaktinomisetes asal P. Belitung dengan substrat kitin dari limbah udang,meliputi waktu inkubasi, pengaruh pH, suhu optimum serta stabilitas.Pengaruh beberapa ion-ion logam sebagai aktivator atau inhibitor terhadapaktivitas enzim kitinase diukur dengan spektrofotometer pada λ 584 nm.Strain aktinomisetes diidentifikasi secara molekuler berdasarkan 16S rDNAsekuen. Aktivitas kitinase secara kualitatif diperlihatkan dengan adanya zonabening di sekitar koloni pada media yang mengandung 1% koloidal kitin.Hasil menunjukkan bahwa aktivitas tertinggi pada waktu inkubasi lima hari,suhu 50ºC dan pH 8 berturut-turut sebesar 0,0304 U/mL, 0,0312 U/mL dan0,0421 U/mL. Pengaruh ion logam dalam bentuk kationdivalen danmonovalen masing-masing pada konsentrasi 1 mM diaktifkan olehkationdivalen CaCl2, MnCl2, CuCl2 dan kation monovalen KCl, NaCl.Identifikasi molekuler berdasarkan sekuen16S rDNA menunjukkan bahwaisolat aktinomisetes ini adalah Streptomyces macrosporeus. Strain ini telahdidaftarkan di kultur koleksi InaCC dengan nomor koleksi A 454. 
ANALISIS AKUNTABILITAS DAN TRANSPARANSI PENGELOLAAN DANA BANTUAN LANGSUNG TUNAI DANA DESA (BLT-DD) DI DESA MURUTUWU KABUPATEN BARITO TIMUR Evi Triana
Jurnal Ilmiah Ekonomi Bisnis Vol 9 No 2 (2023): JURNAL ILMIAH EKONOMI BISNIS
Publisher : Jurnal Ilmu Ekonomi dan Bisnis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35972/jieb.v9i2.1155

Abstract

Abstrak: Tujuan penelitian ini untuk mengetahui bagaimana akuntabilitas pengelolaan BLT DD di Desa Murutuwu Kabupaten Barito Timur. Jenis penelitian yang digunakan adalah jenis penelitian kualitatif dengan metode pendekatan deskriptif dan menggunakan metode observasi, wawancara, dan dokumentasi. Permasalahan yang ada pada Desa Murutuwu Kabupaten Barito Timur antara lain dalam pengelolaannya belum tepat sasaran, dalam penyaluran sempat terlambat, tidak adanya fasilitas papan informasi pada kantor desa dan tidak adanya sosialisasi kepada masyarakat..Hasil penelitian menunjukan bahwa pada Kantor Desa Murutuwu Kabupaten Barito Timur dalam pengelolaan BLT DD secara akuntabilitas telah sesuai dengan prosedur yang berlaku yaitu dengan membentuk tim pendataan kemudian melakukan pengumpulan data setelah itu melakukan proses verifikasi, setelah proses tersebut dilakuakan maka tim pendataan dapat menentukan orang-orang yang berhak atas penerimaan bantuan langsung tunai dan diberikan sesuai dengan ketentuan Peraturan Presiden RI Nomor 104 Tahun 2021. Pengelolaan BLT DD secara transparansi selama ini dalam proses pengelolaan masih kurang diperhatikan dalam keterbukaan terhadap masyarakat untuk pelaporan anggaran dan daftar nama penerima BLT tidak diberitahuan melalui papan informasi. Seharusnya dalam pengelolaan pemerintah desa menggunakan papan informasi sebagai sarana untuk memberitahukan nama penerima BLT DD dan memberitahukan anggaran yang telah tersalurkan serta desa seharunya mengikutsertakan partisipasi masyarakat dalam rapat khusus untuk menentukan nama calon penerima BLT DD. Kata kunci: akuntabilitas, transparansi, BLT DD
Isolation and Molecular Identification of Nitrate-Reducing Bacteria from Swiftlet Houses in Sumedang, West Java Ningrum, Siti Gusti; Novik Nurhidayat; Titin Yulinery; Evi Triana; Olan Rahayu Puji Astuti Nussa; Ady Kurnianto
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.1-7

Abstract

Edible bird‘s nest is an important export commodity that is currently of concern to the Indonesian government. One of the requirements for exporting edible bird’s nest to the People's Republic of China is that this product must meet the requirements for nitrite content in the product below 30 ppm. This nitrite contamination can be obtained from the results of nitrogen metabolism from nitrifying bacteria. However, information on nitrite-reducing bacteria in swiftlet houses has never been reported and is urgent in controlling nitrifying bacteria in swiftlet houses. The presence of nitrite-reducing bacteria needs to be identified to prove the presence of these bacteria in swiftlet houses that have the potential to contribute to nitrite contamination in edible bird’s nest. This study aims to isolate nitrate-reducing bacteria in an effort to control nitrite using bacteriophages in the future. This study targeted nitrate-reducing bacteria collected from environmental samples (waste, feces, pond water, artificial pond water, soil, swiftlet eggshells, white edible bird’s nest (Aerodramus fuciphagus), black bird’s nest (Aerodramus maximus)) (n=40) from two different swiftlet houses in Sumedang, West Java, Indonesia. All isolates collected were subjected to a series of microbiological tests, phenotypic characterization (Gram staining, morphology, sugar fermentation ability, enzymes, etc.) and genotyping by PCR amplification and 16S rDNA gene sequencing. Raw sequencing data were analyzed using DNASTAR® software for DNA sequence alignment and phylogenetic tree construction. In the present work, four bacteria species were identified, including Priestia megaterium, Pseudomonas putida, Stenotrophomonas maltophilia, and Proteus terrae. To our knowledge, this study is the first report of nitrate-reducing bacteria isolated from birdhouses.
Co-Authors ady kurnianto Joko Sulistyo Novik Nurhidayat Olan Rahayu Puji Astuti Nussa Rostiati NR Napitupulu Siti Gusti Ningrum, Siti Gusti Sri Hartin Rahayu Titin Yulinery Yati Sudaryati Soeka