cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Jurnal Biotek Medisiana Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 23015810     EISSN : 23548800     DOI : -
Core Subject : Health,
Health Professions
Jurnal Biotek Medisiana Indonesia published 2 times a year. This journal is a medium of information and research results and development areas for non-communicable diseases and public health program managers, as well as a means of communication the researchers /enthusiasts in the field of non-communicable diseases and infectious.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue " Vol 3, No 1 (2014)" : 5 Documents clear
Perencanaan Surveilans Brucellosis pada Manusia di Jawa Barat dengan Menggunakan Metode Geographical Information System (GIS) Novita, Risqa
Jurnal Biotek Medisiana Indonesia Vol 3, No 1 (2014)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.096 KB)

Abstract

Brucellosis is a zoonotic disease transmitted from animals and humans. The main source of brucellosis are ruminant animals such as dairy cows and sheep. Symptoms of human brucellosisis acute and non-specific that the reporting of cases are very rare. GIS-based surveillance is needed to detect Brucellosis disease patterns so that it can be made effective prevention programs, it aims to achieve optimal public health.The study was a reviewer from literature in Google and Pubmed by searching keywords are human Brucellosis, surveilance of human brucellosis and geographical information system (GIS). Based on the results of the literature searching, the use of GIS methods in human Brucellosis surveillance is still rare due to the low number of recording cases of Brucellosis in humans.Publics are also not know that brucellosis is zoonotic disease, furthermore clinical symptoms of brucellosis is acute and not specific.Surveillance of Brucellosis using GIS methods is necessary to ensure the prevention of brucellosis in animals and humans can be performed effectively in order to reach animal and human health systems are optimal.Key words : Human brucellosis, Surveillance, GIS AbstrakBrucellosis adalah penyakit yang ditularkan dari binatang ke manusia. Sumber penularan utama brucellosis adalah sapi perah dan domba. Gejala klinis brucellosis pada manusia bersifat akut dan tidak spesifik, sehingga catatan laporan kasus brucellosis di manusia sangat jarang. Surveilans yang berbasis Geographical Information System (GIS) diperlukan untuk mendeteksi brucellosis sehingga dapat menghasilkan program pencegahan yang efektif sehingga akan tercapai kesehatan masyarakat yang optimal. Kajian ini merupakan hasil dari telaah literatur hasilpenelusuran internet yang didapatkan dari Pubmed, CDC, FAO dan WHO melalui pencarian kata kunci yaitu brucellosis manusia dan GIS. Berdasarkan dari pencarian literatur, penggunaan GIS dalam surveilans manusia masih sangat jarang karena rendahnya angka pelaporan kasus brucellosis di manusia. Masyarakat belum banyak yang mengetahui potensi zoonosis dari brucellosis ditunjang gejala klinis yang ditimbulkan bersifat akut dan tidak spesifik. Surveilans brucellosis memakai metode GIS penting untuk menetapkan program pencegahan brucellosis di hewan dan manusia yang efektif agar tercapai sistem kesehatan manusia yang optimal.Kata Kunci : Brucellosis manusia, Surveilans, GIS
Manifestasi Klinis Infeksi Virus Chikungunya pada Kejadian Luar Biasa di Indonesia Maha, Masri Sembiring; -, Subangkit
Jurnal Biotek Medisiana Indonesia Vol 3, No 1 (2014)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.133 KB)

Abstract

Chikungunya is a re-emerging disease that caused byalphavirus family Togaviridae. Chikungunya also can often caused outbreak in Asia, including Indonesia. Disease characterized by fever accompanied by pain in the joints of the dominant. Chikungunya virus is transmitted by mosquitoes, especially Aedes aegypti, A. Albopictus and Mansoniasp. This study was conducted to identify significant clinical symptoms of chikungunya virus infection. Sampling was carried from Chikungunya outbreak area. Suspected Chikungunya are interviewed and taken blood. Specimens were examined using reverse transcription - polymerase chain reactions (RT-PCR). Interviews were conducted on111suspectedchikungunya from 6 provinces.Clinical symptoms analisys was performed from 28 confirm chikungunya cases based on laboratory result. Chikungunyashowedmore onthe malesex, and the productive age group (64.3%). The most joint pain symptom reported is wristand knee joints (92.9%), followed by the ankle joint (75%) and finger joints (64.3%). Othernon-specific clinical symptomssuch asheadaches(78.6%), decreased appetite(71.4%), nausea/vomiting(60.7%), feverwith chills(55%), and abdominal pain andvisual impairment(25 and17.9% ). Laboratory results showed 28 positive specimen for Chikungunya.Chikungunya is more common in males and productive age groups, with the joint pain symptoms especially knee and wrist joints followed by non -specific symptoms such as headache, decreased appetite and also nausea/vomiting.Key words : Chikungunya, Outbreak, Clinical symptoms, Indonesia. Abstrak Chikungunya disebabkan alphavirus famili Togaviridae, merupakan penyakit re-emerging yang sering menyebabkan kejadian luar biasa (KLB) di kawasan Asia termasuk Indonesia, ditandai dengan demam disertai nyeri pada sendi yang dominan. Virus chikungunya ditularkan oleh nyamuk terutama Aedes aegypti, A. albopictus dan Mansonia sp. Penelitian ini dilakukan bertujuan melihat gejala klinis pada infeksi virus chikungunya. Pengambilan sampel dilakukan di daerah yang melaporkan Kejadian Luar Biasa chikungunya. Suspek Chikungunya diwawancara dan diambil darahnya. Spesimen tersebut diperiksa menggunakan reverse transcription-polymerase chain reactions (RT-PCR).Wawancara dilakukan pada 111 suspek chikungunya yang tersebar pada 6 Propinsi. Analisis gejala klinis dilakukan pada 28 orang kasus terkonfirmasi dengan hasil laboratorium positif. Hasil menunjukkan penyakit chikungunya lebih banyak pada laki-laki, dan kelompok usia produktif (64.3 %). Keluhan nyeri sendi yang paling banyak dialami adalah keluhan pada sendi pergelangan tangan dan sendi lutut (92.9%), diikuti dengan sendi pergelangan kaki (75%) dan sendi jari tangan (64.3%).Gejala klinis non spesifik lain juga dikeluhkan seperti sakit kepala (78.6%), nafsu makan menurun (71.4%), mual/muntah (60.7%), demam disertai menggigil (55%), sakit perut dan dan gangguan penglihatan (25 dan 17.9%).Penyakit chikungunyalebih banyak menyerang laki-laki dan kelompok usia produktif, dengan keluhan yang utama adalahnyeri sendi, terutama sendi lutut dan pergelangan tangan dikuti dengan gejala non spesifik seperti sakit kepala, nafsu makan menurun dan mual/muntah.Kata Kunci: Chikungunya, Wabah, Gejala klinis, Indonesia
Deteksi Virus Rabies pada Kasus Ante-Mortem dengan RT-PCR -, Agustiningsih; Puspa, Kartika Dewi; Nugraha, Arie Ardiansyah; Setiawaty, Vivi
Jurnal Biotek Medisiana Indonesia Vol 3, No 1 (2014)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.897 KB)

Abstract

Rabies virus detection using Reverse Transcriptase Polymerase Chain Reaction (RT – PCR) was considered to have high sensitivity and specificity. This method is relatively faster and easy to perform in comparison with other methods such as Fluorescent Antibody Test (FAT) and Mouse Inoculation Test (MIT). Therefore, the RT PCR method is used as ante mortem diagnosis of rabies. A total of 74 specimens such as saliva, conjuctival swabs and under tongue swabs were collected from the bites of animal transmitting rabies cases. The 74 specimens were collected from 28 human cases of suspected rabies outbreaks in 2009-2013 that has been tested in the Virology Laboratory, Center for Biomedical and Basic Technology of Health. All specimens examined by RT - PCR method using two primer pairs that amplify the partial gene regions of the N and G genes of the rabies virus. Two of the 74 specimens gave positive results of rabies by RT - PCR, i.e saliva and under-tongue swab from human cases of animal bites in 2009. RT-PCR assay can be used for ante mortem diagnosis of the rabies virus. The laboratory results are influenced by the type of specimen and collection time.Key words : Rabies, Outbreak, RT-PCR AbstrakDeteksi virus rabies menggunakan metode Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) dinilai memiliki sensitivitas dan spesifisitas yang tinggi. Kemudahan pengerjaan dan hasil pemeriksaan yang relatif cepat dibandingkan dengan metode Fluorescent Antibody Test (FAT) dan Mouse Inoculation Test (MIT) yang merupakan metode standar WHO yang menjadikan RT-PCR lebih banyak digunakan untuk deteksi ante mortem virus rabies. Sebanyak 74 spesimen kasus gigitan hewan penular rabies (GHPR) yaitu saliva, apus selaput mata dan apus bawah lidah yang berasal dari 28 kasus tersangka rabies pada kejadian luar biasa pada tahun 2009-2013 yang telah diperiksa oleh laboratorium Virologi Pusat Biomedis dan Teknologi Dasar Kesehatan. Seluruh spesimen diperiksa dengan metode RT-PCR menggunakan 2 pasang primer yaitu primer yang mengamplifikasi sebagian region pada gen N dan gen G dari virus rabies tersebut. Dua dari 74 spesimen memberikan hasil positif rabies berdasarkan hasil pemeriksaan dengan RT-PCR, yaitu spesimen saliva dan swab bawah lidah dari kasus gigitan anjing pada tahun 2009. Pemeriksaan ante mortem terhadap virus rabies dapat menggunakan metode RT-PCR dengan memperhatikan waktu pengambilan dan jenis spesimen.Kata Kunci : Rabies, KLB, RT-PCR
Produksi Embryonic Stem Cell (Esc) Line dari Blastosis Mencit dengan Metode Immunosurgery Rinendyaputri, Ratih; Susanti, Nike
Jurnal Biotek Medisiana Indonesia Vol 3, No 1 (2014)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.563 KB)

Abstract

Embryonic Stem Cells (ESC) are pluripotent stem cells which has the ablility to self renew and differentiate into form all cells in the body. Inner Cell Mass (ICM) as a source of ESC that can be obtained from blastocyst stage of embryos. Isolation can be done by several methods such as mechanical, enzymatic and immunosurgery. This study aimed to observe the effectiveness of usage immunosurgery method to obtain the ICM from the blastocyst stage of embryo. Blastocyst stage of embryos were obtained from a strain of Swiss Webster female mice that had been stimulated using pregnant mare s gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Inner Cell Mass (ICM) were cultured and observed of the attachment level (attachment rate/AR), the rate of primary colony formation (primary colony/PC) and the morphology of ESC line. The results showed that the ICM were isolated using methods immunosurgery have AR and PC respectively 78,57% and 71,00%. Embryonic Stem Cell (ESC) can differentiate after several passages by forming embryoid body (EB). This study shows that immunosurgery is an effective method for producing ESC line from mice embryos of blatocyst stage.Key words: Blastocyst, Inner cell mass, ICM, ESC AbstrakEmbryonic Stem Cell (ESC) merupakan sumber stem cell yang bersifat pluripoten, yaitu sel yang mampu membelah dan berdiferensiasi menjadi semua tipe sel di dalam tubuh. Inner Cell Mass (ICM) sebagai sumber ESC dapat diperoleh dari embrio tahap blastosis. Isolasi ICM dapat dilakukan dengan beberapa metode seperti mekanik, enzimatik dan immunosurgery. Penelitian ini bertujuan mengamati efektifitas penggunanaan metode immunosurgery untuk memperoleh ICM dari embrio tahap blastosis. Embrio tahap blastosis diperoleh dari mencit betina strain Swiss Webster yang telah distimulasi menggunakan pregnant mare’s gonadotropin (PMSG) dan human chorionic gonadotropin (hCG). Inner Cell Mass (ICM) dikultur dan dilakukan pengamatan terhadap tingkat perlekatan ICM (attachment rate/AR) serta tingkat pembentukan koloni primer ESC (primary colony/PC) serta morfologi ESC line yang terbentuk. Hasil menunjukkan bahwa ICM yang diisolasi menggunakan metode immunosurgery memiliki AR dan PC masing-masing 78,57% dan 71,00%. Embryonic Stem Cell (ESC) dapat mengalami diferensiasi setelah beberapa pasase dengan membentuk embryoid body (EB). Penelitian ini menunjukkan bahwa metode immunosurgery merupakan metode yang efektif untuk memproduksi ESC line dari embrio mencit tahap blatosis.Kata kunci : Blastosis, Inner cell mass, ICM, ESC
Penerapan Diagnostik Laboratorium pada Kasus Tersangka Positif Difteri pada Kejadian Luar Biasa di Kota Pontianak, Kalimantan Barat Sariadji, Kambang; -, Sunarno; Putranto, Rudi Hendro
Jurnal Biotek Medisiana Indonesia Vol 3, No 1 (2014)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.351 KB)

Abstract

Based on national health research 2010, the coverage of basic immunization on West Kalimantan were 52,1%. It’s causes concern of outbreaks some immunizable prevented diseases in West Kalimantan . One of them is diphtheria. It was reported incidence of diphtheria suspect in Dalam Bugis, Pontianak Timur, Kota Pontianak, West Kalimantan in April 2013. A suspect of 12 years old girl was reported with a clinical diagnosis of diphtheria. Confirmation of positive suspect of diphtheria outbreaks occurred in West Kalimantan. A suspected case of diphtheriae and her mother were taken throat swabs on April 4, 2013, then she was retaken throat swabs and also 29 of her classmate as a contacts of case on April 24th 2013. Microscopic examination by staining Albert. Culture and isolation using blood agar and blood agar telurit. Suspect colonies are followed by biochemical tests using the API Coryne commercial product. Toxigenic test performed by polymerase chain reaction. A suspected case throat swab was taken on 4 April 2013 shows a microscopic results found difteroid form. Then the results of culture, isolation and biochemical have possibility > 89.5% of bacteria Corynebacterium diphtheriae type mitis. Toxigenic test showed that positif toxigenic bacteria which characterized by amplification gene tox (dtx) along 248 bp. Meanwhile her mother is a negative culture results C.diphtheriae. On 24th April 2013 the suspected case was re-taken of throat swab including 29 contacts from her classmate which showing negative culture result of C.diphtheriae. The suspected case was infected of C.diphtheriae type mitis toxigenic. It’s cases makes an outbreak in West Kalimantan.Key words : Corynebacterium diphtheriae, Culture and isolation, Biochemical test AbstrakRendahnya cakupan imunisasi dasar di Provinsi Kalimantan Barat menyebabkan kekhawatiran timbulnya penyakit yang dapat dicegah dengan imunisasi (PD3I) salah satunya adalah difteri. Kejadian difteri yang dilaporkan ini terjadi di Kelurahan Dalam Bugis, Kecamatan Pontianak Timur, Kota Pontianak, Provinsi Kalimantan Barat pada bulan April 2013. dilaporkan adanya kasus suspek dengan diagnosis klinis difteri pada anak perempuan usia 12 tahun. Mengidentifikasi suspek positif kasus kejadian luar biasa difteri yang terjadi di Kalimantan Barat. Suspek dan ibu suspek diambil swab tenggorok tanggal 4 April 2013, kemudian suspek diambil swab tenggorok lagi beserta 29 kontak teman sekolahnya pada tanggal 24 April 2013. dilakukan pemeriksaan secara mikroskopik dengan pewarnaan Albert. Kultur dan isolasi menggunakan medium agar darah dan telurit agar darah. Koloni tersangka dilanjutkan dengan uji biokimia menggunakan produk komersial API Coryne . Uji toksigenitas dilakukan dengan polymerase chain reaction Suspek yang diambil swab tenggorok pada tanggal 4 April 2013 menunjukkan hasil mikroskopis ditemukan bentuk difteroid. Hasil kultur, isolasi dan uji biokimia menunjukan possibility > 89,5 % bakteri Corynebacterium diphtheriae tipe mitis. Uji toksigenitas menunjukkan bakteri tersebut toksigenik yang ditandai dengan terbentuk produk amplifikasi dari gen dtx (tox) sepanjang 248 pb. Sementara hasil kultur Ibu suspek negatif C.diphtheriae. Pada pengambilan swab tenggorok tanggal 24 April 2013 terhadap suspek yang sama dan 29 kontak dari teman kontak menunjukkan hasil kultur negatif C.diphtheriae. Suspek terinfeksi C.diphtheriae tipe mitis toksigenik yang menjadikan Kasus tersebut menjadi kasus Kejadian Luar Biasa di provinsi Kalimantan Barat.Kata Kunci : Corynebacterium diphtheriae, Kultur dan isolasi, Uji biokimia

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2014 2014


Reset
Filter By Issues
All Issue Vol 5, No 2 (2016): : Vol 5, No 1 (2016) Vol 4, No 2 (2015) Vol 4, No 1 (2015) Vol 3, No 2 (2014) Vol 3, No 1 (2014) Vol 2, No 2 (2013) Vol 2, No 1 (2013) Vol 1, No 2 (2012) Vol 1, No 1 (2012) Vol 1, No 1 (2009) More Issue