cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Jurnal Biotek Medisiana Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 23015810     EISSN : 23548800     DOI : -
Core Subject : Health,
Health Professions
Jurnal Biotek Medisiana Indonesia published 2 times a year. This journal is a medium of information and research results and development areas for non-communicable diseases and public health program managers, as well as a means of communication the researchers /enthusiasts in the field of non-communicable diseases and infectious.
Arjuna Subject : -
Articles 64 Documents
 3   4   5   6   7 
Peran Sitoglobin dalam Mencegah Stres Oksidatif Werdhasari, Asri; Prijanti, Ani Retno; Jusman, Sri Widia
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5147.

Abstract

,
Status Antibodi Filariasis Penduduk Pasca Pemberian Obat Pencegah Massal (POPM) Secara Menyeluruh dan Parsial di Wilayah Endemis Filariasis Anorital, Anorital; Indiarto, Aris Hadi
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5149.

Abstract

,
Studi Kasus Bordetella Pertussis pada Kejadian Luar Biasa di Kabupaten Kapuas Kalimantan Tengah yang Dideteksi dengan PCR Sariadji, Kambang; Rizki, Aulia; Sunarno, Sunarno; Puspandari, Nelly; Rachmawati, Faika; Muna, Fauzul; Khariri, Khariri; Heriyanto, Bambang; Putranto, Rudi Hendro
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5151.

Abstract

,
Prevalensi dan Pola Resistensi N.gonorrhoeae Terhadap Beberapa Antibiotik pada Wanita Penjaja Seks di Jakarta Timur, Tangerang dan Palembang Tahun 2012 Puspandari, Nelly; Roselinda, Roselinda; Sunarno, Sunarno; Khariri, Khariri; Sariadji, Kambang; Yekti, Rabea Pangerti; Rifati, Lutfah; Pasaribu, Luxy Riajuni; Muna, Fauzul; Rohaeni, Rini; Kipuw, Natalie; Wati, Melati; Syamsidar, Syamsidar; Sofia, Sundari Nur; Amalia, Novi; Daily, Sjaiful Fahmi
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5152.

Abstract

,
Faktor-faktor yang Mempengaruhi Hasil Pemantapan Mutu Eksternal Pemeriksaan Glukosa, Kolesterol dan Trigliserida Laboratorium Klinik Mandiri di Indonesia tahun 2011 Suhardi, Suhardi; Hasugian, Armedy; Anorital, Anorital
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5153.

Abstract

,
Gambaran Mikroksopis, Molekular dan Klinis Kasus Malaria Hasil Survey Darah Masal di Kabupaten Katingan dan Kapuas, Provinsi Kalimantan Tengah Handayani, Sarwo; Dewi, Rita Marleta; Prasetyorini, Budi
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5154.

Abstract

,
HLA-DRB1 on Malaria Patients in Central Kalimantan : a Premilinary Study Handayani, Sarwo; Siswantoro, Hadjar; Triastuti, Tya; Rohmawati, Eny; Tjitra, Emiliana
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7697.85-93

Abstract

Human Leucocyte Antigen merupakan marker genetik yang membantu memfasilitasi presentasi antigen sehingga dikenali oleh sel limfosit T. HLA-DRB1 seperti HLA-DRB1*10:01 dan HLA-DRB1*13:02 merupakan HLA tipe 2 yang berkaitan dengan proteksi, sedangkan HLA-DRB1*04:01 berkaitan dengan kerentanan terhadap malaria berat. Oleh karena itu identifikasi alel HLADRB1 pada pasien malaria di Indonesia perlu dilakukan. Sampel berupa 40 spot darah kering yang berasal dari 7 penderita malaria berat dan 22 malaria tanpa komplikasi (11 P falciparum dan 11 P vivaks) yang diperoleh dari RS Doris Sylvanus Palangkaraya, serta 11 non malaria yang tinggal di daerah endemik malaria di Kalimantan Tengah. Setelah DNA sampel diekstraksi kemudian diperiksa tipe alel HLA-DRB1 dengan kit LABType® SSO DRB1 Typing Test (one Lambda). Intensitas floresens dari phycoerythrin selanjutnya diukur dengan alat Luminex dan data dianalisa dengan program  HLA fusion2.  Kisaran umur penderita adalah 3-90 tahun dengan proporsi laki-laki sebesar 62.5%. Hanya 87.5% sampel and 15 variasi alel HLA-DRB1 yang berhasil diidentifikasi. Dua alel yang paling dominan adalah HLA-DRB1*15:02 (23.8%) and HLA-DRB1*15:01(18.7%). Alel lain yaitu HLA-DRB1*01:01, HLA-DRB1*08:01 and HLA-DRB1*12:01 hanya ditemukan pada malaria berat, akan tetapi alel spesifik HLA-DRB1*04:01 yang berkaitan dengan kerentanan terhadap malaria berat tidak ditemukan. Dua dari empat alel homozigot yaitu HLA-DRB1*07:01 dan HLA-DRB1*15:01 tidak ditemukan pada malaria berat. Meskipun dengan sampel yang terbatas dan belum menggambarkan alel spesifik pada kelompok sampel, namun studi pendahuluan ini cukup bermanfaat untuk pemeriksaan dan anaIisis HLA lebih lanjut. Perlu sampel yang lebih banyak dan analisis yang lebih mendalam untuk mendapatkan data HLA yang lebih lengkap.
Studi Kajian Upaya Pemberian Obat Pencegah Masal Filariasis Terhadap Pengendalian Penyakit Infeksi Kecacingan Anorital, Anorital; Dewi, Rita Marleta; Retnoningtyas, Kristina Palupi
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7699.95-103

Abstract

Abstract Filariasis and helminth infections are public health problems in Indonesia. Starting in 2005, the Ministry of Health launched filariasis elimination by implementing preventive mass drug administration (MDA) or “POPM”. At least as  65% of the population in the district/city are given diethyl carbamazine citrate (DEC) and combined with albendazole. With the existence of this program, it is expected  filariasis can be eliminated and worm infections can be controled. This study aims to determine the constraints and problems encountered in the implementation of filariasis elimination and helminth infections control. The method applied is a review of scientific article, policy  inventory, discussions with experts and practitioners, as well as confirmation data in the field. This study known 233 districts/cities in Indonesia were endemic filariasis with mf rate average of 3.61%, and the prevalence of helminth disease in 173 districts/cities with an average of 28.12%. Among the 233 districts/cities endemic filariasis, 104 districts/cities were also endemic helminth diseases; and only 135 regencies/cities  implemented MDA filariasis. Within the 135 districts/cities reported to implement MDA filariasis, only 16 districts/cities actually executed MDA with prevalence of  helminth infections above 30% (MDA helminth requirement). Recommendations is necessary to accelerate MDA filariasis in the district/city with endemic filariasis and also those with endemic helminth diseases above 30%.
Pengaruh Fibronektin pada Kultur Sel Punca Limbal (SPL) Tikus Rinendyaputri, Ratih; Dany, Frans; rifati, Lutfah
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7700.113-120

Abstract

Abstract Availability of limbus stem cells (LSC) is very limited considering the number of donors SCL very less as compared to the needs, so it is necessary LSC production in vitro. The success of in vitro culture of LSC is strongly influenced by environmental conditions, one of which is the use of the extracellular matrix. Selection of the appropriate type of matrix in the LSC culture, can increase proliferation and facilitate the application of transplantation. This study aimed to get activities fibronectin (FN) as extracellular matrix to produce LSC. Research conducted at the Center for Biomedical and Basic Technology of Health. In this study, production of SPL rats performed in vitro using the method of explants on a petri dish with FN as the extracellular matrix. SPL proliferation rate that with and without FN analyzed by calculating the population doublings (PD), PD/day and the population doubling time (PDT). SPL characterization was performed by quantifiying RNA of CD90 gene, p63, ABCG2 and Krt12 as a marker SPL. The results showed that the PD,PD/day PDT in the SPL with and without FN respectively are 2.13 and 2.11, 0.44 and 0.42 , 57.65 and 61.46 (p> 0.05). While the RNA quntitative of CD90  , p63, ABCG2 and Krt12 SPL on with and without of FN respectively are 17.70 and 19.75, 17.08 and 18.42, 15.23 and 19.09, 17.42 and 18.85 (p> 0.05). This shows that the FN isn’t effective as an extracellular matrix in in vitro cultureSPL.Abstract       Availability of limbus stem cells (LSC) is very limited considering the number of donors SCL very less as compared to the needs, so it is necessary LSC production in vitro. The success of in vitro culture of LSC is strongly influenced by environmental conditions, one of which is the use of the extracellular matrix. Selection of the appropriate type of matrix in the LSC culture, can increase proliferation and facilitate the application of transplantation. This study aimed to get activities fibronectin (FN) as extracellular matrix to produce LSC. Research conducted at the Center for Biomedical and Basic Technology of Health. In this study, production of SPL rats performed in vitro using the method of explants on a petri dish with FN as the extracellular matrix. SPL proliferation rate that with and without FN analyzed by calculating the population doublings (PD), PD/day and the population doubling time (PDT). SPL characterization was performed by quantifiying RNA of CD90 gene, p63, ABCG2 and Krt12 as a marker SPL. The results showed that the PD,PD/day PDT in the SPL with and without FN respectively are 2.13 and 2.11, 0.44 and 0.42 , 57.65 and 61.46 (p> 0.05). While the RNA quntitative of CD90  , p63, ABCG2 and Krt12 SPL on with and without of FN respectively are 17.70 and 19.75, 17.08 and 18.42, 15.23 and 19.09, 17.42 and 18.85 (p> 0.05). This shows that the FN isn’t effective as an extracellular matrix in in vitro cultureSPL.
Ekspresi dan Purifikasi Protein Rekombinan Non-Struktural NS1 Virus Dengue Serotipe 1 Strain Indonesia Pada Pichia Pastoris Lestari, Chirstina Whinie; Narita, Vany; Soediro, Tjahjani Mirawati; Sjarurachman, Agus
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7702.121-132

Abstract

Dengue infection remains a public health problem in Indonesia. In the framework of the independence of the national product, pursued research and development of raw materials that can be used for diagnostic kit and vaccine candidates. The objective of this study was to express and purify recombinant proteins non-structural NS1 dengue virus serotype 1 (DENV-1) of Indonesia strain (915 846) in Pichia pastoris expression system with “halal” system that can be used for the development of diagnostic kits and vaccines. The dengue virus serotype 1 strain Indonesia (915846) was used to construct the NS1 DENV-1 gene by PCR. Then NS1 DENV-1 gene cloned into the vector pPICZαB and transformed into Pichia pastoris yeast cells, then verified with sequencing. Recombinant protein NS1 DENV-1 was secreted expression in P. pastoris. Characterization of antigenicity was did by ELISA, Western Blot and purified by the method of metal-chelating affinity chromatographic (MCAC) column. The recombinant protein with a size of 45 kDa secreted into the supernatant of P. pastoris with methanol induction and purified. The recombinant protein can bind to monoclonal antibodies and serum of patients infected with dengue virus serotype 1. These results indicate that the recombinant protein NS1 DENV-1 strain Indonesia (915 846) can be produced efficiently in P. pastoris secreted expression system is halal and still functional as an antigen that can be used for the development of diagnostic kits and vaccines.

Page 6 of 7 | Total Record : 64

 3   4   5   6   7 

Filter by Year

2009 2016


Reset
Filter By Issues
All Issue Vol 5, No 2 (2016): : Vol 5, No 1 (2016) Vol 4, No 2 (2015) Vol 4, No 1 (2015) Vol 3, No 2 (2014) Vol 3, No 1 (2014) Vol 2, No 2 (2013) Vol 2, No 1 (2013) Vol 1, No 2 (2012) Vol 1, No 1 (2012) Vol 1, No 1 (2009) More Issue