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INDONESIA
Jurnal Biotek Medisiana Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 23015810     EISSN : 23548800     DOI : -
Core Subject : Health,
Health Professions
Jurnal Biotek Medisiana Indonesia published 2 times a year. This journal is a medium of information and research results and development areas for non-communicable diseases and public health program managers, as well as a means of communication the researchers /enthusiasts in the field of non-communicable diseases and infectious.
Arjuna Subject : -
Articles 64 Documents
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Infeksi Human Parainfluenza Virus (HPIV) Pada Balita Dengan Infeksi Saluran Pernafasan Akut Berat di RSU Propinsi Nusa Tenggara Barat Tahun 2014 ekawati, hartanti dian; Puspa, Kartika Dewi; Setiawaty, Vivi; Susilarini, Ni Ketut
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7703.133-141

Abstract

Acute respiratory infection (ARI) is a major cause of morbidity and mortality worldwide, especially in children. An estimated 1.9 million children die because of ARI each year, with 70% of deaths occurred in Africa and Southeast Asia. Human Parainfluenza Virus (HPIV) is the second etiology of ARI in children after Respiratory Syncytial Virus (RSV). This study aims to determine the infection of HPIV in children under 5 years old with ARI at Nusa Tenggara Barat Province Hospital in 2014. Seventy five of 88 archived RNA from children under 5 years old with CT (RNP) number <27 were selected with proportional random sampling. The specimens were tested for HPIV with gel-based RT-PCR using spesific primer for HPIV 1, 2 and 3. Of 75 specimens tested, none is positive for HPIV-2 and only one specimen positive for HPIV-3. This specimen came from 1 year old female from Mataram District. Primer for HPIV-1 failed to optimized, therefore none of the sample is tested with HPIV-1. This finding proved that there was HPIV infection in children under 5 years old with severe cases of ARI at Tenggara Barat Province Hospital in 2014.
Perbandingan Pemeriksaan Toksigenisitas secara Genotip dan Fenotip pada Beberapa Isolat Corynebacterium diphtheriae Penyebab Difteri di Indonesia Sunarno, Sunarno; Sariadji, Kambang
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7704.143-151

Abstract

Corynebacterium diphteriae’s toxin can be detected by using in vivo and in vitro examination. Detection of the toxin using the guinea pig is a gold standard. This method requires a long time and has been opposed by animal right organization. There is another alternative method such as vero cell cytotoxicity and Elek test. The limitation of these methods are reagent procurements, technical skill, and a long processing time. Other alternative method like polymerase chain reaction (PCR) can overcome the limitation. PCR can detect the toxin quickly and the results can be interpreted easily. Some previous study showed there was the difference results between PCR and Elek test or vero cell cytotoxicity. The Aim of this study is to compare genotypic toxin detection using PCR and phenotypic detection using Elek test, on some isolates that caused diphtheria outbreak in Indonesia. A total of 12 isolates have been tested in this study. These isolates were obtained from the outbreak cases in Indonesia and isolated in Bacteriology Laboratory, Center for Research and Development of Biomedical and Basic Technology of Health. This study used reference strains as positive and negative controls (NCTC 10648, NCTC 3984 and NCTC 10356). All samples were examined by PCR for toxin genotyping detection, and Elek test for phenotyping detection. The results showed as many as 10 isolates were toxigenic C. diphtheriae while the remaining were non-toxigenic. There was no nontoxigenic tox-gene bearing (NTTB). There were no difference results between PCR and Elek test to detect the toxin.          Base on the results of this study, there was a 100% conformity results between PCR and Elek test to detect the toxin of C. diphteriae.
Hubungan Antara HbA1c Dengan LDL-K dan Albuminuria pada Penderita DM dengan Riwayat Komplikasi Jantung Koroner Driyah, Srilaning; Rachmawati, Banundari; Asti, Herniah
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7705.153-162

Abstract

The prevalence of Diabetes Mellitus (DM) globally is elevated continually. Poor blood glucose control to diabetes patients caused both macrovascular complications (ex.coronary heart disease/CHD) and  microvascular complications (ex.nephropathy). Blood glucose controlled is need to be done in every 3 months regularly using HbA1C as a diabetic parameter, regarding to the high risk mortality of the complications. Low density lipoprotein cholesterol (LDL-C) is dyslipidemia  characteristics and LDL-C is a lipoprotein proatherogenic. Albuminuria test is also needed as an early marker of micro and macrovascular disorders, which reflects the general process of endothelial damages (vascular dysfunction).The aim of this study is to prove any relationship between HbA1C levels with LDL-C and albuminuria levels in diabetic patients with history of coronary heart complications.This study is performed an analytical observational study with cross sectional approach. Thirty diabetic patients with any history of CHD complications were enrolled, then laboratory analysis of HbA1C by ion exchange HPLC, LDL-C levels were using colorimetric enzymatic method and albuminuria test with  the photometric method. Differences between variables were analyzed using by Spearman correlation-testHbA1C levels in 10 patients (33,3 %) are <7% and in 19 patients are ≥ 7% (66,7 % ). LDL-C (<100mg/dl) in 13 patients (43,3%), LDL-C (≥100mg/dl) in 17 patients (56,7%), Normoalbuminuria (< 20 mg/L ) in 11 patients (36.7%), albuminuria (≥20 mg/L) in 19 patients (63,3%).There is a moderate positive relationship between HbA1C and LDL-C levels (r=0.385, p=0.014) and a positive strong relationship between HbA1C and albuminuria levels (r=0.52, p=0.004).Conclusions: The higher HbA1C levels, the higher LDL-C and albuminuria levels would be.
Profil Candida penyebab kandidemia dan pola kepekaan terhadap anti jamur pada pasien sakit kritis di Rumah Sakit Cipto Mangunkusuno Mursinah, Mursinah; Ibrahim, Fera; Wahid, Mardiastuti
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7706.105-111

Abstract

Candida spesies is important cause of nosocomial infection that lead to death and prolonged hospital stay. Guideline management of candidiasis by Infectious Diseases Society of America (IDSA) in 2009 proposed early antifungal therapy in critical patient with the risk of invasive candidiasis. Early identification of  sepsis patien with high risk of fungal infection is challenging because  long and low result from blood culture. Antifungal usage without appropriate indication can cause resistance to antifungal. The aim of the study is obtain data candida species that caused candidemia in Cipto Mangunkusuno hospital (RSCM) and sensitivity pattern to antifungal. This retrospective study used patient medical record from 2011-2014. This study had 117 candidemia case in RSCM during 2011-2014. Every year candidemia was dominated by Candida  tropicalis, Candida  albicans, and Candida  parapsilosis with trend the increase number of Candida  tropicalis since 2013. Susceptibility pattern of Candida that cause candidemia in RSCM from 71 isolat tested the result was86% spesies Candida flukonazol sensitive, 99% vorikonazol sensitive, 97% amfoterisin B sensitive and 100% flusitosin sensitive.

Page 7 of 7 | Total Record : 64

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