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Agrovigor: Jurnal Agroekoteknologi
Published by Universitas Trunojoyo Madura
ISSN : 1979577     EISSN : 24770353     DOI : https://doi.org/10.21107/agrovigor
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Agrovigor: Jurnal Agroekoteknologi is a scientific paper in the field of science Agroecotechnology which include: plant science, soil science, plant breeding, pest and plant diseases.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 329 Documents
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INDUKSI KALUS DAN DETEKSI KANDUNGAN ALKALOID DAUN JARAK (Jatropha curcas L.) MENGGUNAKAN HORMON 2,4-D DALAM MEDIA MS (MURASHIGE SKOOG) Yudi Rinanto
Agrovigor Vol 4, No 1 (2011): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v4i1.270

Abstract

The research was conducted to examine 2,4-D hormone on callus induction and the synthesis of alkaloids in Jarak (Jatropha curcas  L.) leaves callus on MS medium. The young leaves of Jarak are used as explant. The amount of 2,4-D hormone to be treated was 1 mg/l, 2  mg/l,  dan  3  mg/l, respectively.Evaluation of callus formation was done after 8 weeks incubation time. Qualitative analysis on the Alkaloid was done by color reaction and by TLC (Tin Layer Chromatoghraphy) with stationary phase silica gel GF 254 and asetat:metanol:air  (100:13,5:10) as movement phase. The chromatogram was observed under UV254 nm and UV366 nm lampsThe results showed 3 ppm concentration of 2,4-D hormone resulted highest growth of  callus formation (85%), the fastest time of callus induction (12.2 days) and average weight (0.054 grams) of dried callus. SimakBaca secara fonetikThe qualitative  analysis  with  Dragendorff  reagent  produced  positive  result  brown color in TLC  at UV254 nm, and  blue  color  in UV366 nm. hRf value was almost the same as wild type. According to the research, alkaloid also found in the callus as in the wild type plant.
OPTIMALISASI PEMANFAATAN LAHAN RAWA PASANG SURUT MELALUI PENGELOLAAN LAHAN DAN KOMODITAS Dakhyar Nazemi; A. Hairani; - Nurita
Agrovigor Vol 5, No 1 (2012): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v5i1.308

Abstract

Teknologi handal yang telah didapatkan dan diterapkan di lahan pasang surut , serta varietas yang adaptif telah terbukti mampu memperbaiki kualitas  dan meningkatkan produktivitas lahan pasang surut.  Pemanfaatan lahan pasang surut dapat ditingkatkan melalui penerapan teknologi penataan lahan sistem surjan dan pemilihan komoditas yang adaptif,  serta  pengelolaan air dengan sistem satu arah pada tipe luapan air  A dan B,  serta  sistem ‘tabat' (konservasi) pada tipe luapan air  C.  Untuk memperbaiki kondisi fisiko-kimia tanah maka bahan ameliorasi dan pupuk juga merupakan salah satu faktor penting. Untuk meningkatkan pendapatan dan mengurangi resiko kegagalan panen penganekaragaman komoditas perlu dilakukan .
ISOLASI cDNA SUCROSE TRANSPORTER (SUT) DARI BATANG TANAMAN TEBU (Saccharum officinarum L.) - Slameto; Bambang Sugiharto
Agrovigor Vol 3, No 2 (2010): SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v3i2.260

Abstract

Sucrose Transporter (SUT) is kind of protein transporter that control in sucrose translocation. Sucrose Transporter is intermediate in translocation of sucrose from apoplasmic to simplasmic. SUT facilitates sucrose transportation from vascular tissues to parenchyma cells toward in node sugarcane stem. This research was purposed to isolate cDNA SUT from sugarcane stem, and cloned in Escherichia coli strain DH5α. Total RNA of sugarcane stem was isolated by single step method, then add with oligo dT in order to obtain the first strand of SUT cDNA then used as template for PCR. The primer used for PCR is 5’ –ggg ctg att gtg gcc atg tc- ‘3 (SUT-F) and 5’ –tgc cct ttg tct ccg gaa cc- ‘3 (SUT-R). PCR was programmed as follow denaturation at 94°C for 2 minutes and 30 second, annealing at 54°C for 30 s, extension at 72°C 2 min and 7 min, and storage at 4°C for unlimited, It was for 30 cycles. Complementary DNA SUT from PCR ligalized to pTOPO bunt-end, then it cloned in to E. coli strain DH5α. The cloning resulted then be sequenced in order to observe the homologues with other nucleotides sequences of some plant using BLASTn program in GENE BANK NCBI and the level of homology determined by Genetyx program. The concentrated of total RNA isolated was 5,024 μg/μl, with purity of 1,85. Complementary DNA SUT fragment from PCR with size 2037 bp appropriated to the both of primer was used. Complementary DNA SUT fragment showed by analyzed some of restriction enzyme e.g. EcoRI, PstI and BamHI. Homologues of this cDNA SUT fragment was 100% to SoSUT 2A of sugarcane stem and 84% to OsSUT of rice plant (Casu et al ., 2003).
KARAKTER FISIOLOGIS KLON KOPI ROBUSTA BP 358 PADA JENIS PENAUNG YANG BERBEDA Ummi Sholikhah; Dena Munandar; Andri Pradana
Agrovigor Vol 8, No 1 (2015): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v8i1.749

Abstract

Shade plants for coffee plantation generally use dadap, lamtoro andothers. Today there many change the use of shade plant from lamtoro to sengon because economic value and increasing demand for sengon wood. Due to thechanging in different type of shade plant cause affect on coffee plantationmicroclimate. On the coffee plantation also use some coffee clones. The differences type of shade and coffee clones can affect the differences physiological and morphological characters of coffee plant that affect the production.The aim of this research is expected to give information about physiological characters of robusta coffee clones in different shade plant, factors that affect the robusta coffee photosynthesis process, the relationship of photosynthesis with the production and robusta coffee clones that have high production. This research was conducted in coffee plantation at Sidomulyo village, the district of Silo, Jember regency located at a 560 meters above sea level. This research was done on May up to June 2011. The area determination method was chosen based on the consideration that Sidomulyo village is one of the popular coffee producer in Jember. The experiment used field experiment with the quadrant method use to observation and collect the data of 12 years old BP 358. The support parameter observed were light intensity, temperature, humidity and assessed fruit production. The data from the observation wereproduction branch than photosynthesis activity. The result showed coffee clones BP 358  with sengon shade had higher photosynthesis activity than lamtoro shade. The photosynthesis activity was more affected by the stomatal conductivity and the light intensity. Keyword: Physiological Characters, Coffee Clones BP 358,  Types of Shade
PENGUKURAN KANDUNGAN SKOPOLETIN PADA BEBERAPA TINGKAT KEMATANGAN BUAH MENGKUDU (Morinda citrifolia Linn) DENGAN METODE KLT DENSITOMETRI Diana Nurus Sholehah
Agrovigor Vol 3, No 1 (2010): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v3i1.251

Abstract

Morinda citrifolia fruit has been used as remedies in Indonesia because many diseases could be healed. Scopoletin, one of major component of Morinda citrifolia was chosen as  marker compound because of  its various activites such as  hypotensive, antiinflamatory and antihistamine. The research aimed to determinate scopoletin in some maturations stage of Morinda citrifolia fruit in order to get best fruit for traditional medicine and phytopharmaca with optimal therapeutical effect . A simple and reliable TLC-Densitometry which has been validated before, was choosen to this aim. Fruit at stage 1,2,3 and 4 were taken  20, 45, 105 and 120 days after flowering. TLC was held on silica gel plates as stationary and ether : toluene : acetic acid 10 % (58:45:0,4) as mobile phase of fruit at stage 1, 2 and 4 and also ether : toluene : acetic acid 10 % (58:45:0,8) as mobile phase of fruit at stage 3.  Mean concentration of scopoletin at stage 1 was found to be (10,72±0,45) ppm, stage 2 was found to be (19,19±0,68) ppm, stage 3 found to be (57,94±0,79) ppm and stage 4 (14,11±0,39) ppm respectively.
HUBUNGAN SIFAT TANAH MADURA DENGAN KANDUNGAN MINYAK ATSIRI DAN TINGKAT KELARUTANNYA PADA JAHE (Zingiber offocinale L.) Sinar Suryawati; Eko Murniyanto
Agrovigor Vol 4, No 2 (2011): SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v4i2.299

Abstract

Pemanfaatan tanaman obat, seperti Jahe untuk berbagai kepentingan medis (biopropeksi) telah banyak diteliti, namun mempelajari ekologi edapic hubungannya dengan biosintesis senyawa utama seperti minyak atsiri belum banyak diungkap. Jika fenomena tersebut dapat diungkapkan maka memperbaiki kondisi edapic untuk kepentingan produksi dapat dirancang, setidaknya dapat dibuat zonasi potensi alamiahnya.Penelitian ini bertujuan untuk mempelajari hubungan antara sifat kimia tanah Madura dengan kandungan minyak atsiri dan tingkat kelarutan pada rimpang Jahe. Hasil penelitian di tiga wilayah kabupaten di Madura diperoleh kenyataan bahwa terjadi hubungan yang bervariasi pada setiap komponen kimia tanah dengan kandungan minyak atsiri rimpang jahe dan kelarutannya. Secara umum kandungan minyak atsiri rimpang jahe berkisar antara 1,07-1,09% pada semua komponen sifat kimia tanah Madura.
UJI KETAHANAN BEBERAPA GALUR KAPAS (Gossypium hirsutum ) HASIL RADIASI TERHADAP SERANGGA HAMA PENGGEREK BUAH Helicoverpa armigera(Hǖbner) Dwi Sunarto; - Nurindah; Lilik Harsanti
Agrovigor Vol 8, No 1 (2015): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v8i1.745

Abstract

Uji  ketahanan beberapa galur kapas (Gossypium hirsutum) terhadap  penggerek buah Helicoverpa armigera (Hǖbner) dilaksanakan di laboratorium Entomologi Balai Penelitian Tanaman Pemanis dan Serat Malang pada bulan Januari sampai dengan Mei 2011. Penelitian bertujuan untuk mengevaluasi ketahanan beberapa galur kapas hasil radiasi terhadap penggerek buah H. armigera.  Perlakuan disusun menggunakan Rancangan Acak Kelompok (RAK) yang terdiri atas empat galur hasil radiasi yaitu galur IA, 2A, 4A, dan 2C, dua varietas hasil radiasi yaitu Karisma, NIAB, dan dua varietas hasil pemuliaan konvensional yaitu Kanesia 10 dan Kanesia 15.  Setiap perlakuan diulang 3 kali.  Pengujian dilakukan dengan cara uji pakan (feeding assay) daun, kuncup daun, dan buah muda sesuai dengan perkembangan larva H. armigera.  Larva instar I, instar II-III, dan instar IV-V berturut-turut diberikan daun muda, kuncup bunga, dan buah muda. Hasil penelitian menunjukkan bahwa galur kapas nomor 1A, 2A, 4A, dan 4C merupakan galur yang toleran terhadap H. armigera.  Kata kunci : Gossypium hirsutum, Helicoverpa armigera,   ketahanan  varietas.
Trichogramma chilotraeae PARASITOID TELUR Helicoverpa armigera (Hubner) PADA POPULASI INANG RENDAH - Sujak; - Nurindah
Agrovigor Vol 4, No 1 (2011): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v4i1.275

Abstract

Helicoverpa armigera Hubner is an insect pest of corn and cotton crops. Eggs are laid by  H. armigera imago on corn silk and cotton crops often have high mortality, mainly caused by egg parasitoid. H. armigera egg on various agroecosystem reported can  be parasited by at least 12 species of  Trichogrammatidae. The purpose of this study was to determine the diversity of  Trichogrammatidae family as  parasitoid eggs of  H. armigera on  low population. H. armigera egg sample taken from Asembagus, Lamongan and Blora at 1 m2 field  both monoculture and intercropping. Observation of parasitoids and predators  done at the Laboratory of Biological Control Balittas Malang. Parasitoid that appears preserved in the Hoyer medium for identification purposes. low population of  H. armigera Egg in Asembagus is  4 eggs/m2 in both monoculture maize and intercropping with 86-100 days after transplanting (DAT) cotton and  4.5 egg/m2 on cotton monoculture and intercropping with 75  DAT soybean, while 2 eggs/m2 in the Lamongan and Blora corn agroecosystem. The dominant egg parasitoid in Asembagus is T. chilotraea, as well as in Lamongan and Blora.
PENGARUH PENGGUNAAN PUPUK UREA DAN APLIKASI HERBISIDA PRA-TUMBUH TERHADAP PERTUMBUHAN BIBIT KARET (Hevea brasiliensis Muell.Arg.) DAN GULMA DI PEMBIBITAN Yakup Parto; Yernelis Syawal; Teguh Achadi
Agrovigor Vol 5, No 2 (2012): SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v5i2.325

Abstract

A Research was carried out at green house of Agriculture Faculty of Sriwijaya University from October 2010 to January 2011 using split-split plot design. Main plot were pre-emergence herbicides :  H0 = no preemergence herbicide treatment (control), H1 = preemergence herbicide of methyl metsulfuron, and H2 = preemergence herbicide of ametryn. Subplot were dose of urea fertilizers : U0 = no urea fertilizer treatment (control), U1 = 2 gram of urea fertilizer per plant, and U2 = 4 gram of urea fertilizer per plant. Sub-sub plot were preemergence dosages : D0 = 0 liter formulation per ha, D1 = 1,5 liter formulation per ha, and D2 = 3,0 liter formulation per ha. Parameters measured were diameter of sleep eye stum, time of bud emergence, height of bud, number of leaves, percentage of growth seedling, weed growth, and efficiency of weed control. Results showed that kind of preemergence herbicides, urea fertilizer dosages, and preemergence herbicide dosages were no significant effect to weed dry weight due to weed growing were relative small. This supposed because soil which used as growth media no enough contain weed propagule, so the following research are need conducted.
PENGARUH MEDIA TANAM DAN NUTRISI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN PAKCHOI (Brassica juncea L.) DENGAN SISTEM HIDROPONIK Balia Perwitasari; Mustika Tripatmasari; Catur Wasonowati
Agrovigor Vol 5, No 1 (2012): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v5i1.304

Abstract

Pakchoi (Brassica juncea L.). is one kind of a vegetable crop that has high economic value and high nutrition. The technology of hydroponic is one of alternative cultivation using other media than soil substrates and nutrients. The purpose of this research is to determine the effect of media composition and nutrition on growth and yield of  pakchoi crops and find out which treatment is best for plant growth and yield of pakchoi. Researchcarried outinthe gardenexperiment ina plastichouse, Faculty ofAgriculture, University ofTrunojoyoMadura, at an altitude of±5 mabove sea level, temperature29ºC, and± 75% RH. The research was conductedin December2011 toFebruary2012.The analysis was usednonfaktorialRAL. Treatmentwithcomposemediatypes(raw rice husk, rice huskcharcoal, sand) andnutrients(no nutrients, premiumnutrition, nutritiongoodplant). There are ninetreatment, threereplicationsandthreesamples ofthe plant, furthertestswere analyzed byDMRT5%. Results showedthe treatmentcompositionandnutrientmediaprovidesignificantly differentresultsatdifferent ages ofobservationson eachvariableobservation. The besttreatmentcompositioncontained in thehusk charcoalandnutrientmediagoodplant(M2N2). Evidenced by theaverage ofthe highestresultson the length ofthe plant(29.38cm), number of leaves(22.22 strands), leaf area(3226.79 cm2), wetweight(242.19 g) and dry weight(13, 27g) totalplantpakchoiat age4 MST.

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