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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 352 Documents
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PRIMARY AND SECONDARY SOMATIC EMBRYOGENESIS OF CACAO: THE EFFECT OF EXPLANT TYPES AND PLANT GROWTH REGULATORS Nur Ajijah; Rr Sri Hartati
Indonesian Journal of Agricultural Science Vol 20, No 2 (2019): December 2019
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v20n2.2019.p69-76

Abstract

The success of cacao somatic embryogenesis is affected by many factors, including the basal salt medium, the genotype, the explant type, and the concentration and composition of plant growth regulators (PGRs). The study aimed to evaluate the effects of PGRs composition on the primary somatic embryo (PSE) response and the effect of explant type and PGRs composition used in inducing PSE on the secondary somatic embryogenesis (SSE) response. PSEs were induced from basal petal and staminoid explants of MCC 01 and MCC 02 clones on DKW medium containing 2,4-D 2 mg l-1 + kinetin 0.5 mg l-1 or 2,4-D 2 mg l-1 + kinetin 0.125 – 0.250 mg l-1 + thidiazuron (TDZ) 2.5 – 5 µg l-1 or 2,4-D 2 mg l-1 + TDZ 10 µg l-1. Genotype, explant type, and PGR composition dependently affected PSE response. The best PSE response was obtained from staminoid explant of MCC 02 clone on medium containing 2,4-D 2 mg l-1 + kinetin 0.5 mg l-1 (20%, 9 embryos). The explant type and PGR composition used in inducing PSEs affect the SSE response. The highest  SSE response of MCC 01 clone was obtained from petal explant with medium containing 2,4-D 2 mg l-1 + kinetin 0.5 mg l-1. The formation of SSEs could increase the multiplication rate of MCC 01 clone by 7 times.
IDENTIFICATION OF IRON TOLERANT CANDIDATE LOCI IN RICE DETERMINED THROUGH GENOME-WIDE ASSOCIATION STUDY Dwinita Wikan Utami, M.Si.; Ida Rosdianti; Lili Chrisnawati; Subardi Subardi; Siti Nurani; Suwarno Suwarno
Indonesian Journal of Agricultural Science Vol 21, No 1 (2020): June 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n1.2020.p17-29

Abstract

Iron (Fe) toxicity is a significant abiotic stress in swamp land. The study aimed to identify the candidate loci related to Fe toxicity tolerance through Genome-Wide Association Study (GWAS) approach.  The study used 288 rice accessions consisting of 192 breeding lines and 50 local landraces, and custom-designed 384 rice SNPs-chips. A field evaluation was conducted in inland swamp for two season periods (2014 and 2015). Phenotypic data and association mapping were analyzed using XLSTAT and TASSEL 3.0. The candidate loci were analyzed by functional gene detection of the significant SNPs aligned to the Rice Annotation Project and the Institute for Genomic Research databases. Three linkage disequilibrium (LD) blocks were detected in the Fe tolerant population around the significant SNPs. The first LD block was mapped in chromosome 1 (the AtIRT gene and qFETOX1; qFETOX1-3 QTLs loci) resembled partitioning of Fe-toxicity tolerant mechanism. The second LD blocks located in chromosome 2 (qFE-TOX-2-1 and qFETOX-2 QTLs loci) and chromosome 3 (qFETOX-3 QTL, OsNAS1 and OsNAS2 loci), probably contributed to Fe exclusion mechanism. The third LD blocks located in chromosome 4 (OsFRO2 and qFETOX-4 QTL loci) and chromosome 7 (OsIRT2 and NAS3 loci). The third LD block found on tolerant genotypes both on vegetative and generative stages. This condition indicated that these loci were presumed playing a role for Fe toxicity tolerance in rice. Result of the study are beneficial for determining the strategy on developing Fe-toxicity tolerant rice for specific swamp land type through breeding programs.
EFFECTS OF CRUDE SUGARCANE FACTORY EFFLUENT ON THE MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS OF CHICKPEAS Mohd. Suhail; K.P. Singh; Imran Ali
Indonesian Journal of Agricultural Science Vol 21, No 1 (2020): June 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n1.2020.p30-38

Abstract

Waste released from sugar factories has a huge amount of inorganic and organic matter whose effect on plant is different when used in different concentrations. Hence, the effects of sugar factory effluent on the morphological and biochemical parameters of chickpeas (Cicer arietinum Linn.) were studied by using the different concentrations of effluent for irrigation of chickpeas plant. The experiment was conducted at Botanical Garden, Department of Biochemistry, Bhaila (PG) College, Bhaila (Saharanpur), Uttar Pradesh. In the experiment, pots were arranged in a completely randomized design, replicated by six times and were labeled for the various treatments viz. 0%, 10%, 25%, 50%, 75% and 100% v/v. After that, chickpea plants were grown up to 10 days, in the soil irrigated with different concentrations of sugar factory effluent. It was observed that sugar factory effluent promoted the growth of chickpea seeds at 10–25% concentration, but its lethal impacts were noted when concentrations were above 50–100%. Hence, it was found that at lower concentrations (upto 25%), sugar factory effluent behaves as compost for better growth of chickpeas, but at high concentrations, it shows toxicity and behaves as a pollutant. Moreover, it makes the speed of biochemical reactions slow in chickpeas. Therefore, sugar factory effluent can be used for irrigation of chickpeas after proper dilution.
VARIABILITY AND HERITABILITY ESTIMATE OF 30 RICE LANDRACES OF LAMJUNG AND TANAHUN DISTRICTS, NEPAL Anup Dhakal; Shishir Sharma; Amrit Pokhrel; Ankur Poudel
Indonesian Journal of Agricultural Science Vol 21, No 1 (2020): June 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n1.2020.p1-10

Abstract

The variability in the rice landraces is the source of variation and offers substantial opportunity for the development of new varieties through the selection. Exploring diversity and variability of landraces is utmost for rice improvement. The objective of the study was to assess diversity indices, magnitude of genetic variability, heritability, genetic advance for the yield, and yield contributing traits. An experiment was carried out in alpha-lattice with 30 landraces, two replications in the Agronomy farm of IAAS, Lamjung Campus, during June-November, 2018. Shannon and Simpson's indices were calculated for 18 qualitative traits. The value of the Shannon and Simpson index ranged 0.15-1.41 and 0.07- 0.75, respectively. Landraces showed significant variations (p
IMPROVE YIELD AND DOWNY MILDEW RESISTANCE OF F1 MADURA MAIZE HYBRIDS Achmad Amzeri; Kaswan Badami
Indonesian Journal of Agricultural Science Vol 21, No 1 (2020): June 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n1.2020.p11-16

Abstract

Maize production in Madura Island is low. The study aimed to evaluate the agronomic performances (flowering age, harvesting age, and yield), heterosis effect, and resistance to downy mildew disease of F1 Madura maize hybrids. Ten F1 maize hybrids and seven respective parents were evaluated at the experimental center of the Agro-Technology Study Program of Agriculture Faculty, the University of Trunojoyo Madura, from July to December 2017. The experiments were arranged in a randomized block design, three replications, with a 50–100 plant population per unit. The parents were T12, T16, G10, G14, E02, Td04 and L1 genotypes having resistance to downy mildew (Peronosclerospora maydis). The results showed that the flowering age of the F1 Madura maize hybrids ranged 35–39 days, the harvesting age was 74–81 days, and the yield ranged from 2.90–6.40 t ha-1. Three hybrids showed the highest yield (T12 x L1 = 6.40 t ha-1, T16 x L1 = 5.42 t ha-1, and E02 x L1 = 5.90 t ha-1), and resistance to downy mildew, i.e. T12 x L1 (26.67%), T16 x L1 (26.67%), and E02 x L1 (26.67%). Two hybrids showed the highest heterosis values for yield, i.e. T12 x L1 (65.80%) and E02 x L1 (54.65%). The study suggests that three F1 Madura maize hybrids (T12 x L1, T16 x L1 and E02 x L1) are prospective to be developed further for high yield and resistance to downy mildew.
NUTRIENT CONTENT OF SOYBEAN MEAL FROM DIFFERENT ORIGINS BASED ON NEAR INFRARED REFLECTANCE SPECTROSCOPY Budi Tangendjaja
Indonesian Journal of Agricultural Science Vol 21, No 1 (2020): June 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n1.2020.p39-47

Abstract

Near infrared reflectance spectroscopy (NIRS) has become common techniques to estimate chemical composition of feed ingredient for poultry. Two experiments were performed: first was to compare the capability of NIRS system from three laboratories (E, A and T) to measure nutrient composition of soybean meal (SBM); and the second was to evaluate nutrient composition and quality of 59 samples of SBM from Argentine, Brazil and US using NIRS from T-laboratory. Thirty samples of SBM was used in the first study and the result showed that all NIRS systems were able to estimate proximate, amino acids, metabolizable energy (ME) and carbohydrate components. The second experiment indicated that there were some differences in proximate composition (especially protein), total amino acids and digestible amino acids among SBM from different origins. Brazilian SBM had 2% higher protein and amino acid compared to US or Argentine SBM (P<0.05). However, US SBM had slightly higher ME (20 and 40 kcal kg-1) compared to Brazilian and Argentine SBM, respectively. ME is positively correlated with protein (0.50) and fat content (0.58) but negatively correlated with fiber (-0.74) and NSP (-0.61). Stepwise regression analysis demonstrated that ME can be estimated using equation ME (kcal kg-1) = 75.7 – 21.0 x Fiber + 87.4 x Fat + 32.9 x Protein + 17.6 x NFE with reasonable accuracy (R2 = 0.995). In conclusion NIRS can be used to estimate nutrient content of SBM. Brazilian SBM has higher protein and amino acids, but US SBM has slightly higher ME content.
NUTRIENT CONTENT OF SOYBEAN MEAL FROM DIFFERENT ORIGINS BASED ON NEAR INFRARED REFLECTANCE SPECTROSCOPY Tangendjaja, Budi
Indonesian Journal of Agricultural Science Vol 21, No 1 (2020): June 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n1.2020.p39-47

Abstract

Near infrared reflectance spectroscopy (NIRS) has become common techniques to estimate chemical composition of feed ingredient for poultry. Two experiments were performed: first was to compare the capability of NIRS system from three laboratories (E, A and T) to measure nutrient composition of soybean meal (SBM); and the second was to evaluate nutrient composition and quality of 59 samples of SBM from Argentine, Brazil and US using NIRS from T-laboratory. Thirty samples of SBM was used in the first study and the result showed that all NIRS systems were able to estimate proximate, amino acids, metabolizable energy (ME) and carbohydrate components. The second experiment indicated that there were some differences in proximate composition (especially protein), total amino acids and digestible amino acids among SBM from different origins. Brazilian SBM had 2% higher protein and amino acid compared to US or Argentine SBM (P<0.05). However, US SBM had slightly higher ME (20 and 40 kcal kg-1) compared to Brazilian and Argentine SBM, respectively. ME is positively correlated with protein (0.50) and fat content (0.58) but negatively correlated with fiber (-0.74) and NSP (-0.61). Stepwise regression analysis demonstrated that ME can be estimated using equation ME (kcal kg-1) = 75.7 – 21.0 x Fiber + 87.4 x Fat + 32.9 x Protein + 17.6 x NFE with reasonable accuracy (R2 = 0.995). In conclusion NIRS can be used to estimate nutrient content of SBM. Brazilian SBM has higher protein and amino acids, but US SBM has slightly higher ME content.
USING GLOBAL CLIMATE INDICES TO PREDICT RAINFALL AND SUGARCANE PRODUCTIVITY IN DRYLANDS OF BANYUWANGI, EAST JAVA, INDONESIA Muhammad Rasyid Ridla Ranomahera; Bayu Dwi Apri Nugroho; Prima Diarini Riajaya; Rivandi Pranandita Putra
Indonesian Journal of Agricultural Science Vol 21, No 2 (2020): DECEMBER 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n2.2020.p78-88

Abstract

In Indonesia, sugarcane (Saccharum officinarum L.) is mostly cultivated in drylands, thus depending on rainfall for crop growth and development. Rainfall is an essential factor affecting sugarcane productivity. The global climate indices can be used to investigate potential of rainfall within a given area and its relationship with crop productivity. This reserach aimed to analyze the relationship between the global climate index, rainfall, and sugarcane productivity in drylands near Glenmore sugar mill, i.e., Benculuk and Jolondoro, Banyuwangi, East Java, Indonesia. The global climate index data used were the Southern Oscillation Index (SOI) and Sea Surface Temperature (SST) between 1995 and 2014. Results of this research showed that SOI and SST can be used to predict the rainfall in both Benculuk and Jolondoro. Rainfall (y) can be predicted with SST data (x) using the equation of y = -352.49x + 7724.1 in Benculuk and y = -107.32 + 3443.4 in Jolondoro, as well as with SOI data (x) using the equation of y = 38.664x + 1555.1 in Benculuk and y = 10.541x + 1567.8 in Jolondoro. Sugarcane productivity (y) in Jolondoro can be predicted using data of total rainfall (x) between October and March with the following equation: y = -0.1672x + 1157.3. This equation can be used by sugar mills, sugarcane growers, and other sugarcane-relevant stakeholders for determining the appropriate growing season.
EFFECT OF SCREEN HOUSE MODIFICATION AT FLOWER BUD STADIA ON FLOWER QUALITY OF CHRYSANTHEMUM AT MEDIUM UPLAND ELEVATION Herni Shintiavira; Endang Sulistyaningsih; Aziz Purwantoro; Rani Agustina Wulandari
Indonesian Journal of Agricultural Science Vol 22, No 1 (2021): JUNE 2021
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v22n1.2021.p27-38

Abstract

Higher temperature and light intensity at the medium upland elevation decrease the number of blooming flowers, flower diameter, and red color flower intensity of Chrysanthemum morifoliumcompared to that at the high upland elevation. The environmental modification was needed during the flower development phase to improve the quality of chrysantheum at a medium upland elevation. The study aimed to find the suitable screen house environmental modification for increasing the chrysanthemum quality at the medium upland elevation. The study consisted of two factors. First, environmental modification of a screen house, such as (1) control, (2) the addition of shading net on the rooftop of the screen house, (3) the addition of a misting system in the screen house, and (4) the addition of a combination of shading net on the rooftop and misting system in the screen house. Second, the chrysanthemum varieties, including (1) Yastayuki (white flower), (2) Arosuka Pelangi (yellow flower), and (3) Socakawani (red flower). Experiments were arranged in a completely randomized design with three replications. Data were analyzed by combined ANOVA. The results showed that either the addition of a shading net or misting system reduced the number of flower buds growing. The competition among flower buds was found to be reduced by decreasing the number of flower buds.  Consequently, the number and diameter of the blooming flower increased. The addition of a shading net on top of the screen house was the most suitable environmental modification to increase the chrysanthemum quality at the medium upland elevation.
EFFECT OF LERAK (SAPINDUS RARAK) EXTRACT IN HIGH ROUGHAGE DIET ON RUMEN MICROBIAL PROTEIN SYNTHESIS AND PERFORMANCE OF SHEEP Elizabeth Wina; Stefan Muetzel
Indonesian Journal of Agricultural Science Vol 21, No 2 (2020): DECEMBER 2020
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v21n2.2020.p89-97

Abstract

Secondary compounds containing plant extract as feed additive may improve the performance of livestock consuming high roughage diet. An in vivo trial was conducted to investigate the effect of Sapindus rarak extract (SRE) on ruminal fermentation products, microbial protein synthesis, and growth performance of sheep. Sheep (male, 28 heads) fed high roughage diet were arranged in a completely randomized design with four treatments: addition of SRE to the diet at 0, 4, 8 and 12 g head-1 day-1. The experiment was conducted for 105 days with 2 weeks adaptation period. At the end of the experiment, total faeces was collected for 1 week and rumen liquor was taken. Variables measured were ruminal fermentation products, microbial protein synthesis, daily intake, digestibility, N retention, body weight, and average daily gain. Protozoal numbers were significantly decreased with increasing SRE dose. The ruminal NH3 concentration tended to reduce by SRE addition (P = 0.06). SRE significantly increased propionate and efficiency of microbial protein synthesis from 6.4 to 10.5 g N kg-1 DOMR. SRE significantly improved average daily gain of sheep during the first 70 days of experiment without affecting intake and digestibility. SRE did not alter carcass percentage but tended to lower pancreas and liver weights (P<0.1). In conclusion, SRE has a significant role in partially defaunated rumen microflora, hence, increased microbial protein synthesis and propionate production in the rumen. Addition of SRE is useful to increase daily gain of sheep fed high roughage diet in 70 days of feeding.

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