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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 352 Documents
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POTENTIAL OF ENDOPHYTIC FUNGI DERIVING FROM ASIATIC PENNYWORTH TO PRODUCE ANTIOXIDANTS Dwi Ningsih Susilowati; Nani Radiastuti; Nurhasni Nurhasni; Lisca Puji Rustanti; Ika Roostika Tambunan
Indonesian Journal of Agricultural Science Vol 22, No 2 (2021): DECEMBER 2021
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v22n2.2021.p58-65

Abstract

Asiatic pennyworth is a medicinal plant that contains triterpenoids, saponin, flavonoids, and tannins which possess antioxidants. Endophytic fungi from the plant could produce a similar compound; therefore, antioxidants could be made in the laboratory if the fungi are isolated. This study aimed to evaluate the potential of endophytic fungi isolated from Asiatic pennyworth to produce antioxidants. The study used 34 endophytic fungal isolates from Asiatic pennyworth accessions of Malaysia (17 isolates) and Bengkulu, Indonesia (17 isolates) collected by the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. The fungi were propagated in a potato dextrose broth medium, then mycelia mats and filtrates were separated and then freeze-dried. The antioxidant activities were first tested with 1,1-diphenyl-2- picrylhydrazyl (DPPH) using thin layer chromatography (TLC), then UV-Vis spectrophotometry λ517 nm with five variations of concentration. Results showed all 34 fungal isolates have antioxidant activities based on a yellowish-white color change after applying 0.002% DPPH solution of the TLC method and IC50 value of the UV-Vis spectrophotometer. The highest antioxidant activity was shown by Aspergillus austroafricanus MB 1 (IC50 = 12.08 ppm) from Bengkulu accession and A. oryzae MM 13 (IC50 = 10.29 ppm) from Malaysia accession. A. austroafricanus MB 1 produced more antioxidant compounds (seven) than A. oryzae MM 13 (six). The antioxidant compounds produced by both endophytic fungi included in the group of flavonoids, fatty acids, and carboxylic acids. The research implies that A. austroafricanus MB 1 and A. oryzae MM 13 could be further developed as sources of antioxidants.
GENETIC ANALYSIS OF QUALITATIVE AND QUANTITATIVE TRAITS AMONG PROGENIES OF ENSET CLONES ORIGINATED FROM SOUTHERN ETHIOPIA Abraham Bosha; Mesfin Kebede Gessese
Indonesian Journal of Agricultural Science Vol 22, No 2 (2021): DECEMBER 2021
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v22n2.2021.p66-76

Abstract

The present cultivated enset (Ensete ventricosum) clonal landraces in Ethiopia originated from few wild progenitors. However, enset has a mixed mode of reproduction in which, the wild enset reproduces sexually through seeds, while cultivated enset is generally propagated vegetatively. The objectives of this study were to determine the genetic structure of enset cultivars through genetic analysis of qualitative morphological traits and estimate their genetic variability by evaluating the quantitative agronomic traits data generated from progenies of cultivated and wild enset genotypes. Hence, seeds collected from six cultivated and four wild enset genotypes were used for this study. Data on four qualitative and six quantitative morphological traits were recorded from the progenies of the 10 enset genotypes. Progenies of seven enset genotypes segregated in a 3:1 segregation ratio while progenies of the remaining genotypes segregated differently for the qualitative traits considered. With regard to the quantitative traits, the progenies of the 10 enset genotypes differed significantly for five of the six traits except pseudostem length. The cultivated clones, in general performed better than that of the wild types. Moderate heritability (h2b) estimates and high genetic advances were obtained for leaf length (0.38, 62.0%), pseudostem circumference (0.35, 78.5%), and plant height (0.30, 19.1%) indicating selection for these traits indirectly improves economic yield of enset clones. This study demonstrated the possibility of creating genetic variation through selfing the existing enset genotypes for traits of interest and making improvements either through selection or crossing the elite types to develop novel enset cultivars.

Page 36 of 36 | Total Record : 352

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