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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 10, No 2 (2014): Agustus" : 5 Documents clear
Keragaman Genetik Kultivar Padi Beras Hitam Lokal Berdasarkan Penanda Mikrosatelit Kristamtini Kristamtini; Taryono Taryono; Panjisakti Basunanda; Rudi H. Murti
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p69-76

Abstract

Indonesia has diverseaccessions of local black rice, which are important sourcesof germplasm. However, some of the local black ricecultivars have different names, leading a need to beidentified to determine their genetic diversity usingmolecular marker. This study aimed to identify geneticdiversity of eleven cultivars of local black rice, collection ofthe Assessment Institute for Agricultural Technology,Yogyakarta and compared them with two white ricevarieties using four microsatellite markers. Detection ofmicrosatellite alleles polymorphism was carried out byvisualization of PCR amplicons by electrophoresis onagarose gel. To estimate their genetic diversity, phylogenetictree and principal coordinate analysis were performed usingbinary data of SSR alleles. The results revealed that totalmarkers enabled to differentiate black rice cultivars asreflected by high value of polymorphic information content(PIC) mean (0.695). This value was consistent with the highgenetic diversity of black rice (genetic diversity index, h =0.283) in comparison with white rice cultivars (h = 0.020).The phylogenetic and main coordinate analyses suggestedthat black rice cultivars genetically differed from white rice.The local black rice cultivars were preferentially groupedbased on their genetic those were distributed in threecoordinates and did not represent their local geographicorigin. Genetic diversity analysis in this study will be usefulas an initial basis for proper identification and selection forappropriate parents to assist breeding program of black ricein Indonesia.
Pengaruh Media terhadap Produksi Prodigiosin Isolat Bakteri Entomopatogen Serratia marcescens Asal Wereng Batang Cokelat Ifa Manzila; Tri P. Priyatno; Rahminovita Herlis; Iman Rusmana; I Made Samudra; Yadi Suryadi
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p77-84

Abstract

Prodigiosin, the red pigment producedby the bacterium Serratia marcescens, is a secondarymetabolite of the family tripyrrole that has been widely usedas an antibiotic in the multifunction treatment ofantibacterial as well as antifungal. This study was aimed tostudy the effect of Luria-Bertani (LB) broth and nutrientbroth (NB) media suplemented with several concentrationsof FeSO4 and CaCO3 on the production and characteristic ofprodigiosin derived from S. marcescens. The study wasarranged in a completely randomized factorial design withfour replications. The LB and NB media were supplementedwith 0, 2.5, 5, and 10 mM CaCO3 and 0, 0.25, 0.5, and 1 mMFeSO4. Results showed a red pigment produced by S.marcescens when cultured on both LB and NB media. Redlikepigmentation was varied when supplemented withdifferent concentration of Fe2+ and Ca2+. The higher theconcentration of Fe2+, the more intense the red color,conversely, the higher the concentration of Ca2+, the lighterthe red color. The interaction was found between the mediaand concentrations of CaCO3 and FeSO4 on the productionof prodigiosin. The highest prodigiosin production wasobtained on NB media supplemented with FeSO4.Meanwhile, the addition of CaCO3 did not affect theprodigiosin production. An addition of 1 mM FeSO4 to LBand NB media produced crude prodigiosin of 486.0 mg/mland 489.0 mg/ml, respectively. Based on purification bycolumn chromatography using silica gel, the prodigiosinproduction on LB and NB media was 378 mg/ml and 450mg/ml, with the purity level of 77.8% and 92%, respectively.Detection of prodigiosin by thin-layer chromatography usingsilica gel showed the red pigment had Rf value of 0.83 andbioautography assay showed there was an antibacterialactivity against Xanthomanas oryzae pv. oryzae.
Kultur Apeks untuk Penyediaan Bibit Unggul Tebu Varietas PS864 dan PS881 Deden Sukmadjaja; Yati Supriyati; Saptowo J. Pardal
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p45-52

Abstract

In vitro culturetechniques have become alternative to help overcome theproblems those are often encountered in the provision ofseeds through conventional means. Micropropagationthrough apex culture in sugarcane has several advantages,such as the produced plants have higher genetic stability,high multiplication rate, and more healthy seeds (especiallyvirus-free)., The aims of the the research were to produceseeds of two varieties of sugarcane, namely PS864 andPS881, through apex culture. Laboratory-scale research wasconducted at the Indonesian Center for AgriculturalBiotechnology and Genetic Resources Research andDevelopment (ICABIOGRAD), Bogor, while sowing seedsnursery was done in the Experimental Station of Gowa,South Sulawesi Assessment Institute for AgriculturalTechnology. The experiments consisted of initiation andregeneration of apexes, shoots multiplication, rootinginduction, and acclimatization of plantlets. Research resultsshowed the initiation and regeneration of PS864 and PS881through apex culture could be done on MS basic mediumcontaining 0.5 mg/l BAP. Shoot proliferation of both varietiesincreased in the second subculture. Addition of 1 mg/l BAPinto medium in the second subculture resulted in higheraverage number of shoots than that of 5 mg/l BAP, both forPS864 and PS881. Addition of 1 mg/l and 5 mg/l kinetinshowed no significant differences for shoot numberscompared to that of PS864 in medium containing 1 mg/lBAP. The average number of PS881 shoots in multiplicationmedia containing 5 mg/l kinetin was higher than that of 1mg/l kinetin. Increased concentrations of NAA and IBA from0.1 mg/l to 0.5 mg/l in the MS medium were correlated to theincreased number of roots in PS864 shoots. Meanwhile, onlyincreased concentration of NAA that affected rooting percentageof PS881. Acclimatization showed the percentage ofthe plantlets grown in polybags was higher than that directlygrown in planting bed. The primary seeds (G0) produced inthese experiments were ready to be reproduced again toobtain further stages.
Multiplikasi Tunas dan Induksi Perakaran pada Ubi Kelapa (Dioscorea alata L.) dan Gembili (Dioscorea esculenta L.) Secara In Vitro Hutami, Sri; Purnamaningsih, Ragapadmi; Mariska, Ika; Diantina, Surya
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p53-60

Abstract

Dioscorea sp. (yam) is one of the minor tuber crops whichgrows wildly in the forest and only a few of its species arecultivated and used as main or secondary food.Conservation is needed to preserve plant genetic material.The objective of this research was to obtain methods ofplantlets propagation of D. alata L. and D. esculenta L.through in vitro culture. The research was conducted atTissue Culture Laboratory of ICABIOGRAD in 2012. Theresearch consisted of three stages. First, shoot emergence.In this experiment, young shoots were planted in MS basicmedium combined with benzyl adenine (BA) (0, 1, 3, and 5mg/l) and gibberelic acid (GA) (0 and 5 mg/l). Second, shootmultiplication. Shoots of Dioscorea which were planted inthe best medium of the first experiment were subcultured inMS medium combined with thidiazuron (0, 0.1, 0.5, 1, 2, and3 mg/l). Third, root initiation. Shoots of Dioscorea whichwere planted in the best medium of the second experimentwere subcultured in MS medium (½ MS and 1 MS)combined with indole-3-butyric acid (IBA) (0, 1, 3, and 5mg/l). Result of these experiments showed that shootemergence of D. alata L. and D. esculenta L. began at 2weeks after planting in MS medium. More plantlets of D.alata L. and D. esculenta L. were obtained by shootmultiplication in MS media. Root initiation of the Dioscoreabegan at 4 weeks after planting in MS media. The addition ofIBA (3–5 mg/l) on D. esculenta L. could not stimulate rootingbut led to the formation of callus at the base of the stembuds.
Konstitusi Genetik dan Karakter Fenotipik Galur-galur Padi Pup1 Turunan Varietas Situ Bagendit Suwaji Handaru Wardoyo; Miftahudin Miftahudin; Sugiono Moeljopawiro; Joko Prasetiyono
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p61-68

Abstract

Acidity, phosphorus deficiency, and droughtstress are major problems in Indonesia’s Ultisol rice farming.Development of rice lines tolerant to those stresses isexpected to be able to reduce the consumption of Pfertilizer. The objectives of the research were to evaluategenetic constitutive of rice lines (BC2F6 population) derivedfrom Situ Bagendit x Kasalath and Situ Bagendit x NIL-C433crossings, and to evaluate responses of those lines toYoshida nutrient solution under P deficiency and Al stresscondition. The research was conducted at Molecular BiologyLaboratory and Greenhouse of ICABIOGRAD, fromNovember 2011 to May 2013. The result of foregroundanalysis showed that Pup1 locus has been integrated intothe genome of BC2F6 rice lines, eventhough some lines (SK5,SK6, SK7, SK8, SK9, SK10, SK19, and SK20) showedincomplete integration. Background analysis indicated thatmajority (95.7%) of the Situ Bagendit background has beenrecovered in BC2F6 rice lines. Al stress evaluation showed SNlines were more tolerant to P deficiency and Al stress thanthat of SK lines. Pup1 locus showed good expression underlow P and no Al stress. Based on genome proportion andYoshida nutrient solution experiments, a total of three lines,namely SK13, SN2, and SN9, have potential goodcharacteristics. Molecular analysis within a marker-assistedbackcrossing (MAB) experiment should be carried out ateach generation of lines for gaining fully gene segment inadvanced generations.

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