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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 15, No 2 (2019): December" : 7 Documents clear
Genetic Diversity Analysis of 41 Chili Pepper Genotypes (Capsicum annuum L.) Based on SSR Markers Wartono Wartono; Suryo Wiyono; Muhamad Syukur; Giyanto Giyanto; Kristianto Nugroho; Puji Lestari
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p65-74

Abstract

Chili (Capsicum annuum L.) is an important horticultural crop which possesses high genetic diversity. Their genetic diversity needs to be assessed through molecular marker-based analysis to facilitate chili breeding scheme. The objective of the study was to analyze the genetic diversity of 41 chili genotypes using Simple Sequence Repeat (SSR) markers. Eleven SSR markers were used to amplify all genotypes through PCR technique, visualized on Polyacrylamide Gel Electrophoresis (PAGE). The data were analyzed using NTSYSpc version 2.1 and PowerMarker version 3.25 softwares. Results showed that the average of SSR alleles was 7.9 with a range of 4-11 alleles per marker. All SSR markers showed PIC value >0.5, indicating their suitable use for chili genetic studies. Cluster analysis of 41 genotypes generated three main groups according to coefficient similarity of 77.4%. The genetic clusters appeared as reflection of the genetic background of each genotype. In addition, the SSR markers used can identify potential parent as indicated by the genetic distance between genotypes. The highest genetic diversity (0.698) was demonstrated by combinations of Yuni × Ayesha, Yuni × Ayesha 2, and Yuni × Namira. The estimated genetic diversity produced by SSR markers in this study should be useful as guidance for chili breeders to select suitable parents for new cultivar development for high productivity and resistance to diseases.
Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers Kristianto Nugroho; Rerenstradika T. Terriyana; Kusmana Kusmana; Puji Lestari; I Made Tasma
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p53-64

Abstract

Potato is one of important carbohydrate sources used as an alternative crop in Indonesia. The challenges in national potato breeding program included low productivity, less tolerance to environmental stresses, and narrow genetic diversity. The purpose of this study was to analyze genetic diversity of 14 potato genotypes based on morphological characters and SSR markers newly developed from genome sequences of Indonesian potato genotypes. Principal component analysis of morphological data was done using program XLSTAT. DNA of 14 potato genotypes were assayed using 22 SSR markers. Phylogenetic tree was constructed using program NTSYS version 2.1. The PCA showed that leaf shape, leaf color, tuber shape, tuber skin color, and tuber color contributed most to the total diversity. SSR polymorphism analysis demonstrated that as many as 196 alleles were detected in this study. The average allele number was 8.9 ranged from 5 to 13 alleles per locus. The average major allele frequencies was 22% ranged from 14 to 43%. Gene diversity ranged from 0.70 to 0.92 with the average of 0.86, meanwhile the heterozigosity observed ranged from 0 to 0.71 with the average of 0.05. Phylogenetic analysis generated two main clusters in the coefficient of similarity 0.77. The first cluster consisted of three genotypes while the second cluster consisted of eleven genotypes. The new developed SSR markers used in this study were able to differentiate potato accessions having similar morphological characters but were different genetically. The results of this study should be useful in assessing genetic materials in potato cultivar development program.
Increasing the Growth and Quality of Red Chili with Growth Hormone from Endophytic Bacteria Rafika Yuniawati; Siti Fatimah; Reni Indrayanti; Ifa Manzila; Tri Puji Priyatno; Dwi Ningsih Susilowati
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p75-82

Abstract

Red chili is a very important horticultural commodity in Indonesia having low productivity and quality. Cultivation method needs to be improved including the use of exogenous growth hormones. The purpose of this study was to determine (1) the optimum concentration of IAA and GA growing hormones from isolate B6.2 in stimulating plant growth and improving the quality of large red chili fruit; (2) molecular identity of the B6.2 bacterial isolate. The growth hormone content of B6.2 isolates using HPLC obtained 0.49 ppm IAA and 64.53 ppm GA. The growth hormone potential test on the growth and quality of chili was carried out with a concentration of 1, 3, 5 ml/l, while water and synthetic hormones was used as negative and positive control, respectively. The experimental design used was a Complete Random Design with the foliar spray application to the plant canopy three times during the growth period. The results showed the best concentration in increasing plant height, fruit weight, shooth wet, and dry weight compared to controls at the age of 76 days after planting (dap) was a concentration of 5 ml/l, with the values of 71.7±0.9 cm , 94.7±0.3 g, 11.5±0.43 g, and 1.4±0.09 g, respectively. The molecular identification showed that B6.2 isolate was classified as Bacillus vallismortis with 100% homology. The growth hormone from isolate B6.2 has the potential to increase growth and production of red chili plants.
Molecular Analysis and Phenotypic Performances of BC3F2 Upland Rice Lines Containing Alt and Pup1 Loci Mizan, Muhammad Rauful; Wirnas, Desta; Tasliah, nFN; Hidayatun, Nurul; Prasetiyono, Joko
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p83-92

Abstract

The challenges in upland rice cultivation are phosphorous (P) deficiency and aluminum (Al) toxicity, each controlled by Pup1 and Alt loci, respectively. Pyramiding the two genes into Indonesian rice varieties were previously done through Marker-Assisted Backcrossing method to obtain BC3F2 populations. The aims of this study were to analyze the BC3F2 upland rice lines containing the Alt and Pup1 loci molecularly (foreground and background analyses) and to test their phenotypic performances. Genetic materials tested included Dupa variety (donor of Alt) and three improved Indonesian genotypes (Dodokan-Pup1, Situ Bagendit-Pup1, and Batur-Pup1) as recurrent parents, Kasalath (donor of Pup1), and 300 BC3F2 lines from Dodokan-Pup1+Alt, Situ Bagendit-Pup1+Alt, and Batur-Pup1+Alt, respectively. The rice genotypes were selected individually using modified Yoshida nutrient solution, followed by foreground and background analyses. 150 out of 300 seedlings were selected and maintained until harvest in the greenhouse. Foreground analysis using markers (RM1361, RM12031, and Kas46-2) and tiller number performances resulted in 18 plants from BC3F2 Dodokan-Pup1+Alt, 30 plants from BC3F2 Situ Bagendit-Pup1+Alt, and 25 plants from BC3F2 Batur-Pup1+Alt still carrying Alt and Pup1 loci. Background analysis using molecular markers showed that the best individual lines of BC3F2 were number 56 for BC3F2 Dodokan-Pup1+Alt, number 32 or 70 for BC3F2 Situ Bagendit-Pup1+Alt, and number 20 for BC3F2 Batur-Pup1+Alt. The selected lines having both both Alt and Pup1 loci in homozygote condition with highest number of tiller per plant which are useful genetic materials for developing upland rice variety tolerance to low P and Al toxicity.
Fron Matter JA Vol 15 No 2 AgroBiogen, Jurnal
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p%p

Abstract

Back Matter JA Vol 15 No 2 Jurnal AgroBiogen
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p%p

Abstract

Genome-Edited Plants and the Challenges of Regulating Their Biosafety in Indonesia nFN Bahagiawati; Dani Satyawan; Tri J. Santoso
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p93-106

Abstract

Genome editing is a precise breeding technique to improve plant properties by editing specific genes that regulate desired trait. Genome editing techniques can be designed so that the resulting plant does not contain foreign genes and the resulting changes in DNA sequences cannot be distinguished from products obtained by conventional gene mutations which have been considered as safe and therefore unregulated. Thus, genome editing products in some countries are also not specifically regulated as GM products even though their assembly process uses recombinant DNA and genetic transformation. Brazil, likeIndonesia ratified the Cartagena Protocol, but it issued a special  regulation that provides dispensation for several types of genome editing products and exempts them from regulations that apply to transgenic plants. The steps taken by other countries in regulating genome editing products can be taken into consideration in drafting regulations in Indonesia, in order to create a conducive environment that supports the use of this potential technology while at the same time provides assurance regarding its safety to human health and the environment. The purpose of this review was to provide information onthe development of genome editing technologies in plant breeding, analyze its risks compared to that of conventional breeding, and compare its biosafety regulation in various countries to provide some considerations for drafting regulations on the risk assessment of genome editing products in Indonesia, as a ratifying country of the Cartagena Protocol.

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