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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 6 Documents
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Search results for , issue "Vol 5, No 2 (2009): Oktober" : 6 Documents clear
Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi cv. Asemandi dengan bantuan Agrobacterium tumefaciens Atmitri Sisharmini; Aniversari Apriana; Wening Enggraini; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p49-56

Abstract

The rice transformation technologyis not only provides valuable methods for the introductionof useful genes into rice plant to improve importantagronomic traits, but also helps in studying gene functionand regulation based on rice genome sequence information.Knockout of genes by insertional mutagenesis is a straightforwardmethod to identify gene functions. One of themethods to develop rice mutants is through genetic transformationmediated by Agrobacterium using activationtagging by Ac-Ds system. A study was done with an objectiveto obtain mutant rice of local tropical japonica cv. Asemandithrough genetic trans-formation mediated by Agrobacteriumtumefaciens. The transformation was conducted usingAgrobacterium vector with the strain of Agl-1 containingactivation tag construct. The result of experiment showedthat it has been obtained 17 independent line (304 plants)transgenic Asemandi containing activation tag construct.These starter lines will be used as materials to developseveral generations of stabil rice mutant through selfing.
TINJAUAN Penggunaan Suspensi Sel dalam Kultur In Vitro Sri Hutami
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p84-92

Abstract

Cell suspension culture could be defined as aprocess that allows rapidly dividing homogenous suspensionof cells to grow in liquid nutrient media. There are two maintypes of suspension cultures: (1) Batch cultures in whichcells are nurtured in a fixed volume of medium until growthceases and (2) Continuous cultures in which cell growth ismaintained by continuous replenishment of sterile nutrientmedia. Plant cell suspension cultures are mostly used for thebiochemical investigation of cell physiology, growth, metabolism,protoplast fusion, transformation and for large scaleproduction of seed by bioreactor and production of secondarymetabolites. Contamination is one of the largest problemswhen dealing with cell cultures. Differences betweenthe products of cell suspension culture and whole plant arefrequently observed. These phenomena’s may be resultedfrom lack of differentiation and organization and cell cultureinducedvariation. Utilization of cell suspension culture inIndonesia is still limited, some of them for mass productionof plantation seed with bioreactor system and for productionof secondary metabolites. The success of this study give theopportunity for mass production of seeds from other plantsand also production of secondary metabolites.
Pengembangan Set Multipleks Penanda DNA Mikrosatelit untuk Analisis Variasi Genetik Padi dan Kedelai Chaerani Chaerani; Nurul Hidayatun; Dwinita W. Utami
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p57-64

Abstract

Detection of multiplex microsatellite markers in asingle capillary array on a laser detection system is traditionallyconducted with specific primers that are labelled withfluorescent dyes. An alternative method using fluorescentlabels that are appended to 5’ end of universal primer M13instead of to the specific primers offers flexibility indesignning multiplex panels and a less expensive method.Allele size range of microsatellite loci that can be grouped inmultiplex panels can be accurately estimated by pooling andanalyzing DNA samples from several genotypes simultaneously.This paper describes the procedure in developmentof microsatellite multiplex panels using M13 fluorescentlylabelledand estimation of allele size range based on pooledDNA strategies. Two multiplex panels of PCR amplificationproducts for rice consisting of 15 loci and three panels forsoybean consisting of 10 loci have been designed. Thepanels have been applied to 50 accessions of rice and soybeanwith fairly good results. Further characterization ofallele size range, however, is required prior to the applicationof these panels to diverse genotypes. The proceduredescribed here should be applicable in the development ofmultiplex panels of other species.
Analisis Keragaman Genetik Acremonium yang Berasosiasi dengan Tanaman Gaharu Menggunakan Teknik Random Amplified Polymorphic DNA (RAPD) Dewi Rahmawati; Nurita Toruan-Mathius
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p65-70

Abstract

Agarwoodor gaharu is a plant that has a high economic value in Asia,due to its use for production of incense and traditionalmedicines. The agarwood formation occurs in the trunk androots of trees that have been infected by a fungus, such asAcremonium spp. Various fungi were associated with theagarwood formation. Acremonium is generally considered ashighly polyphyletic, contains distantly related fungi. A studywas done to identify genetic diversities in 10 isolates ofAcremonium spp. from four different areas in Indonesia thatare associated with Aquilaria and Gyrinops verstigii using theRandom Amplified Polymorphic DNA (RAPD) technique.Eight RAPD primers, i.e., OPA 02, OPB 04, OPB 07, OPB 17,OPC 11, OPD 03, OPD 05, and OPE 07 were used in theanalyses. The results indicated that similarity index values ofthe genetic variation ranged from 0.21 to 0.97. Based on theNei and Li’s similarity coefficients, these values indicatingthe presence of high degree of genetic variability. The lowestdegree of genetic similarity were found between isolates F(Acremonium spp., which is associated with G. verstigii fromMataram, Nusa Tenggara Barat), and LM2 from south coastalarea of West Sumatra. The highest genetic similarity value(0.97) was found between isolates Sr2 and Sr4 from Sorong,Papua. Results from the cluster analysis indicated that theisolates could be grouped into two major clusters that wereassociated with their geographical locations.
Hubungan Kekerabatan Jamur Pelapuk Putih Pleurotus spp. dengan Analisis Isoenzim Achmad Achmad; Elis N. Herliyana; Ficky R. Agustian
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p78-83

Abstract

Pleurotus spp. is anedible mushroom commonly found on stem of wide leaftrees or other wooden plants in the forest. A number ofPleurotus species are found in Indonesia, and some of themhave been cultivated. An accurate technique is needed toidentify the species of Pleurotus correctly; one of the methodis the isoenzyme technique. Apart from its simplicity, cheapnessand quickness, this method also gives accurate informationon phylogenetic relationship among the Pleurotusspp. The technique was used to determine phylogeneticrelationship in 6 isolates of Pleurotus spp., i.e., Pleurotussp.8, Pleurotus sp.6, Pleurotus sp.1, Pleurotus sp.7, andPleurotus sp.9, using the GOT System. Results of the analysisindicated that all the Pleurotus isolates tested produced twobands with similar thickness, except for Pleurotus sp.1 thatproduced one band that move to the cathode (-). Anotherisolate of Pleurotus spp. produced bands tend to move to theanode (+). The genetic distances between Pleurotus sp.8was similar to that of Pleurotus sp.9, while that of Pleurotussp.6 was similar to Pleurotus sp.7. Genetic distances ofPleurotus sp.8 or Pleurotus sp.9 was similar to Pleurotus sp.6or Pleurotus sp.7, with the longest distance on Pleurotussp.1. Pleurotus sp.1 showed a different migration distance,where one of the isoenzym band tend to move to thecathode (-). This indicated that Pleurotus sp.1 has differentphylogenetic relationship with the other Pleurotus spp.
Rice Anther Culture to Develop Double Haploid Population and Blast Resistant Lines Ambarwati, Dinar; Soemantri, Ida H.; Utami, Dwinita W.; Apriana, Aniversari; Moeljopawiro, Sugiono
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p71-77

Abstract

Penyakit blas pada padi yang disebabkanoleh cendawan Pyricularia grisea, merupakan salah satukendala dalam produksi beras. Sumber gen ketahanan terhadappenyakit blas dijumpai pada spesies padi liar Oryzarufipogon. Populasi silang ganda (BC2F3) turunan IR64 danO. rufipogon mempunyai QTL untuk sifat ketahanan terhadappenyakit blas. Untuk mempercepat perolehan tanamanhomosigot dari populasi tersebut, dilakukan kultur anterpada dua media induksi kalus: I1 (N6 + NAA 2 mg/l + kinetin0,5 mg/l + sukrosa 60 g/l + putresin 0,16 g/l) dan I2 (N6 +2,4-D 2 mg/l + sukrosa 50 g/l) dan dua media regenerasi: R1(MS + NAA 0,5 mg/l + kinetin 2 mg/l + sukrosa 40 g/l +putresin 0,16 g/l) dan R2 (MS + NAA 1 mg/l + kinetin 2 mg/l+ sukrosa 30 g/l). Kultur anter dilakukan pada sembilan genotipe,di mana tiga genotipe (149-16, 343, 337-13) memberikanrespon terbaik dalam produksi planlet hijau setelahdikulturkan pada media regenerasi R1. Dari 208 planlet hasilregenerasi diperoleh 42 planlet haploid ganda dari genotipe149-16, 11 planlet haploid ganda dari genotipe 343, dan 44planlet haploid ganda dari genotipe 337-13. Skrining ketahananblas di rumah kaca pada populasi haploid gandamenghasilkan 46 tanaman tahan terhadap ras 001, 33 tanamantahan terhadap ras 033, dan 79 tanaman tahan terhadapras 173. Sebanyak 28 tanaman bersifat tahan, baik terhadapras 001, 033, maupun 173 seperti halnya O. rufipogon.Galur-galur homosigot ini akan diuji di lapang untuk ketahanannyaterhadap penyakit blas dan karakter agronominya.

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