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Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
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Articles 3 Documents
 1 
Search results for , issue "Vol. 4 No. 2 (2017)" : 3 Documents clear
Aktivitas Antioksidan Ekstrak Etanol dari akar, batang, dan daun Sereh Wangi (Cymbopogon nardus L) Waras Nurcholis; Hartanti Hartanti; Hyakansa Hyakansa; Maria Magdalena; Tubagus Iqbal Maulana
Current Biochemistry Vol. 4 No. 2 (2017)
Publisher : IPB University

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Abstract

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Development of Trichodermin Nanoemulsion Based on Medium Chain Triglycerides as Antifungal of Ganoderma boninense in vitro Muhammad Alwin Azhari; Ike Wahyuni Putri; Ahmad Irvan Pratama; Radika Evita Hidayah; Laksmi Ambarsari
Current Biochemistry Vol. 4 No. 2 (2017)
Publisher : IPB University

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Abstract

Trichodermin has antifungal activity against Ganoderma boninense, but it is volatile and insoluble in water. Research on trichodermin as antifungal G.boninense has been done, but not yet at the stage of altering the nature of the compound. This study aims to develop trichodermin as antifungal G. boninense in the form of nanoenkapsulat with observed aspects including formulation, encapsulation, characterization, and antifungal activity. Trichodermin is extracted from Trichoderma harzianum by maceration. Nanoemulsion is made using Emulsion Inversion Point (EIP) method and encapsulation is done by spray drying. Antifungal activity was tested using a modified dual culture assay method. The results showed the rendement of trichodermin extraction of 0.70%. The best nanoemulsion formula is nanoemulsion with Tween 80 concentrations of 75% of the total organic phase. The formula nanoemulsi has a size of 231.95 nm, polydispersity index of 0.348, and zeta potential of -35.6 mV. Encapsulation has a yield of 5.79%. The encapsulated powder has a moisture content of 5.79%, contains a typical functional group of trichodermin compounds, and is round with an uneven surface. The antioxidant activity of nano-trichodermin encapsulation is higher than trichoderminextract and positive control (hexaconazole) with PIRG value of 84.40%.
Pemanfaatan Bakteri Sebagai Pereduksi Emisi Gas Metana Pada Limbah Cair Kelapa Sawit (Elaeis guineesis Jacq.) Nisa Widya Amanda; I Made Artika; Iman Rusmana
Current Biochemistry Vol. 4 No. 2 (2017)
Publisher : IPB University

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Abstract

Kelapa sawit merupakan salah satu tumbuhan penghasil minyak terbesar di Indonesia. Proses pengolahan buah kelapa sawit menjadi minyak memiliki dampak negatif, yakni terciptanya gas metana. Gas metana merupakan salah satu gas penyumbang efek rumah kaca yang menyebabkan pemanasan global. Oleh karena itu, diperlukan cara untuk menurunkan emisi gas metana. Tujuan penelitian ini adalah penggunaan bakteri untuk menurunkan emisi gas metana pada limbah cair pemrosesan buah kelapa sawit. Bakteri diisolasi dari limbah cair kelapa sawit. Terhadap isolat tunggal yang diperoleh dilakukan uji aktivitas enzim soluble methane monooxygenase (sMMO) dan particulate methane monooxygenase (pMMO). Isolat dengan aktivitas methane monooxygenase tertinggi dipilih untuk analisis sekuen DNA dan konstruksi pohon filogenetika. Tahap isolasi bakteri menghasilkan 13 isolat koloni tunggal. Uji aktivitas methane monooxygenase menunjukkan bahwa semua isolat tidak memiliki aktivitas sMMO. Sebaliknya, semua isolat memiliki aktivitas pMMO dengan tingkat berbeda yaitu dengan rentang nilai antara 0.10 – 0.22 M/mL kultur/hari. Analisis sekuen DNA menunjukkan bahwa isolat terpilih adalah Klebsiella sp. Berdasarkan uji Gram diketahui bahwa bakteri ini termasuk bakteri Gram negatif. Bakteri berpotensi digunakan untuk menurunkan emisi gas metan pada limbah cair pemrosesan buah kelapa sawit. Kata kunci: Klebsiella sp, 16S rRNA, Metana, pMMO, sMMO.

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