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Current Biochemictry
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Current Biochemistry
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
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Articles 2 Documents
Search results for , issue "Vol. 8 No. 2 (2021)" : 2 Documents clear
Potency of Bioactive Compounds in Indramayu Mango Peel Waste to Inhibit ACE2 Mustika Luthfia; Assifah Eryandini; Deki Geraldi; Chelsy Narita; Choirunnisa Miftahul Jannah; Laksmi Ambarsari
Current Biochemistry Vol. 8 No. 2 (2021)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.8.2.1

Abstract

According to reports, mango peel contains bioactive compounds—especially phenolic—threefold higher than the flesh. This issue underlies the research that aims to identify bioactive compounds from Indramayu mango peel which have the potential as ACE2 inhibitors. The study was initiated by extracting mango peel simplicia using aquadest, then carried out with phytochemical screening, and identification of compound content by LC-MS. The extraction obtained 21.3% of yield. The results of phytochemical screening showed that secondary metabolites in the form of alkaloids, flavonoids, saponins, and tannins were identified in the mango peel. At the same time, the LC-MS fragmentation results obtained 40 compounds based on the largest sample area. The result from fragmentation were tested for Lipinski bioavailability prediction and ADMET test and obtained 10 potential compounds. Molecular docking was performed on the ACE2 receptor with 10 sample ligands and 1 comparison ligand using YASARA Structure. All of the sample ligands (from Lipinski and ADMET test) showed higher free energies than comparison ligand, chloroquine. Mangiferin is a typical mango compound identified in LC-MS, had inhibitor activity against ACE2. Two-dimensional visualization using Discovery Studio showed mangiferin interaction with the receptor via hydrogen bond, hydrogen carbon bond and phi-anion interactions at three amino acid residues (Thr371, Glu375, Glu402) on the active site of ACE2. Keywords: ACE2, bioactive compound, mango peel, mangiferin, waste
Analysis of Metabolite Compound Profiles of Miana Leaves Endophytic Bacteria (Coleus scutellariodes) using GC-MS Dwi Endah Kusumawati; Ukhradiya Magharaniq Safira Purwanto; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 8 No. 2 (2021)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.8.2.2

Abstract

Exploration of bioactive compounds from endophytic bacteria as novel source of antibacterial need to be explored, as an alternative to substitute synthetic antibiotics. Based on previous studies, DM6 was a potential isolate that showed antibacterial activity. The isolate has been identified as Brevibacillus sp. based on 16S rRNA analysis. This study aimed to identify antibacterial compounds produced by DM6 isolate using GC-MS. The GC-MS results showed that the chloroform extract of DM6 isolate contained : 1-Octadecene (CAS).alpha.-Octadecene, Hexanedioic acid, dioctyl ester (CAS) Dioctyl adipate, Phenol, 3,5-bis(1,1-dimethylethyl)- (CAS) 3,5-Di-tert-butylphenol, and 1,4-diaza-2,5-dioxobicyclo[4.3.0]nonane. Adipic acid/hexanedioic acid and phenols are also known to contained in the leaves of Coleus scutellariodes. This indicated that endophytic bacteria were able to synthesize antibacterial compounds which similar with the host plant.

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