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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 9 Documents

Search results for , issue " Vol 8, No 2 (2003)" : 9 Documents clear

Response of broilers on the diet containing fermented palm oil sludge stored at various length of time Bintang, I.A.K; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

An experiment was conducted to study the response of broilers on the diet containing fermented palm oil sludge with Aspergillus niger stored at different length of time. The research was carried out in Research Institute for Animal Production Ciawi, Bogor. The experiment was conducted based on completely randomized design using 245 day old broiler chicks with 5 replicates. They were allotted to 7 diets containing one control without palm oil sludge (R0), 5 and 10% of fresh-fermented palm oil sludge (LSF) (R1 and R2), 5 and 10% of LSF stored at room temperature for 2 months (R3 and R4) and stored for 3 months (R5 and R6). Diets were fed ad libitum for 5 weeks. Feed intake, weight gain, feed conversion ratio, and carcass yields were observed as parameters. Results showed that no parameters were significantly affected by the treatments. It is concluded that 10% of fermented palm oil sludge could be included in diet of broilers even after three months storage at room temperature. Â Key words: Broiler, fermented palm oil sludge, storage period

Isolation and Screening of Microbes for The microorganism used as probiotics must be bio-safety, could be cheaply and easily Purwadaria, Tresnawati; Kompiang, I Putu; Darma, Jinadasa; ., Supriati; Sudjatmika, Emi
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

produced, and suitable with the environment of the digestive track. Isolation was carried out from commercial culture (containing mixture of Bacillus spp.), digestive tract (proventriculus, small intestine and large intestine) of local and broiler chickens, and commercial yoghurts (fermented milk). Neutral and acidic nutrient agars (NA) were used as the media at room temperature and in the aerobic or anaerobic conditions. Separate colonies were isolated, stained with Gram and spore staining and observed under the microscope. The bacteria which were Gram positive bacillus and can form spores were further identified. Eight different species of Bacillus spp.: B. laterosporus, B. coagulans, B. alvei, B. circulans, B. brevis B. bodius, B. pasteurii, and B. macroides were isolated from the commercial mixture. From the digestive tracts of local and broiler chickens, 13 bacteria and 2 yeasts were isolated, while 5 yeast were obtained from two commercial yoghurts. Those bacteria were facultative aerobic and only grew in neutral condition and not in acidic condition (pH 4.5), while the yeast were either facultative anaerobic also can grow in pH neutral and 4.5. The ability of each isolates to grow in the media containing mixture of minerals and glucose, sucrose or molasses were evaluated. Incubation was carried out in the shaker incubator at 40Â°C, 150 rpm for 48 hours. Dry matter of the biomass was determined as the growth parameter. All isolates of bacteria and yeast can grow in the substrate containing glucose, sucrose and molasses. Variance analyses show that there were interactions between kind of bacteria and carbon source or between kind of yeast and carbon source (P<0.05). Three isolates of bacteria that had highest production of biomass were B. coagulans on sucrose (the third for molasses), Sp. 9 on glucose, and B. apiarius on sucrose (the sixth on molasses). B. apiarius isolated from digestive tract of local chicken will be useful to be developed as probiotics. The highest production of yeast was the control Saccharomyces cerevisiae on three sugars. Between the isolates from digestive tract of local chicken, Toluraspora delbrueckii AL-15 produced 13.8x10-1mg/ml biomassa on molasses. Â Key words: Poultry probiotics, bacterial and yeast isolates, glucose, sucrose, molasses

Effectivity of various concentration of lactose in Tris extender on liquid semen viability of Saanen bucks Tambing, Surya Natal; Sutama, I-K; Arifiantini, R.I
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

The objective of this study is to determine the best concentration of lactose in Tris extender in maintaining liquid semen viability of Saanen bucks stored at 5Â°C. Four heads of Saanen bucks of 2-4 years old were used as semen source. Semen was collected once a week using an artificial vagina. Variance analysis was conducted based on completely randomized design with three treatments, i.e 30, 60, and 90 mM lactose (L30, L68 and L90). Duncan test was used to observe the different between treatment. Results indicated that the mean percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap of liquid semen in treatment L30 (70.83; 78.20; 79.56 and 81.20% respectively) were significantly higher (P<0.05) than treatment L60 (67.08; 73.73; 75.01 and 76.87% respectively) and treatment L90 (61.50; 71.88; 69.08 and 71.33% respectively). After storage for 48 hours at 5Â°C apparently the mean percentage of motility, live sperm, intact plasma membrane and intact acrosomal cap in treatment L30 were significantly higher (P<0.05) than those of in treatment L60 and L90. Decreasing percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap during storage at 50C from 0 until 48 hour in treatment L30 (42.83; 19.75; 40.84 and 39.00% respectively) were significantly lower (P<0.05) than those of in treatment L60 (55.41; 23.06; 50.14; and 50.90% respectively) and treatment L90 (54.83; 29.93; 50.14 and 50.97% respectively). It was concluded that supplementation 30 mM lactose in Tris extender could maintain liquid semen viability in Saanen bucks. Â Key words: Saanen bucks, lactose, liquid semen

The effect of glutathione addition in sperm diluent on the quality of bovine chilled semen Triwulaninngsih, Endang; Situmorang, P; Sugiarti, T; Sianturi, R.G; Kusumaningrum, D.A
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

This study has been conducted at the Laboratory of Physiology Reproduction, Research Institute for Animal Production (RIAP), Ciawi-Bogor, West Java. Sperms were collected from FH bulls with body weight 613 kg (FH-1) and 480 kg (FH-2) twice a week. Briefly after quality evaluation, semen was diluted in Tris-Citrate buffer medium, containing egg yolk (20% v/v) and (4% v/v) glycerol to get spermatozoa concentration of 50 x 106 per ml. Sperm diluents were added with glutathione (GSH) with doses of 0.0; 0.5; 1.0 and 1.5 mM as treatments A, B, C and D respectively. The diluted semen was then cooled from 35 to 5Â°C using a cooling machine for 60 minutes then stored in the refrigerator (5Â°C). Recorded parameters were the survivability of spermatozoa by evaluating the percentage of motile and live, the condition of acrosome and plasma membrane. Data were analysed by completely randomised design with the general linear model (GLM) procedure. The characteristics of collected semen were normal. Viability of spermatozoa stored at 5Â°C for 0, 1, 4 and 8 days shown by intact acrosomal were 74.42; 69.27; 57.80 and 42.58% for A, B, C and D respectively. Those data were significantly different (P<0.01). Motility, live and intact plasma membrane were 46.72; 52.34; 53.44 and 51.09%; 63.59; 69.11; 68.64; and 66.89%, and 66.01; 69.75; 68.38 and 68.44% for treatment A, B, C and D respectively. Additional 0.5 mM GSH gave the highest (P<0.01) motility, live and intact plasma membrane of sperm. Therefore, it is concluded that the effect of addition 0.5 mM of GSH to the sperm diluents can improve the viability of spermatozoa and possibly protect the spermatozoa from free radical damage. Â Key words: Glutathione, viability, spermatozoa

Comparative study of the productivity of Madura Cattle and Its crossbreed with Limousin in Madura island Kutsiyah, Farahdilla; ., Kusmartono; Susilawati, Trinil
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

problem and potential of crossbreed cattle at Madura island. Survey and direct field observation were conducted in Pamekasan. Data were descriptively analyzed or using correlation-regression, and t-test and indicated that service per conception (S/C) and calving rate (CR) of Madura cattle (n=34) and crossbreed (n=38) were 1.464 and 1.986; 58.8 and 52.6% respectively. Average birth weight (n = 17), weaning weight (n = 15) and ADG (average daily gain) pre weaning (n = 15) on calf of Madura cattle were 19.78 Â± 1.224 kg; 119.53 Â± 9.772 kg; 445 Â± 48.53 g/day. Average birth weight (n = 17), weaning weight (n = 14) and ADG pre weaning (n = 14), on calf of crossbreed offspring were 27.60 Â± 1.298 kg; 171.47 Â± 31.055 kg; 678 Â± 146.03 g/day. It is concluded that reproductive performance of Madura cattle was greater than their crossbreed, whereas production performance on calf of crossbreed offspring was greater than calf of Madura cattle. It is suggested that good quality and continuous supply of local feed must be maintained to prevent the negative effect for their growth. Â Key words: Madura cattle, Limousine cattle, crossbreed, productivity

Comparison of sequences of hypervariable region (HVR) subunit S-1 gene of field isolate I-37 infectious bronchitis virus with Connecticut serotype Dharmayanti, N.L.P Indi; Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Infectious Bronchitis is a contagious and acute respiratory disease in chickens caused by infectious bronchitis virus (IBV).Antigenic differences in IBV are associated with changes in the sequence of the spike glycoprotein (S). The subunit S1 which demonstrates more sequence variability than S-2 have been identified as hypervariable region (HVR-1 and 2). There were several IB virus field isolates included I-37 have been identified in Indonesia by serum neutralization method. However, gene sequence variation in HVR subunit S-1 had not yet been identified. Isolate I-37 was close to the serotype Connecticut 46 (Conn 46). The aim of this study is to identify sequence variation of HVR subunit S-1 gene of isolate I-37 produced by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing. Several procedures were carried out in the study including virus titration, propagation and was concentrated from the allantoic fluid infected with IBV. Then, RNA was extracted for RTPCR. urther the product was sequnced and its homology with IBV references from GenBank was compared by GenMac version 8.0. Result showed that isolate I-37 produced 515 bp of amplification product. Isolate I-37 and Conn 46 are same serotype, yet their HVR subunit S-1 nucleotides and amino acids (protein) differ by 6.9% and 15.6% respectively. It might be concluded that isolate I-37 was variant of Conn 46. Â Key words: Sequences variation, IBV, I-37 field isolate, HVR subunit S-1 gene

Histopathological changes in naive and sensitised goats caused by Sarcoptes scabiei infestation Tarigan, Simson
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

The purpose of this study is to compare the histopathological changes in naÃ¯ve and sensitised goats caused by Sarcoptes scabiei infestation. Thirty goats were allocated evenly into 5 groups. Groups 1, 2 and 3 goats were sensitised once, twice and thrice, respectively; whereas groups 4 and 5 were left unsensitised or naÃ¯ve. Sensitisation was done by infesting the animals with the mite, then 7 week afterwards the animals were completely cured from the mange. After the sensitisation, all, except group 5, goats were infested on both auricles each with approximately 2000 life mites. Biopsies were collected from each group at 2 day then at weekly intervals from 1 to 7 weeks following infestation. The samples were routinely processed, paraffin blocked, and tissue sections were stained with Haematoxyllin and Eosin (H & E), Giemsa, Carbol Chromatrope, and Gramâs when indicated. Lesions in the naÃ¯ve goats developed progressively characterised by thick parakeratotic crusts honeycombed with tunnels containing large number of mites. Lesions in sensitised goats, which were different qualitatively to those in naÃ¯ve goats, developed rapidly characterised by copious amount of serocellular exudates in and on the surface of the epidermis, and marked oedema and cell infiltrations in the dermis. Dermal infiltration by eosinophils, which was rare in naÃ¯ve goats, was apparently an important feature in the sensitised goats. Lesions developed in the sensitised goats were interpreted to be the manifestation of cutaneous anaphylaxis. Resistance or protective immunity against mite reinfestation developed in the sensitised goats is supposedly attributed to this anaphylactic responses. Â Key words: Sarcoptes scabiei, naÃ¯ve, sensitised, histopathology, eosinophils, cutaneous anaphylaxis, protective immunity

Isolation and identification of infectious laryngotracheitis virus from outbreaks at Lipa City, Batangas, Philippines Saepulloh, Muharam; Rovira, Hope G
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Infectious laryngotracheitis (ILT) is an acute, highly contagious respiratory disease of poultry characterized by respiratory disorder such as coughing with blood exudate from the trachea. The disease is caused by Herpesvirus of the family Herpesviridae and subfamily of Alphaherpesvirus. ILT is worldwide distribution and has been reported to be present in the Philippines since 1980. Since then, confirmation of subsequent outbreaks were not reported. Isolation was conducted from nine commercial layer chicken farms located at Lipa City, Batangas from May to July 2002. Tracheal and lung extracts were processed and inoculated into embryonated chicken eggs by chorio-allantoic membrane (CAM) inoculation. Five samples produced typical pock lesions in CAM after the second passage. Lesions observed were yellowish pocks with opaque edges, distributed throughout the CAM. A vaccine strain of the virus used as the positive control also produced similar pock lesions. Serological confirmation using the Agar Gel Immunodiffusion (AGID) test showed sharp precipitation lines reacting to a standard reference ILTV antisera (anti-NS175). All five isolates produced lines of precipitate identity among themselves and the positive control. This study confirms that the 2002 disease outbreak in the commercial layer chicken farms in Lipa City, Batangas was due to the ILTV. Â Key words: Infectious laryngotracheitis virus, agar gel immunodiffusion, isolation, pocks lesion, identification, CAM

Characteristics of reproductive performance of Garut rams Rizal, Muhammad; Toelihere, M.R; Yusuf, T.L; Purwantara, B; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Basic information on reproductive potency of Garut rams is necessary in order to identify the capacity of rams in producing chilled or frozen semen. Eight Garut rams (three to five years old) were used in this study. The male sexual behaviors were observed and semen was collected once a week using artificial vagina. Semen quality was evaluated and its potency to produce frozen semen was calculated. Results of this study indicated that first, second, and third ejaculations were at the 29, 87 and 176th seconds, respectively. Fresh semen volume, sperm concentration, motility, intact acrosomal cap, and intact plasma membrane were 0.99 ml, 3224 million/ml; 76.67; 86.13 and 87.73%, respectively. Protein value, fructose, vitamin C, vitamin E, sodiu, potassium, calcium, magnesium, phosphor, chloride, and mangan in seminal plasma of fresh semen were 4140, 180, 3.2, 24, 180, 117, 9, 6.12, 60, 104, and 5 mg/ml, respectively. Measurement of head length, width, and length of sperm tail were 6.59, 3.99, and 42.65 Î¼m, respectively. Length and width measurement of right and left testes, and scrotal circumference were 12.71, 6.5, and 32.36 cm, respectively. Capacity of each Garut rams to produce frozen semen from three consecutive ejaculations are 35.88 mini straw with the cencentration of 200 million motile sperm per 0.25 ml. Â Key words: Reproductive characteristics, Garut rams Â

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Ahmadi Riyanto

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Kota bogor,

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA