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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 8 Documents

Search results for , issue "Vol 20, No 4 (2015): DECEMBER 2015" : 8 Documents clear

Endemicity of avian influenza in ducks living around commercial layer farms Simson Tarigan; Risa Indriani; . Sumarningsih
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The progenitors of all avian influenza viruses are generally derived from ducks or other waterfowl that have undergone mutation and adaptation to become pathogenic in chickens or other poultry. Investigation of the presence of avian influenza viruses in ducks especially those living around chicken farms is, therefore, important. Serum from 54 ducks and 51 Muscovy ducks living around commercial layer farms in the districts of Cianjur and Sukabumi were collected in March - April 2014. The indication of AI-virus infection in those birds was based on an array of serological tests including competitive and indirect ELISAs for antibody to nucleoprotein, MM2e ELISA for antibody to M2e, HI test, ELISAs and dot blot for antibodies to haemagglutinin, and dot blot assay for antibodies to neuraminidase. Recombinant Haemagglutinins (H1-H13 and H15), recombinant neuraminidases (N1, N2, N7 and N9) and recombinant influenza-A nucleoprotein were used in the indirect ELISAs and dot blot assays. As many as 63% of duck samples and 13% Muscovy-duck samples were serologically positive to nucleoprotein, and 62% of the nucleoprotein-seropositive ducks were also positive to M2e. The high seroprevalence of AI in the ducks living around commercial poultry farms suggested that application of strict biosecurity measures on those farms is still needed. Based on the results of the ELISA and dot blot assays, AI virus subtypes H9N2 and H5N2, in addition to H5N1, were suspected to be circulating in those ducks. Further confirmation by virus isolation, however, is required because H9N2 and H5N2 subtypes have yet been unknown Indonesia and both the subtypes can cause serious disease in poultry.Key Words: Duck, Immunoassay, Avian Influenza, H5N1, H5N2, H9N2

Microencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerol Diana Andrianita Kusumaningrum; Bambang Purwantara; Tuti L. Yusuf; Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media. The effect of alginate in microencapsulation process and glycerol concentration on viability spermatozoa (motility (%M), live sperm (%L) and intact apical ridge (%IAR) were studied in two steps. In the first step, the effect of alginate and microencapsulation process was studied. Three treatments of this step were: 1) TCEY add with semen (as control), 2) TCEY-Alginate add with semen, 3) TCEY-Alginate add with semen and microencapsulated, followed by conventional sperm cryopreservation using TCEY 7% glicerol. The second steps were done to evaluate the effect of two glycerol concentrations (5 and 7%) and two duration of equilibration time (3 and 4 hours). Result of these experiments showed, that microencapsulation was significantly alter the percentage of post-thawing motility (%M) and intact apical ridge (%IAR). The motility of microencapsulated sperm vs control was 44.7 and 50.83% respectively, whereas IAR 79.33% and 83.50% on microencapsulated sperm and control. There was evidence that alginate act as extracelullar cryoprotectant by protecting sperm during freezing. The mean of decreasing percentage of %M and %IAR in TCEY-Alginate were lower (15.97 and 6.44%) than control (23.80 and 7.37%). The effect of glycerol concentration and equilibration time on the viability of sperm was not significant different. There was no significant interaction of glicerol and equilibration time on the viability of spermatozoa. In conclution, microencapsulation processes altered the viability of sperm, alginate had an important roles as extracelullar cryoprotectant. Moreover the cryopreservation of microencapsulated sperm might be done using 5 or 7% glycerol in 3-4 hours duration of equilibration time. Key Words: Sperm, Alginate, Microencapsulation, Glycerol, Equilibration Time

Molecular identification technique of Trypanosoma evansi by Multiplex Polymerase Chain Reaction Dyah H. Sawitri; April H. Wardhana; H. Wibowo; M. Sadikin; Fitrine Ekawasti
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Trypanosoma evansi is a Hemoflagella parasite that infects cattle and is known as the agents of Surra. Several other trypanosome species infects mammals: T. equiperdum, T. b. rhodesiense, T. b. gambiense, T. vivax, T. congolense, T.theileri. Some of these species is quite difficult to be distinguished morphologically with T. evansi through conventional techniques (thin blood smear). Molecular technique by polymerase chain reaction (PCR) is reported to have the ability to identify, characterize and diagnose trypanosomes accurately. However, a single PCR used is relatively expensive because it takes at least two or more pairs of primers to determine T. evansi. The purpose of this study is to develop T. evansi species identification techniques by multiplex PCR/mPCR (the three pairs of primer in one reaction) that takes the relatively fast and inexpensive. A total of 31 isolates T.evansi were obtained from Bblitvet Culture Collection (BCC) and the Department of Parasitology BBLitvet used in this study. Isolates represent isolates from endemic areas and Surra outbrake isolated from 1988-2014. DNA extraction performed on each sample, including Bang 87 isolates which has been purified as a positive control. Primers used are specific for T. evansi, the ITS-1, Ro Tat 1.2 VSG and ESAG 6/7. Before running mPCR, each primer is optimized by using a single PCR. The results showed that the three primers can be combined in a single reaction with mPCR technique and amplify each DNA fragment target perfectly, so identified 31 isolates as T. evansi. This technique can be applied in the field with a lower cost and faster time.Key Words: Trypanosoma evansi, Identification, Multiplex PCR

Voice characteristics of some sheep: Utilization to estimation of genetic distance Eko Handiwirawan; Ronny Rachman Noor; Cece Sumantri; . Subandriyo
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Sound analysis has been carried out in various activities including identification and differentiation of species as well as the preparation of the taxonomy of some animals’ species because of several advantages, including no need to capture or too close to the subject observed. Analysis of voice used to differentiate and to estimate of breeds’ sheep genetic distance has not been reported. This research was conducted to study the character of a few breeds’ sheep sound and likely to be used as a predictor of genetic distance between breeds of sheep. The study was conducted in the Animal House at Indonesian Research Institute for Animal Production, Bogor. A total of 20 head adult of five sheeps (St. Croix cross / SC, Barbados Black Belly cross / BC, Local Garut/LG, Composite Garut / KG and Composites Sumatra / KS) used in this study. Call sound recorded using a digital voice recorder. Sound analysis performed by Raven Software Pro 1.3 for Windows to count as many as 24 variables sound. Analysis of variance of each variable sound was performed using PROC GLM of SAS software Ver. 9.0. It used PROC CANDISC for canonical discriminant analysis and then PROC TREE to build a dendogram. The results showed that there were variations in amplitude, energy, power and frequency variables among the five breeds of sheep. By plotting canonical, LG, KS and BC sheep were from a different group. It was concluded that the sound characteristics variables which can be used as a differentiator breeds of sheep were the third quartile frequency, center frequency, maximum frequency and the first quartile time. Dendogram showed that KG sheep was in the less accurate group. Genetic distance estimation method using voice characteristic data may be applied on sheep. Key Words: Characteristics, Call Voice, Differentiation, Genetic Distance, Sheep

Gas production and rumen fermentation characteristics of buffalo diets containing by-product from some sorghum varieties Irawan Sugoro; Komang G. Wiryawan; Dewi Apri Astuti; Teguh Wahyono
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Sorghum is one of potencial fibre sources as buffalo feed. Quality of sorghum could be increased by irradiation mutation breeding. Samurai 1 and Samurai 2 were products of the irradiation mutation breeding of Pahat. This study was conducted to compare buffalo diets containing Samurai 2 sorghum straw and Samurai 1 bagasse sorghum compared with Pahat sorghum straw using in vitro study. Completely randomized design with 6 treatments and 3 replications was applied in this experiment. The treatment diets were P1 (50% Pahat sorghum straw + 50% concentrate), P2 (50% Pahat sorghum straw silage + 50% concentrate), P3 (50% Samurai 2 sorghum straw + 50% concentrate), P4 (50% Samurai 2 sorghum straw silage + 50% concentrate), P5 (50% Samurai 1 sorghum bagasse + 50% concentrate) and P6 (50% Samurai 1 sorghum bagasse silage + 50% concentrate). The 200 mg DM samples of diets were incubated in 30 ml rumen-buffer fluid for 48 hours. Variables measured were total gas production, CH4 production and rumen fermentation characteristics. Results showed that P2 and P4 produce the highest of gas production (P<0.05) with 60.99 and 60.86 ml/200 mg dry matter respectively. Treatments of P1, P2 and P4 produced the lowest CH4 concentration (P<0.05) with 10.57, 10.90 and 9.82% of total gas, respectively. The P4 produced the highest total volatile fatty acids (VFA), dry matter degradability and organic matter degradability with 109.83 mM, 62.93% and 59.97% respectively, meanwhile ammonia (NH3) concentration was not significantly different. The conclusion showed that straw silage of Samurai 2 was comparable to the Pahat sorghum straw and Samurai 1 bagasse sorghum as buffalo diet. Key Words: Buffalo, CH4 Concentration, Rumen Fermentation Characteristics, Sorghum

Role of sweet orange (Citrus sinensis) waste in lowering the meat cholesterol and fat of Padjadjaran sheep Lovita Adriani; Elvia Hernawan; Ujang Hidayat
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This research is aimed to evaluate sweet orange’s role in lowering meat cholesterol and fat in Padjadjaran sheep. Twenty sheeps with body weigth 29.66±2.74 kg and variance coefficient <10% were used in this research using Completely Randomize Design (CRD). The treatments were consists of four level sweet orange addition in ration. The treatments were T1 (0%), T2 (7%), T3 (12%) and T4 (17%) that were repeated 5 times. The research was conducted for five weeks with observed variables are ration consumption that was measured everyday, body gain that was measured every week, cholesterol and triglycerides measured at the end of the research. During the research water consumption was given ad libitum. Meat samples were selected randomly from three for each repeated treatments. Observation result showed the highest decrease on meat cholesterol level was T2 treatment with 9.43±0.04 mg/mg on thigh and 9.71±0.04 mg/mg on breast. Meanwhile for fat, the highest decrease was from T4 treatment with 9.70±3.98 ug/mg dan 10.48±1.85 ug/mg. Rumen’s pH did not show significant difference (P>0.05) on all treatments with value range between 6.80±0.01 - 6.5±0.02. In conclusion, sweet orange (Citrus sinensis) waste is capable to lowering cholesterol level and fat in meat of Padjadjaran sheep. Key Words: Citrus sinensis Waste, Meat Cholesterol , Meat Fat, Padjadjaran Sheep

Carbohydrate and protein digestion on palm kernal cake by Mannanase BS4 and papain cocktail enzymes SIW Rakhmani; Y. Pangestu; Arnold P. Sinurat; Tresnawati Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Supplementation of the mannanase BS4 enzyme on palm kernel cake (PKC) increased its metabolisable energy (ME), and supplementation with protease is expected to increase its protein digestibility. Therefore, the purpose of this research is to determine the optimum proportion of cocktail enzymes between BS4 β-mannanase (produced by Eupenicillium javanicum) and protease (papain) and their degradation activities on carbohydrate and protein of PKC. The β-Mannanase was produced by the mold through solid substrate fermentation (SSF) using coconut meal as the substrate. The papain was extracted from papaya latex (PL), collected by longitudinal incisions on unripe papaya fruit and oven dried overnight. The evaluation of enzyme cocktails for PKC hydrolysis was done at pH 5.8 and 40°C which are similar with poultry intestine condition and both enzymes are still active. The β- mannanase BS4 : papain were mixed with some proportions, i.e.: 100 : 0; 75 : 25; 50 : 50; 25 : 75 and 0 : 100% (by volume) in order to study the optimum cocktail composition ratio. The activities of β-mannanase towards gum locust bean was 86 U.ml-1 and papains PL activity was 18,000 U.g-1respectively. PL was chosen for synergistic reaction and compared with a commercial Merck papain (CMP, 20,000 U.g-1) as positive control. β-Mannanase BS4 showed carbohydrate digestion activity, and protein digestion activity was not detected. Papain showed protein digestion activity and no carbohydrate digestion activity. Enzyme cocktails of 50 : 50 from PL protease showed slightly increased in synergistic protein digestion activity in PKC. However, its reduction sugar production was much lower than 100 : 0 and 75 : 25. Amino acids production by enzyme cocktails 75 : 25 were higher than that of 100 : 0. As a result, the best volume composition of β-mannanase BS4 and papaya latex was 75 : 25 (v:v) or 14 : 10 (U:U).Key Words: Mannanase BS4, Papain, Cocktail-Enzymes, Palm Kernel Cake

Trypanocidals effectivity against some isolates of Trypanosoma evansi propagated in mice Didik Tulus Subekti; I. Yuniarto; . Sulinawati; H. Susiani; F. Amaliah; B. Santosa
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Surra is one of infectious diseases in various types of animals caused by blood parasites called Trypanosoma evansi. It is fatally occurred, especially in horse, buffalo and camel. Surra may be controlled by effectively trypanocidals treatment based on the results of its sensitivity test. Therefore, it is necessary to study the effectiveness of various trypanocidal against some T.evansi isolates originating from several regions in Indonesia with surra case to determine its suitability and efficacy. The test was carried out by pre-test - post-test. Mice were infected by several T.evansi isolates from various infected areas. Their parasitaemia were observed. After reaching peak of parasitemia, the mice were treated by trypanocidal with different doses. Parasitaemia alteration was observed for one month. Observation results showed that all isolates had different sensitivity to the trypanocidals, so that trypanocidals application tended to specific location. Generally, suramin and melarsomine dihydrochloride were the most effective trypanocidal for some Indonesian isolates. In contrast, isometamidium chloride was not recommended to be used for surra control in Indonesia. Key Words: Trypanosoma evansi, Trypanocidal, Parasitemia, Surra

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Ahmadi Riyanto

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Kota bogor,

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Journal Mail Official ahmadi_puslitbangnak@yahoo.com

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

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Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA