Ronny Rachman Noor
Departemen Ilmu Produksi Dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Kampus IPB Darmaga Bogor 16680

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Behaviour characteristics estimation tool of genetic distance between sheep breeds Handiwirawan, Eko; Noor, Ronny Rachman; Sumantri, Cece; ., Subandriyo .
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 4 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1210.684 KB) | DOI: 10.14334/jitv.v19i4.1092

Abstract

Information on the estimation of genetic distances and differentiation among sheep breeds are needed in crossing and conservation programs. This research aims to study of utilizing behaviour characteristic variables to differentiate and estimate genetic distance between the sheep breeds.  The study was conducted at Cilebut and Bogor Animal House of Indonesian Research Institute for Animal Production.  Five sheep breeds used were Barbados Black Belly Cross (BC), Garut Composite (KG), Garut Local (LG), Sumatera Composite (KS) and St. Croix Cross (SC), with total sample of 50 heads.  A total of 10 variables of behavior traits were observed in this study. Analysis of variances and significance tests were applied to compare between sheep breeds and performed for all of behavior traits using PROC GLM of SAS Program ver. 9.0. PROC CANDISC was used for canonical discriminant analyses, the hierarchical clustering was performed using the PROC CLUSTER by Average Linkage method (Unweighted Pair-Group Method Using Arithmetic Averages, UPGMA), and the dendogram for the five sheep breeds was described using PROC TREE.  The differentiator variables for the behavior traits were standing and feeding duration.  The canonical plotting based on behavioral characteristics could differentiate BC, KS and LG (with KG and SC) sheeps. Estimation of genetic distance based on the behavior traits is less accurate for grouping of sheep breeds. Key Words: Behaviour Characteristic, Differentiation, Genetic Distance
Isolation and identification of indigenous lactic acid bacteria from North Sumatra river buffalo milk Rizqiati, Heni; Sumantri, Cece; Noor, Ronny Rachman; Damayanthi, E.; Rianti, E. I.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.693 KB) | DOI: 10.14334/jitv.v20i2.1163

Abstract

Buffalo milk is a source of various lactic acid bacteria (LAB) which is potential as culture starter as well as the probiotic. This study was conducted to isolate and identify LAB from indigenous North Sumatra river buffalo milk. Lactic acid bacteria was isolated and grown in medium De Man Rogosa Sharpe Agar (MRSA). The isolation was conducted to obtain pure isolate. The identification of  LAB was studied in terms of morphology, physiology, biochemistry and survival on low pH. Morphology tests were conducted by Gram staining and cell forming; physiology tests were conducted for growing viability at pH 4.5 and temperature at 45oC; whereas biochemistry tests were conducted for CO2, dextran and NH3 productions. Determination of LAB species was conducted using Analytical Profile Index (API) test CHL 50. Results of identification showed that 41 isolates were identified as LAB with Gram-positive, catalase-negative, rod and round shaped characteristics. Resistance test done to low pH (pH 2) for the lactic acid bacteria showed decrease of bacteria viability up to1.24±0.68 log cfu/ml. The resistant isolates at low pH were L12, L16, L17, L19, L20, M10, P8, S3, S19 and S20. Identification with API test CHL 50 for 10 isolates showed that four isolates were identified as Lactobacillus plantarum, L. brevis, L. pentosus and Lactococuslactis. Key Words: Buffalo Milk, LAB, Isolation, Identification
Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep Harahap, Ratna Sholatia; Noor, Ronny Rachman; Gunawan, Asep
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.88

Abstract

HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fat-tailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.
KURVA PERTUMBUHAN SAPI PERAH FRIES HOLLANDS DARI LAHIR SAMPAI UMUR KAWIN PERTAMA DENGAN MODEL MATEMATIKA LOGISTIC Salman, Lia Budimulyati; Noor, Ronny Rachman; Saefuddin, Asep; Talib, Chalid
Informatika Pertanian Vol 23, No 1 (2014): JULI, 2014
Publisher : Sekretariat Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.706 KB) | DOI: 10.21082/ip.v23n1.2014.p75-84

Abstract

Tujuan utama dalam pembuatan model kurva pertumbuhan adalah untuk deskripsi dan prediksi. Tujuan deskripsi merupakan upaya untuk bisa mempermudah interpretasi dari proses pertumbuhan ternak menjadi hanya beberapa parameter, sedangkan tujuan prediksi lebih fokus bagaimana metode untuk memprediksi dari beberapa parameter. Adapun tujuan penelitian ini adalah untuk membuat model kurva pertumbuhan sapi perah Fries Hollands dari lahir sampai siap kawin yang sesuai dengan situasi dan kondisi skala industri peternakan sapi perah. Data bobot badan sapi betina yang digunakan dalam penelitian ini merupakan hasil penimbangan bobot badan dari sapi perah Fries Hollands yang dipelihara oleh PT Taurus Dairy Farm Sukabumi dari tahun 2001 sampai 2011, sejumlah 1221 ekor, individu yang mempunyai data lengkap dari lahir sampai umur kawin pertama sebanyak 373 ekor. Sedangkan untuk data BPPTU Baturraden 214 ekor data kelahiran sampai ternak siap untuk dikawinkan pertama kali dari 2010 sampai 2011. Data dianalisis menggunakan program SAS 9.2 dengan prosedur NLIN (Non Linear). Kurva yang dihasilkan dapat dipakai sebagai standar kurva pertumbuhan sapi perah Fries Hollands di Indonesia dari lahir hingga siap kawin. Model matematik Logistic dapat dipakai untuk menduga kurva pertumbuhan karena mempunyai tingkat akurasi yang tinggi dengan nilai koefisien determinasi (R2) lebih dari 90%.
Analisis Keterkaitan GEN BMP15, BMPR1B, dan KISS1 dengan Sifat Fekunditas pada Kambing Peranakan Etawah Betina Rini Herlina Mulyono; Cece Sumantri; Ronny Rachman Noor; . Jakaria; Dewi Apri Astuti
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 2 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.746 KB) | DOI: 10.18343/jipi.24.2.83

Abstract

Etawah grade goat is a result of grading up line between Kacang and Etawah goats, which well adapted to Indonesia’s humid tropical climate. This goat is a dual-purpose goat breed for milk and meat production. The objectives of this study were to evaluate the polymorphism of fecundity genes through the investigation of four candidate genes for prolificacy, i.e., BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) and their associations with the litter size of Etawah-Grade does. The diversity of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (introns 1) genes were analyzed by the PCR-RFLP technique. A total of 106 of DNA samples were collected from Etawah-Grade does at BPTU-HPT Pelaihari in Pelaihari, South Kalimantan (51 heads) and Cordero Farm in Bogor, West Java (55 heads). Genetic polymorphisms between the two Etawah-Grade groups were calculated as well as their allele and genotype frequencies and the Polymorphic Informative Content (PIC). The Kruskal-Wallis one-factor ranked analysis of variance was used to determine whether there was any effect of genotype of the fecundity genes on litter size. The polymorphic information content (PIC) estimated at BPTU-HPT Pelaihari was 0.313, whereas at Cordero farm was 0.174, and overall PIC was 0.244. There was no association between genotypes of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) genes and litter size. Results indicated that BMP15 (exon 1) and KISS1 (exon 1) genes were monomorphic. However, the other two loci showed polymorphisms. The BMPR1B (exon 1) locus had two genotypes with the frequency for CC (96.23%) and for GG (3.77%), and KISS1 (intron 1) locus had three genotypes with the frequency for TT (80.19%), AT (17.92%), and AA (1.89%).
Behaviour characteristics estimation tool of genetic distance between sheep breeds Eko Handiwirawan; Ronny Rachman Noor; Cece Sumantri; Subandriyo . .
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 4 (2014): DECEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1210.684 KB) | DOI: 10.14334/jitv.v19i4.1092

Abstract

Information on the estimation of genetic distances and differentiation among sheep breeds are needed in crossing and conservation programs. This research aims to study of utilizing behaviour characteristic variables to differentiate and estimate genetic distance between the sheep breeds.  The study was conducted at Cilebut and Bogor Animal House of Indonesian Research Institute for Animal Production.  Five sheep breeds used were Barbados Black Belly Cross (BC), Garut Composite (KG), Garut Local (LG), Sumatera Composite (KS) and St. Croix Cross (SC), with total sample of 50 heads.  A total of 10 variables of behavior traits were observed in this study. Analysis of variances and significance tests were applied to compare between sheep breeds and performed for all of behavior traits using PROC GLM of SAS Program ver. 9.0. PROC CANDISC was used for canonical discriminant analyses, the hierarchical clustering was performed using the PROC CLUSTER by Average Linkage method (Unweighted Pair-Group Method Using Arithmetic Averages, UPGMA), and the dendogram for the five sheep breeds was described using PROC TREE.  The differentiator variables for the behavior traits were standing and feeding duration.  The canonical plotting based on behavioral characteristics could differentiate BC, KS and LG (with KG and SC) sheeps. Estimation of genetic distance based on the behavior traits is less accurate for grouping of sheep breeds.
Voice characteristics of some sheep: Utilization to estimation of genetic distance Eko Handiwirawan; Ronny Rachman Noor; Cece Sumantri; . Subandriyo
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.751 KB) | DOI: 10.14334/jitv.v20i4.1274

Abstract

Sound analysis has been carried out in various activities including identification and differentiation of species as well as the preparation of the taxonomy of some animals’ species because of several advantages, including no need to capture or too close to the subject observed. Analysis of voice used to differentiate and to estimate of breeds’ sheep genetic distance has not been reported. This research was conducted to study the character of a few breeds’ sheep sound and likely to be used as a predictor of genetic distance between breeds of sheep. The study was conducted in the Animal House at Indonesian Research Institute for Animal Production, Bogor.  A total of 20 head adult of five sheeps (St. Croix cross / SC, Barbados Black Belly cross / BC, Local Garut/LG, Composite Garut / KG and Composites Sumatra / KS) used in this study. Call sound recorded using a digital voice recorder. Sound analysis performed by Raven Software Pro 1.3 for Windows to count as many as 24 variables sound. Analysis of variance of each variable sound was performed using PROC GLM of SAS software Ver. 9.0. It used PROC CANDISC for canonical discriminant analysis and then PROC TREE to build a dendogram. The results showed that there were variations in amplitude, energy, power and frequency variables among the five breeds of sheep. By plotting canonical, LG, KS and BC sheep were from a different group. It was concluded that the sound characteristics variables which can be used as a differentiator breeds of sheep were the third quartile frequency, center frequency, maximum frequency and the first quartile time. Dendogram showed that KG sheep was in the less accurate group. Genetic distance estimation method using voice characteristic data may be applied on sheep. Key Words: Characteristics, Call Voice, Differentiation, Genetic Distance, Sheep
Fluctuating Asymmetry Increases with Heat Stress Disruptions on Bali Cattle (Bos javanicus) at Different Altitude Ikhsan Suhendro; Jakaria Jakaria; Rudy Priyanto; Wasmen Manalu; Ronny Rachman Noor
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2758

Abstract

Bali cattle (Bos javanicus) are common species distributed throughout Indonesia to survive in tropical hot, and humid climates. Fluctuating asymmetry (FA) is a random deviation from perfect bilateral symmetry. FA is often used to measure developmental stability in individuals and can be used as a marker for the impact of environmental and genetic stress. This study aims to investigate the pattern of developmental instability in Bali cattle as caused by environmental stress at various altitudes using the FA index. FA indexes in this study were used to compare asymmetrical physical traits of Bali cattle in Sembalun high altitude and Serading low altitude. Sixty-five Bali cattle were used in this study reared at different altitudes, namely Serading, Sumbawa Island (50 m above sea level), and Sembalun, Lombok Island (1,186 m above sea level). The physical traits of Bali cattle measured were horn circumference (horn), a distance of hip to spine bone (pelvic), metatarsal circumference (metatarsal), and metacarpal circumference (metacarpal). The FA1 and FA5 indexes showed significant metacarpal differences between Bali cattle reared at Sembalun and Serading population (P<0.05). FA11 multiple trait index as a composite of all traits revealed a significant difference as well. (P<001). It can be concluded that various heat stress by altitude impacts the asymmetry of Bali cattle.
Isolation and identification of indigenous lactic acid bacteria from North Sumatra river buffalo milk Heni Rizqiati; Cece Sumantri; Ronny Rachman Noor; E. Damayanthi; E. I. Rianti
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.693 KB) | DOI: 10.14334/jitv.v20i2.1163

Abstract

Buffalo milk is a source of various lactic acid bacteria (LAB) which is potential as culture starter as well as the probiotic. This study was conducted to isolate and identify LAB from indigenous North Sumatra river buffalo milk. Lactic acid bacteria was isolated and grown in medium De Man Rogosa Sharpe Agar (MRSA). The isolation was conducted to obtain pure isolate. The identification of  LAB was studied in terms of morphology, physiology, biochemistry and survival on low pH. Morphology tests were conducted by Gram staining and cell forming; physiology tests were conducted for growing viability at pH 4.5 and temperature at 45oC; whereas biochemistry tests were conducted for CO2, dextran and NH3 productions. Determination of LAB species was conducted using Analytical Profile Index (API) test CHL 50. Results of identification showed that 41 isolates were identified as LAB with Gram-positive, catalase-negative, rod and round shaped characteristics. Resistance test done to low pH (pH 2) for the lactic acid bacteria showed decrease of bacteria viability up to1.24±0.68 log cfu/ml. The resistant isolates at low pH were L12, L16, L17, L19, L20, M10, P8, S3, S19 and S20. Identification with API test CHL 50 for 10 isolates showed that four isolates were identified as Lactobacillus plantarum, L. brevis, L. pentosus and Lactococuslactis.
Performance of the third generation striped catfish, Pangasianodon hypophthalmus Sauvage, 1878 as results of the selection for bodyweight character in Freshwater Aquaculture Fisheries Center, Sungai Gelam, Jambi Irwan Irwan; Dinar Tri Soelistyowati; Odang Carman; Ronny Rachman Noor
Jurnal Iktiologi Indonesia Vol 19 No 3 (2019): October 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i3.469

Abstract

Selective breeding of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) has been conducted at BPBAT Sungai Gelam to produce a growth line with mass selection method. Until 2018, the selective breeding program has produced three generations. Therefore, it was necessary to evaluate the performance of the third generation growth line (G3Ps) at the reproductive, seed and grow-out phase compared to the second generation the base population (G2Ds). A total of 10 pairs of broodstock (G3Ps and G2Ds) were spawned then the seeds were raised for 120 days in the media with regular water exchange (first treatment: T1) and without water exchange (second treatment: T2). The results showed that the number of eggs per gram of G3Ps (1600±124 eggs g-1) was significantly different from G2Ds (1490±101 eggs g-1). The bodyweight of G3Ps seeds at aged 40 days larger than G2Ds with selection response is 32.25%, while survival and feed efficiency were not significantly different. At the grow-out phase, between treat-ments were not significantly different and there was no interaction between lines and treatments for all the characters measured (p>0.05). The bodyweight of G3Ps was larger than the G2Ds with response selection 18.41% in T1 and 42.6% in T2. The control used was the base population so that the selection response obtained was an accumulation of three generations. Thus the selection response per generation was 6.14% in T1 and 14.20% in T2 measured at 162 days from hatching. It can be concluded that there is an improvement in the character of bodyweight for the third generation of growth line (G3Ps) as results of the selection at BPBAT Sungai Gelam both in good (T1) and bad environment (T2). Abstrak Seleksi ikan patin siam (Pangasianodon hypophthalmus Sauvage, 1878) telah dilakukan di BPBAT Sungai Gelam untuk meningkatkan pertumbuhan dengan metode seleksi individu. Sampai tahun 2018, seleksi tersebut telah meng-hasilkan tiga generasi. Penelitian ini bertujuan untuk mengevaluasi performa generasi ketiga galur pertumbuhan (G3Ps) pada tahap reproduksi, pertumbuhan benih, dan ukuran konsumsi dibandingkan dengan populasi dasar gene-rasi kedua (G2Ds). Sebanyak 10 pasang induk G3Ps dan G2Ds dipijahkan kemudian benih yang dihasilkan dibesarkan selama 120 hari pada media dengan pergantian air secara berkala (perlakuan pertama: T1) dan tanpa pergantian air (perlakuan kedua: T2). Hasil penelitian menunjukkan bahwa jumlah telur per gram adalah 1600±124 butir pada G3Ps, berbeda nyata dengan G2Ds yaitu 1490±101 butir. Benih G3Ps umur 40 hari memiliki bobot tubuh lebih besar diban-dingkan G2Ds dengan respons seleksi sebesar 32,25%, sedangkan sintasan dan efisiensi pakan tidak berbeda nyata. Pada tahap pembesaran ukuran konsumsi, antar perlakuan tidak berbeda nyata dan tidak ada interaksi antargalur dan perlakuan untuk karakter bobot tubuh, panjang baku, sintasan dan efisiensi pakan (p>0,05). Karakter bobot tubuh G3Ps lebih besar dibandingkan dengan G2Ds dengan respons seleksi total untuk tiga generasi sebesar 18,41% pada T1 dan 42,6% pada T2. Dengan demikian respons seleksi per generasi sebesar 6,14% pada T1 dan 14,20% pada T2 yang diukur pada umur 162 hari dari menetas. Disimpulkan bahwa terjadi perbaikan pada karakter bobot tubuh untuk galur pertumbuhan generasi ketiga (G3Ps) hasil program seleksi di BPBAT Sungai Gelam baik pada lingkungan baik (T1) maupun lingkungan buruk (T2).