cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 7, No 1 (2021): April 2021" : 8 Documents clear
Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies Muhammad Nur `Adam Hatta; Ezanee Azlina Mohamad Hanif; Siok-Fong Chin; Hui-min Neoh
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.10129

Abstract

Colon cancer-associated gut bacteria were mostly identified via next-generation sequencing in gut microbiome profiling studies. Anaerobic culture systems can be used to culture colon cells with these gut bacteria to further confirm the tumorigenic properties of these bacteria. Nevertheless, it is unclear how colon cells will grow in an anaerobic environment, as most cells are cultured aerobically. Therefore, we investigated the survival and viability of HT-29, a colon cancer cell line in an anaerobic culture system, and compared it to the usual culture condition in an aerobic setup. Interestingly, we found that HT-29 was able to grow in the anaerobic setup. Its viability was similar for both culture conditions, with only a slower growth rate observed in the anaerobic setup. Furthermore, gene expression studies showed that the cells were not under severe anaerobic stress even when exposed to the oxygen-deprived environment.This study provided results on some baseline parameters of an anaerobic colon cell culture system, and will be useful for journal readers who wish to investigate functional properties of anaerobic bacteria.
Fragmentomic cfDNA Patterns in Noninvasive Prenatal Testing and Beyond Kavish Kohabir; Rob Wolthuis; Erik A. Sistermans
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.10229

Abstract

The release of fetoplacental cell-free DNA (cfDNA) into the maternal bloodstream opened up new avenues towards noninvasive prenatal testing (NIPT) for aneuploidies, hereditary DNA mutations and other pregnancy-related developmental disorders. Increasingly, cfDNA catches interest for its noninvasive screening value in other areas as well, including oncology. Although there are indications that cfDNA fragmentation is a non-random process, the etiology and different structural aspects of cfDNA are still not well known. The emerging field of cfDNA fragmentomics investigates the existence of tissue and disease specific cfDNA signatures and the chemistry and biology underlying the fragmentation process. This review sheds light on recent developments in cfDNA fragmentomics and illustrates their significance in NIPT improvement and beyond.We discuss aspects of fragment size distributions, epigenetic correlations and putatively enriched cfDNA fragment-end compositions. Combinatorial fragmentomic efforts have provided more insights into the roles of different enzymes that contribute to the fragmentation process in the tissue of origin and in the bloodstream. Altogether, these studies revealed multiple fragmentomic-related biomarkers that can be used to make noninvasive screening and other types of clinical use of cfDNA more robust, by raising its distinctive capacities. This includes multiple complementary approaches to determine the fetal fraction, a key determinant in NIPT. Furthermore, these developments translate to a better understanding of the encountered cfDNA patterns and will catalyze the expansion of screening possibilities in NIPT and beyond
Effect of Aerobic Exercise on Left Ventricular Connexin43 Expression and Distribution in Juvenile and Young Adult Rats Rustiana Tasya Ariningpraja; ‪Dewi Irawati Soeria Santoso; Ahmad Aulia Jusuf; Alfrina Hany
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.10690

Abstract

Background: Gap Junction (GJ) plays a role in supporting the heart electricity. Connexin43 (Cx43) as the main protein constituent of GJ in left cardiac ventricle, will increase in number and slightly redistributed to the lateral sides of cardiomyocytes after aerobic exercise in adulthood. The effects of aerobic exercise that begin at childhood are not well known.Objective: This study aims to observe the effect of aerobic exercise which started from childhood on left ventricle Cx43 distribution.Methods: This study was conducted on 28 male Juvenile (4 weeks) and young adult (8 weeks) rats, divided into 7 groups: 1) Juvenile rats undergoing 4 weeks of exercise (E-J4); 2) Control E-J4 (C-J4); 3) Juvenile rats undergoing 8 weeks of exercise (E-J8); 4) Control EJ-8 (C-J8); 5) Juvenile rats undergoing 12 weeks of exercise (E-J12; 6); Young adult rats undergoing 8 weeks of exercise (E-Yo8); 7) Control E-J12 and E-Yo8 (C-JY128). Exercise group will undergo different length of duration, starting from week 4 until 12 weeks. Cx43 was identified by immunohistochemical staining and analyzed with ImageJ software. Comparison was analyzed using independent t-test.Results: Insignificant lower of total Cx43 expression in E-J4 (64200.45 + 4243.676 total area, p >0.05) compared to control. In contrast, a significant higher of total Cx43 expression was observed in EJ-8, EJ-12 and E-Yo8 (80152.95 + 3760.481, p = 0.001; 75596.775 + 3976.333, p = 0,002; 81216.85+ 2475.768, p = 0,000). Slightly higher of lateral Cx43 redistribution occurred in all aerobic exercise, with significant lateralization in E-J8 and E-Yo8.Conclusion: Aerobic exercise increases Cx43 and slightly redistributed to lateral myocytes under normal condition both in juvenile and young adult rats.
Cushing’s syndrome manifesting as chronic insomnia caused by adrenal cortical adenoma with incidental pituitary microadenoma: a case report Rakhmayanti, Rizky; Minuljo, Tania Tedjo; Suryawati, Herlina; Fitrikasari, Alifiati; Soedarso, Mohamad Adi; Priambada, Dody
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.9247

Abstract

Background: Cushing’s syndrome is condition caused by excessive glucocorticoid with insomnia as one of its neuropsychiatric manifestation. Cushing’s syndrome may be caused by excessive adrenocorticotropin hormone (ACTH-dependent), for example from ACTH producing pituitary tumors, or by overproduction of cortisol by adrenocortical tumors. In this report, we presented a case with Cushing’s syndrome manifesting as chronic insomnia with adrenal cortical adenoma and pituitary microadenoma.Case presentation: A 30-year-old woman was consulted from the Neurologic Department to the Internal Medicine Department with the chief complaint of insomnia and worsening headache since 6 months prior to the admission. She had undergone head MRI and abdominal CT scan previously and was found to have both pituitary microadenoma and left adrenal mass. From the physical examination she had clinical signs of Cushing’s syndrome like Cushingoid face and purplish striae on her stomach. Midnight cortisol serum examination was done initially and showed high level of cortisol. High dose dexamethasone suppression test or DST (8 mg overnight) was later performed to help determine the main cause of Cushing’s syndrome. The result failed to reach 50% suppression of cortisol serum, suggestive that the Cushing’s syndrome was not ACTH-dependent from the pituitary but potentially from overproduction of cortisol by the left adrenal mass. Therefore, left adrenalectomy was performed and the histopathological study supported the diagnosis of adrenal cortical adenoma.Conclusion: Chronic insomnia is a very important symptoms of Cushing’s syndrome that should not be neglected. The patient had both microadenoma pituitary and left adrenal mass thus high dose DST test (8 mg overnight) needed to be performed to differentiate the source of Cushing’s syndrome. The result showed only little suppression therefore the pituitary microadenoma was not the source of Cushing’s syndrome and more suggestive from the adrenal etiology.
B- Acute Lymphoblastic Leukemia L2 In Second Trimester of Pregnancy Iraisa rosaria; Dwi Retnoningrum
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.9542

Abstract

Background: Acute lymphoblastic leukemia (ALL) in adults tends to have a poor prognosis and even more challenging to treat during pregnancy due to the mother and the fetus’s safety issue. Despite commonly found in 2nd and 3rd trimester, ALL found during 2nd trimester needs more comprehensive management on maintaining the pregnancy while chemotherapy cannot be delayed.Case Presentation: A 36-year-old woman at 27 weeks of gestation visited the hospital with multiple cervical lymphadenopathy and major weight loss for the last six months. Bicytopenia with leukocytosis is found, along with an increase in LDH, Ferritin, and low albumin level. Bone marrow biopsy had confirmed the diagnosis of ALL-L2. Positive immunophenotyping results on HLA-DR, CD10, CD19, CD20, which support the lymphoid Line-B subtype. The patient was treated with Vincristine 2 mg/IV weekly and 100 mg of oral prednisone for six weeks and maintain the pregnancy. Successful delivery was carried out at 32 weeks of gestational age by lower segment cesarean section due to premature rupture of the membrane. A baby girl was born weighed 1700 gram, APGAR Score 8/9/9, and has no disability on clinical or hematological features at the moment.Conclusion: ALL in pregnancy is very rare and extremely aggressive disease unless promptly treated. In this case report, it was a first pregnancy in advanced maternal age mother with high social value baby and can be treated succesfully using single regimen of chemoteraphy during pregnancy even though at the first time administered to hospital the mother come with critical clinical presentation. Leukemia in pregnancy is challenging and still need further study to increase the safety and better treatment outcome.Keywords: ALL in pregnancy, ALL-L2, Line-B Lymphoid, Immunophenotyping ALL
Prevalence and Risk Factors of ESBL-producing Enterobacteriaceae in The Community Nia Krisniawati; Anriani Puspita Karunia Ning Widhi
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.10051

Abstract

Background: World Health Organization (WHO) data collection of Escherichia coli resistant to cephalosporin generation III already confirmed in 86 countries. Incredibly high carrier prevalence rates were also widely developed in Thailand, Egypt, and China. The Faculty of Medicine's research team at Jenderal Soedirman University, Purwokerto,  discovered at the beginning of 2018 that Extended Spectrum β-Lactamase (ESBL) E. coli carriers throughout the 2015 class students were 26.8 percent.Objective: This research investigated the Prevalence and associated factors of ESBL-producing Enterobacteriaceae (EPE) asymptomatic carriers in the community.Methods: The participant fill a questionnaire, and samples were taken from rectal swabs using Amies transport medium (Labware Charuzu), and then models were analyze using HiCrome ™ ESBL Agar Base (Himedia, India). Analysis of its Prevalence and Resistance Predictors uses IBM SPSS Statistics Version 22.0 for Windows (Armonk, NY: IBM Corp).Results: The Prevalence of EPE asymptomatic carriers in the community in Purwokerto was 66.7%. In the bivariate analysis, subjects who took antacids in the last eight weeks, history of hospitalization in the previous 12 months, the habit of consuming milk, yogurt, cheese, meat, seafood, and raw vegetables did not show any significant difference. Frequent chicken and freshwater fish consumption tended to be a risk factor for ESBL-producing Enterobacteriaceae with PR 1.462, 95% CI (1.115-1.918); PR 1.666, 95% CI  (0.936-2.966); however, in the multivariate logistic regression analysis, this was not significant.Conclusion: The Prevalence of EPE asymptomatic carriers in the community in Purwokerto is 66.7%. All variables did not become any significant of ESBL-producing Enterobacteriaceae. However, ESBL remains an emerging antimicrobial resistance.
Degree of coronary occlusions links to the patient clinical outcome: four cases of double culprits acute ST-segment elevation myocardial infarction Ilham Uddin; Anindia Wardhani; Misbah Hari Cahyadi; Desy Ayu Puspitasari; Pipin Ardhianto; Udin Bahrudin; Yan Herry; Sodiqur Rifqi
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.10693

Abstract

Background: Double coronary culprit lesions in ST-segment elevation myocardial infarction (STEMI) is uncommon. Despite successful primary percutaneous coronary intervention (PPCI) in all culprit lesions, the clinical outcome remains unfavorable and the possible factors for the outcome are not fully understood.Cases Presentation: We reported four cases of double culprit lesions STEMI underwent PPCI. Patient A, a 62 y.o. male with extensive anterior-inferior STEMI, had total occlusion (TO) at both proximal left anterior descending (LAD) and mid right coronary artery (RCA). Patient B, a 42 y.o. male with extensive anterior-inferior STEMI, had subtotal occlusion (STO) at proximal RCA and TO at proximal LAD. Both of them had RBBB ECG pattern. Patient C, a 67 y.o. male with inferior STEMI had 90% thrombus occlusion at proximal–mid LAD and TO at proximal RCA. Patient D, a 65 y.o. male with anteroseptal STEMI, had STO at proximal LAD and 80% thrombus occlusion at mid left circumflex. The cardiomyocyte infarction biomarkers increased in all patients. Although all of them underwent successful PPCI in all of culprit lesions, they suffered from acute heart failure and two of them experienced recurrent ventricular arrhythmia episodes. One of them (patient A) died two days post PPCI. He was only patient who suffering from total occlusion in LAD and RCA with TIMI thrombus 5 and experienced a total atrioventricular block post-PPCI.Conclusion: STEMI with coronary double culprits have severe clinical outcome, regardless of the successful PCI. The degree of coronary occlusions might be linked to the patient clinical outcome. 
The Evaluation of Parental Acceptance Towards Children with Sex Chromosomal Disorders of Sex Development Using A Mixed-Method Iit Fitrianingrum; Annastasia Ediati; Tri Indah Winarni; Sultana MH Faradz
Journal of Biomedicine and Translational Research Vol 7, No 1 (2021): April 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i1.10710

Abstract

Background: Sex chromosomal Disorder of sex development (DSD) is an atypical abnormality of external genitalia which is mismatched with its sex chromosome traits. The condition of children with DSD affects the dynamics in the family. Parents’ reactions after discovering this health problem vary greatly, such as being in a state of shock, confusion, or self-blame. However, parents’ acceptance is extremely important for better quality of caring, to the healthy social and emotional child development, and to make the best decisions regarding gender assignment.Objective: To describe the acceptance process of parents that have children with sex chromosomes mosaicism DSD.Methods: This study used a mixed-method with a sequential explanatory approach, which was preceded by quantitative data collection followed by qualitative. The total respondents consisted of 14 mothers and 12 fathers of 14 sex chromosome mosaicism DSD patients with XX/XY, X/XY, XYY or XXY variants. Quantitative data were collected using the Indonesian version of the Parental Acceptance-Rejection Questionnaire (PARQ), and interviews were conducted to determine the acceptance process.Results: Most acceptance cases were based on the surgical stage completion in which a higher number of mothers (71.43%) than fathers (50%).Conclusion: It is uneasy for parents to accept children with sex chromosome mosaicisms DSD, hence the fathers struggle more than mothers in accepting those affected. To the best of our knowledge this is the first study in Indonesia to help parent understand and accept their child condition.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2021 2021


Reset
Filter By Issues
All Issue Vol 11, No 3 (2025): December 2025 Vol 11, No 2 (2025): August 2025 Vol 11, No 1 (2025): April 2025 Vol 10, No 3 (2024): December 2024 Vol 10, No 2 (2024): August 2024 Vol 10, No 1 (2024): April 2024 Vol 9, No 3 (2023): December 2023 Vol 9, No 2 (2023): August 2023 Vol 9, No 1 (2023): April 2023 Vol 8, No 3 (2022): December 2022 Vol 8, No 2 (2022): August 2022 Vol 8, No 1 (2022): April 2022 Vol 7, No 3 (2021): December 2021 Vol 7, No 2 (2021): August 2021 Vol 7, No 1 (2021): April 2021 Vol 6, No 3 (2020): December2020 Vol 6, No 2 (2020): Augusts 2020 Vol 6, No 1 (2020): April 2020 Vol 5, No 2 (2019): December 2019 Vol 5, No 1 (2019): July 2019 Vol 4, No 2 (2018): December 2018 Vol 4, No 1 (2018): July 2018 Vol 3, No 2 (2017): December 2017 Vol 3, No 1 (2017): July 2017 Vol 2, No 2 (2016): December 2016 Vol 2, No 1 (2016): July 2016 Vol 1, No 2 (2015): December 2015 Vol 1, No 1 (2015): April 2015 More Issue