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Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 8, No 2 (2022): August 2022" : 8 Documents clear
The Effects of Exercise on Spleen Fibrosis and Macrophage Number in D-Galactose-Induced Aging Rat Model Pramita Dwinda Mulyana; Widya Wasityastuti; Fajar Dwi Astarini; Nungki Anggorowati; Nur Arfian
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14199

Abstract

Background: Spleen plays a role in human immune system as well as in recycling and filtering blood. The aging spleen is associated with fibrosis and impaired immune system, increasing individual vulnerability to getting infections. Regular physical activity is essential to maintain and enhance body fitness, endurance, and immune system.Objective: This study aimed to investigate the effects of mild and moderate-intensity treadmill exercise on the aging spleen by examining fibrosis and the number of macrophages in d-Galactose-induced aging rat models.Methods: Twenty-four male Wistar rats were randomly divided into four groups: G1 (control group), G2 (d‐Galactose, no exercise), G3 (d‐Galactose, low-intensity exercise), and G4 (d‐Galactose, moderate-intensity exercise). d-Galactose was administered intraperitoneally on day 0 and treadmill exercise was given for four weeks following the modified Brown et al. (2007) protocol. Spleens were histologically processed and stained for picrosirius red and against CD68+ antibody. Percentage of fibrosis fraction area and macrophage cell count were obtained using ImageJ, and the data were analyzed statistically using SPSS software.Results: The aging spleen did not show any differences in weight, length, and width among groups (P > 0.05). The administration of d-Galactose in rats causes fibrosis and an increased number of macrophages. Low and moderate-intensity treadmill exercise could not lower the percentages of fibrosis fraction area. However, the moderate-intensity treadmill exercise was effective in lowering macrophage number.Conclusion: Moderate-intensity treadmill exercise was effective in lowering macrophage cell count in the aging rat spleen induced by d-Galactose.
The Effect of Tempeh gembus on Malondialdehyde and Superoxide Dismutase Enzyme Levels in Rats with Diet-Induced Metabolic Syndrome Pristina Adi Rachmawati; Diana Nur Afifah; Ninik Rustanti; Gemala Anjani; Ahmad Zulfa Juniarto
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14427

Abstract

Background: High level of malondialdehyde (MDA) and low level of superoxide dismutase (SOD) are markers of oxidative stress and indicate enhance metabolic syndrome. Tempeh gembus is a food that has antioxidant properties which can decreased MDA concentration and increased SOD enzyme activity.Objective: The aim of this research was to determine the effect of Tempeh gembus on plasma MDA and serum SOD enzyme levels in metabolic syndrome rats.Methods: A post-test only experimental design was used in which 25 Sprague Dawley rats were divided into 5 equal groups of 2 control groups (K- and K+) and 3 treatment groups (P1, P2, P3). Metabolic syndrome was induced in the K+ control and the 3 treatment groups. Tempeh gembus were given for 4 weeks with the doses of 2.5g (P1), 5g (P2), and 7.5g (P3). Plasma MDA levels were measured by the Thiobarbituric Acid Reactive Substance (TBARS) method and serum SOD enzyme levels were measured by the Enzyme Linked Immunosorbent Assay (ELISA) method. The statistical analysis used One Way Anova test.Results: Tempeh gembus significantly decreased plasma MDA levels of metabolic syndrome rats (P=0.000) but had no significant effect on serum SOD enzyme levels in each treatment group (P=0.449).Conclusion: The dose of 7.5g Tempeh gembus was the most effective dose in reducing the plasma MDA level. Tempeh gembus in the 3 treatment doses significantly decreased plasma MDA levels but had no significant effect on serum SOD enzyme levels.
Elevated levels of serum glucose, triglyceride, and liver enzymes in a rat model of 7,12 dimethylbenz[a]anthracene (DMBA)-induced carcinogenesis Ika Yustisia; Padlianah Padlianah; Mutmainah Arif; M. Husni Cangara
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14268

Abstract

ABSTRACT Background: Uncontrolled cell proliferation in cancers has a high requirement of energy and biosynthetic substrates. Glucose and triglycerides are the main source of energy as well as the primary building blocks for forming cellular components of mammalian cells.Objective: This study aims to evaluate the shifting of serum glucose, triglyceride, and nitrogen wastes in the form of urea and creatinine levels; and liver enzymes levels, alanine transaminase (ALT) and aspartate aminotransferase (AST), in rat model carcinogenesis with a single dose of 7,12-dimethylbenzanthracene (DMBA) for 16 weeks observation..Methods: The experimental animals of Rattus norvegicus strain Sprague Dawley were divided into two groups, namely the control group and the DMBA-induced group. A blood chemistry examination was carried out at weeks 4, 8, 12, and 16 post-induction using a spectrophotometer. In addition, observation of breast tumor formation and histological examination of the tumor and organs, including liver, lung, intestine, and kidney, were performed to confirm cancer formation.Results: Five of the six experimental rats (83.3%) induced by DMBA experienced breast and lung cancer formation accompanied by continuous weight loss starting at week 10 after induction. Serum glucose levels increased significantly at weeks 12 and 16 after induction, while serum triglyceride, ALT, and AST levels increased significantly from week 4 after induction until the end of the experiment. Serum urea levels did not show a significant difference from the control group. Nonetheless, creatinine decreased at the last examination.Conclusion: Elevated serum glucose, triglycerides, ALT, and AST levels escorted the chemical carcinogen-induced cancer development. Studies at the clinical level are needed to prove whether abnormally elevated of these blood chemistry levels can be used to detect the presence of cancer early.Keywords:DMBA, breast cancer, lung cancer, cancer metabolism.
Comparison of Testosterone Undecanoate (TU) and Testosterone Enanthate (TE) with Combination of Depot Medroxyprogesterone Acetate (DMPA) on Spermatogenic Cells in Men Retnaningtyas Siska Dianty; Yurnadi Hanafi Midoen
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.15318

Abstract

Introduction: Male hormonal contraception known as safe, effective, and reversiblecontraception. World Health Organization (WHO) multicentre study has been conducted aclinical study administrating of testosterone regimens and progestin to male with a remarkableresult. Azoospermia or severe oligozoospermia occurred in men with injection of TestosteroneEnanthate (TE) and Depot Medroxyprogesterone Acetate (DMPA) as well as TestosteroneUndecanoate (TU) and DMPA for a longer period. Decreasing gonadotropin as well astestosterone could lead to the amount of spermatogenic cells population declining by suppressingthe development of many kinds of cells. This narrative review was to compare howspermatogenic suppression in TE and TU with Combination of DMPA. Method: We arerandomly select article or journal from several databases and individual journal in certainkeywords. The keyword used were : (male hormonal contraception) OR (regimen testosterone))AND (progestin) OR (DMPA) OR (Testosterone enanthate) OR (Testosterone undecanoate) OR(male hormonal contraception combination)) AND (DMPA to spermatogenic cells) OR(Testosterone and DMPA to germ cell development)). After initial searching from databases andindividual journal website, 16 scientific articles or journals selected for the review. Result: Theresult indicated that injection of TE and DMPA had suppressed spermatogenesis, might notsuppressed sperm production for a longer period but the decreasing in type B spermatogoniauntil pachytene spermatocyte did occur. On the other hand, injection of TU and DMPA couldmaintain the suppression of sperm production for a longer period due to sustainable higher serumMPA with combination to even longer-acting TU would have more profound effect for that.Conclusion: It was known that combination of TU and DMPA might have better suppression onsperm production than TE and DMPA, otherwise further research needs to be obtained from thecombination of TU and DMPA on spermatogenic development and the association withintratesticular testosterone.
The Effectiveness of Ethanol Extract of Neem Leaf (Azadirachta indica) Mouthwash Against The Growth of Streptococcus sp. Muhammad Abdurrahman; Endang Sri Lestari; Tyas Prihatiningsih
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14732

Abstract

Background: Streptococcus sp. is a bacteria group in the oral cavity that plays a significant role in initiating plaque biofilm formation on teeth. One way to control plaque is gargling with mouthwash. Neem leaves contain antibacterial compounds such as alkaloids, steroids, glycosides, and flavonoids that can be formulated as mouthwash ingredients.Objective: The study aimed to determine the effectiveness of Ethanol Extract of Neem Leaf (EENL) mouthwash on the growth of Streptococcus sp.Methods: The research design was a pretest-posttest control group. The 28 samples of Streptococcus sp. were divided into 7 treatment groups (4 repetitions). The treatment group was given EENL mouthwash with concentrations of 2.5%, 5%, 7.5%, 10%, and 12.5%, a negative control was given aquadest and positive control was given 0.2% chlorhexidine gluconate. Bacterial growth is known through the absorbance values. Data analysis used one-way Anova test and post hoc LSD test at p<0.05Results: EENL mouthwash has antibacterial activity against Streptococcus sp. One way ANOVA test on the difference in absorbance values shows a p=0.00. LSD post hoc test between EENL concentration of 2.5% and chlorhexidine 0.2% showed equivalent antibacterial activity (p>0.05). Meanwhile, the post hoc LSD test between concentrations of 5% - 12.5% with 0.2% chlorhexidine showed a significant difference in antibacterial power (p<0.05). This indicates a stronger antibacterial activity at a concentration of 5% - 12.5% compared to 0.2% chlorhexidine.Conclusion: EENL mouthwash effective against the growth of Streptococcus sp
Negative correlation of Modified Sperm Stress Test with Leukocyte in Sperm but not with the sperm concentration and motility Dicky Moch Rizal; Geraldo Laurus; Nandia Septiyorini
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.15222

Abstract

Background: Sperm analysis is an initial detection procedure of male infertility recommended by the WHO. Sperm motility as one of the important parameters in sperm analysis has a close correlation with various determinants of male infertility and the success of Assissted Reproductive Technology (ART). Several laboratory assays were carried out as a complement to sperm analysis to describe the sperm ability for fertilization. One of the assays was the modified sperm stress test (MOST) to predict oocyte fertilization in In-Vitro Fertilization (IVF).Objective: This study aimed to assess the correlation between MOST ratio and sperm parameters in infertile men.Methods: This study is a descriptive-analytic study by taking sperm analysis and MOST data from 864 males with infertility problems who went to an infertility clinic, Sadewa IVF, Yogyakarta, Indonesia from May 2015 to November 2020.Results: The mean sperm volume and concentration of the 864 patients were 3.04 mL and 35.74 x 106 / mL respectively. The mean forward motility and normal morphology were 47.93% and 2.07% respectively. The mean leukocyte cell number was 0.84%, and the mean MOST ratio was 0.51. The results showed a positive correlation between sperm concentration (r = 0.235) and motility (r = 0.302) with the MOST ratio. Meanwhile, the leukocyte parameter negatively correlated with the MOST ratio (r = -0.076) and no correlation between other sperm parameters to the MOST ratio.Conclusion:There is a positive correlation between sperm concentration and motility with the MOST ratio, and a negative correlation between the number of leukocytes and the MOST ratio. This result supports the findings of previous studies regarding the importance of sperm motility examination in the success of ART. To the best of our knowledge, this is the first study to assess the MOST in male infertility patients with the big sample size.
The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork Sandi Muslim; Arnila Novitasari Saubig; Riski Prihatningtias; A. Kentar Arimadyo Sulakso; Fifin Luthfia Rahmi
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.13817

Abstract

Background: The extracellular matrix (ECM) in the trabecular meshwork (TM) may play a role in the steroid-induced aqueous humor outflow resistance pathophysiology. Topical steroids such as fluorometholone can cause elevated intraocular pressure. Increased synthesis and accumulation of TM ECM proteins due to steroids result in TM dysfunction and elevated intraocular pressure.Purpose: This study aims to analyze the effect of duration of administration and discontinuation of fluorometholone eye drops on the thickness of the TM ECM of Wistar rats.Methods: This study was an experimental study with a posttest-only control group design. The total sample of 25 rats was divided into 5 groups: Treatment 1 was given topical fluorometholone for 4 weeks and stopped for 4 weeks. Treatment 2 was given fluorometholone for 6 weeks and stopped for 4 weeks. Control 1 was given topical fluorometholone for 4 weeks, Control 2 was given topical fluorometholone for 6 weeks, and the negative control was used as a baseline. TM ECM examination was assessed using histopathological score grading. This study used the Mann-Whitney test and the Kruskal-Wallis comparative hypothesis test.Results: The difference in ECM thickness was not statistically significant either between treatment group 1 and treatment group 2 (p=1.000) or control group 1 (p=0,1000). There was a significant change in the thickness of the TM ECM in treatment group 1, treatment 2, control 1, control 2 compared to the negative control (p=0.003, p=0.003, p=0.003, p=0.004).Conclusion: TM ECM thickness did not return to normal thickness after administration of fluorometholone eye drops one drop 4 times a day for 4 weeks and 6 weeks followed by discontinuation of the drug for 4 weeks. The thickness of the TM ECM was thicker in experimental rats with a longer duration of administration of fluorometholone eye drops.
Correlation between Interleukin-17 Levels with C-Reactive Protein and Neutrophil Lymphocyte Ratio in Sepsis Dwi Retnoningrum; Muchlis Achsan Udji Sofro; Setyo Gundi Pramudo; Satrio Adi Wicaksono; Andaru Dahesihdewi
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.15323

Abstract

Background: Sepsis is a condition in systemic infection associated with organdysfunction. Interleukin-17 is a pro-inflammatory cytokine produced by Th-17 cells. C-reactive protein and neutrophil-lymphocyte ratio (NLR) have been widely used asmarkers of inflammation. The relationship between IL-17 as a proinflammatorycytokine with CRP and NLR has not been reported.Objective: This study aims to prove the correlation between IL-17 with CRP and NLRin septic patients.Methods: Analytical observational study with a cross-sectional approach was carriedout on 40 septic patients in July–December 2020. IL-17 levels were obtained by theELISA method while CRP levels were obtained using the immunoturbidimetric method.NLR was the result of dividing the absolute number of neutrophils and lymphocytesfrom the automatic hematology analyzer. Correlation between variables was performedusing Spearman correlation test.Results: The median levels of IL-17, CPR, and NLR were 363.55 (11.4-1695.80)pg/mL, 13.25 (0.43-53.87) mg/L, and 12.00 (2.26–48.5), respectively. The Spearmancorrelation test between IL-17 levels and CRP obtained p = 0.019, r = 0.37, and NLR p= 0.425, r = 0.13.Conclusion: There is a weak positive correlation between IL-17 levels and CRP inseptic patients. There is no correlation between IL-17 levels and NLR in septic patients.

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