cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Kuswanto
Contact Email
rjls@ub.ac.id
Phone
+62341 575825
Journal Mail Official
rjls@ub.ac.id
Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia
Location
Kota malang,
Jawa timur
INDONESIA
Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol 10, No 3 (2023)" : 5 Documents clear
The Influence of Seed Biopriming with Rhizobacteria on The Control of Black Rot Disease by Xanthomonas campestris Pathogens Rahman, Fakih Nur; Aini, Luqman Qurata; Syib`li, Muhammad Akhid
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.2

Abstract

The use of chemical pesticides in agriculture has negative impacts on the environment and health and leads to pathogen resistance. As an alternative, microorganisms such as PGPR or PGPF can enhance plant resilience and growth. Biopriming can trigger systemic resistance, improve nutrient uptake, and protect plants from pathogen infection. This study aims to evaluate the potential of biopriming and the effects of rhizobacterial application on plants. There are three main tests: the pathogenicity test of Xanthomonas campestris, the rhizobacterial test on cabbage seeds and seedlings, and the effectiveness of rhizobacteria in suppressing black rot disease in cabbage. Rhizobacterial application increased peroxidase enzyme and total phenol levels in plants, as well as plant height and leaf number. Rhizobacteria also improved cabbage seed germination and suppressed plant diseases. In conclusion, all tested rhizobacteria induced resistance in cabbage plants by increasing peroxidase enzyme and total phenol levels. Rhizobacterium TO9 (Clostridium sp.) was the most effective in enhancing plant resistance. Rhizobacteria also stimulated cabbage growth. Further research is needed to understand the mechanisms and effectiveness of rhizobacteria under different agricultural conditions.
Comparison of Primer Types to Produce Fungal DNA Fragment from Garlic Plant Root Samples Dewi, Fibrianti Shinta; Abadi, Abdul Latief; Syib'li, Muhammad Akhid
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.3

Abstract

Fusarium wilt disease caused by Fusarium oxysporum f.sp cepae is a widespread and dangerous diseases in garlic commodities. The combination of AMF and Bacillus amyloliquefaciens can be an effective solution to reduce losses due to attacks by these pathogens. The PCR technique is initial method that has proven to be significant in detecting fungal target DNA so that the effectiveness of biological agents can be determined. The aim of this study is to compare the types of primers that are effective for use in detecting fungal groups in PCR. This research was conducted in October 2023 - November 2024. The types of primers compared were ITS1-F/ITS4-R and LSU200A-F/LSU476-R with spectophotometry and fluometry quantification. The results show that ITS1-F/ITS4-R forms DNA band with a size of 700 bp, while LSU200A-F/LSU476-R had a size of 290-300 bp. The electropherogram also showed differences in DNA distribution which confirmed the results on agarose more specifically in the range 19-12,999 bp. In general, these results prove that the two types of primers have different abilities in fragmenting target DNA. This knowledge can help in detecting DNA at an early stage which can be used in various analyzes that can help in integrated plant disease management.
The Use of Beneficial Microbes Tolerant of the Pyraclostrobin Fungicide, in Controlling the Pathogen Colletotrichum, the Cause of Anthracnose Disease in Chili Plants Tubagus, Satria Wahyu; Syib’li, Muhammad Akhid; Abadi, Abdul Latief
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.1

Abstract

Global production of chilies is significantly influenced by various biotic and abiotic factors. anthracnose is likely the main inhibiting factor reducing chili productivity in various countries. The use chemicals still formed the basis of disease control. Trichoderma sp. and FB1 bacterial is one such tolerant beneficial microbe belonging to the fungus and bacteria group that can survive under chemical pressure and exhibit antagonistic effects against the pathogen Colletotrichum sp. by the in vitro assay, it was found that in the antagonistic of Colletotrichum sp. with bacteria FB1 and Trichoderma sp., there was a significant difference, that the growth of Colletotrichum sp. could be inhibited by 69%. In the compatibility assay of bacteria FB1 and Trichoderma sp., no significant antagonistic response was found from both beneficial microbes. In the fungicide tolerance test of all isolates, it was found that beneficial microbes could still grow on growth media with a mixture of pyraclostrobin fungicide at a dose of 0.25 ul/ml. FTIR analysis showed the functional groups of the secondary metabolites of both the antagonistic bacteria and the antagonistic fungi had very high similarity in O-H and C=O groups. However, further research is needed on the mechanism of the secondary metabolites of the beneficial microbes involved in the inhibition of the pathogen Colletotrichum sp. 
Pathogenicity Test and Molecular Characterization of Colletotrichum spp. Fungi Causing Apple Fruit Rot Disease in Malang Regency Maknunin, Lu'lu'il; Abadi, Abdul Latief; Syib'li, Muhammad Akhid
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.4

Abstract

Apple (Malus sylvestris Mill) is a key commodity in Malang Regency, Indonesia, because of its role in promoting the agrotourism sector. But fruit rot caused by Colletotrichum spp. poses significant threats to production and its existence. This study aims to identify the causal agents of fruit rot in apple orchards in Pujon District, Malang, using morphological and molecular approaches. Four isolates suspected to be Colletotrichum spp. were obtained from symptomatic apple fruits and characterized through macroscopic, microscopic, and molecular methods, including ITS sequencing. Pathogenicity tests revealed that all isolates induced symptoms on healthy apples, with Colletotrichum siamense confirmed as the primary pathogen via BLAST analysis. These findings emphasize the need for accurate morphological and molecular identification to develop effective management strategies. Further research is recommended to examine the diversity of Colletotrichum species in Malang Regency to inform broader disease control strategies.
Biodiversity and Distribution Rice Arthropods in Malang Indonesia Istiqomah, Rina Nur; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.5

Abstract

Climate change is closely related to changes in abiotic conditions. These changes could have an impact on agricultural cultivation and affect the existence of agricultural arthropods. Different environmental conditions will influence the distribution patterns, richness and diversity of arthropods living in cultivated land. The research was conducted at 8 rice cultivation locations in Greater Malang. Observation of arthropods using pitfall and yellow pan traps, with a diagonal pattern. The total arthropods obtained were 3,937 individuals, 39 families, 46 genera and 48 species. The highest predator species found were Micraspis discolor, the herbivore Nilaparvata lugens, other insect is Culista incidens, and the predator is Drynus baegamensis. The highest index analysis for diversity in Lowokwaru H'= 3,075 in the high category, evenness in Karangploso E=0,864 in the high category and dominance in Pagelaran D=0,179 in the low category. PCA analysis of species composition divided into 2 habitat types, lowland and medium land groups. Species richness increased with increasing observation locations. Environmental factors will bring in species that are suitable for the habitat. Further research needs to be carried out regarding ecological variables or environmental factors, which affect the patterns composition and species richness of arthropods.

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2023 2023


Reset
Filter By Issues
All Issue Vol 11, No 1 (2024) Vol 10, No 3 (2023) Vol 10, No 2 (2023) Vol 10, No 1 (2023) Vol 9, No 3 (2022) Vol 9, No 2 (2022) Vol 9, No 1 (2022) Vol 8, No 3 (2021) Vol 8, No 2 (2021) Vol 8, No 1 (2021) Vol 7, No 3 (2020) Vol 7, No 2 (2020) Vol 7, No 1 (2020) Vol 6, No 3 (2019) Vol 6, No 2 (2019) Vol 6, No 1 (2019) Vol 5, No 3 (2018) Vol 5, No 2 (2018) Vol 5, No 1 (2018) Vol 4, No 3 (2017) Vol 4, No 2 (2017) Vol 4, No 1 (2017) Vol 3, No 3 (2016) Vol 3, No 2 (2016) Vol 3, No 1 (2016) Vol 2, No 3 (2015) Vol 2, No 2 (2015) Vol 2, No 1 (2015) Vol 1, No 1 (2014) More Issue